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U-Fish:一种基于密度图回归的鱼群计数方法
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作者 尹莉娜 陈晓燕 +2 位作者 黄芳 李渝海 熊雄 《南京农业大学学报》 北大核心 2026年第1期214-224,共11页
[目的]针对现有鱼群计数方法在实际应用中遇到的水面反光、鱼群重叠、模型细节丢失和模型参数量大等问题,本文提出了一种基于密度图回归的鱼群计数方法。[方法]网络结构方面,构建U-Fish网络结构,实现鱼群精确计数,同时优化模型参数,减... [目的]针对现有鱼群计数方法在实际应用中遇到的水面反光、鱼群重叠、模型细节丢失和模型参数量大等问题,本文提出了一种基于密度图回归的鱼群计数方法。[方法]网络结构方面,构建U-Fish网络结构,实现鱼群精确计数,同时优化模型参数,减轻计算负担;损失函数方面,引入多尺度密度水平一致性损失和结构相似性损失,实现对欧几里得距离损失函数的优化,提高密度图生成质量;图像增强方面,使用混合图像增强,利用CLAHE图像增强方法优化图像质量,减轻图像反光问题,并混合原图与增强后的图像进行计数。[结果]试验结果表明,U-Fish网络的MAE为4.05,RMSE为5.48,与主流的计数模型相比,取得了最优的精度,同时保持了较小的参数量。[结论]U-Fish模型能够有效解决现有鱼群计数方法中精度有限和模型计算负担过重的问题。 展开更多
关键词 鱼群计数 密度图回归计数 图像增强 深度学习
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Carbon Footprint and Economic Analysis of LNG-fueled Fishing Vessel Using Real Engine Performance Simulation
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作者 Momir Sjerić Maja Perčić +1 位作者 Ivana Jovanović Nikola Vladimir 《哈尔滨工程大学学报(英文版)》 2026年第1期259-276,共18页
Analysis of the environmental and economic performance of fishing vessels has received limited attention compared with other ship types despite their notable contribution to global greenhouse gas(GHG)emissions.This st... Analysis of the environmental and economic performance of fishing vessels has received limited attention compared with other ship types despite their notable contribution to global greenhouse gas(GHG)emissions.This study evaluates the carbon footprint(CF)and economic viability of a liquefied natural gas(LNG)-fueled fishing vessel,using real engine operation simulations to provide precise and dynamic evaluation of fuel consumption and GHG emissions.Operational profiles are obtained through the utilization of onboard monitoring systems,whereas engine performance is simulated using the 1D/0D AVL Boost^(TM)model.Life cycle assessment(LCA)is conducted to quantify the environmental impact,whereas life cycle cost assessment(LCCA)is performed to analyze the profitability of LNG as an alternative fuel.The potential impact of the future fuel price uncertainties is addressed using Monte Carlo simulations.The LCA findings indicate that LNG has the potential to reduce the CF of the vessel by 14%to 16%,in comparison to a diesel power system configuration that serves as the baseline scenario.The LCCA results further indicate that the total cost of an LNG-powered ship is lower by 9.5%-13.8%,depending on the share of LNG and pilot fuels.This finding highlights the potential of LNG to produce considerable environmental benefits while addressing economic challenges under diverse operational and fuel price conditions. 展开更多
关键词 1D/0D simulation Carbon footprint fishing vessels Life cycle assessment Life cycle cost assessment Liquefied natural gas
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基于荧光原位杂交(FISH)技术的假俭草染色体鉴定和核型分析
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作者 李建建 王浩然 +4 位作者 张源 徐夕雯 李晓慧 张苓 郭海林 《植物资源与环境学报》 北大核心 2025年第1期33-41,共9页
以假俭草(Eremochloa ophiuroides(Munro)Hack.)品种‘赣北’(‘Ganbei’)基因组序列数据为基础,通过生物信息学手段挖掘和设计了假俭草染色体特异的单拷贝寡核苷酸(oligo)探针库,同时结合端粒重复序列和rDNA重复序列探针,在假俭草根尖... 以假俭草(Eremochloa ophiuroides(Munro)Hack.)品种‘赣北’(‘Ganbei’)基因组序列数据为基础,通过生物信息学手段挖掘和设计了假俭草染色体特异的单拷贝寡核苷酸(oligo)探针库,同时结合端粒重复序列和rDNA重复序列探针,在假俭草根尖细胞有丝分裂中期染色体上进行了系列荧光原位杂交(FISH)试验。结果显示:假俭草染色体核型公式为2n=2x=18(2SAT),利用5S rDNA和18S rDNA重复序列探针可识别出第5和第4号染色体,且第4号染色体18S rDNA富集区段易出现或存在断裂现象;设计的假俭草染色体特异单拷贝oligo探针可识别出第2、第6和第9号染色体,进一步结合rDNA重复序列探针结果还发现第4号染色体与第8号染色体间发生了染色体易位。综合分析认为,假俭草染色体核型存在不稳定性,且其基因组在进化过程中发生了染色体结构重排。本研究建立的基于重复序列和单拷贝oligo探针的FISH技术体系,可为假俭草染色体的准确识别和变异鉴定提供新方法,观察到的假俭草染色体核型特征可为假俭草高质量基因组图谱的构建提供细胞学证据。 展开更多
关键词 假俭草 染色体 重复序列 寡核苷酸(oligo) 荧光原位杂交(fish) 核型分析
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NGS、RT-PCR和FISH在非小细胞肺癌ALK检测中的比较研究
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作者 岳林 白雪峰 +8 位作者 项鹏程 虞飞 白建辉 冯立文 高洋 武小燕 刘恩 丁海麦 王焱宏 《包头医学院学报》 2025年第10期71-76,共6页
目的:准确的间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)重排检测在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者ALK抑制剂(anaplastic lymphoma kinase tyrosine kinase inhibitors,ALK-TKI)靶向治疗中至关重要。目前,... 目的:准确的间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)重排检测在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者ALK抑制剂(anaplastic lymphoma kinase tyrosine kinase inhibitors,ALK-TKI)靶向治疗中至关重要。目前,医院实验室所使用的检测方法有基于逆转录PCR(reverse transcription-PCR,RT-PCR)、荧光原位杂交(fluorescence in situ hybridization,FISH)和二代测序(next generation sequencing,NGS)。本文评估了三种方法检测ALK重排的性能,为ALK的精确检测提供理论支持。方法:选取38例ALK阳性患者,分别用RT-PCR、FISH和NGS对样本进行ALK重排检测,评估三种方法在ALK检测中的一致性。再分别分析这三种ALK方法与ALK-TKI治疗效果的一致性。结果:分别用RT-PCR、FISH和NGS方法检测38例得到的ALK重排阳性率分别是89.47%、86.84%和97.37%,其中,NGS检测出3例罕见ALK重排。RT-PCR与FISH方法在检查ALK重排结果有较强的一致性(Kappa=0.623,P<0.05);NGS与RT-PCR、FISH检测ALK重排没有一致性(Kappa=-0.044和0.303)。该三组方法定义的ALK阳性病例经ALK-TKI治疗22个月的存活数,统计分析结果显示差异无统计学意义(P>0.05)。结论:与RT-PCR和FISH相比,NGS方法显示出更高的阳性检出率,特别是在罕见的ALK重排中具有更强的检测能力,因此,本研究倡导在实验条件满足的情况下采用NGS来检测ALK重排,能有效提升NSCLC患者的治疗效果和预后评估。 展开更多
