The Financial Technology(FinTech)sector has witnessed rapid growth,resulting in increasingly complex and high-volume digital transactions.Although this expansion improves efficiency and accessibility,it also introduce...The Financial Technology(FinTech)sector has witnessed rapid growth,resulting in increasingly complex and high-volume digital transactions.Although this expansion improves efficiency and accessibility,it also introduces significant vulnerabilities,including fraud,money laundering,and market manipulation.Traditional anomaly detection techniques often fail to capture the relational and dynamic characteristics of financial data.Graph Neural Networks(GNNs),capable of modeling intricate interdependencies among entities,have emerged as a powerful framework for detecting subtle and sophisticated anomalies.However,the high-dimensionality and inherent noise of FinTech datasets demand robust feature selection strategies to improve model scalability,performance,and interpretability.This paper presents a comprehensive survey of GNN-based approaches for anomaly detection in FinTech,with an emphasis on the synergistic role of feature selection.We examine the theoretical foundations of GNNs,review state-of-the-art feature selection techniques,analyze their integration with GNNs,and categorize prevalent anomaly types in FinTech applications.In addition,we discuss practical implementation challenges,highlight representative case studies,and propose future research directions to advance the field of graph-based anomaly detection in financial systems.展开更多
Background Frailty is common and significantly impacts prognosis in heart failure(HF). The Vulnerable Elders Survey-13(VES-13), widely used in oncogeriatrics and public health, remains unexplored as a frailty screenin...Background Frailty is common and significantly impacts prognosis in heart failure(HF). The Vulnerable Elders Survey-13(VES-13), widely used in oncogeriatrics and public health, remains unexplored as a frailty screening tool in HF outpatients. In this study, we prospectively evaluated VES-13 against a multimodal screening assessment in detecting frailty and predicting individual risk of adverse prognosis.Methods Frailty was assessed at the initial visit using both a multimodal approach, incorporating Barthel Index, Older American Resources and Services scale, Pfeiffer Test, abbreviated Geriatric Depression Scale, age > 85 years, lacking support systems,and VES-13. Patients scoring ≥ 3 on VES-13 or meeting at least one multimodal criterion were classified as frail. Endpoints included all-cause mortality, a composite of death or HF hospitalization, and recurrent HF hospitalizations.Results A total of 301 patients were evaluated. VES-13 identified 40.2% as frail and the multimodal assessment 33.2%. In Cox regression analyses, frailty identified by VES-13 showed greater prognostic significance than the multimodal assessment for allcause mortality(HR = 3.70 [2.15–6.33], P < 0.001 vs. 2.40 [1.46–4.0], P = 0.001) and the composite endpoint(HR = 3.13 [2.02–4.84], P< 0.001 vs. 1.96 [1.28–2.99], P = 0.002). Recurrent HF hospitalizations were four times more frequent in VES-13 frail patients while two times in those identified as frail by the multimodal assessment. Additionally, stratifying patients by VES-13 tertiles provided robust risk differentiation.Conclusions VES-13, a simple frailty tool, outperformed a comprehensive multimodal assessment and could be easily integrated into routine HF care, highlighting its clinical utility in identifying patients at risk for poor outcomes.展开更多
INTRODUCTION.On May 1st,2024,around 2:10 a.m.,a catastrophic collapse occurred along the Meilong Expressway near Meizhou City,Guangdong Province,China,at coordinates 24°29′24″N and 116°40′25″E.This colla...INTRODUCTION.On May 1st,2024,around 2:10 a.m.,a catastrophic collapse occurred along the Meilong Expressway near Meizhou City,Guangdong Province,China,at coordinates 24°29′24″N and 116°40′25″E.This collapse resulted in a pavement failure of approximately 17.9 m in length and covering an area of about 184.3 m^(2)(Chinanews,2024).展开更多
Detection of ore waste is crucial for achieving automation in mineral metallurgy production.However,deep learning-based target detection algorithms still face several challenges in iron waste screening,including poor ...Detection of ore waste is crucial for achieving automation in mineral metallurgy production.However,deep learning-based target detection algorithms still face several challenges in iron waste screening,including poor lighting conditions in underground mining environments,dust disturbances,platform vibrations during operation,and limited resources for large-scale computing equipment.These factors contribute to extended computation times and unsatisfactory detection accuracy.To address these challenges,this paper proposes an ore waste detection algorithm based on an improved version of YOLOv5.To enhance feature extraction capabilities,the RepLKNet module is incorporated into the YOLOv5 backbone and neck networks.This module enhances the deformation information of feature extraction with the maximum effective Receptive Field to increase the model's accuracy.The Normalizationbased Attention Module(NAM)was introduced to enhance the attention mechanism by focusing on the most relevant features.This improves accuracy in detecting objects against noisy or unclear backgrounds,thereby further enhancing detection performance while reducing model parameters.Additionally,the loss function is optimized to constrain angular deviation using the SIOU loss function,which prevents the training frame from drifting during training and enhances convergence speed.To validate the performance of the proposed method,we tested it using a self-constructed dataset comprising 1,328 images obtained from the crushing station at Jinchuan Group's No.2 mine.The results indicate that,compared to YOLOv5s on the self-constructed dataset,the proposed algorithm achieves an 18.3%improvement in mAP(0.5),a 54%reduction in FLOPs,and a 52.53%decrease in model parameters.The effectiveness and superiority of the proposed algorithm are demonstrated through case studies and comparative analyses.展开更多
Tomato plant diseases often first manifest on the leaves,making the detection of tomato leaf diseases particularly crucial for the tomato cultivation industry.However,conventional deep learning models face challenges ...Tomato plant diseases often first manifest on the leaves,making the detection of tomato leaf diseases particularly crucial for the tomato cultivation industry.However,conventional deep learning models face challenges such as large model sizes and slow detection speeds when deployed on resource-constrained platforms and agricultural machinery.This paper proposes a lightweight model for detecting tomato leaf diseases,named LT-YOLO,based on the YOLOv8n architecture.First,we enhance the C2f module into a RepViT Block(RVB)with decoupled token and channel mixers to reduce the cost of feature extraction.Next,we incorporate a novel Efficient Multi-Scale Attention(EMA)mechanism in the deeper layers of the backbone to improve detection of critical disease features.Additionally,we design a lightweight detection head,LT-Detect,using Partial Convolution(PConv)to significantly reduce the classification and localization costs during detection.Finally,we introduce a Receptive Field Block(RFB)in the shallow layers of the backbone to expand the model’s receptive field,enabling effective detection of diseases at various scales.The improved model reduces the number of parameters by 43%and the computational load by 50%.Additionally,it achieves a mean Average Precision(mAP)of 90.9%on a publicly available dataset containing 3641 images of tomato leaf diseases,with only a 0.7%decrease compared to the baseline model.This demonstrates that the model maintains excellent accuracy while being lightweight,making it suitable for rapid detection of tomato leaf diseases.展开更多
