Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood o...Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identifi ed. The genetic diversity was calculated to be 0.9691, and the genetic identity was calculated to be 0.0406. Conclusion There are some particul ar polymorphism sites in Chinese YUGU ethnic group. The results suggest that seq uence polymorphism from np16091-16418 in human mitochondrial DNA can be used as a biological marker for forensic identity.展开更多
Objective: To investigate the mitochondrial DNA sequence polymorphism in Chinese Dongxiang ethnic group and to provide basic data used in ethnic origin investigation and forensic purpose. Methods: Genomic DNA was extr...Objective: To investigate the mitochondrial DNA sequence polymorphism in Chinese Dongxiang ethnic group and to provide basic data used in ethnic origin investigation and forensic purpose. Methods: Genomic DNA was extracted from the whole blood of 100 unrelated individuals of Chinese Dongxiang ethnic group by standard Chelex-100 method. The sequence polymorphism was determined by PCR amplification and direct sequencing. Results: Eighty-two polymorphic sites were identified in mtDNA D-loop region 16 091 - 16 418 np, and 88 haplotypes were found. The genetic diversity was calculated to he 0.9969, and the genetic identity was 0.013 2. Conclusion: There are some particular polymorphic sites in Chinese Dongxiang ethnic group, and these sites provide an important basis to investigate the origin of Dongxiang and the relationship between Dongxiang and other ethnic groups. The result also suggested that sequence polymorphism from 16 091 -16 418 np in human mitochondrial DNA control region can be an useful tool for forensic identity.展开更多
The complete mitochondrial DNA D-loop sequences from 10 stud Brahman cattle were sequenced and analyzed. The results showed that the genetic diversity of Brahman cattle was rich ; the rate of nucleotide variation, hap...The complete mitochondrial DNA D-loop sequences from 10 stud Brahman cattle were sequenced and analyzed. The results showed that the genetic diversity of Brahman cattle was rich ; the rate of nucleotide variation, haplotype diversity and nucleotide diversity were 6.25%, 0.978± 0.054 and 0.014 30± 0.008 68, respectively. Nine haplotypes were defined and fell into two distinct lineages, suggesting that Brahman cattle have both Bos indicus （Zebu） and B. taurus genetic background. The taurine haplotypes were predominant at 90% and only Brah-6 belonged to the Asian zebu mthaplotype. This indicates that Brahman cattle was one of the zebu breeds and inherited the excellent characteristics of both the Asian zebu and European beef cattle, such as easy calf delivery, high quality beef, heat tolerance and resistance to various parasites. Breeders introduced Brahman cattle to improve the productivity and adaptability of native cattle. The Zebu has evidently frequently introgressed into the modem taurine breeds. As for modem zebu breeds, B. taurus also highly contributed to their formation, except for the Asian zebu. Furthermore our results also confirm the hypothesis that B. indicus has undergone a separate domestication event and originated from the Indian subcontinent.展开更多
Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male infertility.Thi...Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male infertility.This study,which contributes to the identification of genetic markers of sperm abnormalities,was conducted to study mtDNA mutations in the asthenozoospermia profile.Methods:This case-control study included 30 patients with asthenozoospermia and 28 with normospermia after spermogram and spermocytogram analyses.After the extraction of total DNA from the spermatozoa of 58 ejaculates from these individuals using the phenol-chloroform method,the amplification of genes of interest in mtDNA using specific primers was performed by conventional polymerase chain reaction,and sequencing was used to detect mutations.Results:Male patients with asthenozoospermia in the tertiary sector had significantly more mutant-than wild-type(P=0.0005)MT-CO II genes.Similarly,for the same gene,males with asthenozoospermia and primary infertility had significantly more mutants than the wild-type(P=0.001).Sequencing revealed 29 mutations that were observed only with asthenozoospermia,which could be the basis for low sperm mobility.Conclusion:This study identified several mutations in mtDNA genes that could be considered genetic markers of asthenozoospermia if confirmed in a deeper study.展开更多
文摘Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identifi ed. The genetic diversity was calculated to be 0.9691, and the genetic identity was calculated to be 0.0406. Conclusion There are some particul ar polymorphism sites in Chinese YUGU ethnic group. The results suggest that seq uence polymorphism from np16091-16418 in human mitochondrial DNA can be used as a biological marker for forensic identity.
基金Supported by the National Key Technologies R&D Program in the 9th five-year plan of China (No.96-919-01-04-3)
文摘Objective: To investigate the mitochondrial DNA sequence polymorphism in Chinese Dongxiang ethnic group and to provide basic data used in ethnic origin investigation and forensic purpose. Methods: Genomic DNA was extracted from the whole blood of 100 unrelated individuals of Chinese Dongxiang ethnic group by standard Chelex-100 method. The sequence polymorphism was determined by PCR amplification and direct sequencing. Results: Eighty-two polymorphic sites were identified in mtDNA D-loop region 16 091 - 16 418 np, and 88 haplotypes were found. The genetic diversity was calculated to he 0.9969, and the genetic identity was 0.013 2. Conclusion: There are some particular polymorphic sites in Chinese Dongxiang ethnic group, and these sites provide an important basis to investigate the origin of Dongxiang and the relationship between Dongxiang and other ethnic groups. The result also suggested that sequence polymorphism from 16 091 -16 418 np in human mitochondrial DNA control region can be an useful tool for forensic identity.
文摘The complete mitochondrial DNA D-loop sequences from 10 stud Brahman cattle were sequenced and analyzed. The results showed that the genetic diversity of Brahman cattle was rich ; the rate of nucleotide variation, haplotype diversity and nucleotide diversity were 6.25%, 0.978± 0.054 and 0.014 30± 0.008 68, respectively. Nine haplotypes were defined and fell into two distinct lineages, suggesting that Brahman cattle have both Bos indicus （Zebu） and B. taurus genetic background. The taurine haplotypes were predominant at 90% and only Brah-6 belonged to the Asian zebu mthaplotype. This indicates that Brahman cattle was one of the zebu breeds and inherited the excellent characteristics of both the Asian zebu and European beef cattle, such as easy calf delivery, high quality beef, heat tolerance and resistance to various parasites. Breeders introduced Brahman cattle to improve the productivity and adaptability of native cattle. The Zebu has evidently frequently introgressed into the modem taurine breeds. As for modem zebu breeds, B. taurus also highly contributed to their formation, except for the Asian zebu. Furthermore our results also confirm the hypothesis that B. indicus has undergone a separate domestication event and originated from the Indian subcontinent.
文摘Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male infertility.This study,which contributes to the identification of genetic markers of sperm abnormalities,was conducted to study mtDNA mutations in the asthenozoospermia profile.Methods:This case-control study included 30 patients with asthenozoospermia and 28 with normospermia after spermogram and spermocytogram analyses.After the extraction of total DNA from the spermatozoa of 58 ejaculates from these individuals using the phenol-chloroform method,the amplification of genes of interest in mtDNA using specific primers was performed by conventional polymerase chain reaction,and sequencing was used to detect mutations.Results:Male patients with asthenozoospermia in the tertiary sector had significantly more mutant-than wild-type(P=0.0005)MT-CO II genes.Similarly,for the same gene,males with asthenozoospermia and primary infertility had significantly more mutants than the wild-type(P=0.001).Sequencing revealed 29 mutations that were observed only with asthenozoospermia,which could be the basis for low sperm mobility.Conclusion:This study identified several mutations in mtDNA genes that could be considered genetic markers of asthenozoospermia if confirmed in a deeper study.