关键词 间变性淋巴瘤激酶 非小细胞肺癌 逆转录PCR 荧光原位杂交 二代测序
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Current status and diversity of fish resources in the Pinglu Canal Century Project 被引量:1
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作者 LU Jia TANG Wei +3 位作者 WANG Li XU Shengxian ZHANG Peng YANG Shengfa 《水利水电技术(中英文)》 北大核心 2025年第4期131-144,共14页
[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of... [Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of fish resources and the characteristics of their diversity is crucial for the ecological management of the Pinglu Canal.[Methods]During the spring and autumn in 2021 and 2022,a survey of fish resources and species diversity in the Pinglu Canal was conducted using multi-mesh gill nets.A total of 125 fish species were collected,belonging to 10 orders,34 families,and 89 genera.[Results]The result showed that the Pinglu Canal contained three nationally protected Class II species,two endemic species of the Qinjiang River,three anadromous/migratory species,and eight invasive species,accounting for 2.4%,1.6%,2.4%,and 6.4%of the total species,respectively.The fish community primarily consisted of mid-and bottom-dwelling,adhesive-egg-laying,and omnivorous species.The Shannon-Wiener,Simpson,Margalef,and Pielou indices of the fish community in the Pinglu Canal ranged from 2.347 to 2.757,0.081 to 0.151,3.493 to 4.382,and 0.812 to 0.892,respectively.These indices showed relatively uniform distribution across different river reaches.[Conclusion]The result indicate that the fish community structure in the Pinglu Canal is relatively uniform.The reach from the Yujiang River to the Shaping River shows higher stability,while other river reaches experience moderate or severe disturbances.This study provides supplementary baseline data on the fish community structure in the Pinglu Canal and explores the potential impact of inter-basin connectivity on fish resources,aiming to provide a scientific basis for habitat restoration assessments after the channel straightening project. 展开更多
关键词 Pinglu Canal Project inter-basin connectivity fish resources community structure BIODIVERSITY influencing factors
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Pathfinder:Deep Reinforcement Learning-Based Scheduling for Multi-Robot Systems in Smart Factories with Mass Customization 被引量:1
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作者 Chenxi Lyu Chen Dong +3 位作者 Qiancheng Xiong Yuzhong Chen Qian Weng Zhenyi Chen 《Computers, Materials & Continua》 2025年第8期3371-3391,共21页
The rapid advancement of Industry 4.0 has revolutionized manufacturing,shifting production from centralized control to decentralized,intelligent systems.Smart factories are now expected to achieve high adaptability an... The rapid advancement of Industry 4.0 has revolutionized manufacturing,shifting production from centralized control to decentralized,intelligent systems.Smart factories are now expected to achieve high adaptability and resource efficiency,particularly in mass customization scenarios where production schedules must accommodate dynamic and personalized demands.To address the challenges of dynamic task allocation,uncertainty,and realtime decision-making,this paper proposes Pathfinder,a deep reinforcement learning-based scheduling framework.Pathfinder models scheduling data through three key matrices:execution time(the time required for a job to complete),completion time(the actual time at which a job is finished),and efficiency(the performance of executing a single job).By leveraging neural networks,Pathfinder extracts essential features from these matrices,enabling intelligent decision-making in dynamic production environments.Unlike traditional approaches with fixed scheduling rules,Pathfinder dynamically selects from ten diverse scheduling rules,optimizing decisions based on real-time environmental conditions.To further enhance scheduling efficiency,a specialized reward function is designed to support dynamic task allocation and real-time adjustments.This function helps Pathfinder continuously refine its scheduling strategy,improving machine utilization and minimizing job completion times.Through reinforcement learning,Pathfinder adapts to evolving production demands,ensuring robust performance in real-world applications.Experimental results demonstrate that Pathfinder outperforms traditional scheduling approaches,offering improved coordination and efficiency in smart factories.By integrating deep reinforcement learning,adaptable scheduling strategies,and an innovative reward function,Pathfinder provides an effective solution to the growing challenges of multi-robot job scheduling in mass customization environments. 展开更多