Listeria monocytogenes(LM)is a dangerous foodborne pathogen for humans.One emerging and validated method of indirectly assessing LM in food is detecting 3-hydroxy-2-butanone(3H2B)gas.In this study,the synthesis of 3-(...Listeria monocytogenes(LM)is a dangerous foodborne pathogen for humans.One emerging and validated method of indirectly assessing LM in food is detecting 3-hydroxy-2-butanone(3H2B)gas.In this study,the synthesis of 3-(2-aminoethylamino)propyltrimethoxysilane(AAPTMS)functionalized hierarchical hollow TiO_(2)nanospheres was achieved via precise controlling of solvothermal reaction temperature and post-grafting route.The sensors based on as-prepared materials exhibited excellent sensitivity(480 Hz@50 ppm),low detection limit(100 ppb),and outstanding selectivity.Moreover,the evaluation of LM with high sensitivity and specificity was achieved using the sensors.Such stable three-dimensional spheres,whose distinctive hierarchical and hollow nanostructure simultaneously improved both sensitivity and response/recovery speed dramatically,were spontaneously assembled by nanosheets.Meanwhile,the moderate loadings of AAPTMS significantly improved the selectivity of sensors.Then,the gas-sensing mechanism was explored by utilizing thermodynamic investigation,Gaussian 16 software,and in situ diffuse reflectance infrared transform spectroscopy,illustrating the weak chemisorption between the-NHgroup and 3H2B molecules.These portable sensors are promising for real-time assessment of LM at room temperature,which will make a magnificent contribution to food safety.展开更多
In this article,the multi-parameters Mittag-Leffler function is studied in detail.As a consequence,a series of novel results such as the integral representation,series representation and Mellin transform to the above ...In this article,the multi-parameters Mittag-Leffler function is studied in detail.As a consequence,a series of novel results such as the integral representation,series representation and Mellin transform to the above function,are obtained.Especially,we associate the multi-parameters Mittag-Leffler function with two special functions which are the generalized Wright hypergeometric and the Fox’s-H functions.Meanwhile,some interesting integral operators and derivative operators of this function,are also discussed.展开更多
Small-drone technology has opened a range of new applications for aerial transportation. These drones leverage the Internet of Things (IoT) to offer cross-location services for navigation. However, they are susceptibl...Small-drone technology has opened a range of new applications for aerial transportation. These drones leverage the Internet of Things (IoT) to offer cross-location services for navigation. However, they are susceptible to security and privacy threats due to hardware and architectural issues. Although small drones hold promise for expansion in both civil and defense sectors, they have safety, security, and privacy threats. Addressing these challenges is crucial to maintaining the security and uninterrupted operations of these drones. In this regard, this study investigates security, and preservation concerning both the drones and Internet of Drones (IoD), emphasizing the significance of creating drone networks that are secure and can robustly withstand interceptions and intrusions. The proposed framework incorporates a weighted voting ensemble model comprising three convolutional neural network (CNN) models to enhance intrusion detection within the network. The employed CNNs are customized 1D models optimized to obtain better performance. The output from these CNNs is voted using a weighted criterion using a 0.4, 0.3, and 0.3 ratio for three CNNs, respectively. Experiments involve using multiple benchmark datasets, achieving an impressive accuracy of up to 99.89% on drone data. The proposed model shows promising results concerning precision, recall, and F1 as indicated by their obtained values of 99.92%, 99.98%, and 99.97%, respectively. Furthermore, cross-validation and performance comparison with existing works is also carried out. Findings indicate that the proposed approach offers a prospective solution for detecting security threats for aerial systems and satellite systems with high accuracy.展开更多
Under sensorless control, the position estimation error in interior permanent magnet(PM) synchronous machines will lead to parameter identification errors and a rank-deficiency issue. This paper proposes a parameter i...Under sensorless control, the position estimation error in interior permanent magnet(PM) synchronous machines will lead to parameter identification errors and a rank-deficiency issue. This paper proposes a parameter identification model that is independent of position error by combining the dq-axis voltage equations. Then, a novel dual signal alternate injection method is proposed to address the rank-deficiency issue, i.e., during one injection period, a zero, positive, and negative d-axis current injection together with a rotor position offset injection, to simultaneously identify the multi-parameters, including stator resistance, dq-axis inductances, and PM flux linkage. The proposed method is verified by experiments at different dq-axis current conditions.展开更多
BACKGROUND Diagnosing bacterial infections(BI)in patients with cirrhosis can be challenging because of unclear symptoms,low diagnostic accuracy,and lengthy culture testing times.Various biomarkers have been studied,in...BACKGROUND Diagnosing bacterial infections(BI)in patients with cirrhosis can be challenging because of unclear symptoms,low diagnostic accuracy,and lengthy culture testing times.Various biomarkers have been studied,including serum procal-citonin(PCT)and presepsin.However,the diagnostic performance of these markers remains unclear,requiring further informative studies to ascertain their diagnostic value.AIM To evaluate the pooled diagnostic performance of PCT and presepsin in detecting BI among patients with cirrhosis.INTRODUCTION Bacterial infections(BI)commonly occur in patients with cirrhosis,resulting in poor outcomes,including the development of cirrhotic complications,septic shock,acute-on-chronic liver failure(ACLF),multiple organ failures,and mortality[1,2].BI is observed in 20%-30%of hospitalized patients,with and without ACLF[3].Patients with cirrhosis are susceptible to BI because of internal and external factors.The major internal factors are changes in gut microbial composition and function,bacterial translocation,and cirrhosis-associated immune dysfunction syndrome[4,5].External factors include alcohol use,proton-pump inhibitor use,frailty,readmission,and invasive procedures.Spontaneous bacterial peritonitis(SBP),urinary tract infection,pneumonia,and primary bacteremia are the common BIs in hospit-alized patients with cirrhosis[6].Early diagnosis and adequate empirical antibiotic therapy are two critical factors that improve the prognosis of BI in patients with cirrhosis.However,early detection of BI in cirrhosis is challenging due to subtle clinical signs and symptoms,low sensitivity and specificity of systemic inflammatory response syndrome criteria,and low sensitivity of bacterial cultures.Thus,effective biomarkers need to be identified for the early detection of BI.Several biomarkers have been evaluated,but their efficacy in detecting BI is unclear.Procalcitonin(PCT)is a precursor of the hormone calcitonin,which is secreted by parafollicular cells of the thyroid gland[7].In the presence of BI,PCT gene expression increases in extrathyroidal tissues,causing a subsequent increase in serum PCT level[8].Changes in serum PCT are detectable as early as 4 hours after infection onset and peaks between 8 and 24 hours,making it a valuable diagnostic biomarker for BI.Several studies have demonstrated the favorable diagnostic accuracy of PCT in the diagnosis of BI in individuals with cirrhosis[9-13]and without cirrhosis[14-16].Since 2014,two meta-analyses have been published on the diagnostic value of PCT for SBP and BI in patients with cirrhosis[17,18].Other related studies have been conducted since then[10-12,19-33].Serum presepsin has recently emerged as a promising biomarker for diagnosing BI.This biomarker is the N-terminal fraction protein of the soluble CD14 g-negative bacterial lipopolysaccharide–lipopolysaccharide binding protein(sCD14-LPS-LBP)complex,which is cleaved by inflammatory serum protease in response to BI[34].Presepsin levels increase within 2 hours and peaks in 3 hours[35].This is useful for detecting BI since presepsin levels increase earlier than serum Our systematic review and meta-analysis was performed with adherence to PRISMA guidelines[37].展开更多
