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Fractal characterization for the mining crack evolution process of overlying strata based on microseismic monitoring technology 被引量:5
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作者 Liu Chao Xue Junhu +1 位作者 Yu Guofeng Cheng Xiaoyu 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2016年第2期295-299,共5页
In order to study the evolution laws during the development process of the coal face overburden rock mining-induced fissure,we studied the process of evolution of overburden rock mining-induced fissures and dynamicall... In order to study the evolution laws during the development process of the coal face overburden rock mining-induced fissure,we studied the process of evolution of overburden rock mining-induced fissures and dynamically quantitatively described its fractal laws,based on the high-precision microseismic monitoring method and the nonlinear Fractal Geometry Theory.The results show that:the overburden rock mining-induced fissure fractal dimension experiences two periodic change processes with the coal face advance,namely a Small→ Big→ Small process,which tends to be stable;the functional relationship between the extraction step distance and the overburden rock mining-induced fissure fractal dimension is a cubic curve.The results suggest that the fractal dimension reflects the evolution characteristics of the overburden rock mining-induced fissure,which can be used as an evaluation index of the stability of the overburden rock strata,and it provides theoretical guidance for stability analysis of the overburden rock strata,goaf roof control and the support movements in the mining face. 展开更多
关键词 Microseismic monitoring technology Fractal characterization Mining crack Evolution law Overlying strata
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Functional Characterization of Bifidobacteria of Human Origin: A Case Study by the Students of Food Science and Technology of the University of Foggia (Southern Italy)
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作者 Emanuela Ciuffreda Angino Veronica +12 位作者 Antonietta Cifelli Rossana Foti Rosaria Ilenia Forte Fabio Graziani Andrea Giuseppe Longo Angela Longo Valeria Maglia Emilio Francesco Ricciardi Alessandra Sabatino Annamaria Tomaiuolo Maria Rosaria Corbo Milena Sinigaglia Antonio Bevilacqua 《Food and Nutrition Sciences》 2014年第13期1153-1161,共9页
The aim of this paper was to study the potential technological and probiotic properties of bifidobacteria isolated from human feces. Bifidobacteria, naturally present in the dominant colonic microbiota, represent up t... The aim of this paper was to study the potential technological and probiotic properties of bifidobacteria isolated from human feces. Bifidobacteria, naturally present in the dominant colonic microbiota, represent up to 25% of the cultivable faecal bacteria in adults and 80% in infants. Bifidobacteria have been shown to adhere and colonize in high numbers different types of cultured intestinal epithelial cells;moreover some authors reported that some strains are able to stabilize the intestinal microbiota during and after antibiotic therapy, modulate the immune system, protecting against chemically induced intestinal inflammation and reducing symptoms of colitis. Eight isolates of bifidobacteria were studied to assess their technological and probiotic traits;the technological characterization relied on the assessment of enzymatic activities (proteolytic and lipolytic activity), growth under various conditions (pH, temperature and addition of salt), acidifying ability and metabolism (arginine deamination, esculin, esculin hydrolysis and citrate metabolism). The study of the probiotic characteristics focused on the evaluation of the survival at low pH and with bile salts added, antibiotic resistance, and hydrophobic properties. As a result of this process, two promising strains were selected for further studies. 展开更多
关键词 BIFIDOBACTERIA technology Probiotic TRAITS FUNCTIONAL characterization
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Fine Characterization and Analysis of Drying Strain of the ELM Board via DIC Technology
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作者 Yuanchu Liu Xiaodong Zhu +5 位作者 Zhengmin Jin Yingying Liu Qingjian Wei Bonan Liang Yingchun Cai Jingyao Zhao 《Journal of Renewable Materials》 SCIE EI 2023年第2期567-580,共14页
In this paper,the occurrence and development mechanism of strain on the cross-section during the wood drying is explored.Therefore,strain regularity on the cross-section of 50 mm thickness elm(Ulmus rubra)board at the... In this paper,the occurrence and development mechanism of strain on the cross-section during the wood drying is explored.Therefore,strain regularity on the cross-section of 50 mm thickness elm(Ulmus rubra)board at the temperature of 40℃and 80℃is detected via digital image correlation technology.Hence,the difference between tangential and radial strain at surface and core layers was denoted.The results showed that strain distribution in the width direction of the board is uneven.Moreover,a large drying shrinkage strain occurs at the near-core layer,while the maximum strain difference reaches 4.08%.Hence,the surface of the board is cracked along the thickness direction.The radial strain of the board is higher than the tangential strain in the early stage of drying,while these strains are reversed in the later stage of drying.The temperature is related to the difference between the tangential and radial strains of the elm board.These differences at the core layer are larger than those of the surface layer.The conducted research results provide a theoretical basis for process optimization. 展开更多