关键词 Smart factory CUSTOMIZATION deep reinforcement learning production scheduling multi-robot system task allocation
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Checklist of marine fishes in the Beibu Gulf:fish classification,resource protection,and biodiversity challenge 被引量:1
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作者 Zhisen LUO Murong YI +9 位作者 Xiaodong YANG Xiao CHEN Jinxi WANG Changping JIANG Fengming LIU Konglan LUO Xiongbo HE Hung-Du LIN Bin KANG Yunrong YAN 《Journal of Oceanology and Limnology》 2025年第1期232-247,共16页
Marine fish diversity in the Beibu Gulf from 2014 to 2022 was analyzed,using various methods including at-sea fisheries resource surveys,fishing port surveys,underwater survey techniques,and data from literature,books... Marine fish diversity in the Beibu Gulf from 2014 to 2022 was analyzed,using various methods including at-sea fisheries resource surveys,fishing port surveys,underwater survey techniques,and data from literature,books,and databases,from which 1059 fish species across 40 orders,166 families,and 503 genera were identified.Among them,Acanthuriformes displayed the highest diversity,followed by Carangiformes and Perciformes.Notably,eight alien species were found in the Beibu Gulf.The International Union for Conservation of Nature(IUCN)Red List assessment revealed 51threatened species,primarily cartilaginous fish.Taxonomic changes affected 88.70%of species due to classification adjustments,misidentifications,and junior synonymy.This study contributed new data of the gulf,including one additional order,six new families,34 new genera,and 81 new species,emphasizing the dynamic nature of marine ecosystems.Fish diversity in the gulf was relatively lower than the broader South China Sea,attributed to limited habitats and survey methods.Different survey methods,such as gill nets,trap nets,and underwater cameras,yielded varying results.Ocean currents may have transported deep-sea species into the Beibu Gulf,leading to accidental records.Research gaps exist in unexplored areas,warranting further investigation.Therefore,the Beibu Gulf hosts significant marine biodiversity,but taxonomic challenges and undiscovered species require new research and conservation efforts. 展开更多
关键词 northwestern South China Sea new record taxonomic change fish diversity survey method
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Environmental DNA assessment of fish diversity, distribution and niche characteristics in Zhutuo spawning ground in the upper reaches of the Yangtze River
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作者 LU Jia WANG Li +3 位作者 LI Ruijiao YANG Jin ZHANG Peng YANG Shengfa 《水利水电技术(中英文)》 北大核心 2025年第S1期454-467,共14页
[Objective]Implementation of the Ten-Year Fishing Ban policy may alter fish diversity and niche characteristics of dominant species in spawning grounds within the National Nature Reserve for Rare and Endemic Fish in t... [Objective]Implementation of the Ten-Year Fishing Ban policy may alter fish diversity and niche characteristics of dominant species in spawning grounds within the National Nature Reserve for Rare and Endemic Fish in the Upper Yangtze River.This study initiated continuous monitoring of natural spawning habitats from February 2022 to assess these ecological changes.[Methods]Environmental DNA(eDNA)metabarcoding was employed to analyze fish species composition,biodiversity patterns,and niche parameters of dominant species.Water sampling followed the CEN/TS 19461 standard across five monitoring transects(ZT1-ZT5).[Results]The eDNA analysis detected 45 species of fish belonging to 38 genera,13 families,and 3 orders were detected through environmental DNA(eDNA)in this survey,including 10 species endemic to the upper reaches of the Yangtze River,such as Procypris rabaudi and Myxocyprinus asiaticus.The fish community was mainly composed of bottom-dwelling,settling ovum-producing,omnivorous fish.The variation ranges of the Chao1 index,ACE index,Shannon index,and Simpson index are 736~996,719~965,1.58~3.23,and 0.83~0.99,respectively,indicating that fish species in spawning sites are abundant and community distribution uniformity is high.All indexes are highest at ZT1 monitoring points.Cluster analysis showed that,at a certain similarity level,fish community types in spawning sites could be basically divided into two groups:ZT1,ZT3,and ZT5 clustered together,and ZT2 and ZT4 clustered together,indicating similar fish community habitats.There are 9 dominant fish species in typical deep pool habitats in the reserve,with niche widths(Bi)ranging from 1.13 to 3.87.The dominant fish species are broad and medium niche fish,such as Cyprinus carpio and Hemiculter tchangi,with the niche overlap index(Oik)of some dominant fish species reaching more than 0.95.This indicates fierce competition for resources among the fish in this spawning ground.