Depression is increasingly prevalent among adolescents and can profoundly impact their lives.However,the early detection of depression is often hindered by the timeconsuming diagnostic process and the absence of objec...Depression is increasingly prevalent among adolescents and can profoundly impact their lives.However,the early detection of depression is often hindered by the timeconsuming diagnostic process and the absence of objective biomarkers.In this study,we propose a novel approach for depression detection based on an affective brain-computer interface(aBCI)and the resting-state electroencephalogram(EEG).By fusing EEG features associated with both emotional and resting states,our method captures comprehensive depression-related information.The final depression detection model,derived through decision fusion with multiple independent models,further enhances detection efficacy.Our experiments involved 40 adolescents with depression and 40 matched controls.The proposed model achieved an accuracy of 86.54%on cross-validation and 88.20%on the independent test set,demonstrating the efficiency of multi-modal fusion.In addition,further analysis revealed distinct brain activity patterns between the two groups across different modalities.These findings hold promise for new directions in depression detection and intervention.展开更多
Visible and infrared(RGB-IR)fusion object detection plays an important role in security,disaster relief,etc.In recent years,deep-learning-based RGB-IR fusion detection methods have been developing rapidly,but still st...Visible and infrared(RGB-IR)fusion object detection plays an important role in security,disaster relief,etc.In recent years,deep-learning-based RGB-IR fusion detection methods have been developing rapidly,but still struggle to deal with the complex and changing scenarios captured by drones,mainly due to two reasons:(A)RGB-IR fusion detectors are susceptible to inferior inputs that degrade performance and stability.(B)RGB-IR fusion detectors are susceptible to redundant features that reduce accuracy and efficiency.In this paper,an innovative RGB-IR fusion detection framework based on global-local feature optimization,named GLFDet,is proposed to improve the detection performance and efficiency of drone-captured objects.The key components of GLFDet include a Global Feature Optimization(GFO)module,a Local Feature Optimization(LFO)module and a Channel Separation Fusion(CSF)module.Specifically,GFO calculates the information content of the input image from the frequency domain and optimizes the features holistically.Then,LFO dynamically selects high-value features and filters out low-value features before fusion,which significantly improves the efficiency of fusion.Finally,CSF fuses the RGB and IR features across the corresponding channels,which avoids the rearrangement of the channel relationships and enhances the model stability.Extensive experimental results show that the proposed method achieves the best performance on three popular RGB-IR datasets Drone Vehicle,VEDAI,and LLVIP.In addition,GLFDet is more lightweight than other comparable models,making it more appealing to edge devices such as drones.The code is available at https://github.com/lao chen330/GLFDet.展开更多
Driver distraction is a leading cause of traffic accidents,with fatigue being a significant contributor.This paper introduces a novel method for detecting driver distraction by analyzing facial features using machine ...Driver distraction is a leading cause of traffic accidents,with fatigue being a significant contributor.This paper introduces a novel method for detecting driver distraction by analyzing facial features using machine deep learning and 68 face model.The proposed system assesses driver tiredness by measuring the distance between key facial landmarks,such as the distance between the eyes and the angle of the mouth,to evaluate signs of drowsiness or disengagement.Real-time video feed analysis allows for continuous monitoring of the driver’s face,enabling the system to detect behavioral cues associated with distraction,such as eye closures or changes in facial expressions.The effectiveness of this method is demonstrated through a series of experiments on a dataset of driver videos,which proves that the approach can accurately assess tiredness and distraction levels under various driving conditions.By focusing on facial landmarks,the system is computationally efficient and capable of operating in real-time,making it a practical solution for in-vehicle safety systems.This paper discusses the system’s performance,limitations,and potential for future enhancements,including integration with other in-vehicle technologies to provide comprehensive driver monitoring.展开更多
Detection of Autism Spectrum Disorder(ASD)is a crucial area of research,representing a foundational aspect of psychological studies.The advancement of technology and the widespread adoption of machine learning methodo...Detection of Autism Spectrum Disorder(ASD)is a crucial area of research,representing a foundational aspect of psychological studies.The advancement of technology and the widespread adoption of machine learning methodologies have brought significant attention to this field in recent years.Interdisciplinary efforts have further propelled research into detection methods.Consequently,this study aims to contribute to both the fields of psychology and computer science.Specifically,the goal is to apply machine learning techniques to limited data for the detection of Autism Spectrum Disorder.This study is structured into two distinct phases:data preprocessing and classification.In the data preprocessing phase,four datasets—Toddler,Children,Adolescent,and Adult—were converted into numerical form,adjusted as necessary,and subsequently clustered.Clustering was performed using six different methods:Kmeans,agglomerative,DBSCAN(Density-Based Spatial Clustering of Applications with Noise),mean shift,spectral,and Birch.In the second phase,the clustered ASD data were classified.The model’s accuracy was assessed using 5-fold cross-validation to ensure robust evaluation.In total,ten distinct machine learning algorithms were employed.The findings indicate that all clustering methods demonstrated success with various classifiers.Notably,the K-means algorithm emerged as particularly effective,achieving consistent and significant results across all datasets.This study is expected to serve as a guide for improving ASD detection performance,even with minimal data availability.展开更多
