Endothelial cells (TEC_3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × 10~6 smooth muscle cells (...Endothelial cells (TEC_3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × 10~6 smooth muscle cells (SMCs) obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid (PGA) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the cell-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6～8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC_3 cells (endothelial cells differentiated from mouse ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC-PGA construct could form a tubular structure in 6～8 weeks and PGA fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from mouse ES cells can serve as seed cells for endothelium lining in tissue engineered blood vessels.展开更多
Applying man-machine-environment system engineering(MMESE)in vessels is a method to improve the effectiveness of the interaction between equipment, environment, and humans for the purpose of advancing operating effici...Applying man-machine-environment system engineering(MMESE)in vessels is a method to improve the effectiveness of the interaction between equipment, environment, and humans for the purpose of advancing operating efficiency, performance, safety, and habitability of a vessel and its subsystems. In the following research, the life cycle of vessels was divided into 9 phases, and 15 research subjects were also identified from among these phases. The 15 subjects were systemized, and then the man-machine-environment engineering system application model for vessels was developed using the ICAM definition method 0 (IDEF0), which is a systematical modeling method. This system model bridges the gap between the data and information flow of every two associated subjects with the major basic research methods and approaches included, which brings the formerly relatively independent subjects together as a whole. The application of this systematic model should facilitate the application of man-machine-environment system engineering in vessels, especially at the conceptual and embodiment design phases. The managers and designers can deal with detailed tasks quickly and efficiently while reducing repetitive work.展开更多
Tubular microfibers have recently attracted extensive interest for applications in tissue engineering.However,the fabrication of tubular fibers with intricate hierarchical structures remains a major challenge.Here,we ...Tubular microfibers have recently attracted extensive interest for applications in tissue engineering.However,the fabrication of tubular fibers with intricate hierarchical structures remains a major challenge.Here,we present a novel one-step microfluidic spinning method to generate bio-inspired screwed conduits(BSCs).Based on the microfluidic rope-coiling effect,a viscous hydrogel precursor is first curved into a helix stream in the channel,and then consecutively packed as a hollow structured stream and gelated into a screwed conduit(SC)via ionic and covalent crosslinking.By taking advantage of the excellent fluid-controlling ability of microfluidics,various tubes with diverse structures are fabricated via simple control over fluid velocities and multiple microfluidic device designs.The perfusability and permeability results,as well as the encapsulation and culture of human umbilical vein endothelial cells(HUVECs),human pulmonary alveolar epithelial cells(HPAs),and myogenic cells(C2C12),demonstrate that these SCs have good perfusability and permeability and the ability to induce the formation of functional biostructures.These features support the uniqueness and potential applications of these BSCs as biomimetic blood vessels and bronchiole tissues in combination with tissue microstructures,with likely application possibilities in biomedical engineering.展开更多
背景:H型血管(CD31hiEmcnhi)因独特的功能为深入理解血管介导的骨代谢调控机制提供了新的视角和切入点,引发了骨科学研究范式从“骨本位”向“骨-血管整合”的重要转变。目的:基于H型血管领域的文献数据进行可视化分析,探讨该领域的研...背景:H型血管(CD31hiEmcnhi)因独特的功能为深入理解血管介导的骨代谢调控机制提供了新的视角和切入点,引发了骨科学研究范式从“骨本位”向“骨-血管整合”的重要转变。目的:基于H型血管领域的文献数据进行可视化分析,探讨该领域的研究热点和前沿趋势。方法:系统检索CNKI和Web of Science核心合集中与H型血管相关的中英文文献,文献检索时限为2014-2024年。采用CiteSpace 6.2.R4信息可视化软件对文献的发表国家、作者、机构、关键词、时间线视图等进行可视化分析。结果与结论:①共纳入中文文献59篇、英文文献185篇。自2014年提出H型血管概念以来,相关研究文献数量逐年增加,中国在该领域的研究处于领先地位,主要研究机构包括南方医科大学、四川大学和上海交通大学等。关键词分析揭示,当前研究热点主要集中在H型血管的生成与调控机制、H型血管在骨生成和骨重塑过程中的作用以及H型血管与骨质代谢相关疾病的关系;此外,出现“诱导膜”“软骨修复”“中医药”等新兴关键词,表明研究正在向多样化和多学科交叉的方向发展。②H型血管在骨骼疾病的发生发展中发挥着关键作用,相关研究对深入理解骨组织的生理与病理过程具有重要意义。未来应进一步探讨H型血管在不同疾病背景下的具体作用机制,推动基础研究成果的临床转化,为骨骼疾病的预防和治疗提供新的思路与策略。展开更多
基金supported by the national“973”tissue engineering project of China(G1999054300)Shanghai Science and Technology Development Foundation(03DJ14021)
文摘Endothelial cells (TEC_3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × 10~6 smooth muscle cells (SMCs) obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid (PGA) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the cell-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6～8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC_3 cells (endothelial cells differentiated from mouse ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC-PGA construct could form a tubular structure in 6～8 weeks and PGA fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from mouse ES cells can serve as seed cells for endothelium lining in tissue engineered blood vessels.