[Conclusion]The Zhutuo spawning ground demonstrates high species richness with homogeneous community structure and intense resource competition.This study establishes an eDNA-based monitoring framework that enhances conventional survey method,providing critical baseline data for adaptive management under the fishing moratorium regime. 展开更多
关键词 eDNA metabarcoding fishes endemic to the upper reaches of the Yangtze River spawning ground fish diversity niche characteristics
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Arsenic speciation in more than 1600 freshwater fish samples from fifty-three waterbodies in Alberta,Canada 被引量:1
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作者 Chester Lau Xiufen Lu +8 位作者 Xiaojian Chen Karen S.Hoy Tetiana Davydiuk Jennifer A.Graydon Megan Reichert Adrienne LeBlanc Caitlyn Donadt Gian Jhangri X.Chris Le 《Journal of Environmental Sciences》 2025年第7期289-301,共13页
We report here arsenic speciation in 1643 freshwater fish samples,representing 14 common fish species from 53 waterbodies in Alberta,Canada.Arsenic species were extracted from fish muscle tissue.Arsenic species in the... We report here arsenic speciation in 1643 freshwater fish samples,representing 14 common fish species from 53 waterbodies in Alberta,Canada.Arsenic species were extracted from fish muscle tissue.Arsenic species in the extracts were separated using anion-exchange high-performance liquid chromatography(HPLC)and quantified using inductively coupled plasma mass spectrometry(ICPMS).The total arsenic concentrations in fish ranged from 2.8 to 1200μg/kg(in wet weight of sample)(mean 71±101μg/kg),which are all below the 2000μg/kg(wet weight)maximum allowable total arsenic in fish,recommended by the Ontario Ministry of the Environment.In 99.7%,or 1638 of all 1643 freshwater fish samples analyzed,arsenobetaine(AsB)was detectable,with concentrations higher than the method detection limit of 0.25μg/kg(wet weight).Dimethylarsinic acid(DMA)was detectable(concentration>0.25μg/kg)in 92.1%,or 1514 of the 1643 freshwater fish samples.Inorganic arsenate(iAs^(Ⅴ))was detectable(>0.25μg/kg)in 1119 fish(i.e.,68.1% of 1643 samples).Monomethylarsonic acid(MMA)was detectable(>0.25μg/kg)in 418 fish(25.4%of 1643 samples).The concentrations of arsenic species in the 1643 fish samples varied by as much as three orders of magnitude,ranging from below the method detection limit of 0.25μg/kg to the maximum concentrations of 380μg/kg for AsB,150μg/kg for DMA,70μg/kg for iAs^(Ⅴ),and 51μg/kg for MMA.AsB made up 46.1%±26.2% of total arsenic species.Arsenic speciation patterns varied between lake whitefish,northern pike,and walleye,the three most common types of fish analyzed.The relative proportion of DMA in northern pike was larger than in lake whitefish and walleye,and conversely,the relative proportion of iAs^(Ⅴ) was lower in northern pike.Seven unknown arsenic species were detected,and their chromatographic retention time did not match with those of available arsenic standards.At least one unknown arsenic species was detected in 33.4%,or 549 of 1643 freshwater fish samples.The concentrations of unknown arsenic species were as high as 61μg/kg.Future research is necessary to identify unknown arsenic species and to determine contributing factors to the observed arsenic species patterns and concentrations. 展开更多
关键词 Arsenic species Environmental monitoring Exposure assessment Freshwater fish Lake whitefish Northern pike WALLEYE Chromatography and mass SPECTROMETRY Speciation patterns
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Arsenic speciation in freshwater fish using high performance liquid chromatography and inductively coupled plasma mass spectrometry 被引量:1
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作者 Chester Lau Xiufen Lu +4 位作者 Karen S.Hoy Tetiana Davydiuk Jennifer A.Graydon Megan Reichert X.Chris Le 《Journal of Environmental Sciences》 2025年第7期302-315,共14页
