Multimodal bioorthogonal small molecule probes play a pivotal role in drug-focused biomedical research.However,existing drug tracking and imaging techniques face obstacles in living organisms,hindering precise drug lo...Multimodal bioorthogonal small molecule probes play a pivotal role in drug-focused biomedical research.However,existing drug tracking and imaging techniques face obstacles in living organisms,hindering precise drug localization and target protein capture.Herein,we introduced a multimodal probe named 1-(azidomethyl)pyrene-4,5–dione(AMPD).The probe incorporates adjacent dione structures at the pyrene core.AMPD selectively interacts with oxygen-rich alkene-labeled drug molecules under ice-blue LED light exposure,producing specific fluorescence emission and enabling in vivo tracking and flow cytometry sorting.A methyl azide group was also introduced at the pyrene core to help efficiently enrich target proteins via click chemistry with alkyne-functionalized beads.AMPD demonstrates exceptional biocompatibility,rendering it highly suitable for visual photo-triggered tracking studies.Combined with metabolic labeling using an oxygen-rich alkene-tagged drug molecule probe,AMPD is effective for live animal,tissue,cellular,and in-gel imaging,as well as target protein identification through magnetic capture.With its versatile capabilities,AMPD enhances our comprehension of drug-target interactions at the in vivo level and expedites the process of drug discovery.展开更多
Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide.The mechanisms of carcinogenesis,cancer invasion,and metastasis remain unclear.Thus,the developm...Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide.The mechanisms of carcinogenesis,cancer invasion,and metastasis remain unclear.Thus,the development of a novel approach for cancer detection is urgent,and real-time monitoring is crucial in revealing its underlying biological mechanisms.With the optical and chemical advantages of quantum dots(QDs),QD-based nanotechnology is helpful in constructing a biomedical imaging platform for cancer behavior study.This review mainly focuses on the application of QD-based nanotechnology in cancer cell imaging and tumor microenvironment studies both in vivo and in vitro,as well as the remaining issues and future perspectives.展开更多
基金supported by the National Key Research and Development Program of China(Nos.2022YFC3500800 and2022YFC3500805)。
文摘Multimodal bioorthogonal small molecule probes play a pivotal role in drug-focused biomedical research.However,existing drug tracking and imaging techniques face obstacles in living organisms,hindering precise drug localization and target protein capture.Herein,we introduced a multimodal probe named 1-(azidomethyl)pyrene-4,5–dione(AMPD).The probe incorporates adjacent dione structures at the pyrene core.AMPD selectively interacts with oxygen-rich alkene-labeled drug molecules under ice-blue LED light exposure,producing specific fluorescence emission and enabling in vivo tracking and flow cytometry sorting.A methyl azide group was also introduced at the pyrene core to help efficiently enrich target proteins via click chemistry with alkyne-functionalized beads.AMPD demonstrates exceptional biocompatibility,rendering it highly suitable for visual photo-triggered tracking studies.Combined with metabolic labeling using an oxygen-rich alkene-tagged drug molecule probe,AMPD is effective for live animal,tissue,cellular,and in-gel imaging,as well as target protein identification through magnetic capture.With its versatile capabilities,AMPD enhances our comprehension of drug-target interactions at the in vivo level and expedites the process of drug discovery.
基金supported by the Academic Award for Excellent Ph.D.Candidates Funded by the Ministry of Education of China(No.5052011303014)the National Natural Science Foundation of China(No.81171396)+1 种基金the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(Nos.20621502 and 20921062)the Fundamental Research Funds for the Central Universities of Ministry of Education of China(No.4103005).
文摘Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide.The mechanisms of carcinogenesis,cancer invasion,and metastasis remain unclear.Thus,the development of a novel approach for cancer detection is urgent,and real-time monitoring is crucial in revealing its underlying biological mechanisms.With the optical and chemical advantages of quantum dots(QDs),QD-based nanotechnology is helpful in constructing a biomedical imaging platform for cancer behavior study.This review mainly focuses on the application of QD-based nanotechnology in cancer cell imaging and tumor microenvironment studies both in vivo and in vitro,as well as the remaining issues and future perspectives.