基金Supported by the Fundamental Research Program of CSTIND under Grant No.GF2007004Harbin Engineering University Central Foundation under Grant No.HEUCF100718
文摘Applying man-machine-environment system engineering(MMESE)in vessels is a method to improve the effectiveness of the interaction between equipment, environment, and humans for the purpose of advancing operating efficiency, performance, safety, and habitability of a vessel and its subsystems. In the following research, the life cycle of vessels was divided into 9 phases, and 15 research subjects were also identified from among these phases. The 15 subjects were systemized, and then the man-machine-environment engineering system application model for vessels was developed using the ICAM definition method 0 (IDEF0), which is a systematical modeling method. This system model bridges the gap between the data and information flow of every two associated subjects with the major basic research methods and approaches included, which brings the formerly relatively independent subjects together as a whole. The application of this systematic model should facilitate the application of man-machine-environment system engineering in vessels, especially at the conceptual and embodiment design phases. The managers and designers can deal with detailed tasks quickly and efficiently while reducing repetitive work.
基金supported by the National Key Research and Development Program of China(2020YFA0710800)the Key Program of National Natural Science Foundation of China(81930043and 82330055)the National Natural Science Foundation of China(82101184).
文摘Tubular microfibers have recently attracted extensive interest for applications in tissue engineering.However,the fabrication of tubular fibers with intricate hierarchical structures remains a major challenge.Here,we present a novel one-step microfluidic spinning method to generate bio-inspired screwed conduits(BSCs).Based on the microfluidic rope-coiling effect,a viscous hydrogel precursor is first curved into a helix stream in the channel,and then consecutively packed as a hollow structured stream and gelated into a screwed conduit(SC)via ionic and covalent crosslinking.By taking advantage of the excellent fluid-controlling ability of microfluidics,various tubes with diverse structures are fabricated via simple control over fluid velocities and multiple microfluidic device designs.The perfusability and permeability results,as well as the encapsulation and culture of human umbilical vein endothelial cells(HUVECs),human pulmonary alveolar epithelial cells(HPAs),and myogenic cells(C2C12),demonstrate that these SCs have good perfusability and permeability and the ability to induce the formation of functional biostructures.These features support the uniqueness and potential applications of these BSCs as biomimetic blood vessels and bronchiole tissues in combination with tissue microstructures,with likely application possibilities in biomedical engineering.
文摘背景:H型血管(CD31hiEmcnhi)因独特的功能为深入理解血管介导的骨代谢调控机制提供了新的视角和切入点,引发了骨科学研究范式从“骨本位”向“骨-血管整合”的重要转变。目的:基于H型血管领域的文献数据进行可视化分析,探讨该领域的研究热点和前沿趋势。方法:系统检索CNKI和Web of Science核心合集中与H型血管相关的中英文文献,文献检索时限为2014-2024年。采用CiteSpace 6.2.R4信息可视化软件对文献的发表国家、作者、机构、关键词、时间线视图等进行可视化分析。结果与结论:①共纳入中文文献59篇、英文文献185篇。自2014年提出H型血管概念以来,相关研究文献数量逐年增加,中国在该领域的研究处于领先地位,主要研究机构包括南方医科大学、四川大学和上海交通大学等。关键词分析揭示,当前研究热点主要集中在H型血管的生成与调控机制、H型血管在骨生成和骨重塑过程中的作用以及H型血管与骨质代谢相关疾病的关系;此外,出现“诱导膜”“软骨修复”“中医药”等新兴关键词,表明研究正在向多样化和多学科交叉的方向发展。②H型血管在骨骼疾病的发生发展中发挥着关键作用,相关研究对深入理解骨组织的生理与病理过程具有重要意义。未来应进一步探讨H型血管在不同疾病背景下的具体作用机制,推动基础研究成果的临床转化,为骨骼疾病的预防和治疗提供新的思路与策略。