Arsenic speciation in freshwater fish is crucial for providing meaningful consumption guidelines that allow the public to make informed decisions regarding its consumption.While marine fish have attractedmuch research... Arsenic speciation in freshwater fish is crucial for providing meaningful consumption guidelines that allow the public to make informed decisions regarding its consumption.While marine fish have attractedmuch research interest due to their higher arsenic content,research on freshwater fish is limited due to the challenges in quantifying and identifying arsenic species present at trace levels.We describe here a sensitivemethod and its application to the quantification of arsenic species in freshwater fish.Arsenic species from fish tissues were extracted using a methanol/water mixture(1:1 vol.ratio)and ultrasound sonication.Anion-exchange high-performance liquid chromatography(HPLC)enabled separation of arsenobetaine(AsB),inorganic arsenite(iAs^(Ⅲ)),dimethylarsinic acid(DMA),monomethylarsonic acid(MMA),inorganic arsenate(iAs^(Ⅴ)),and three new arsenic species.Inductively coupled plasma mass spectrometry(ICPMS)provided highly sensitive and specific detection of arsenic.A limit of detection of 0.25μg/kg(wet weight fish tissue)was achieved for the five target arsenic species:AsB,iAs^(Ⅲ),DMA,MMA,and iAs^(Ⅴ).A series of experimentswere conducted to ensure the accuracy and validity of the analytical method.The method was successfully applied to the determination of arsenic species in lakewhitefish,northern pike,and walleye,with AsB,DMA,and iAs^(Ⅴ) being frequently detected.Three new arsenic species were detected,but their chromatographic retention times did not match with those of any available arsenic standards.Future research is necessary to elucidate the identity of these new arsenic species detected in freshwater fish. 展开更多
关键词 Arsenic species Freshwater fish HPLC ICPMS Chromatography separation Inductively coupled plasma mass SPECTROMETRY Non-targeted new species Speciation method validation
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基于FISH和660K芯片的优质弱筋小麦品种内麦416的遗传构成解析
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作者 关淑仙 黄辉跃 +5 位作者 汪仁全 王相权 杨杰智 宋艳霞 黄丽萍 杨敏 《麦类作物学报》 北大核心 2025年第12期1606-1612,共7页
为探明优质弱筋小麦品种内麦416的染色体和分子遗传构成,利用荧光原位杂交(FISH)和660K芯片对内麦416及其双亲射06-245和J1094进行分析。结果表明,内麦416根尖细胞含有42条染色体,遗传稳定,4A、5A、1B和6B染色体的Oligo-pSc119.2 FISH... 为探明优质弱筋小麦品种内麦416的染色体和分子遗传构成,利用荧光原位杂交(FISH)和660K芯片对内麦416及其双亲射06-245和J1094进行分析。结果表明,内麦416根尖细胞含有42条染色体,遗传稳定,4A、5A、1B和6B染色体的Oligo-pSc119.2 FISH信号与母本射06-245有差异,除1B外,与亲本J1094也有差异。通过660K芯片结果显示,母本射06-245对内麦416的遗传贡献率为66.51%,父本J1094的遗传贡献率为33.49%,说明内麦416具有遗传偏亲现象;在基因组水平,母本射06-245对A、B和D 3个基因组的遗传贡献率(分别为73.84%、60.73%和61.64%)高于父本J1094;在染色体水平,除1B、6B、4D和7D外,其余染色体的遗传贡献率均表现为射06-245高于J1094,其中7A、3D的遗传贡献率分别高达91.98%和90.32%。经功能标记鉴定,内麦416包含6个高千粒重基因(TaGS2B1、TaGW2-6B、TaGS-D1、TaSus2-2A、TaCwi、TaGS2A1),还携带有与抗性(COMT3B、1-FEH-6B、TaDREB1、Vp1B1、Lr34)和农艺性状(Rht-D1b、TamybR_B1b)等相关的优异基因。由此可见,内麦416染色体具有遗传稳定性,表现出明显的母本遗传偏亲特征,且携带多个高千粒重基因及与抗性相关的优良基因。 展开更多
关键词 内麦416 fish 660K芯片 遗传构成
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应用HCR-FISH技术研究冷泉沉积物中甲烷厌氧氧化古菌的生存状态
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作者 何茂雨 王景 +1 位作者 李思翰 梁乐文 《海洋学研究》 北大核心 2025年第1期22-33,共12页
甲烷厌氧氧化(anaerobic oxidation of methane,AOM)过程是冷泉沉积物中元素循环过程的重要一环。该反应一般由甲烷厌氧氧化古菌(anaerobic methanotrophic archaea,ANME)和硫酸盐还原细菌(sulfate-reducing bacteria,SRB)共同完成,两... 甲烷厌氧氧化(anaerobic oxidation of methane,AOM)过程是冷泉沉积物中元素循环过程的重要一环。该反应一般由甲烷厌氧氧化古菌(anaerobic methanotrophic archaea,ANME)和硫酸盐还原细菌(sulfate-reducing bacteria,SRB)共同完成,两者通常以共生体的方式存在。然而目前尚未获得ANME纯培养菌株,且其缓慢的代谢阻碍了对其代谢特征、协同作用机制的进一步探索和研究。本文通过杂交链反应-荧光原位杂交技术(hybridization chain reaction-fluorescence in situ hybridization,HCR-FISH)并结合16S rRNA基因高通量测序结果,调查了南海Formosa冷泉(简称F冷泉)黑色菌席区域不同深度沉积物中ANME群落组成情况及存在状态。结果显示,ANME-1和ANME-2为F冷泉沉积物中的主要ANME类群,其中,ANME-2和SRB以菌团聚集体的方式存在,而未发现ANME-1类群与细菌成团的情况。这表明ANME-2多与SRB共生进行AOM过程,并且暗示ANME-1与ANME-2存在不同的生理功能和甲烷代谢机制。另外,在所有层位的沉积物样品中,ANME-2/SRB菌团直径主要集中在3~10μm之间。相关性分析表明,菌团直径分布与沉积物中硫酸盐浓度等环境因子显著相关,指示冷泉环境因子对ANME SRB菌团生长的影响作用。此外,通过HCR-FISH技术进一步发现,在F冷泉沉积物中存在多个排列整齐、大小均匀的菌团连接形成的菌团簇,这种特殊结构暗示菌团间可能存在着联系或合作关系。本研究揭示了F冷泉不同深度沉积物中ANME类群的存在状态及共生菌团的大小和分布规律,为进一步揭示不同ANME类群在原位冷泉沉积物中的甲烷代谢机制和生态功能提供了基础。 展开更多
关键词 冷泉 沉积物 ANME SRB AOM 微生物共生体 HCR-fish
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Acellular fish skin grafts in diabetic foot ulcer care:Advances and clinical insights
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作者 Yu Zhao Quan-Quan Shen 《World Journal of Diabetes》 SCIE 2025年第1期34-38,共5页