关键词 Digital image correlation technology drying strain fine characterization moisture content distribution
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Major Cultivation Technology of HQ No.1, a New Flue-cured Tobacco Cultivar
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作者 李文宇 李爱华 《Agricultural Science & Technology》 CAS 2015年第3期497-499,共3页
The transplanting period, density, the number of left leaves and fertilizer amounts of HQ No.1 were explored in Hengyang. The results showed that with transplanting periods of March 5-March 15, and planting space of 1... The transplanting period, density, the number of left leaves and fertilizer amounts of HQ No.1 were explored in Hengyang. The results showed that with transplanting periods of March 5-March 15, and planting space of 120 cmx(50-60) cm, agronomic and economic characters of tobaccos showed insignificant differences. Specifically, plant height was growing upon the number of left leaf and fertilizer amount; tobacco yield and output value kept increasing upon fertilizer, and both reached maximums with pure N at 195 kg/hm^2, showing significant differences with the treatment of pure N at 135 kg/hm^2; the number of left leaf had the least effects on agronomic and economic characters of tobaccos. The research indicated that highly-qualified HQ No.1 can be produced, given that tobaccos are transplanted during March 10-March 15, planting density of 15 000-16 500 seedlings/hm^2, the number of left leaves of 22-24 leaves per seedling, and pure N of 150-165 kg/hm^2 in Hengyang. 展开更多
关键词 Flue-cured tobaccos HQ No.1 Major cultivation technology Agronomic characters Economic character Tolerance to disease
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Decoding lithium batteries through advanced in situ characterization techniques 被引量:11
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作者 Mei Yang Ruyi Bi +2 位作者 Jiangyan Wang Ranbo Yu Dan Wang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2022年第5期965-989,共25页
Given the energy demands of the electromobility market,the energy density and safety of lithium batteries(LBs)need to be improved,whereas its cost needs to be decreased.For the enhanced performance and decreased cost,... Given the energy demands of the electromobility market,the energy density and safety of lithium batteries(LBs)need to be improved,whereas its cost needs to be decreased.For the enhanced performance and decreased cost,more suitable electrode and electrolyte materials should be developed based on the improved understanding of the degradation mechanisms and structure–performance correlation in the LB system.Thus,various in situ characterization technologies have been developed during the past decades,providing abundant guidelines on the design of electrode and electrolyte materials.Here we first review the progress of in situ characterization of LBs and emphasize the feature of the multi-model coupling of different characterization techniques.Then,we systematically discuss how in situ characterization technologies reveal the electrochemical processes and fundamental mechanisms of different electrode systems based on representative electrode materials and electrolyte components.Finally,we discuss the current challenges,future opportunities,and possible directions to promote in situ characterization technologies for further improvement of the battery performance. 展开更多
关键词 in situ characterization techniques multi-modal coupling lithium batteries electrochemical mechanism
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Spatial and seasonal characterization of net primary productivity and climate variables in southeastern China using MODIS data 被引量:10
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作者 Dai-liang PENG Jing-feng HUANG +6 位作者 Alfredo R. HUETE Tai-ming YANG Ping GAO Yan-chun CHEN Hui CHEN Jun LI Zhan-yu LIU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第4期275-285,共11页
