Unexpected benefits to the catalytic performance of materials often originate from the presence of surface defects.Here,novel Dpenicillamine modified molybdenum oxide nanodots,with abundant oxygen vacancy defects,were...Unexpected benefits to the catalytic performance of materials often originate from the presence of surface defects.Here,novel Dpenicillamine modified molybdenum oxide nanodots,with abundant oxygen vacancy defects,were fabrication by a mild,simple,and cost-effective method.Ultraviolet–visible(UV–Vis)absorption spectra analysis showed that the nanodots had peroxidaselike and catalase-like activities.The reactive oxygen species were probed by electronic paramagnetic resonance technique and spectroscopic methods,demonstrating that the nanodots also had oxidase-like activity.Interestingly,the peroxidase-like activity of nanodots was synergistically enhanced in the presence of ferrous ions or ferric ions.Remarkably,less than nanomolar levels of ferrous ions were required to display this phenomenon,meaning Fenton reagent acted as leverage.Based on this,a sensitive colorimetric and fluorescent dual-mode sensor for alendronate sodium was developed.The linear ranges for colorimetric and fluorescence analysis were 0.2–2.5 and 0.2–2.0μM,with detection limits of 31.21 and 71.84 nM,correspondingly.The method has a simple large-scale material preparation process with higher sensitivity and shorter reaction time,which can inspire and enlighten the design of nanozyme sensors.展开更多
Bacterial infections,inflammatory responses,and tissue hypoxia are three critical characteristics of the infection-healing process.To shorten the duration of infection and inflammation,this study developed a microenvi...Bacterial infections,inflammatory responses,and tissue hypoxia are three critical characteristics of the infection-healing process.To shorten the duration of infection and inflammation,this study developed a microenvironmental adaptive nanozyme with photothermal-enhanced multi-enzyme-like activity to address the unique challenges associated with each stage of healing.This was achieved by anchoring platinum onto molybdenum disulfide nanozymes(Pt-MoS_(2)),which endowed them with photothermal conversion properties and biocatalytic activity.Additionally,the nanozymes generate reactive oxygen species in acidic microenvironments and consume glutathione during the early stages of infection,thus optimizing antibacterial performance.As the infection is controlled and the pH increases,the activities of oxidase-like and peroxidase-like enzymes decrease,while the catalase-like enzyme activity becomes more prominent.Pt-MoS_(2) mitigates the detrimental effects of excessive H2O2 and alleviates hypoxia through its peroxidase-like activity,thereby creating a favorable environment for wound healing.This approach has been validated in a subcutaneous abscess model infected with methicillin-resistant Staphylococcus aureus.Pt-MoS_(2) nanozymes present a novel strategy for subsequent antiinfective treatments.展开更多
基金National Natural Science Foundation of China(No.21603276)Fundamental Research Funds for the Central Universities(Nos.19CX02060A and 22CX03024A)the Natural Science Foundation of Shandong Province(No.ZR2022MB148).
文摘Unexpected benefits to the catalytic performance of materials often originate from the presence of surface defects.Here,novel Dpenicillamine modified molybdenum oxide nanodots,with abundant oxygen vacancy defects,were fabrication by a mild,simple,and cost-effective method.Ultraviolet–visible(UV–Vis)absorption spectra analysis showed that the nanodots had peroxidaselike and catalase-like activities.The reactive oxygen species were probed by electronic paramagnetic resonance technique and spectroscopic methods,demonstrating that the nanodots also had oxidase-like activity.Interestingly,the peroxidase-like activity of nanodots was synergistically enhanced in the presence of ferrous ions or ferric ions.Remarkably,less than nanomolar levels of ferrous ions were required to display this phenomenon,meaning Fenton reagent acted as leverage.Based on this,a sensitive colorimetric and fluorescent dual-mode sensor for alendronate sodium was developed.The linear ranges for colorimetric and fluorescence analysis were 0.2–2.5 and 0.2–2.0μM,with detection limits of 31.21 and 71.84 nM,correspondingly.The method has a simple large-scale material preparation process with higher sensitivity and shorter reaction time,which can inspire and enlighten the design of nanozyme sensors.
基金supported by the National Natural Science Foundation of China(No.22207088)National Clinical Key Specialty Internal Fund of Quzhou Hospital Affiliated of Wenzhou Medical University(Internal Research Project)(No.GJZK-01)Leading Talents of Nankong Elite(No.KYQD2024-04).
文摘Bacterial infections,inflammatory responses,and tissue hypoxia are three critical characteristics of the infection-healing process.To shorten the duration of infection and inflammation,this study developed a microenvironmental adaptive nanozyme with photothermal-enhanced multi-enzyme-like activity to address the unique challenges associated with each stage of healing.This was achieved by anchoring platinum onto molybdenum disulfide nanozymes(Pt-MoS_(2)),which endowed them with photothermal conversion properties and biocatalytic activity.Additionally,the nanozymes generate reactive oxygen species in acidic microenvironments and consume glutathione during the early stages of infection,thus optimizing antibacterial performance.As the infection is controlled and the pH increases,the activities of oxidase-like and peroxidase-like enzymes decrease,while the catalase-like enzyme activity becomes more prominent.Pt-MoS_(2) mitigates the detrimental effects of excessive H2O2 and alleviates hypoxia through its peroxidase-like activity,thereby creating a favorable environment for wound healing.This approach has been validated in a subcutaneous abscess model infected with methicillin-resistant Staphylococcus aureus.Pt-MoS_(2) nanozymes present a novel strategy for subsequent antiinfective treatments.
