[Objectives]To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine.[Methods]High performance liquid chromatography(HPLC)w...[Objectives]To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine.[Methods]High performance liquid chromatography(HPLC)with ShimNex CS C 18 as the column;column temperature:35℃;wavelength:270 nm;methanol-0.1%phosphoric acid solution as the mobile phase with gradient elution.[Results]The 12 components of the retention of Longqing Capsule showed good linearity within the investigated range(r≥0.9995),with the average spiked recoveries of 97.83%-100.52%and the RSD of 0.9%-2.1%.[Conclusions]The method is exclusive,sensitive,reproducible,simple and easy to use,and can provide a reference for the construction of the quality standard and control system of Longqing Capsule based on the theory of traditional Chinese medicine.展开更多
Inductively coupled plasma optical emission spectrometric approach（1CP-OES） is used to determine the potassium content as principal component in pyrotechnic compositions used for fireworks and firecrackers. Element ...Inductively coupled plasma optical emission spectrometric approach（1CP-OES） is used to determine the potassium content as principal component in pyrotechnic compositions used for fireworks and firecrackers. Element of potassium is conunonly found in potassium nitrate and potassium perchlorate in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that potassium nitrate content in pyrotechnics is between 10% to 60% and the potassium perchlorate content is between 20% to 70%,which counted in the content of potassium element is between 4% to 23%. Concept of this method： considering the weight of the sample is 400rag,constant volume is 1L and the concentration of potassium is between 10 mg/L to 90 mg/L in sample solution, the determination scope of the method for the potassium content would be between 1% to 23%.Further experiments proved that the fitting correlation coefficient of potassium calibration curve is 0.9997 or higher, recovery is 89.15%-100.23%.The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.展开更多
Inductively coupled plasma optical emission spectrometric approach（ICP-OES）is used to determine the magnesium and aluminum content as principal components in pyrotechnic compositions used for fireworks and firecrack...Inductively coupled plasma optical emission spectrometric approach（ICP-OES）is used to determine the magnesium and aluminum content as principal components in pyrotechnic compositions used for fireworks and firecrackers. Elements of magnesium and aluminum are commonly found in aluminum powder or magnesium-aluminum alloy powder in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that the magnesium content in pyrotechnics is between 8% to 30% and the aluminum content is between 8% to 35%（roughly）.Concept of this method： suppose the weight of the sample is 400rag,constant volume is IL and the concentlation of magnesium and aluminum is between 12mg/L to 160mg/L in sample solution, the determination scope of the method for magnesium and aluminum content would be between 3% to 40%.Further experiments proved that the fitting correlation coefficient of the magnesium calibration curve is 0.9999 or higher, recovery is 101.01% -101.96%.The fitting correlation coefficient of the aluminum calibration curve is 0.9999 or higher, recovery is 99.36%-103.07%. The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.展开更多
[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.Th...[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.展开更多
目的:建立一测多评法(QAMS)同时测定蒙古族成药每粒清热八味胶囊中胆红素、羟基红花黄色素A、芦丁、胡黄连苷Ⅱ、胡黄连苷Ⅰ、肉桂酸、槲皮素、紫堇灵、山柰素的质量。方法:采用高效液相色谱法,安捷伦Zorbax Eclipse Plus C18色谱柱(250...目的:建立一测多评法(QAMS)同时测定蒙古族成药每粒清热八味胶囊中胆红素、羟基红花黄色素A、芦丁、胡黄连苷Ⅱ、胡黄连苷Ⅰ、肉桂酸、槲皮素、紫堇灵、山柰素的质量。方法:采用高效液相色谱法,安捷伦Zorbax Eclipse Plus C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.4%磷酸水溶液为流动相,梯度洗脱,流速为1.0 m L·min^(–1),检测波长分别为275、370、403 nm,柱温为35℃。以羟基红花黄色素A和胡黄连苷Ⅰ为双标化合物,使用双标线性校正法(LCTRC)预测tR。以胡黄连苷Ⅰ为内参物,建立与其他8个成分的相对校正因子,并计算每粒中9个待测成分的质量,实现一测多评。同时与外标法进行比较,验证QAMS的准确性和可行性。结果:9个成分在各自质量浓度范围内的线性关系良好(r≥0.9990),平均加样回收率为93.0%~99.8%,RSD为0.23%~1.53%;建立的QAMS可用于测定每粒清热八味胶囊中9个指标成分的质量,并对6批清热八味胶囊进行测定,其计算值与测定值的差异无统计学意义。结论:QAMS测定清热八味胶囊中9个成分准确且可行,可用于清热八味胶囊的质量控制。展开更多
基金Supported by Provincial University Scientific Research Platform Team Project of Guizhou Provincial Department of Education(Qianjiaoji[2022]No.010).