We developed a sophisticated method to depict the spatial and seasonal characterization of net primary productivity (NPP) and climate variables. The role of climate variability in the seasonal variation of NPP exerts ... We developed a sophisticated method to depict the spatial and seasonal characterization of net primary productivity (NPP) and climate variables. The role of climate variability in the seasonal variation of NPP exerts delayed and continuous effects. This study expands on this by mapping the seasonal characterization of NPP and climate variables from space using geographic information system (GIS) technology at the pixel level. Our approach was developed in southeastern China using moderate-resolution imaging spectroradiometer (MODIS) data. The results showed that air temperature,precipitation and sunshine percentage contributed significantly to seasonal variation of NPP. In the northern portion of the study area,a significant positive 32-d lagged correlation was observed between seasonal variation of NPP and climate (P<0.01),and the influences of changing climate on NPP lasted for 48 d or 64 d. In central southeastern China,NPP showed 16-d,48-d,and 96-d lagged correlation with air temperature,precipitation,and sunshine percentage,respectively (P<0.01); the influences of air temperature and precipitation on NPP lasted for 48 d or 64 d,while sunshine influence on NPP only persisted for 16 d. Due to complex topography and vegetation distribution in the southern part of the study region,the spatial patterns of vegetation-climate relationship became complicated and diversiform,especially for precipitation influences on NPP. In the northern part of the study area,all vegetation NPP had an almost similar response to seasonal variation of air temperature except for broad crops. The impacts of seasonal variation of precipitation and sunshine on broad and cereal crop NPP were slightly different from other vegetation NPP. 展开更多
关键词 Net primary productivity Climate variables Spatial characterization Lagged cross-correlation Moderate-resolution imaging spectroradiometer Geographic information system technology
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Rock mass structural recognition from drill monitoring technology in underground mining using discontinuity index and machine learning techniques 被引量:6
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作者 Alberto Fernández JoséA.Sanchidrián +3 位作者 Pablo Segarra Santiago Gómez Enming Li Rafael Navarro 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2023年第5期555-571,共17页
A procedure to recognize individual discontinuities in rock mass from measurement while drilling(MWD)technology is developed,using the binary pattern of structural rock characteristics obtained from in-hole images for... A procedure to recognize individual discontinuities in rock mass from measurement while drilling(MWD)technology is developed,using the binary pattern of structural rock characteristics obtained from in-hole images for calibration.Data from two underground operations with different drilling technology and different rock mass characteristics are considered,which generalizes the application of the methodology to different sites and ensures the full operational integration of MWD data analysis.Two approaches are followed for site-specific structural model building:a discontinuity index(DI)built from variations in MWD parameters,and a machine learning(ML)classifier as function of the drilling parameters and their variability.The prediction ability of the models is quantitatively assessed as the rate of recognition of discontinuities observed in borehole logs.Differences between the parameters involved in the models for each site,and differences in their weights,highlight the site-dependence of the resulting models.The ML approach offers better performance than the classical DI,with recognition rates in the range 89%to 96%.However,the simpler DI still yields fairly accurate results,with recognition rates 70%to 90%.These results validate the adaptive MWD-based methodology as an engineering solution to predict rock structural condition in underground mining operations. 展开更多
关键词 Drill monitoring technology Rock mass characterization Underground mining Similarity metrics of binary vectors Structural rock factor Machine learning
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Bifidobacteria as Potential Functional Starter Cultures: A Case Study by MSc Students in Food Science and Technology (University of Foggia, Southern Italy) 被引量:2
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作者 Antonio Bevilacqua Maria Teresa Cagnazzo +7 位作者 Cataldo Caldarola Emanuela Ciuffreda Anna Rita Dragano Stefania Franchino Rossana Lauriola Angela Pacifico Maria Rosaria Corbo Milena Sinigaglia 《Food and Nutrition Sciences》 2012年第1期55-63,共9页
