Fourier ptychographic microscopy(FPM)is an innovative computational microscopy approach that enables high-throughput imaging with high resolution,wide field of view,and quantitative phase imaging(QPI)by simultaneously...Fourier ptychographic microscopy(FPM)is an innovative computational microscopy approach that enables high-throughput imaging with high resolution,wide field of view,and quantitative phase imaging(QPI)by simultaneously capturing bright-field and dark-field images.However,effectively utilizing dark-field intensity images,including both normally exposed and overexposed data,which contain valuable high-angle illumination information,remains a complex challenge.Successfully extracting and applying this information could significantly enhance phase reconstruction,benefiting processes such as virtual staining and QPI imaging.To address this,we introduce a multi-exposure image fusion(MEIF)framework that optimizes dark-field information by incorporating it into the FPM preprocessing workflow.MEIF increases the data available for reconstruction without requiring changes to the optical setup.We evaluate the framework using both feature-domain and traditional FPM,demonstrating that it achieves substantial improvements in intensity resolution and phase information for biological samples that exceed the performance of conventional high dynamic range(HDR)methods.This image preprocessing-based information-maximization strategy fully leverages existing datasets and offers promising potential to drive advancements in fields such as microscopy,remote sensing,and crystallography.展开更多
The traditional EnFCM(Enhanced fuzzy C-means)algorithm only considers the grey-scale features in image segmentation,resulting in less than satisfactory results when the algorithm is used for remote sensing woodland im...The traditional EnFCM(Enhanced fuzzy C-means)algorithm only considers the grey-scale features in image segmentation,resulting in less than satisfactory results when the algorithm is used for remote sensing woodland image segmentation and extraction.An EnFCM remote sensing forest land extraction method based on PCA multi-feature fusion was proposed.Firstly,histogram equalization was applied to improve the image contrast.Secondly,the texture and edge features of the image were extracted,and a multi-feature fused pixel image was generated using the PCA technique.Moreover,the fused feature was used as a feature constraint to measure the difference of pixels instead of a single grey-scale feature.Finally,an improved feature distance metric calculated the similarity between the pixel points and the cluster center to complete the cluster segmentation.The experimental results showed that the error was between 1.5%and 4.0%compared with the forested area counted by experts’hand-drawing,which could obtain a high accuracy segmentation and extraction result.展开更多
In recent years,many medical image fusion methods had been exploited to derive useful information from multimodality medical image data,but,not an appropriate fusion algorithm for anatomical and functional medical ima...In recent years,many medical image fusion methods had been exploited to derive useful information from multimodality medical image data,but,not an appropriate fusion algorithm for anatomical and functional medical images.In this paper,the traditional method of wavelet fusion is improved and a new fusion algorithm of anatomical and functional medical images,in which high-frequency and low-frequency coefficients are studied respectively.When choosing high-frequency coefficients,the global gradient of each sub-image is calculated to realize adaptive fusion,so that the fused image can reserve the functional information;while choosing the low coefficients is based on the analysis of the neighborbood region energy,so that the fused image can reserve the anatomical image's edge and texture feature.Experimental results and the quality evaluation parameters show that the improved fusion algorithm can enhance the edge and texture feature and retain the function information and anatomical information effectively.展开更多
Different fusion algorithm has its own advantages and limitations,so it is very difficult to simply evaluate the good points and bad points of the fusion algorithm. Whether an algorithm was selected to fuse object ima...Different fusion algorithm has its own advantages and limitations,so it is very difficult to simply evaluate the good points and bad points of the fusion algorithm. Whether an algorithm was selected to fuse object images was also depended upon the sensor types and special research purposes. Firstly,five fusion methods,i. e. IHS,Brovey,PCA,SFIM and Gram-Schmidt,were briefly described in the paper. And then visual judgment and quantitative statistical parameters were used to assess the five algorithms. Finally,in order to determine which one is the best suitable fusion method for land cover classification of IKONOS image,the maximum likelihood classification( MLC) was applied using the above five fusion images. The results showed that the fusion effect of SFIM transform and Gram-Schmidt transform were better than the other three image fusion methods in spatial details improvement and spectral information fidelity,and Gram-Schmidt technique was superior to SFIM transform in the aspect of expressing image details. The classification accuracy of the fused image using Gram-Schmidt and SFIM algorithms was higher than that of the other three image fusion methods,and the overall accuracy was greater than 98%. The IHS-fused image classification accuracy was the lowest,the overall accuracy and kappa coefficient were 83. 14% and 0. 76,respectively. Thus the IKONOS fusion images obtained by the Gram-Schmidt and SFIM were better for improving the land cover classification accuracy.展开更多
Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imag...Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imaging processing. It could match the images and improve the confidence and spatial resolution of the images. Using two algorithms, double thresholds algorithm and denoising algorithm based on wavelet transform,the fluorescence image and transmission image of a Cell were merged into a composite image. Results and Conclusion The position of fluorescence and the structure of cell can be displyed in the composite image. The signal-to-noise ratio of the exultant image is improved to a large extent. The algorithms are not only useful to investigate the fluorescence and transmission images, but also suitable to observing two or more fluoascent label proes in a single cell.展开更多
Image fusion technology is the basis of computer vision task,but information is easily affected by noise during transmission.In this paper,an Improved Pigeon-Inspired Optimization(IPIO)is proposed,and used for multi-f...Image fusion technology is the basis of computer vision task,but information is easily affected by noise during transmission.In this paper,an Improved Pigeon-Inspired Optimization(IPIO)is proposed,and used for multi-focus noisy image fusion by combining with the boundary handling of the convolutional sparse representation.By two-scale image decomposition,the input image is decomposed into base layer and detail layer.For the base layer,IPIO algorithm is used to obtain the optimized weights for fusion,whose value range is gained by fusing the edge information.Besides,the global information entropy is used as the fitness index of the IPIO,which has high efficiency especially for discrete optimization problems.For the detail layer,the fusion of its coefficients is completed by performing boundary processing when solving the convolution sparse representation in the frequency domain.The sum of the above base and detail layers is as the final fused image.Experimental results show that the proposed algorithm has a better fusion effect compared with the recent algorithms.展开更多
This paper introduces the image fusion approach of multi-resolutionanalysis-based intensity modulation (MRAIM) to produce the high-resolution multi-spectral imagesfrom high-resolution panchromatic image and low-resolu...This paper introduces the image fusion approach of multi-resolutionanalysis-based intensity modulation (MRAIM) to produce the high-resolution multi-spectral imagesfrom high-resolution panchromatic image and low-resolution multi-spectral images for navigationinformation infrastructure. The mathematical model of image fusion is derived according to theprinciple of remote sensing image formation. It shows that the pixel values of a high-resolutionmulti-spectral images are determined by the pixel values of the approximation of a high-resolutionpanchromatic image at the resolution level of low-resolution multi-spectral images, and in the pixelvalae computation the M-band wavelet theory and the a trous algorithm are then used. In order toevaluate the MRAIM approach, an experiment has been carried out on the basis of the IKONOS 1 mpanchromatic image and 4 m multi-spectral images. The result demonstrates that MRAIM image fusionapproach gives promising fusion results and it can be used to produce the high-resolution remotesensing images required for navigation information infrastructures.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.12104500)the Key Research and Development Projects of Shaanxi Province of China(Grant No.2023-YBSF-263).
文摘Fourier ptychographic microscopy(FPM)is an innovative computational microscopy approach that enables high-throughput imaging with high resolution,wide field of view,and quantitative phase imaging(QPI)by simultaneously capturing bright-field and dark-field images.However,effectively utilizing dark-field intensity images,including both normally exposed and overexposed data,which contain valuable high-angle illumination information,remains a complex challenge.Successfully extracting and applying this information could significantly enhance phase reconstruction,benefiting processes such as virtual staining and QPI imaging.To address this,we introduce a multi-exposure image fusion(MEIF)framework that optimizes dark-field information by incorporating it into the FPM preprocessing workflow.MEIF increases the data available for reconstruction without requiring changes to the optical setup.We evaluate the framework using both feature-domain and traditional FPM,demonstrating that it achieves substantial improvements in intensity resolution and phase information for biological samples that exceed the performance of conventional high dynamic range(HDR)methods.This image preprocessing-based information-maximization strategy fully leverages existing datasets and offers promising potential to drive advancements in fields such as microscopy,remote sensing,and crystallography.
基金supported by National Natural Science Foundation of China(No.61761027)Gansu Young Doctor’s Fund for Higher Education Institutions(No.2021QB-053)。
文摘The traditional EnFCM(Enhanced fuzzy C-means)algorithm only considers the grey-scale features in image segmentation,resulting in less than satisfactory results when the algorithm is used for remote sensing woodland image segmentation and extraction.An EnFCM remote sensing forest land extraction method based on PCA multi-feature fusion was proposed.Firstly,histogram equalization was applied to improve the image contrast.Secondly,the texture and edge features of the image were extracted,and a multi-feature fused pixel image was generated using the PCA technique.Moreover,the fused feature was used as a feature constraint to measure the difference of pixels instead of a single grey-scale feature.Finally,an improved feature distance metric calculated the similarity between the pixel points and the cluster center to complete the cluster segmentation.The experimental results showed that the error was between 1.5%and 4.0%compared with the forested area counted by experts’hand-drawing,which could obtain a high accuracy segmentation and extraction result.