Strong-field terahertz(THz) radiation holds significant potential in non-equilibrium state manipulation, electron acceleration, and biomedical effects. However, distortion-free detection of strong-field THz waveforms ...Strong-field terahertz(THz) radiation holds significant potential in non-equilibrium state manipulation, electron acceleration, and biomedical effects. However, distortion-free detection of strong-field THz waveforms remains an essential challenge in THz science and technology. To address this issue, we propose a ferromagnetic detection scheme based on Zeeman torque sampling, achieving distortion-free strong-field THz waveform detection in Py films. Thickness-dependent characterization(3–21 nm) identifies peak detection performance at 21 nm within the investigated range. Furthermore, by structurally engineering the Py ferromagnetic layer, we demonstrate strong-field THz detection in symmetric Ta(3 nm)/Py(9 nm)/Ta(3 nm) heterostructure while simultaneously resolving Zeeman torque responses and collective spin-wave dynamics in asymmetric W(4 nm)/Py(9 nm)/Pt(2 nm)heterostructure. We calculated spin wave excitations and spin orbit torque distributions in asymmetric heterostructures, along with spin wave excitations in symmetric modes. This approach overcomes the sensitivity limitations of conventional techniques in strong-field conditions.展开更多
Anomaly detection is an important research area in a diverse range of real-world applications.Although many algorithms have been proposed to address anomaly detection for numerical datasets,categorical and mixed datas...Anomaly detection is an important research area in a diverse range of real-world applications.Although many algorithms have been proposed to address anomaly detection for numerical datasets,categorical and mixed datasets remain a significant challenge,primarily because a natural distance metric is lacking.Consequently,the methods proposed in the literature implement entirely different assumptions regarding the definition of cate-gorical anomalies.This paper presents a novel categorical anomaly detection approach,offering two key con-tributions to existing methods.First,a novel surprisal-based anomaly score is introduced,which provides a more accurate assessment of anomalies by considering the full distribution of categorical values.Second,the proposed method considers complex correlations in the data beyond the pairwise interactions of features.This study proposed and tested the novel categorical surprisal anomaly detection algorithm(CSAD)by comparing and evaluating it against six competitors.The experimental results indicate that CSAD produced the best overall performance,achieving the highest average ROC-AUC and PR-AUC values of 0.8 and 0.443,respectively.Furthermore,CSAD's execution time is satisfactory even when processing large,high-dimensional datasets.展开更多
At an age when most teens are figuring out high school,Siddharth is already shaping the future of medical tech.The 14⁃year⁃old boy from Dallas has created an AI⁃powered app,Circadian AI,capable of detecting heart dise...At an age when most teens are figuring out high school,Siddharth is already shaping the future of medical tech.The 14⁃year⁃old boy from Dallas has created an AI⁃powered app,Circadian AI,capable of detecting heart disease in just 7 seconds using only a smartphone's microphone.展开更多
Non-cooperative communication detection is a key technology for locating radio interfer-ence sources and conducting reconnaissance on adversary radiation sources.To meet the require-ments of wide-area monitoring,a sin...Non-cooperative communication detection is a key technology for locating radio interfer-ence sources and conducting reconnaissance on adversary radiation sources.To meet the require-ments of wide-area monitoring,a single interception channel often contains mixed multi-source sig-nals and interference,resulting in generally low signal-to-noise ratio(SNR)of the received signals;meanwhile,improving detection quality urgently requires either high frequency resolution or high-time resolution,which poses severe challenges to detection techniques based on time-frequency rep-resentations(TFR).To address this issue,this paper proposes a fixed-frame-structure signal detec-tion algorithm that integrates image enhancement and multi-scale template matching:first,the Otsu-Sauvola hybrid thresholding algorithm is employed to enhance TFR features,suppress noise interference,and extract time-frequency parameters of potential target signals(such as bandwidth and occurrence time);then,by exploiting the inherent time-frequency characteristics of the fixed-frame structure,the signal is subjected to multi-scale transformation(with either high-frequency resolution or high-time resolution),and accurate detection is achieved through the corresponding multi-scale template matching.Experimental results demonstrate that under 0 dB SNR conditions,the proposed algorithm achieves a detection rate greater than 87%,representing a significant improvement over traditional methods.展开更多
Nanochannel technology based on ionic current rectification has emerged as a powerful tool for the detection of biomolecules owing to unique advantages.Nevertheless,existing nanochannel sensors mainly focus on the det...Nanochannel technology based on ionic current rectification has emerged as a powerful tool for the detection of biomolecules owing to unique advantages.Nevertheless,existing nanochannel sensors mainly focus on the detection of targets in solution or inside the cells,moreover,they only have a single function,greatly limiting their application.Herein,we fabricated SuperDNA self-assembled conical nanochannel,which was clamped in the middle of self-made device for two functions:Online detecting living cells released TNF-αand studying intercellular communication.Polyethylene terephthalate(PET)membrane incubated tumor associated macrophages and tumor cells was rolled up and inserted into the left and right chamber of the device,respectively.Through monitoring the ion current change in the nanochannel,tumor associated macrophages released TNF-αcould be in situ and noninvasive detected with a detection limit of 0.23 pg/mL.Furthermore,the secreted TNF-αinduced epithelial-mesenchymal transformation of tumor cells in the right chamber was also studied.The presented strategy displayed outstanding performance and multi-function,providing a promising platform for in situ non-destructive detection of cell secretions and related intercellular communication analysis.展开更多
The continuous mutation and rapid spread of the severe acute respiratory syndrome coronavirus 2(SARSCoV-2)have led to the ineffectiveness of many antiviral drugs targeting the original strain.To keep pace with the vi...The continuous mutation and rapid spread of the severe acute respiratory syndrome coronavirus 2(SARSCoV-2)have led to the ineffectiveness of many antiviral drugs targeting the original strain.To keep pace with the virus’evolutionary speed,there is a crucial need for the development of rapid,cost-effective,and efficient inhibitor screening methods.In this study,we created a novel approach based on fluorescence resonance energy transfer(FRET)technology for in vitro detection of inhibitors targeting the interaction between the SARS-CoV-2 spike protein RBD(s-RBD)and the virus receptor angiotensin-converting enzyme 2(ACE2).Utilizing crystallographic insights into the s-RBD/ACE2 interaction,we modified ACE2 by fusing SNAP tag to its N-terminus(resulting in SA740)and Halo tag to s-RBD’s C-terminus(producing R525H and R541H),thereby ensuring the proximity(<10 nm)of labeled FRET dyes.We found that relative to the R541H fusion protein,R525H exhibited higher FRET efficiency,which attributed to the shortened distance between FRET dyes due to the truncation of s-RBD.Utilizing the sensitive FRET effect between SA740 and R525H,we evaluated its efficacy in detecting inhibitors of SARS-CoV-2 entry in solution and live cells.Ultimately,this FRET-based detection method was demonstrated high sensitivity,rapidity,and simplicity in solution and held promise for high-throughput screening of SARS-CoV-2 inhibitors.展开更多
基金supported by Ho Chi Minh City Open University,Vietnam under grant number E2024.02.1CD and Suan Sunandha Rajabhat University,Thailand.