This research paper was the results of activity of MSc students of Food Science and Technology, attending the class “Biotechnology of Functional Starter”. Five strains of bifidobacteria (Bifidobacterium animalis sub... This research paper was the results of activity of MSc students of Food Science and Technology, attending the class “Biotechnology of Functional Starter”. Five strains of bifidobacteria (Bifidobacterium animalis subsp. lactis;B. longum subsp. infantis;B. breve;B. animalis subsp. animalis;B. bifidum) were evaluated in order to assess their suitability as functional starter cultures, by studying the following technological and probiotic traits: growth at different temperatures, NaCl amounts and pH values;acidifying ability;metabolism (arginin deamination, esculin hydrolysis, acetoin production);survival at low pH and in presence of bile salts;hydrophobic properties;antibiotic resistance. After laboratory assays and strain selection through a multivariate analyses, it was highlighted that B. longum subsp. infantis and B. animalis subsp. lactis represent a good compromise as potential functional starter cultures, as B. animalis subp. lactis showed a high growth index at pH 5 and good values at 25?C and 30?C, as well as the minimal viability loss at pH 2.5. B. longum subsp. infantis DSMZ 20088 was the best microorganism for its growth index in presence of 6.5% of salt added and at 25?C and 30?C. 展开更多
关键词 BIFIDOBACTERIA FUNCTIONAL STARTER Probiotic PROPERTIES technologICAL characterization
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Preparation and characterization of uniform circinate aggregates of sheet ZnO nanoparticles 被引量:1
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作者 LIU Jiaxiang DING Deling +1 位作者 WANG Lisheng and HAN Yuexin 《Rare Metals》 SCIE EI CAS CSCD 2008年第1期36-40,共5页
Uniform circinate aggregates of sheet ZnO nanoparticles with a specific surface area of 24 m^2·g^-1 were prepared by the direct precipitation method.The circinate aggregates were hexagonal ZnO,with the wall thick... Uniform circinate aggregates of sheet ZnO nanoparticles with a specific surface area of 24 m^2·g^-1 were prepared by the direct precipitation method.The circinate aggregates were hexagonal ZnO,with the wall thickness ranging from 0.5 to 1 μm and the diameter ranging from 5 to 10 μm.The backs of the circinate aggregates were regularly arranged by numerous sheet ZnO nanoparticles with a thickness of 30-80 nm and a diameter of 300-400 nm.The precursors were aggregates of sheet Zn5(CO3)2(OH)6 nanoparticles,which were decomposed into ZnO by calcining in air in the range of 200-285°C,and the ZnO retained the similar special structure.The C2H5OH content had a significant effect on the formation of sheet ZnO.The centripetal force,the Van der Waals force,and the hydrogen bond were deduced as the driving forces of the formation of circinate. 展开更多
关键词 NANO-ZNO circinate aggregates preparation technology MICROSTRUCTURE characterization
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Foundry Coating Technology: A Review 被引量:5
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作者 U. C Nwaogu N. S Tiedje 《Materials Sciences and Applications》 2011年第8期1143-1160,共18页
The importance of foundry coating in improving the surface quality of castings cannot be over emphasized. The application of mould and core washes creates a high thermal integrity barrier between the metal and the mou... The importance of foundry coating in improving the surface quality of castings cannot be over emphasized. The application of mould and core washes creates a high thermal integrity barrier between the metal and the mould resulting in the reduction of the thermal shock experienced by the sand system. These thermal shock leads to series of surface defects such as veining/finning, metal penetration, burn-on/in, scab, rat tail, erosion etc. The use of coatings reduces the tendency of occurrence of these defects. However, the understanding of the coating, its components, characteristics and mechanism of action is important. In this review, a detailed description of these topics and examples are provided where necessary. A potential area of research in foundry coating development, using sol-gel process is suggested. The application of sol-gel technology in the development of foundry coatings is a novel approach. 展开更多
关键词 COATING REFRACTORY Materials Application METHODS characterization SOL-GEL technology
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Characterization of Fundamental Logics for the Sub-Threshold Digital Design
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作者 Yan-Ming He Ya-Juan He +2 位作者 Yang-Ming Li Shao-Wei Zhen Ping Luo 《Journal of Electronic Science and Technology》 CAS 2013年第4期382-387,共6页
Digital circuits operating in the sub-threshold regime consume the least energy. The strict energy constraints are desired in the applications which work at the lowest possible supply voltage. On the other hand, the c... Digital circuits operating in the sub-threshold regime consume the least energy. The strict energy constraints are desired in the applications which work at the lowest possible supply voltage. On the other hand, the conventional design flow utilizes the technology library provided by the foundry with a fixed voltage boundary, which causes problems when the supply scales down to the sub-threshold regime. In this paper, we present a design methodology to characterize the existing cell library with Liberty NCX to facilitate the standard design flow. It is demonstrated in 0.13 μm complementary metal-oxide-semiconductor (CMOS) technology with the supply voltage of 300 mV. 展开更多
关键词 characterization digital circuit LOWPOWER sub-threshold voltage technology library.