基金The National High Technology Research and Development Program of China(‘863’Program)grant number:2007AA02Z4A9+1 种基金National Natural Science Foundation of Chinagrant number:30671997
文摘In recent years,many medical image fusion methods had been exploited to derive useful information from multimodality medical image data,but,not an appropriate fusion algorithm for anatomical and functional medical images.In this paper,the traditional method of wavelet fusion is improved and a new fusion algorithm of anatomical and functional medical images,in which high-frequency and low-frequency coefficients are studied respectively.When choosing high-frequency coefficients,the global gradient of each sub-image is calculated to realize adaptive fusion,so that the fused image can reserve the functional information;while choosing the low coefficients is based on the analysis of the neighborbood region energy,so that the fused image can reserve the anatomical image's edge and texture feature.Experimental results and the quality evaluation parameters show that the improved fusion algorithm can enhance the edge and texture feature and retain the function information and anatomical information effectively.
基金Supported by Chinese National Natural Science Foundation(51208016)Beijing Natural Science Foundation(8122008)Beijing Education Commission Fund(KM201310005023)
文摘Different fusion algorithm has its own advantages and limitations,so it is very difficult to simply evaluate the good points and bad points of the fusion algorithm. Whether an algorithm was selected to fuse object images was also depended upon the sensor types and special research purposes. Firstly,five fusion methods,i. e. IHS,Brovey,PCA,SFIM and Gram-Schmidt,were briefly described in the paper. And then visual judgment and quantitative statistical parameters were used to assess the five algorithms. Finally,in order to determine which one is the best suitable fusion method for land cover classification of IKONOS image,the maximum likelihood classification( MLC) was applied using the above five fusion images. The results showed that the fusion effect of SFIM transform and Gram-Schmidt transform were better than the other three image fusion methods in spatial details improvement and spectral information fidelity,and Gram-Schmidt technique was superior to SFIM transform in the aspect of expressing image details. The classification accuracy of the fused image using Gram-Schmidt and SFIM algorithms was higher than that of the other three image fusion methods,and the overall accuracy was greater than 98%. The IHS-fused image classification accuracy was the lowest,the overall accuracy and kappa coefficient were 83. 14% and 0. 76,respectively. Thus the IKONOS fusion images obtained by the Gram-Schmidt and SFIM were better for improving the land cover classification accuracy.
文摘Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imaging processing. It could match the images and improve the confidence and spatial resolution of the images. Using two algorithms, double thresholds algorithm and denoising algorithm based on wavelet transform,the fluorescence image and transmission image of a Cell were merged into a composite image. Results and Conclusion The position of fluorescence and the structure of cell can be displyed in the composite image. The signal-to-noise ratio of the exultant image is improved to a large extent. The algorithms are not only useful to investigate the fluorescence and transmission images, but also suitable to observing two or more fluoascent label proes in a single cell.
基金supported in part by National Key Research and Development Program of China(2018YFB0804202,2018YFB0804203)Regional Joint Fund of NSFC(U19A2057)+1 种基金National Natural Science Foundation of China(61876070)Jilin Province Science and Technology Development Plan Project(20190303134SF).
文摘Image fusion technology is the basis of computer vision task,but information is easily affected by noise during transmission.In this paper,an Improved Pigeon-Inspired Optimization(IPIO)is proposed,and used for multi-focus noisy image fusion by combining with the boundary handling of the convolutional sparse representation.By two-scale image decomposition,the input image is decomposed into base layer and detail layer.For the base layer,IPIO algorithm is used to obtain the optimized weights for fusion,whose value range is gained by fusing the edge information.Besides,the global information entropy is used as the fitness index of the IPIO,which has high efficiency especially for discrete optimization problems.For the detail layer,the fusion of its coefficients is completed by performing boundary processing when solving the convolution sparse representation in the frequency domain.The sum of the above base and detail layers is as the final fused image.Experimental results show that the proposed algorithm has a better fusion effect compared with the recent algorithms.
文摘This paper introduces the image fusion approach of multi-resolutionanalysis-based intensity modulation (MRAIM) to produce the high-resolution multi-spectral imagesfrom high-resolution panchromatic image and low-resolution multi-spectral images for navigationinformation infrastructure. The mathematical model of image fusion is derived according to theprinciple of remote sensing image formation. It shows that the pixel values of a high-resolutionmulti-spectral images are determined by the pixel values of the approximation of a high-resolutionpanchromatic image at the resolution level of low-resolution multi-spectral images, and in the pixelvalae computation the M-band wavelet theory and the a trous algorithm are then used. In order toevaluate the MRAIM approach, an experiment has been carried out on the basis of the IKONOS 1 mpanchromatic image and 4 m multi-spectral images. The result demonstrates that MRAIM image fusionapproach gives promising fusion results and it can be used to produce the high-resolution remotesensing images required for navigation information infrastructures.