文摘The Financial Technology(FinTech)sector has witnessed rapid growth,resulting in increasingly complex and high-volume digital transactions.Although this expansion improves efficiency and accessibility,it also introduces significant vulnerabilities,including fraud,money laundering,and market manipulation.Traditional anomaly detection techniques often fail to capture the relational and dynamic characteristics of financial data.Graph Neural Networks(GNNs),capable of modeling intricate interdependencies among entities,have emerged as a powerful framework for detecting subtle and sophisticated anomalies.However,the high-dimensionality and inherent noise of FinTech datasets demand robust feature selection strategies to improve model scalability,performance,and interpretability.This paper presents a comprehensive survey of GNN-based approaches for anomaly detection in FinTech,with an emphasis on the synergistic role of feature selection.We examine the theoretical foundations of GNNs,review state-of-the-art feature selection techniques,analyze their integration with GNNs,and categorize prevalent anomaly types in FinTech applications.In addition,we discuss practical implementation challenges,highlight representative case studies,and propose future research directions to advance the field of graph-based anomaly detection in financial systems.
文摘Background Frailty is common and significantly impacts prognosis in heart failure(HF). The Vulnerable Elders Survey-13(VES-13), widely used in oncogeriatrics and public health, remains unexplored as a frailty screening tool in HF outpatients. In this study, we prospectively evaluated VES-13 against a multimodal screening assessment in detecting frailty and predicting individual risk of adverse prognosis.Methods Frailty was assessed at the initial visit using both a multimodal approach, incorporating Barthel Index, Older American Resources and Services scale, Pfeiffer Test, abbreviated Geriatric Depression Scale, age > 85 years, lacking support systems,and VES-13. Patients scoring ≥ 3 on VES-13 or meeting at least one multimodal criterion were classified as frail. Endpoints included all-cause mortality, a composite of death or HF hospitalization, and recurrent HF hospitalizations.Results A total of 301 patients were evaluated. VES-13 identified 40.2% as frail and the multimodal assessment 33.2%. In Cox regression analyses, frailty identified by VES-13 showed greater prognostic significance than the multimodal assessment for allcause mortality(HR = 3.70 [2.15–6.33], P < 0.001 vs. 2.40 [1.46–4.0], P = 0.001) and the composite endpoint(HR = 3.13 [2.02–4.84], P< 0.001 vs. 1.96 [1.28–2.99], P = 0.002). Recurrent HF hospitalizations were four times more frequent in VES-13 frail patients while two times in those identified as frail by the multimodal assessment. Additionally, stratifying patients by VES-13 tertiles provided robust risk differentiation.Conclusions VES-13, a simple frailty tool, outperformed a comprehensive multimodal assessment and could be easily integrated into routine HF care, highlighting its clinical utility in identifying patients at risk for poor outcomes.
基金supported by the National Natural Science Foundation of China(Nos.42371094,41907253)partially supported by the Interdisciplinary Cultivation Program of Xidian University(No.21103240005)the Postdoctoral Fellowship Program of CPSF(No.GZB20240589)。
文摘INTRODUCTION.On May 1st,2024,around 2:10 a.m.,a catastrophic collapse occurred along the Meilong Expressway near Meizhou City,Guangdong Province,China,at coordinates 24°29′24″N and 116°40′25″E.This collapse resulted in a pavement failure of approximately 17.9 m in length and covering an area of about 184.3 m^(2)(Chinanews,2024).
基金supported by the Department of science and technology of Shaanxi Province(NO.2023-ZDLGY-24).
文摘Detection of ore waste is crucial for achieving automation in mineral metallurgy production.However,deep learning-based target detection algorithms still face several challenges in iron waste screening,including poor lighting conditions in underground mining environments,dust disturbances,platform vibrations during operation,and limited resources for large-scale computing equipment.These factors contribute to extended computation times and unsatisfactory detection accuracy.To address these challenges,this paper proposes an ore waste detection algorithm based on an improved version of YOLOv5.To enhance feature extraction capabilities,the RepLKNet module is incorporated into the YOLOv5 backbone and neck networks.This module enhances the deformation information of feature extraction with the maximum effective Receptive Field to increase the model's accuracy.The Normalizationbased Attention Module(NAM)was introduced to enhance the attention mechanism by focusing on the most relevant features.This improves accuracy in detecting objects against noisy or unclear backgrounds,thereby further enhancing detection performance while reducing model parameters.Additionally,the loss function is optimized to constrain angular deviation using the SIOU loss function,which prevents the training frame from drifting during training and enhances convergence speed.To validate the performance of the proposed method,we tested it using a self-constructed dataset comprising 1,328 images obtained from the crushing station at Jinchuan Group's No.2 mine.The results indicate that,compared to YOLOv5s on the self-constructed dataset,the proposed algorithm achieves an 18.3%improvement in mAP(0.5),a 54%reduction in FLOPs,and a 52.53%decrease in model parameters.The effectiveness and superiority of the proposed algorithm are demonstrated through case studies and comparative analyses.
文摘Tomato plant diseases often first manifest on the leaves,making the detection of tomato leaf diseases particularly crucial for the tomato cultivation industry.However,conventional deep learning models face challenges such as large model sizes and slow detection speeds when deployed on resource-constrained platforms and agricultural machinery.This paper proposes a lightweight model for detecting tomato leaf diseases,named LT-YOLO,based on the YOLOv8n architecture.First,we enhance the C2f module into a RepViT Block(RVB)with decoupled token and channel mixers to reduce the cost of feature extraction.Next,we incorporate a novel Efficient Multi-Scale Attention(EMA)mechanism in the deeper layers of the backbone to improve detection of critical disease features.Additionally,we design a lightweight detection head,LT-Detect,using Partial Convolution(PConv)to significantly reduce the classification and localization costs during detection.Finally,we introduce a Receptive Field Block(RFB)in the shallow layers of the backbone to expand the model’s receptive field,enabling effective detection of diseases at various scales.The improved model reduces the number of parameters by 43%and the computational load by 50%.Additionally,it achieves a mean Average Precision(mAP)of 90.9%on a publicly available dataset containing 3641 images of tomato leaf diseases,with only a 0.7%decrease compared to the baseline model.This demonstrates that the model maintains excellent accuracy while being lightweight,making it suitable for rapid detection of tomato leaf diseases.