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Breeding and High-yield Cultivation Technology of Wuqing No.1, a New Cultivar of Black Soybeans
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作者 谢成林 姚义 +1 位作者 马长青 冯明 《Agricultural Science & Technology》 CAS 2015年第11期2519-2521,共3页
The research introduced selection and breeding process, characteristics and high-yield cultivation technology of Wuqing No.l. Wuqing No.l, also named Gaoyou black soybean, is bred based on Danbo black soybean introduc... The research introduced selection and breeding process, characteristics and high-yield cultivation technology of Wuqing No.l. Wuqing No.l, also named Gaoyou black soybean, is bred based on Danbo black soybean introduced from Japan in 1996 by Gaoyou Vegetable Cultivation Technology Station and Yangzhou Agricultural Technology Promotion Station. It was named in 2014 by Jinagsu Crop Cultivar Approvement Committee. The cultivar is high-yielding with high quality, big seeds, high resistance to stress, as well as high health-care value. 展开更多
关键词 Black soybean Wuqing No.l Selection and breeding character Cultiva-tion technology
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Differences Among Industrial Companies in Their Innovative Efforts and Competitiveness: On How Size, Technological Level and Subcontractor Character Matter
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作者 Ricardo Martinez Santa Maria Jon Charterina Abando Andres Araujo de la Mata 《Chinese Business Review》 2011年第3期187-204,共18页
The aim of this research is to test to what extent do the classifying variables of company size, technological level and subcontractor nature moderate on the causal relationship between a finn's innovative capacities... The aim of this research is to test to what extent do the classifying variables of company size, technological level and subcontractor nature moderate on the causal relationship between a finn's innovative capacities and its competitiveness. To this end, we use a random sample of 861 manufactttring f'Lrms with 10 or more employees from the Basque Autonomous Community (in Spain), accounting for 26.29% of the population. Maximum sampling error is 2.87% for a 95% confidence interval. We start from a competitiveness general model of the firm, and set out under the resource based view of the finn, which had been previously tested and validated by Martinez (2009) for the whole sample. The model is formed with the constructs of management capabilities, innovative