文摘[Objectives]To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine.[Methods]High performance liquid chromatography(HPLC)with ShimNex CS C 18 as the column;column temperature:35℃;wavelength:270 nm;methanol-0.1%phosphoric acid solution as the mobile phase with gradient elution.[Results]The 12 components of the retention of Longqing Capsule showed good linearity within the investigated range(r≥0.9995),with the average spiked recoveries of 97.83%-100.52%and the RSD of 0.9%-2.1%.[Conclusions]The method is exclusive,sensitive,reproducible,simple and easy to use,and can provide a reference for the construction of the quality standard and control system of Longqing Capsule based on the theory of traditional Chinese medicine.
文摘Inductively coupled plasma optical emission spectrometric approach（1CP-OES） is used to determine the potassium content as principal component in pyrotechnic compositions used for fireworks and firecrackers. Element of potassium is conunonly found in potassium nitrate and potassium perchlorate in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that potassium nitrate content in pyrotechnics is between 10% to 60% and the potassium perchlorate content is between 20% to 70%,which counted in the content of potassium element is between 4% to 23%. Concept of this method： considering the weight of the sample is 400rag,constant volume is 1L and the concentration of potassium is between 10 mg/L to 90 mg/L in sample solution, the determination scope of the method for the potassium content would be between 1% to 23%.Further experiments proved that the fitting correlation coefficient of potassium calibration curve is 0.9997 or higher, recovery is 89.15%-100.23%.The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.
文摘Inductively coupled plasma optical emission spectrometric approach（ICP-OES）is used to determine the magnesium and aluminum content as principal components in pyrotechnic compositions used for fireworks and firecrackers. Elements of magnesium and aluminum are commonly found in aluminum powder or magnesium-aluminum alloy powder in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that the magnesium content in pyrotechnics is between 8% to 30% and the aluminum content is between 8% to 35%（roughly）.Concept of this method： suppose the weight of the sample is 400rag,constant volume is IL and the concentlation of magnesium and aluminum is between 12mg/L to 160mg/L in sample solution, the determination scope of the method for magnesium and aluminum content would be between 3% to 40%.Further experiments proved that the fitting correlation coefficient of the magnesium calibration curve is 0.9999 or higher, recovery is 101.01% -101.96%.The fitting correlation coefficient of the aluminum calibration curve is 0.9999 or higher, recovery is 99.36%-103.07%. The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.
基金Collaborative Innovation in Tibetan Medicine(2019XTC013)Open Laboratory of Key Laboratory of Tibetan Medicine Basic Education(ZYYJC-19-01).
文摘[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.
文摘目的:建立一测多评法(QAMS)同时测定蒙古族成药每粒清热八味胶囊中胆红素、羟基红花黄色素A、芦丁、胡黄连苷Ⅱ、胡黄连苷Ⅰ、肉桂酸、槲皮素、紫堇灵、山柰素的质量。方法:采用高效液相色谱法,安捷伦Zorbax Eclipse Plus C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.4%磷酸水溶液为流动相,梯度洗脱,流速为1.0 m L·min^(–1),检测波长分别为275、370、403 nm,柱温为35℃。以羟基红花黄色素A和胡黄连苷Ⅰ为双标化合物,使用双标线性校正法(LCTRC)预测tR。以胡黄连苷Ⅰ为内参物,建立与其他8个成分的相对校正因子,并计算每粒中9个待测成分的质量,实现一测多评。同时与外标法进行比较,验证QAMS的准确性和可行性。结果:9个成分在各自质量浓度范围内的线性关系良好(r≥0.9990),平均加样回收率为93.0%~99.8%,RSD为0.23%~1.53%;建立的QAMS可用于测定每粒清热八味胶囊中9个指标成分的质量,并对6批清热八味胶囊进行测定,其计算值与测定值的差异无统计学意义。结论:QAMS测定清热八味胶囊中9个成分准确且可行,可用于清热八味胶囊的质量控制。