基金supported by the National Natural Science Foundation of China(No.32272399)the Shanghai Natural Science Foundation(No.21ZR1427500).
文摘Listeria monocytogenes(LM)is a dangerous foodborne pathogen for humans.One emerging and validated method of indirectly assessing LM in food is detecting 3-hydroxy-2-butanone(3H2B)gas.In this study,the synthesis of 3-(2-aminoethylamino)propyltrimethoxysilane(AAPTMS)functionalized hierarchical hollow TiO_(2)nanospheres was achieved via precise controlling of solvothermal reaction temperature and post-grafting route.The sensors based on as-prepared materials exhibited excellent sensitivity(480 Hz@50 ppm),low detection limit(100 ppb),and outstanding selectivity.Moreover,the evaluation of LM with high sensitivity and specificity was achieved using the sensors.Such stable three-dimensional spheres,whose distinctive hierarchical and hollow nanostructure simultaneously improved both sensitivity and response/recovery speed dramatically,were spontaneously assembled by nanosheets.Meanwhile,the moderate loadings of AAPTMS significantly improved the selectivity of sensors.Then,the gas-sensing mechanism was explored by utilizing thermodynamic investigation,Gaussian 16 software,and in situ diffuse reflectance infrared transform spectroscopy,illustrating the weak chemisorption between the-NHgroup and 3H2B molecules.These portable sensors are promising for real-time assessment of LM at room temperature,which will make a magnificent contribution to food safety.
基金Supported by The National Undergraduate Innovation Training Program(Grant No.202310290069Z).
文摘In this article,the multi-parameters Mittag-Leffler function is studied in detail.As a consequence,a series of novel results such as the integral representation,series representation and Mellin transform to the above function,are obtained.Especially,we associate the multi-parameters Mittag-Leffler function with two special functions which are the generalized Wright hypergeometric and the Fox’s-H functions.Meanwhile,some interesting integral operators and derivative operators of this function,are also discussed.
文摘Small-drone technology has opened a range of new applications for aerial transportation. These drones leverage the Internet of Things (IoT) to offer cross-location services for navigation. However, they are susceptible to security and privacy threats due to hardware and architectural issues. Although small drones hold promise for expansion in both civil and defense sectors, they have safety, security, and privacy threats. Addressing these challenges is crucial to maintaining the security and uninterrupted operations of these drones. In this regard, this study investigates security, and preservation concerning both the drones and Internet of Drones (IoD), emphasizing the significance of creating drone networks that are secure and can robustly withstand interceptions and intrusions. The proposed framework incorporates a weighted voting ensemble model comprising three convolutional neural network (CNN) models to enhance intrusion detection within the network. The employed CNNs are customized 1D models optimized to obtain better performance. The output from these CNNs is voted using a weighted criterion using a 0.4, 0.3, and 0.3 ratio for three CNNs, respectively. Experiments involve using multiple benchmark datasets, achieving an impressive accuracy of up to 99.89% on drone data. The proposed model shows promising results concerning precision, recall, and F1 as indicated by their obtained values of 99.92%, 99.98%, and 99.97%, respectively. Furthermore, cross-validation and performance comparison with existing works is also carried out. Findings indicate that the proposed approach offers a prospective solution for detecting security threats for aerial systems and satellite systems with high accuracy.
文摘Under sensorless control, the position estimation error in interior permanent magnet(PM) synchronous machines will lead to parameter identification errors and a rank-deficiency issue. This paper proposes a parameter identification model that is independent of position error by combining the dq-axis voltage equations. Then, a novel dual signal alternate injection method is proposed to address the rank-deficiency issue, i.e., during one injection period, a zero, positive, and negative d-axis current injection together with a rotor position offset injection, to simultaneously identify the multi-parameters, including stator resistance, dq-axis inductances, and PM flux linkage. The proposed method is verified by experiments at different dq-axis current conditions.
文摘BACKGROUND Diagnosing bacterial infections(BI)in patients with cirrhosis can be challenging because of unclear symptoms,low diagnostic accuracy,and lengthy culture testing times.Various biomarkers have been studied,including serum procal-citonin(PCT)and presepsin.However,the diagnostic performance of these markers remains unclear,requiring further informative studies to ascertain their diagnostic value.AIM To evaluate the pooled diagnostic performance of PCT and presepsin in detecting BI among patients with cirrhosis.INTRODUCTION Bacterial infections(BI)commonly occur in patients with cirrhosis,resulting in poor outcomes,including the development of cirrhotic complications,septic shock,acute-on-chronic liver failure(ACLF),multiple organ failures,and mortality[1,2].BI is observed in 20%-30%of hospitalized patients,with and without ACLF[3].Patients with cirrhosis are susceptible to BI because of internal and external factors.The major internal factors are changes in gut microbial composition and function,bacterial translocation,and cirrhosis-associated immune dysfunction syndrome[4,5].External factors include alcohol use,proton-pump inhibitor use,frailty,readmission,and invasive procedures.Spontaneous bacterial peritonitis(SBP),urinary tract infection,pneumonia,and primary bacteremia are the common BIs in hospit-alized patients with cirrhosis[6].Early diagnosis and adequate empirical antibiotic therapy are two critical factors that improve the prognosis of BI in patients with cirrhosis.However,early detection of BI in cirrhosis is challenging due to subtle clinical signs and symptoms,low sensitivity and specificity of systemic inflammatory response syndrome criteria,and low sensitivity of bacterial cultures.Thus,effective biomarkers need to be identified for the early detection of BI.Several biomarkers have been evaluated,but their efficacy in detecting BI is unclear.Procalcitonin(PCT)is a precursor of the hormone calcitonin,which is secreted by parafollicular cells of the thyroid gland[7].In the presence of BI,PCT gene expression increases in extrathyroidal tissues,causing a subsequent increase in serum PCT level[8].Changes in serum PCT are detectable as early as 4 hours after infection onset and peaks between 8 and 24 hours,making it a valuable diagnostic biomarker for BI.Several studies have demonstrated the favorable diagnostic accuracy of PCT in the diagnosis of BI in individuals with cirrhosis[9-13]and without cirrhosis[14-16].Since 2014,two meta-analyses have been published on the diagnostic value of PCT for SBP and BI in patients with cirrhosis[17,18].Other related studies have been conducted since then[10-12,19-33].Serum presepsin has recently emerged as a promising biomarker for diagnosing BI.This biomarker is the N-terminal fraction protein of the soluble CD14 g-negative bacterial lipopolysaccharide–lipopolysaccharide binding protein(sCD14-LPS-LBP)complex,which is cleaved by inflammatory serum protease in response to BI[34].Presepsin levels increase within 2 hours and peaks in 3 hours[35].This is useful for detecting BI since presepsin levels increase earlier than serum Our systematic review and meta-analysis was performed with adherence to PRISMA guidelines[37].