capabilities, marketing capabilities, quality capabilities, current competitiveness and future competitiveness. From this point, we test a set of alternative hierarchical models both for the total sample and for different divisions in sub-samples, according to the intervals of size in number of employees, OECD technological levels and firms' subcontractor character. The innovative capabilities construct is reflected in the items of radical product innovation, incremental product innovation, innovation in the production process, innovation in marketing, management innovation and the efforts in company workers' training and development. From all these items, previous descriptive data analysis showed that although product radical innovation was the most representative capability for superior innovativeness, it was at the same time the least evident from all the aforementioned items in the case of the analyzed Basque industrial companies. The analysis of results led us to conclude that the factor of innovative capabilities was the most influential on current competitiveness among the whole sample. In particular, as regards the size factor, this positive effect dilutes in the case of smaller companies. In the same manner, whereas in the case of the companies with higher technological level it is verified that the factor of innovative capabilities reveals crucial, in the group of fhans with lower technological level the most relevant factor is quality. Also, the same effect is evidenced for the case of the subcontractor/non-subcontractor nature of the finns: The first ones show quality as the most relevant construct while for the second ones it is the innovative capabilities. 展开更多
关键词 COMPETITIVENESS innovative capabilities company size technological level subcontractor character
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页岩油赋存状态表征技术研究进展与展望
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作者 李志明 孙中良 +11 位作者 梁涛 常佳琦 申宝剑 冷筠滢 曹婷婷 李政 包友书 李楚雄 钱门辉 黎茂稳 贾梦瑶 杜心宇 《石油实验地质》 北大核心 2026年第1期61-73,共13页
页岩油赋存状态表征是众多学者和勘探家关注的热点问题,也是页岩油勘探开发评价中面临的难点问题。在系统梳理总结当前页岩油赋存空间可视化与定量化表征、不同赋存状态油/烃定量表征实验技术系列以及分子动力学模拟法表征技术研究进展... 页岩油赋存状态表征是众多学者和勘探家关注的热点问题,也是页岩油勘探开发评价中面临的难点问题。在系统梳理总结当前页岩油赋存空间可视化与定量化表征、不同赋存状态油/烃定量表征实验技术系列以及分子动力学模拟法表征技术研究进展的基础上,阐述了主要表征技术的优势与不足,提出了应用过程中需要注意的事项,探讨了页岩油赋存状态表征技术重点攻关方向。在获取密闭保压取心岩心且采用液氮冷冻保存样品基础上,未来页岩油赋存状态表征技术攻关应聚焦于以下4个方面:(1)超低温荧光光谱、激光扫描共聚焦显微镜和环境扫描电镜页岩油赋存特征可视化表征技术及其联用技术;(2)页岩纹层原位微区滞留油赋存状态与组分在线分析技术;(3)超低温与地质条件下二维核磁共振流体赋存空间与赋存状态定量表征技术;(4)近地质条件下多尺度孔缝系统内页岩油赋存状态分子动力学模拟技术。通过多技术联用与技术创新来推进页岩油赋存状态表征技术的完善与发展,以实现页岩油赋存空间、赋存形态、赋存状态与组分近原位、可视化与定量化表征,为高效支撑我国陆相富有机质泥页岩层系含油性、赋存状态与可动性评价以及页岩油甜点层段的优选、资源量的合理评估与分级评价提供可靠的科学依据。 展开更多