Diabetic foot ulcers(DFUs)represents a significant public health issue,with a rising global prevalence and severe potential complications including amputation.Traditional treatments often fall short due to various lim... Diabetic foot ulcers(DFUs)represents a significant public health issue,with a rising global prevalence and severe potential complications including amputation.Traditional treatments often fall short due to various limitations such as high recurrence rates and extensive resource utilization.This editorial explores the innovative use of acellular fish skin grafts as a transformative approach in DFU management.Recent studies and a detailed case report highlight the efficacy of acellular fish skin grafts in accelerating wound closure,reducing dressing changes,and enhancing patient outcomes with a lower socio-economic burden.Despite their promise,challenges such as limited availability,patient acceptance,and the need for further research persist.Addressing these through more extensive randomized controlled trials and fostering a multidisciplinary treatment approach may optimize DFU care and reduce the global health burden associated with these complex wounds. 展开更多
关键词 Diabetic foot ulcer Acellular matrix tissues Acellular fish skin grafts Wound healing Bioengineered materials
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Effects of captive-breeding conditions on metabolic and performance traits in an endangered,endemic cyprinidontiform fish
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作者 Guillem Masó Emili García-Berthou +2 位作者 Roberto Merciai Dani Latorre Anna Vila-Gispert 《Current Zoology》 2025年第1期14-24,共11页
Captive breeding and stocking are commonly employed strategies for enhancing fisheries and conserving endangered fish species.However,hatchery-raised fish often exhibit reduced performance in the wild,displaying alter... Captive breeding and stocking are commonly employed strategies for enhancing fisheries and conserving endangered fish species.However,hatchery-raised fish often exhibit reduced performance in the wild,displaying alterations in physiological,morphological,and behavioral traits.We tested for differences in swimming capacity and metabolic traits between wild and hatchery-reared individuals of the Spanish toothcarp(Aphanius iberus)from 2 different populations.Furthermore,we experimentally tested if these changes translated into fitness differences after their stocking into the wild.There were significant differences in swimming capacity and metabolic traits between wild and hatchery-reared individuals and also between the 2 populations.Captive-bred individuals displayed consistently lower metabolic rates than wild individuals from the same population(30-76% lower).Critical swimming speed rather differed between the 2 populations.Sex-specific differences were observed in maximum and standard metabolic rates,with wild individuals and females generally exhibiting higher values but with some exceptions.During a 3-month experiment,survival rates did not significantly differ between wild and captive-bred fish.Captive-bred individuals started smaller but exhibited rapid growth during the experiment.Initially,larger captive-bred fish had lower body conditions than their wild counterparts,but these differences progressively diminished.In summary,captive-bred individuals of this fish species showed lower metabolic rates,although the differences with wild individuals slightly depended on sex and size. 展开更多
关键词 fish stocking hatchery-reared fish metabolic traits protected fish species REINTRODUCTION swimming capacity
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Nutritive and Microbiological Quality of Fish Feed Formulated from Local Flours Enriched with Larvaes of Hermetia illucens
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作者 Francine Herodias Béké Nadège Kouadio-N’gbesso +4 位作者 Paul Yao Attien N’da Einstein Kouadio Audrey Charlène Asté Comoé Koffi Donatien Benie Adjehi Thomas Dadié 《Advances in Microbiology》 2025年第1期30-41,共12页
In aquaculture, feed represents the main component of production costs, and the development of this sector depends on the development of an economical feed formulation that meets the qualitative and quantitative requi... In aquaculture, feed represents the main component of production costs, and the development of this sector depends on the development of an economical feed formulation that meets the qualitative and quantitative requirements of fish. The aim of this study was to determine the nutritive and microbiological quality of fish feed formulated from local flours enriched with Hermetia illucens larvae. The raw materials used for formulation were fishmeal, corn meal, low-grade rice, soybean meal and Hermetia illucens larvae