A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrod...A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrodes. The dual Pt working electrodes with disk shape and the surrounding ring shaped counter electrode were fabricated by thin film technology, which were integrated onto the same microchip. The response of the designed biosensor for glucose and galactose were linear up to 6.0 mmol/L and 3.5 mmol/L with sensitivities of 0.3 mA/mmol/L and 0.12 mA/mmol/L, respectively. No cross-talking effect was observed.展开更多
An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber...An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.展开更多
To accomplish the reliability analyses of the correlation of multi-analytical objectives,an innovative framework of Dimensional Synchronous Modeling(DSM)and correlation analysis is developed based on the stepwise mode...To accomplish the reliability analyses of the correlation of multi-analytical objectives,an innovative framework of Dimensional Synchronous Modeling(DSM)and correlation analysis is developed based on the stepwise modeling strategy,cell array operation principle,and Copula theory.Under this framework,we propose a DSM-based Enhanced Kriging(DSMEK)algorithm to synchronously derive the modeling of multi-objective,and explore an adaptive Copula function approach to analyze the correlation among multiple objectives and to assess the synthetical reliability level.In the proposed DSMEK and adaptive Copula methods,the Kriging model is treated as the basis function of DSMEK model,the Multi-Objective Snake Optimizer(MOSO)algorithm is used to search the optimal values of hyperparameters of basis functions,the cell array operation principle is adopted to establish a whole model of multiple objectives,the goodness of fit is utilized to determine the forms of Copula functions,and the determined Copula functions are employed to perform the reliability analyses of the correlation of multi-analytical objectives.Furthermore,three examples,including multi-objective complex function approximation,aeroengine turbine bladeddisc multi-failure mode reliability analyses and aircraft landing gear system brake temperature reliability analyses,are performed to verify the effectiveness of the proposed methods,from the viewpoints of mathematics and engineering.The results show that the DSMEK and adaptive Copula approaches hold obvious advantages in terms of modeling features and simulation performance.The efforts of this work provide a useful way for the modeling of multi-analytical objectives and synthetical reliability analyses of complex structure/system with multi-output responses.展开更多
Biomolecules play important roles in physiological functions and pharmacological characteristics of human body.Uric acid(UA)is the end product of purine.Dopamine(DA)is a neurotransmitter of catecholamine group.L-trypt...Biomolecules play important roles in physiological functions and pharmacological characteristics of human body.Uric acid(UA)is the end product of purine.Dopamine(DA)is a neurotransmitter of catecholamine group.L-tryptophan is an essential amino acid that can be metabolized to neuroactive substances.Pyridoxine is a water-soluble vitamin playing an important role in nervous system.The abnormalities in their concentration levels led to a wide range of signifi cant mental and physical illnesses.Thus,electrochemical analysis of these analytes on an array system would be benefi cial from clinical or scientifi c points of view.This work was aimed at the development of practical sensor array for determination of multiple analytes on a single sensing platform using individually addressable microelectrodes.The occurrence of adsorption–desorption phenomenon on the surface of palladium microelectrode array(Pd MEA)printed on the silicon wafer through photolithography was exploited for electro-oxidation of UA,DA,L-tryptophan and pyridoxine.The sensing of electroactive UA was done using carbon nanotubes(CNTs)grown Pd MEA as a working electrode,while selectivity for other analytes was achieved by the modifi cation of CNTs/Pd MEA through electrodeposition of poly(L-lysine)(poly(L-lysine)/CNTs/Pd MEA)for DA sensing,poly(L-arginine)(poly(L-arginine)/CNTs/Pd MEA)for L-tryptophan sensing and reduced graphene oxide(rGO/CNTs/Pd MEA)for pyridoxine sensing.The electrochemical diff erential pulse voltammetry(DPV)analyses reveal excellent linearity in the concentration ranges of 50–6000μmol/L,2–8000μmol/L,20–15,000μmol/L,and 10–5000μmol/L with detection limits of 15.0,0.5,10.0,and 1.0μmol/L for UA,DA,L-tryptophan,and pyridoxine,respectively.The proposed multiple analytes sensor has shown very high sensitivities of 140,9580,2280,and 940μA·(μmol·L^(-1))^(-1)·cm^(-2)for UA,DA,L-tryptophan,and pyridoxine sensing,respectively.Further,accuracy and reliability of the fabricated sensor were also tested in real samples.展开更多
文摘A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrodes. The dual Pt working electrodes with disk shape and the surrounding ring shaped counter electrode were fabricated by thin film technology, which were integrated onto the same microchip. The response of the designed biosensor for glucose and galactose were linear up to 6.0 mmol/L and 3.5 mmol/L with sensitivities of 0.3 mA/mmol/L and 0.12 mA/mmol/L, respectively. No cross-talking effect was observed.