关键词 页岩油 赋存状态 表征技术 可视化 定量表征
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间充质干细胞来源外泌体分离、鉴定技术及应用 被引量:2
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作者 刘宇 龚森怡 +4 位作者 杨丽华 李伟风 胡玉雯 闫钦彪 郭美锦 《中国组织工程研究》 北大核心 2026年第1期194-203,共10页
背景:间充质干细胞外泌体因低免疫原性和靶向递送效果在细胞通讯和表观遗传调控中发挥重要作用,已在疾病的治疗中临床应用。目的:综述间充质干细胞外泌体的分离纯化、鉴定方法及应用进展,促进间充质干细胞外泌体大规模制备技术的开发与... 背景:间充质干细胞外泌体因低免疫原性和靶向递送效果在细胞通讯和表观遗传调控中发挥重要作用,已在疾病的治疗中临床应用。目的:综述间充质干细胞外泌体的分离纯化、鉴定方法及应用进展,促进间充质干细胞外泌体大规模制备技术的开发与临床转化。方法:以“外泌体,间充质干细胞,分离纯化,表征,临床应用”为中文检索词,“exosome,extracellular vesicles,mesenchymal stem cells,isolation,characterization,application”为英文检索词,检索中国知网、PubMed和Web of Science数据库于2024年9月之前发表的文献,排除与主题相关性较差、年代久远及重复的文章,最后纳入109篇文献进行综述。结果与结论:①综述了近年来外泌体分离纯化方法,根据原理的不同比较了超速离心法、超滤法、尺寸排阻色谱法、聚合物沉淀法、免疫亲和法、微流控法及其他新方法的特点;②外泌体的鉴定方法可以分为物理与生化分析,从外泌体的形状大小及特征蛋白等方面进行表征;③间充质干细胞外泌体因具有免疫调节、跨越生物屏障等能力在医疗美容、损伤修复、癌症治疗等多个领域具有广泛应用;④外泌体由于自身结构复杂、缺少通用分离技术、稳定性较差等缺点而难以在短时间内实现临床转化。 展开更多
关键词 间充质干细胞 外泌体 细胞外囊泡 分离纯化 制备工艺 特征鉴定 临床应用 疾病治疗 工程化外泌体
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CD161单克隆抗体的研制及生物学特性鉴定
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作者 王昱涵 曹磊 +4 位作者 牛亚燕 胡玉敏 吕红艳 赵华阳 吴妹英 《中国免疫学杂志》 北大核心 2026年第2期446-450,共5页
目的:制备特异性CD161单克隆抗体,对其生物学特性进行检测与评估,并筛选出其中具有功能的单克隆抗体。方法:构建CD161/L929过表达细胞株;以CD161-His蛋白为免疫原,采用小鼠杂交瘤融合技术获得稳定分泌抗体的杂交瘤细胞株,通过流式细胞术... 目的:制备特异性CD161单克隆抗体,对其生物学特性进行检测与评估,并筛选出其中具有功能的单克隆抗体。方法:构建CD161/L929过表达细胞株;以CD161-His蛋白为免疫原,采用小鼠杂交瘤融合技术获得稳定分泌抗体的杂交瘤细胞株,通过流式细胞术和ELISA筛选抗体效价较高的杂交瘤细胞株进行多次亚克隆;用双阳性杂交瘤细胞株免疫小鼠,诱导腹水产生,制备单克隆抗体并纯化;筛选可用于Western blot和免疫组化的单克隆抗体;同时筛选可阻断CD161-LLT1结合的单克隆抗体。结果:成功构建了CD161/L929过表达细胞株。共筛选出40株可用于ELISA的单克隆抗体,29株可用于流式细胞术的单克隆抗体,6株可用于Western blot的单克隆抗体,4株可用于免疫组化的单克隆抗体;获得多株抗体可阻断CD161-LLT1结合的抗体,其中13A1、13A9和10A3亲和力较高,具有竞争阻断性。结论:本研究制备出多株特异性CD161单克隆抗体,筛选出可应用于各种免疫学实验的抗体,并筛选出其中具有阻断性的抗体,为进一步探究CD161发挥作用的机制提供物质基础。 展开更多
关键词 CD161 单克隆抗体 杂交瘤技术 生物学特性
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基于加压反应强化的锂电固废循环利用技术进展
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作者 于尚元 吕伟光 +1 位作者 孙峙 曹宏斌 《能源环境保护》 2026年第1期1-15,共15页
在全球能源转型与“双碳”目标推动下,锂电固废循环利用对保障资源供给安全与降低环境污染风险意义重大。加压技术凭借其改变热力学平衡、强化动力学过程以及反应效率高等优势,近年来在相关领域受到广泛关注。系统综述了加压技术在锂电... 在全球能源转型与“双碳”目标推动下,锂电固废循环利用对保障资源供给安全与降低环境污染风险意义重大。加压技术凭借其改变热力学平衡、强化动力学过程以及反应效率高等优势,近年来在相关领域受到广泛关注。系统综述了加压技术在锂电固废循环利用中的研究进展,尤其关注其在有价金属提取、材料再生制备及失效材料修复等方面的应用。具体来说,在有价金属提取方面,加压酸浸、氨浸及氧化浸出等技术受到关注,显著提升了锂、钴、镍等有价金属的回收效率;在材料再生制备方面,加压技术(水热法)可用于从净化后的浸出液中合成锂离子电池正极材料及其他高附加值功能材料;而在失效材料修复方面,加压技术则主要用于失效正极材料的补锂修复,以恢复其电化学性能。此外,针对加压过程原位表征难度大、相关表征技术缺失的现状,总结了原位同步辐射、拉曼光谱等先进表征技术在加压过程原位表征方面的研究进展。当前,加压过程存在热力学数据匮乏、机理认知不足,表征技术缺乏以及设备成本高等问题,未来应着重开展基础理论研究,完善热力学数据库,建立健全多维、多尺度原位表征方法体系,并耦合清洁能源,研发低成本设备。基于加压技术的创新突破,有望推动锂电固废循环利用领域低碳高效发展。 展开更多
