Aim： To develop a high-throughput multiplex, fast and simple assay to scan azoospermia factor （AZF） region microdeletions on the Y chromosome and establish the prevalence of Y chromosomal microdeletions in Chinese ...Aim： To develop a high-throughput multiplex, fast and simple assay to scan azoospermia factor （AZF） region microdeletions on the Y chromosome and establish the prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia. Methods： In total, 178 infertile patients with azoospermia （nonobstructed）, 134 infertile patients with oligozoospermia as well as 40 fertile man controls were included in the present study. The samples were screened for AZF microdeletion using optimized multi-analyte suspension array （MASA） technology. Results： Of the 312 patients, 36 （11.5%） were found to have deletions in the AZF region. The rnicrodeletion frequency was 14% （25/178） in the azoospermia group and 8.2% （11/134） in the oligospermia group. Among 36 patients with microdeletions, 19 had deletions in the AZFc region, seven had deletions in AZFa and six had deletions in AZFb. In addition, four patients had both AZFb and AZFc deletions. No deletion in the AZF region was found in the 40 fertile controls. Conclusion： There is a high prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia. The MASA technology, which has been established in the present study, provides a sensitive and high-throughput method for detecting the deletion of the Y chromosome. And the results suggest that genetic screening should be advised to infertile men before starting assisted reproductive treatments.展开更多
Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An ...Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO_(2) thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool.展开更多
In this paper,dual-stabilizer-capped CdTe quantum dots were used as modulated photoluminescence(PL)sensors for the subpicomolar level detection of copper(II)(Cu^(2+))and mercury(II)(Hg^(2+))ions in aqueous solution fo...In this paper,dual-stabilizer-capped CdTe quantum dots were used as modulated photoluminescence(PL)sensors for the subpicomolar level detection of copper(II)(Cu^(2+))and mercury(II)(Hg^(2+))ions in aqueous solution for the first time.The dual-stabilizer-capped CdTe quantum dots were synthesized using mercaptopropionic acid(MPA)and sodium hexametaphosphate(SHMP)as surface-modified ligands via a convenient hydrothermal process.The researches showed a low interference response of the MPASHMP-capped CdTe quantum dots towards other metal ions.The highly efficient PL quenching ability in the presence of Hg^(2+)or Cu^(2+)ions due to the formed nonfluorescent metal complexes via robust Hg^(2+)–O interaction with the carboxy oxygen elements of surface ligands of MPA,and on the basis of the competitive binding of the mercapto groups of the MPA between the CdTe quantum dots and the Cu^(2+)ions,respectively,which allowed the analysis of Hg^(2+)or Cu^(2+)ions down to the picomolar levels.Under optimal conditions,the response of the MPA-SHMP-capped CdTe quantum dot PL intensity is linearly proportional to the Cu^(2+)and Hg^(2+)ion concentration ranging from 0.1 to 1000 and 0.3 to 1000 nM with a detection limit of 41.6 and 97.0 pM,respectively.The diagnostic capability and potential in practical applications of this method have been demonstrated by detecting Cu^(2+)and Hg^(2+)ions in environmental water samples.展开更多
文摘Aim： To develop a high-throughput multiplex, fast and simple assay to scan azoospermia factor （AZF） region microdeletions on the Y chromosome and establish the prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia. Methods： In total, 178 infertile patients with azoospermia （nonobstructed）, 134 infertile patients with oligozoospermia as well as 40 fertile man controls were included in the present study. The samples were screened for AZF microdeletion using optimized multi-analyte suspension array （MASA） technology. Results： Of the 312 patients, 36 （11.5%） were found to have deletions in the AZF region. The rnicrodeletion frequency was 14% （25/178） in the azoospermia group and 8.2% （11/134） in the oligospermia group. Among 36 patients with microdeletions, 19 had deletions in the AZFc region, seven had deletions in AZFa and six had deletions in AZFb. In addition, four patients had both AZFb and AZFc deletions. No deletion in the AZF region was found in the 40 fertile controls. Conclusion： There is a high prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia. The MASA technology, which has been established in the present study, provides a sensitive and high-throughput method for detecting the deletion of the Y chromosome. And the results suggest that genetic screening should be advised to infertile men before starting assisted reproductive treatments.
基金financial support from the Spanish Agencia Estatal de Investigación (AEI) through project PID2023-149895OB-I00a predoctoral research grant from the Public University of Navarrafinancial support under the National Recovery and Resilience Plan (NRRP),Mission 4,Component 2,Investment 1.1,Call for tender No.1409 published on 14.9.2022 by the Italian Ministry of University and Research (MUR),funded by the European Union–NextGenerationEU–Project Title‘‘Fiber optics sensors as a platform for cancer diagnosis and in vitro model testing”–CUP B53D23024170001-Grant Assignment Decree No.1383 adopted on 01/09/2023 by the Italian MUR.
文摘Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO_(2) thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool.
基金the National Natural Science Foundation of China(21575022,21535003),the National High Technology Research and Development Program(“863”Program)of China(2015AA020502)the Fundamental Research Funds for the Central Universities,Qing Lan Project and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.And we greatly appreciate the support of the National Natural Science Foundation of China(21575123,21675139,21705140)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20170474)the Industry-University-Research Cooperative Innovation Foundation of Jiangsu Province(BY2015057-17).
文摘In this paper,dual-stabilizer-capped CdTe quantum dots were used as modulated photoluminescence(PL)sensors for the subpicomolar level detection of copper(II)(Cu^(2+))and mercury(II)(Hg^(2+))ions in aqueous solution for the first time.The dual-stabilizer-capped CdTe quantum dots were synthesized using mercaptopropionic acid(MPA)and sodium hexametaphosphate(SHMP)as surface-modified ligands via a convenient hydrothermal process.The researches showed a low interference response of the MPASHMP-capped CdTe quantum dots towards other metal ions.The highly efficient PL quenching ability in the presence of Hg^(2+)or Cu^(2+)ions due to the formed nonfluorescent metal complexes via robust Hg^(2+)–O interaction with the carboxy oxygen elements of surface ligands of MPA,and on the basis of the competitive binding of the mercapto groups of the MPA between the CdTe quantum dots and the Cu^(2+)ions,respectively,which allowed the analysis of Hg^(2+)or Cu^(2+)ions down to the picomolar levels.Under optimal conditions,the response of the MPA-SHMP-capped CdTe quantum dot PL intensity is linearly proportional to the Cu^(2+)and Hg^(2+)ion concentration ranging from 0.1 to 1000 and 0.3 to 1000 nM with a detection limit of 41.6 and 97.0 pM,respectively.The diagnostic capability and potential in practical applications of this method have been demonstrated by detecting Cu^(2+)and Hg^(2+)ions in environmental water samples.