基金supported by the STI 2030 Major Projects(2022ZD0211700)the Key R&D Program of Guangdong Province,China(2018B030339001)+2 种基金the Key Realm R&D Program of Guangzhou,China(202007030007)the National Natural Science Foundation of China(82371538)The authors gratefully acknowledge the approval granted by the Ethics Committee of the Affiliated Brain Hospital of Guangzhou Medical University for this study involving human participants,with the approval ID(2021)No.071.
文摘Depression is increasingly prevalent among adolescents and can profoundly impact their lives.However,the early detection of depression is often hindered by the timeconsuming diagnostic process and the absence of objective biomarkers.In this study,we propose a novel approach for depression detection based on an affective brain-computer interface(aBCI)and the resting-state electroencephalogram(EEG).By fusing EEG features associated with both emotional and resting states,our method captures comprehensive depression-related information.The final depression detection model,derived through decision fusion with multiple independent models,further enhances detection efficacy.Our experiments involved 40 adolescents with depression and 40 matched controls.The proposed model achieved an accuracy of 86.54%on cross-validation and 88.20%on the independent test set,demonstrating the efficiency of multi-modal fusion.In addition,further analysis revealed distinct brain activity patterns between the two groups across different modalities.These findings hold promise for new directions in depression detection and intervention.
基金supported by the National Natural Science Foundation of China(No.62276204)the Fundamental Research Funds for the Central Universities,China(No.YJSJ24011)+1 种基金the Natural Science Basic Research Program of Shaanxi,China(Nos.2022JM-340 and 2023-JC-QN-0710)the China Postdoctoral Science Foundation(Nos.2020T130494 and 2018M633470)。
文摘Visible and infrared(RGB-IR)fusion object detection plays an important role in security,disaster relief,etc.In recent years,deep-learning-based RGB-IR fusion detection methods have been developing rapidly,but still struggle to deal with the complex and changing scenarios captured by drones,mainly due to two reasons:(A)RGB-IR fusion detectors are susceptible to inferior inputs that degrade performance and stability.(B)RGB-IR fusion detectors are susceptible to redundant features that reduce accuracy and efficiency.In this paper,an innovative RGB-IR fusion detection framework based on global-local feature optimization,named GLFDet,is proposed to improve the detection performance and efficiency of drone-captured objects.The key components of GLFDet include a Global Feature Optimization(GFO)module,a Local Feature Optimization(LFO)module and a Channel Separation Fusion(CSF)module.Specifically,GFO calculates the information content of the input image from the frequency domain and optimizes the features holistically.Then,LFO dynamically selects high-value features and filters out low-value features before fusion,which significantly improves the efficiency of fusion.Finally,CSF fuses the RGB and IR features across the corresponding channels,which avoids the rearrangement of the channel relationships and enhances the model stability.Extensive experimental results show that the proposed method achieves the best performance on three popular RGB-IR datasets Drone Vehicle,VEDAI,and LLVIP.In addition,GLFDet is more lightweight than other comparable models,making it more appealing to edge devices such as drones.The code is available at https://github.com/lao chen330/GLFDet.
文摘Driver distraction is a leading cause of traffic accidents,with fatigue being a significant contributor.This paper introduces a novel method for detecting driver distraction by analyzing facial features using machine deep learning and 68 face model.The proposed system assesses driver tiredness by measuring the distance between key facial landmarks,such as the distance between the eyes and the angle of the mouth,to evaluate signs of drowsiness or disengagement.Real-time video feed analysis allows for continuous monitoring of the driver’s face,enabling the system to detect behavioral cues associated with distraction,such as eye closures or changes in facial expressions.The effectiveness of this method is demonstrated through a series of experiments on a dataset of driver videos,which proves that the approach can accurately assess tiredness and distraction levels under various driving conditions.By focusing on facial landmarks,the system is computationally efficient and capable of operating in real-time,making it a practical solution for in-vehicle safety systems.This paper discusses the system’s performance,limitations,and potential for future enhancements,including integration with other in-vehicle technologies to provide comprehensive driver monitoring.
文摘Detection of Autism Spectrum Disorder(ASD)is a crucial area of research,representing a foundational aspect of psychological studies.The advancement of technology and the widespread adoption of machine learning methodologies have brought significant attention to this field in recent years.Interdisciplinary efforts have further propelled research into detection methods.Consequently,this study aims to contribute to both the fields of psychology and computer science.Specifically,the goal is to apply machine learning techniques to limited data for the detection of Autism Spectrum Disorder.This study is structured into two distinct phases:data preprocessing and classification.In the data preprocessing phase,four datasets—Toddler,Children,Adolescent,and Adult—were converted into numerical form,adjusted as necessary,and subsequently clustered.Clustering was performed using six different methods:Kmeans,agglomerative,DBSCAN(Density-Based Spatial Clustering of Applications with Noise),mean shift,spectral,and Birch.In the second phase,the clustered ASD data were classified.The model’s accuracy was assessed using 5-fold cross-validation to ensure robust evaluation.In total,ten distinct machine learning algorithms were employed.The findings indicate that all clustering methods demonstrated success with various classifiers.Notably,the K-means algorithm emerged as particularly effective,achieving consistent and significant results across all datasets.This study is expected to serve as a guide for improving ASD detection performance,even with minimal data availability.
