[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny a...[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.展开更多
The purpose of this study was to investigate mitochondrial DNA (mtDNA) hypervariable segment-I (HVS-I) C-stretch variations and explore the significance of these variations in forensic and population genetics studies....The purpose of this study was to investigate mitochondrial DNA (mtDNA) hypervariable segment-I (HVS-I) C-stretch variations and explore the significance of these variations in forensic and population genetics studies. The C-stretch sequence variation was studied in 919 unrelated individuals from 8 Chinese ethnic groups using both direct and clone sequencing ap-proaches. Thirty eight C-stretch haplotypes were identified, and some novel and population specific haplotypes were also detected. The C-stretch genetic diversity (GD) values were relatively high, and probability (P) values were low. Additionally, C-stretch length heteroplasmy was observed in approximately 9% of individuals studied. There was a significant correlation (r=-0.961, P<0.01) between the expansion of the cytosine sequence length in the C-stretch of HVS-I and a reduction in the number of up-stream adenines. These results indicate that the C-stretch could be a useful genetic maker in forensic identification of Chinese populations. The results from the Fst and dA genetic distance matrix, neighbor-joining tree, and principal component map also suggest that C-stretch could be used as a reliable genetic marker in population genetics.展开更多
Present study describes the results of an efficient protocol for the isolation of good quality DNA from human saliva.The protocol includes collection of saliva in sterile specimen tubes,followed by the cell lysis.Afte...Present study describes the results of an efficient protocol for the isolation of good quality DNA from human saliva.The protocol includes collection of saliva in sterile specimen tubes,followed by the cell lysis.After formation of cell lysate,proteins wereextracted by phenol chloroform treatment for purification of DNA.The purified DNA was precipitated by adding equal volume of isopropanol to the treated supernatant.After isolation DNA pellet was washed with 70%ethanol,air-dried and was suspended in 30 pL of double distilled water.Best quality of DNA was extracted from the saliva samples and the PCR product was amplified for hyper-variable regions(HVI&HV2)of the mitochondrial DNA.The genes were cleaned with GeneAll gel elution kit(Gel SV)(Cat.No.102-10)and sequenced accordingly.The DNA isolation protocol presented here is recommended for the isolation,best quality and yield of DNA from the human saliva.展开更多
Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleo...Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleotide diversity were 0.608-1.000 and 0.002-0.028,respectively,indicating abundant genetic diversity in Chinese cattle.The constructed Neighbor-Joining(NJ) tree revealed that there were two maternal origins of Bos taurus and Bos indicus in Chinese cattle.Among them,the Bos taurus lineage had rich mtDNA genetic diversity,while Bos indicus lineage had poor mtDNA genetic diversity,respectively.It can be concluded that the cattle in Northern China originated from the Bos taurus lineage,while the cattle in Central China and Southern China originated from Bos taurus and Bos indicus lineages.In our study,two mtDNA haplotypes belonging to African cattle were detected,thus providing molecular evidence for an African lineage in Chinese cattle.展开更多
基金Supported by the Fond for Open Projects of Xinjiang Key Laboratory of Herbivore Nutrition for Meat&Milk Production~~
文摘[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.
基金supported by the Sciences and Technological Fundamental Resources Data of the Ministry of Education, China (No. 505015)the Key Project for Science and Technology of Shaanxi Province, China (No. 2004K09-G12)
文摘The purpose of this study was to investigate mitochondrial DNA (mtDNA) hypervariable segment-I (HVS-I) C-stretch variations and explore the significance of these variations in forensic and population genetics studies. The C-stretch sequence variation was studied in 919 unrelated individuals from 8 Chinese ethnic groups using both direct and clone sequencing ap-proaches. Thirty eight C-stretch haplotypes were identified, and some novel and population specific haplotypes were also detected. The C-stretch genetic diversity (GD) values were relatively high, and probability (P) values were low. Additionally, C-stretch length heteroplasmy was observed in approximately 9% of individuals studied. There was a significant correlation (r=-0.961, P<0.01) between the expansion of the cytosine sequence length in the C-stretch of HVS-I and a reduction in the number of up-stream adenines. These results indicate that the C-stretch could be a useful genetic maker in forensic identification of Chinese populations. The results from the Fst and dA genetic distance matrix, neighbor-joining tree, and principal component map also suggest that C-stretch could be used as a reliable genetic marker in population genetics.
文摘Present study describes the results of an efficient protocol for the isolation of good quality DNA from human saliva.The protocol includes collection of saliva in sterile specimen tubes,followed by the cell lysis.After formation of cell lysate,proteins wereextracted by phenol chloroform treatment for purification of DNA.The purified DNA was precipitated by adding equal volume of isopropanol to the treated supernatant.After isolation DNA pellet was washed with 70%ethanol,air-dried and was suspended in 30 pL of double distilled water.Best quality of DNA was extracted from the saliva samples and the PCR product was amplified for hyper-variable regions(HVI&HV2)of the mitochondrial DNA.The genes were cleaned with GeneAll gel elution kit(Gel SV)(Cat.No.102-10)and sequenced accordingly.The DNA isolation protocol presented here is recommended for the isolation,best quality and yield of DNA from the human saliva.
文摘Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleotide diversity were 0.608-1.000 and 0.002-0.028,respectively,indicating abundant genetic diversity in Chinese cattle.The constructed Neighbor-Joining(NJ) tree revealed that there were two maternal origins of Bos taurus and Bos indicus in Chinese cattle.Among them,the Bos taurus lineage had rich mtDNA genetic diversity,while Bos indicus lineage had poor mtDNA genetic diversity,respectively.It can be concluded that the cattle in Northern China originated from the Bos taurus lineage,while the cattle in Central China and Southern China originated from Bos taurus and Bos indicus lineages.In our study,two mtDNA haplotypes belonging to African cattle were detected,thus providing molecular evidence for an African lineage in Chinese cattle.
