Selecting the embryo with the highest implantation potential is a top priority in in-vitro fertilization(IVF)centers.Few studies have explored the relationship between day 5 blastocyst morphokinetics and implantation ...Selecting the embryo with the highest implantation potential is a top priority in in-vitro fertilization(IVF)centers.Few studies have explored the relationship between day 5 blastocyst morphokinetics and implantation outcomes[1].Despite numerous time-lapse studies,the findings often conflict due to differences in patient demographics,lab conditions,and protocols,such as oxygen concentration[2].Thus,there is ongoing debate regarding which parameters are most predictive of implantation.展开更多
Objective:To investigate morphokinetic characteristics of embryos displaying either reverse cleavage or direct cleavage during the first,second or third cleavage cycle.Methods:A total of 167 in vitro fertilization and...Objective:To investigate morphokinetic characteristics of embryos displaying either reverse cleavage or direct cleavage during the first,second or third cleavage cycle.Methods:A total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women[aged(35.0±4.6)years]were included for reverse cleavage analysis,and a total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women[aged(33.8±4.3)years]were included for direct cleavage analysis in this study.Using a sibling-embryo design,morphokinetic profiles(both before and after the onset of abnormal event)of embryos displaying reverse cleavage(n=241)or direct cleavage(n=244)were compared with their unaffected siblings(the controls)in the first,second and third cell cycles(n=32,n=142,n=562;n=195,n=412,n=205,respectively),at different developmental stages up to day 3.Results:Direct cleavage embryos demonstrated significantly delayed cleavage rates prior to the event regardless of developmental stage of the occurrence,while reverse cleavage embryos showed similar cleavage rates to their unaffected siblings.Post event,direct cleavage embryos sped up cleavage rates while reverse cleavage embryos slowed down.Conclusions:Altered morphokinetic profiles are displayed by direct cleavage embryos both before and after their occurrence and reverse cleavage embryos after the occurrence,which could potentially confound morphokinetic comparisons if not separated from their unaffected sibling embryos.Further study is warranted in order to fully understand the biological mechanisms of such events.展开更多
To investigate whether sperm with low concentration and motility can impact preimplantation embryos and to analyze how the effects present under a time-lapse incubation system,2905 oocytes were collected from 219 coup...To investigate whether sperm with low concentration and motility can impact preimplantation embryos and to analyze how the effects present under a time-lapse incubation system,2905 oocytes were collected from 219 couples between January 2014 and December 2015.Patients were divided into three groups according to sperm quality.Morphokinetic parameters and six cleavage patterns in the initial three cleavages were evaluated using the Primo Vision system.Embryo quality and clinic outcomes such as implantation rate,pregnancy rate and live birth rate were measured.The results showed that the concentration and motility of sperm correlated strongly with the rate of 2PN embryos,good-quality embryos on D3,blastocysts on D5/6 and good quality embryos on D5/6.The time-lapse system recordings showed that compromised sperm quality could result in a significant delay in cc l and a decrease in cc2,and impact embryo developmental potential mainly through large fragments or/and blastomere fragmentation in the initial three cleavages.In conclusion,sperm with low concentration and motility can have paternal effects on preimplantation embryos.These paternal effects present both as changes in morphokinetic parameters and cleavage patterns,which occur as early as fertilization and may cause severe damage to the preimplantation embryos.展开更多
文摘Selecting the embryo with the highest implantation potential is a top priority in in-vitro fertilization(IVF)centers.Few studies have explored the relationship between day 5 blastocyst morphokinetics and implantation outcomes[1].Despite numerous time-lapse studies,the findings often conflict due to differences in patient demographics,lab conditions,and protocols,such as oxygen concentration[2].Thus,there is ongoing debate regarding which parameters are most predictive of implantation.
文摘Objective:To investigate morphokinetic characteristics of embryos displaying either reverse cleavage or direct cleavage during the first,second or third cleavage cycle.Methods:A total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women[aged(35.0±4.6)years]were included for reverse cleavage analysis,and a total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women[aged(33.8±4.3)years]were included for direct cleavage analysis in this study.Using a sibling-embryo design,morphokinetic profiles(both before and after the onset of abnormal event)of embryos displaying reverse cleavage(n=241)or direct cleavage(n=244)were compared with their unaffected siblings(the controls)in the first,second and third cell cycles(n=32,n=142,n=562;n=195,n=412,n=205,respectively),at different developmental stages up to day 3.Results:Direct cleavage embryos demonstrated significantly delayed cleavage rates prior to the event regardless of developmental stage of the occurrence,while reverse cleavage embryos showed similar cleavage rates to their unaffected siblings.Post event,direct cleavage embryos sped up cleavage rates while reverse cleavage embryos slowed down.Conclusions:Altered morphokinetic profiles are displayed by direct cleavage embryos both before and after their occurrence and reverse cleavage embryos after the occurrence,which could potentially confound morphokinetic comparisons if not separated from their unaffected sibling embryos.Further study is warranted in order to fully understand the biological mechanisms of such events.
基金This work was supported by grants from the National Key Technology R&D Program of China(No.2019YFC1005200,No.2019YFC1005202,and No.2018YFC1002103)Hubei Province Health and Family Planning Scientific Research Project of China(No.WJ2019M127).
文摘To investigate whether sperm with low concentration and motility can impact preimplantation embryos and to analyze how the effects present under a time-lapse incubation system,2905 oocytes were collected from 219 couples between January 2014 and December 2015.Patients were divided into three groups according to sperm quality.Morphokinetic parameters and six cleavage patterns in the initial three cleavages were evaluated using the Primo Vision system.Embryo quality and clinic outcomes such as implantation rate,pregnancy rate and live birth rate were measured.The results showed that the concentration and motility of sperm correlated strongly with the rate of 2PN embryos,good-quality embryos on D3,blastocysts on D5/6 and good quality embryos on D5/6.The time-lapse system recordings showed that compromised sperm quality could result in a significant delay in cc l and a decrease in cc2,and impact embryo developmental potential mainly through large fragments or/and blastomere fragmentation in the initial three cleavages.In conclusion,sperm with low concentration and motility can have paternal effects on preimplantation embryos.These paternal effects present both as changes in morphokinetic parameters and cleavage patterns,which occur as early as fertilization and may cause severe damage to the preimplantation embryos.