基金supported by the Scientific Research Innovation Capability Support Project for Young Faculty (Grant No.ZYGXQNJSKYCXNLZCXMI3)the National Key Research and Development Program of China (Grant No.2022YFA1604402)+1 种基金the National Natural Science Foundation of China (Grant Nos.U23A6002,92250307,and 52225106)the Beijing Municipal Science and Technology Commission,Administrative Commission of Zhongguancun Science Park (Grant No.Z25110000692500)。
文摘Strong-field terahertz(THz) radiation holds significant potential in non-equilibrium state manipulation, electron acceleration, and biomedical effects. However, distortion-free detection of strong-field THz waveforms remains an essential challenge in THz science and technology. To address this issue, we propose a ferromagnetic detection scheme based on Zeeman torque sampling, achieving distortion-free strong-field THz waveform detection in Py films. Thickness-dependent characterization(3–21 nm) identifies peak detection performance at 21 nm within the investigated range. Furthermore, by structurally engineering the Py ferromagnetic layer, we demonstrate strong-field THz detection in symmetric Ta(3 nm)/Py(9 nm)/Ta(3 nm) heterostructure while simultaneously resolving Zeeman torque responses and collective spin-wave dynamics in asymmetric W(4 nm)/Py(9 nm)/Pt(2 nm)heterostructure. We calculated spin wave excitations and spin orbit torque distributions in asymmetric heterostructures, along with spin wave excitations in symmetric modes. This approach overcomes the sensitivity limitations of conventional techniques in strong-field conditions.
文摘Anomaly detection is an important research area in a diverse range of real-world applications.Although many algorithms have been proposed to address anomaly detection for numerical datasets,categorical and mixed datasets remain a significant challenge,primarily because a natural distance metric is lacking.Consequently,the methods proposed in the literature implement entirely different assumptions regarding the definition of cate-gorical anomalies.This paper presents a novel categorical anomaly detection approach,offering two key con-tributions to existing methods.First,a novel surprisal-based anomaly score is introduced,which provides a more accurate assessment of anomalies by considering the full distribution of categorical values.Second,the proposed method considers complex correlations in the data beyond the pairwise interactions of features.This study proposed and tested the novel categorical surprisal anomaly detection algorithm(CSAD)by comparing and evaluating it against six competitors.The experimental results indicate that CSAD produced the best overall performance,achieving the highest average ROC-AUC and PR-AUC values of 0.8 and 0.443,respectively.Furthermore,CSAD's execution time is satisfactory even when processing large,high-dimensional datasets.
文摘At an age when most teens are figuring out high school,Siddharth is already shaping the future of medical tech.The 14⁃year⁃old boy from Dallas has created an AI⁃powered app,Circadian AI,capable of detecting heart disease in just 7 seconds using only a smartphone's microphone.
文摘Non-cooperative communication detection is a key technology for locating radio interfer-ence sources and conducting reconnaissance on adversary radiation sources.To meet the require-ments of wide-area monitoring,a single interception channel often contains mixed multi-source sig-nals and interference,resulting in generally low signal-to-noise ratio(SNR)of the received signals;meanwhile,improving detection quality urgently requires either high frequency resolution or high-time resolution,which poses severe challenges to detection techniques based on time-frequency rep-resentations(TFR).To address this issue,this paper proposes a fixed-frame-structure signal detec-tion algorithm that integrates image enhancement and multi-scale template matching:first,the Otsu-Sauvola hybrid thresholding algorithm is employed to enhance TFR features,suppress noise interference,and extract time-frequency parameters of potential target signals(such as bandwidth and occurrence time);then,by exploiting the inherent time-frequency characteristics of the fixed-frame structure,the signal is subjected to multi-scale transformation(with either high-frequency resolution or high-time resolution),and accurate detection is achieved through the corresponding multi-scale template matching.Experimental results demonstrate that under 0 dB SNR conditions,the proposed algorithm achieves a detection rate greater than 87%,representing a significant improvement over traditional methods.
基金supported by National Natural Science Foundation of China(Nos.22174016,22374019,and 22209025)Natural Science Foundation of Jiangsu Province(No.BK20220799).
文摘Nanochannel technology based on ionic current rectification has emerged as a powerful tool for the detection of biomolecules owing to unique advantages.Nevertheless,existing nanochannel sensors mainly focus on the detection of targets in solution or inside the cells,moreover,they only have a single function,greatly limiting their application.Herein,we fabricated SuperDNA self-assembled conical nanochannel,which was clamped in the middle of self-made device for two functions:Online detecting living cells released TNF-αand studying intercellular communication.Polyethylene terephthalate(PET)membrane incubated tumor associated macrophages and tumor cells was rolled up and inserted into the left and right chamber of the device,respectively.Through monitoring the ion current change in the nanochannel,tumor associated macrophages released TNF-αcould be in situ and noninvasive detected with a detection limit of 0.23 pg/mL.Furthermore,the secreted TNF-αinduced epithelial-mesenchymal transformation of tumor cells in the right chamber was also studied.The presented strategy displayed outstanding performance and multi-function,providing a promising platform for in situ non-destructive detection of cell secretions and related intercellular communication analysis.
基金supported by the National Natural Science Foundation of China(Nos.22225806,22378385,22078314,22278394)Dalian Institute of Chemical Physics(Nos.DICPI202142,DICPI202436,DMU-1&DICP UN202301).
文摘The continuous mutation and rapid spread of the severe acute respiratory syndrome coronavirus 2(SARSCoV-2)have led to the ineffectiveness of many antiviral drugs targeting the original strain.To keep pace with the virus’evolutionary speed,there is a crucial need for the development of rapid,cost-effective,and efficient inhibitor screening methods.In this study,we created a novel approach based on fluorescence resonance energy transfer(FRET)technology for in vitro detection of inhibitors targeting the interaction between the SARS-CoV-2 spike protein RBD(s-RBD)and the virus receptor angiotensin-converting enzyme 2(ACE2).Utilizing crystallographic insights into the s-RBD/ACE2 interaction,we modified ACE2 by fusing SNAP tag to its N-terminus(resulting in SA740)and Halo tag to s-RBD’s C-terminus(producing R525H and R541H),thereby ensuring the proximity(<10 nm)of labeled FRET dyes.We found that relative to the R541H fusion protein,R525H exhibited higher FRET efficiency,which attributed to the shortened distance between FRET dyes due to the truncation of s-RBD.Utilizing the sensitive FRET effect between SA740 and R525H,we evaluated its efficacy in detecting inhibitors of SARS-CoV-2 entry in solution and live cells.Ultimately,this FRET-based detection method was demonstrated high sensitivity,rapidity,and simplicity in solution and held promise for high-throughput screening of SARS-CoV-2 inhibitors.