meal. Different iso-protein feed compositions were prepared with 0%, 10%, 25%, 35%, 50%, 65%, 75% and 100% incorporation of Hermetia illucens larvae meal as a substitute for fish meal. Biochemical and microbiological analyses of these flours were determined using standard methods. The results showed that incorporation of larvae meal had an influence on the biochemical characteristics ash (8.15 to 20.27%), lipid (11.55 to 24.94%), fiber (13.93 to 20.41%) and dry matter (89.65 to 91.19%) of various formulated feed. Loads of fecal Streptococci, Staphylococci, Aeromonas, yeasts and molds ranged from 2.4 to 4.9 log 10 CFU/g;3.6 to 3.9 log 10 CFU/g;2.2 to 2.7 log 10 CFU/g;2.1 to 2.3 log 10 CFU/g, respectively. The level of contamination of these flours was below the microbiological criteria applicable to animal feed. Feed formulated with 0% and 10% Hermetia illucens larvae showed the best nutritive and microbiological characteristics. These results suggest that flours enriched with Hermetia illucens larvae could be used in fish feed. 展开更多
关键词 Hermetia illucens fish Feed Nutritive and Microbiological Quality
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Dynamic Analysis and Trajectory Solution of Multi-Robot Coordinated Towing System
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作者 ZHAO Xiangtang ZHAO Zhigang +1 位作者 WEI Qizhe SU Cheng 《Journal of Shanghai Jiaotong university(Science)》 2025年第6期1134-1143,共10页
Multi-robot coordinated towing system is an under-constrained system.The dynamic response of the towing system can not be fully controlled since the rope can only provide a unidirectional constraint force to the suspe... Multi-robot coordinated towing system is an under-constrained system.The dynamic response of the towing system can not be fully controlled since the rope can only provide a unidirectional constraint force to the suspended object.Based on the kinematics of the multi-robot coordinated towing system with fixed-base,the Newton-Euler equations and Udwadia-Kalaba equations were used to establish the dynamics of the towing system.To obtain the motion trajectories with high stability and strong control,the motion trajectories of the towing system were optimized.During the towing,the transition from the relaxation state to the tension state of the rope was treated as a collision between the suspended object and the robot end.The trajectories of the towing system in terms of a single-variable and multiple-variable were solved,respectively.The simulation shows that the optimized trajectories are closer to reality and truly reflect the constraints of the ropes on the suspended object.The research results provide a basis for trajectory planning and control of the towing system. 展开更多
关键词 multi-robot system towing system dynamics rope state motion trajectory
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Cable Vector Collision Detection Algorithm for Multi-Robot Collaborative Towing System
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作者 Ll Tao ZHAO Zhigang +1 位作者 ZHU Mingtong ZHAO Xiangtang 《Journal of Shanghai Jiaotong university(Science)》 2025年第2期319-329,共11页
For the process of multi-robot collaboration to lift the same lifted object by flexible cables,the existing collision detection algorithm of cables between the environmental obstacles has the problem of misjudgment an... For the process of multi-robot collaboration to lift the same lifted object by flexible cables,the existing collision detection algorithm of cables between the environmental obstacles has the problem of misjudgment and omission.In this work,the collision detection of cable vector was studied,and the purpose of collision detection was realized by algorithm.Considering the characteristics of cables themselves,based on oriented bounding box theory,the cable optimization model and environmental obstacle model were established,and a new basic geometric collision detection model was proposed.Then a fast cable vector collision detection algorithm and an optimization principle were proposed.Finally,the rationality of the cable collision detection model and the effectiveness of the proposed algorithm were verified by simulation.Simulation results show that the proposed method can meet the requirements of the fast detection and the accuracy in complex virtual environment.The results lay a foundation for obstacle avoidance motion planning of system. 展开更多
关键词 multi-robot system bounding box CABLE-DRIVEN collision detection
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Continuous Monitoring of Multi-Robot Based on Target Point Uncertainty