关键词 加压技术 锂电固废 有价金属 高值材料 修复再生 原位表征
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基于病原体靶向测序技术的传染性单核细胞增多症患儿病原学特征及其与EB病毒载量的相关性分析
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作者 郑峥 赵艳男 王明硕 《河北医学》 2026年第1期144-150,共7页
目的:基于病原体靶向测序(tNGS)技术检测传染性单核细胞增多症(IM)患儿的病原体,分析其病原学特征及其与EB病毒载量的相关性。方法:回顾性选取2023年6月至2024年12月铜陵市第一人民医院和铜陵市妇幼保健院收治的46例由EB病毒导致IM患儿... 目的:基于病原体靶向测序(tNGS)技术检测传染性单核细胞增多症(IM)患儿的病原体,分析其病原学特征及其与EB病毒载量的相关性。方法:回顾性选取2023年6月至2024年12月铜陵市第一人民医院和铜陵市妇幼保健院收治的46例由EB病毒导致IM患儿作为观察组,同时选取同期本院收治的95例非IM患儿作为对照组。采用tNGS检测两组患者病原体,比较两组的病原学特征。结果:利用tNGS技术,在对照组中检出32种病原体,包括13种细菌、18种病毒以及1种支原体;而观察组中检出24种病原体,包括12种细菌、11种病毒以及1种衣原体。分析tNGS技术分离出的病原体,对照组中细菌检出率居前3位的为流感嗜血杆菌(n=40)、肺炎链球菌(n=29)、鲍曼不动杆菌(n=31);观察组前3位的细菌为流感嗜血杆菌(n=29)、肺炎链球菌(n=22)、金黄色葡萄球菌(n=21)。两组均检测出EB病毒,且观察组EB病毒载量高于对照组(P<0.05);相关性分析结果显示,金黄色葡萄球菌、卡他莫拉菌、鲍曼不动杆菌、肺炎克雷伯菌、咽峡炎链球菌与EBV病毒载量呈正相关关系(P<0.05)。结论:tNGS技术有助于全面了解IM患儿病原学特征,且病原学特征与EB病毒载量存在关联,为临床诊疗提供依据。 展开更多
关键词 病原体靶向测序技术 传染性单核细胞增多症 病原学特征 EB病毒载量
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鬼臼多糖的提取、结构表征及其生物活性
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作者 冯薇 李静洁 +3 位作者 李紫薇 蔺永刚 王俊龙 时文盼 《精细化工》 北大核心 2026年第1期132-143,163,共13页
为促进鬼臼多糖(Podophyllum emodi polysaccharides,PEP)的开发与应用,采用超声辅助离子液体法提取粗PEP,再经柱分离纯化获得两种均一组分的多糖PEP-Ⅰ和PEP-Ⅱ。通过单因素及响应面法优化了粗PEP的提取工艺,采用GPC、XRD、UV-Vis、FTI... 为促进鬼臼多糖(Podophyllum emodi polysaccharides,PEP)的开发与应用,采用超声辅助离子液体法提取粗PEP,再经柱分离纯化获得两种均一组分的多糖PEP-Ⅰ和PEP-Ⅱ。通过单因素及响应面法优化了粗PEP的提取工艺,采用GPC、XRD、UV-Vis、FTIR、SEM、TGA对PEP-Ⅰ和PEP-Ⅱ进行了表征,通过体外抗氧化、降糖实验考察了其生物活性。结果表明,粗PEP提取的最优条件为:以1-丁基-3-甲基咪唑氯盐(BMIMCl)为离子液体、液料比(mL∶g)40∶1、超声温度53.0℃、超声时间91.0 min、离子液体质量分数1.0%(以去离子水加入量计),在该条件下,粗PEP的提取率为37.796%±0.17%;PEP-Ⅰ是重均相对分子质量为27.32 kDa的α、β-构型的吡喃型中性多糖,具有三螺旋构象,主要由鼠李糖(61.85%,相对摩尔分数,下同)、甘露糖(19.36%)和阿拉伯糖(5.85%)组成的杂多糖,形态分布不均匀且部分呈网状;PEP-Ⅱ是重均相对分子质量为33.48 kDa的α、β-构型的吡喃型酸性多糖,主要由鼠李糖(52.89%)、木糖(12.63%)和葡萄糖(12.99%)组成,含有少量的蛋白质和糖醛酸,表面呈光滑片状薄膜,破碎程度较轻。PEP-Ⅰ和PEP-Ⅱ均为晶体与非晶体共存的晶体构象,且具有良好的热稳定性。PEP-Ⅰ和PEP-Ⅱ具有一定的抗氧化和降糖活性,对1,1-二苯基-2-三硝基苯肼自由基的最大半数抑制质量浓度(IC_(50))分别为1.832和0.734 g/L,对2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐阳离子自由基的IC_(50)分别为1.634和0.862 g/L,对羟基自由基的IC_(50)分别为1.941和0.926 g/L,对α-葡萄糖苷酶的IC_(50)分别为3.021和2.432 g/L,对α-淀粉酶的IC_(50)分别为3.242和2.398 g/L。 展开更多
关键词 鬼臼多糖 离子液体 超声提取 结构表征 生物活性 中药现代化技术
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