基金Shanghai Rising Star Project of Shanghai Science and Technology Committee (No. 04QMX1443) the Science Foundation of Shanghai Education Commission and Applied Chemistry Key Laboratory Foundation of Shanghai Normal University.
文摘An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.
基金co-supported by the National Natural Science Foundation of China(Nos.52405293,52375237)China Postdoctoral Science Foundation(No.2024M754219)Shaanxi Province Postdoctoral Research Project Funding,China。
文摘To accomplish the reliability analyses of the correlation of multi-analytical objectives,an innovative framework of Dimensional Synchronous Modeling(DSM)and correlation analysis is developed based on the stepwise modeling strategy,cell array operation principle,and Copula theory.Under this framework,we propose a DSM-based Enhanced Kriging(DSMEK)algorithm to synchronously derive the modeling of multi-objective,and explore an adaptive Copula function approach to analyze the correlation among multiple objectives and to assess the synthetical reliability level.In the proposed DSMEK and adaptive Copula methods,the Kriging model is treated as the basis function of DSMEK model,the Multi-Objective Snake Optimizer(MOSO)algorithm is used to search the optimal values of hyperparameters of basis functions,the cell array operation principle is adopted to establish a whole model of multiple objectives,the goodness of fit is utilized to determine the forms of Copula functions,and the determined Copula functions are employed to perform the reliability analyses of the correlation of multi-analytical objectives.Furthermore,three examples,including multi-objective complex function approximation,aeroengine turbine bladeddisc multi-failure mode reliability analyses and aircraft landing gear system brake temperature reliability analyses,are performed to verify the effectiveness of the proposed methods,from the viewpoints of mathematics and engineering.The results show that the DSMEK and adaptive Copula approaches hold obvious advantages in terms of modeling features and simulation performance.The efforts of this work provide a useful way for the modeling of multi-analytical objectives and synthetical reliability analyses of complex structure/system with multi-output responses.
基金The author AS(Chem./2018-19/RET/Sept.18term/1/4809),SA(Chem./2019-2020/RET-2/Sept.19-term/1/975)and SS(Chem.-22/RET-Ex./July-22-term/26/564)are grateful to UGC,New Delhi and IOE,BHU for funding their doctoral researchThe financial supports received from Scheme for Promotion of Academic and Research Collaboration(SPARC-6019)MHRD,India,UKIERI,UK and IOE incentive grant for faculty(Scheme Number-6031)。
文摘Biomolecules play important roles in physiological functions and pharmacological characteristics of human body.Uric acid(UA)is the end product of purine.Dopamine(DA)is a neurotransmitter of catecholamine group.L-tryptophan is an essential amino acid that can be metabolized to neuroactive substances.Pyridoxine is a water-soluble vitamin playing an important role in nervous system.The abnormalities in their concentration levels led to a wide range of signifi cant mental and physical illnesses.Thus,electrochemical analysis of these analytes on an array system would be benefi cial from clinical or scientifi c points of view.This work was aimed at the development of practical sensor array for determination of multiple analytes on a single sensing platform using individually addressable microelectrodes.The occurrence of adsorption–desorption phenomenon on the surface of palladium microelectrode array(Pd MEA)printed on the silicon wafer through photolithography was exploited for electro-oxidation of UA,DA,L-tryptophan and pyridoxine.The sensing of electroactive UA was done using carbon nanotubes(CNTs)grown Pd MEA as a working electrode,while selectivity for other analytes was achieved by the modifi cation of CNTs/Pd MEA through electrodeposition of poly(L-lysine)(poly(L-lysine)/CNTs/Pd MEA)for DA sensing,poly(L-arginine)(poly(L-arginine)/CNTs/Pd MEA)for L-tryptophan sensing and reduced graphene oxide(rGO/CNTs/Pd MEA)for pyridoxine sensing.The electrochemical diff erential pulse voltammetry(DPV)analyses reveal excellent linearity in the concentration ranges of 50–6000μmol/L,2–8000μmol/L,20–15,000μmol/L,and 10–5000μmol/L with detection limits of 15.0,0.5,10.0,and 1.0μmol/L for UA,DA,L-tryptophan,and pyridoxine,respectively.The proposed multiple analytes sensor has shown very high sensitivities of 140,9580,2280,and 940μA·(μmol·L^(-1))^(-1)·cm^(-2)for UA,DA,L-tryptophan,and pyridoxine sensing,respectively.Further,accuracy and reliability of the fabricated sensor were also tested in real samples.