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作者 Guodong Yuan Jin Xie 《Journal on Artificial Intelligence》 2025年第1期1-16,共16页
This paper addresses the problem of access efficiency in multi-robot systems to the monitoring area.A distributed algorithm for multi-robot continuous monitoring,based on the uncertainty of target points,is used to mi... This paper addresses the problem of access efficiency in multi-robot systems to the monitoring area.A distributed algorithm for multi-robot continuous monitoring,based on the uncertainty of target points,is used to minimize the uncertainty and instantaneous idle time of all target points in the task domain,while maintaining a certain access frequency to the entire task domain at regular time intervals.During monitoring,the robot uses shared information to evaluate the cumulative uncertainty and idle time of the target points,and combines the update list collected from adjacent target points with a utility function to determine the target points to be visited online.At the same time,the paper further delves into the impact of stability and scalability on multi-robot continuous monitoring algorithms in different surveillance environments.Finally,through simulation experiments and physical experiments in different environments,it has been demonstrated that the use of the algorithm presented in the paper leads to superior overall monitoring performance for robotic systems,providing assistance for research on large-scale robotic systems. 展开更多
关键词 multi-robot system UNCERTAINTY idle time continuous monitoring utility function
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A survey on passing-through control of multi-robot systems in cluttered environments
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作者 GAO Yan BAI Chenggang QUAN Quan 《Journal of Systems Engineering and Electronics》 2025年第4期1037-1056,共20页
This survey presents a comprehensive review of vari-ous methods and algorithms related to passing-through control of multi-robot systems in cluttered environments.Numerous studies have investigated this area,and we id... This survey presents a comprehensive review of vari-ous methods and algorithms related to passing-through control of multi-robot systems in cluttered environments.Numerous studies have investigated this area,and we identify several avenues for enhancing existing methods.This survey describes some models of robots and commonly considered control objec-tives,followed by an in-depth analysis of four types of algo-rithms that can be employed for passing-through control:leader-follower formation control,multi-robot trajectory planning,con-trol-based methods,and virtual tube planning and control.Fur-thermore,we conduct a comparative analysis of these tech-niques and provide some subjective and general evaluations. 展开更多
关键词 multi-robot system passing-through control forma-tion trajectory planning virtual tube.
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Load Stability Analysis of a Floating Multi-Robot Coordinated Towing System
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作者 SU Cheng ZHAO Xiangtang +2 位作者 YAN Zengzhen ZHAO Zhigang MENG Jiadong 《Journal of Shanghai Jiaotong university(Science)》 2025年第6期1162-1170,共9页
Cranes used at sea have some shortcomings in terms of flexibility,efficiency,and safety.Therefore,a floating multi-robot coordinated towing system is planned to fulfill the offshore towing requirements.It is difficult... Cranes used at sea have some shortcomings in terms of flexibility,efficiency,and safety.Therefore,a floating multi-robot coordinated towing system is planned to fulfill the offshore towing requirements.It is difficult to study the stability of a floating multi-robot coordinated towing system by ancient strategies.First,the minimum tension of the rope and the minimum singular value of the stiffness matrix were separately used to analyze the load stability.The advantages and disadvantages of the two methods were discussed.Then,the two stability analysis methods were normalized and weighted to obtain the method based on minimum tension and minimum singular to comprehensively analyze the stability of the load.Finally,the effect of different weighting coefficients on the load stability was analyzed,which led to a reasonable weighting coefficient to evaluate the load stability by comparing with a single analysis method.The research results provide a basis for the motion planning and coordinated control of the towing system. 展开更多
关键词 offshore towing multi-robot system load stability minimum tension minimum singular
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