Monodispersed potymeric microparticles were prepared by seed-poly- merization.High performance packings were obtained for anion chromatography by coating the surface-sutfonated partictes with quarternized latexes.
Achieving multicolor,precisely tunable cluster-induced emission in nonconjugated polymers remains a considerable challenge.Herein,we present a generalizable and scalable methodology for fabricating monodisperse,color-...Achieving multicolor,precisely tunable cluster-induced emission in nonconjugated polymers remains a considerable challenge.Herein,we present a generalizable and scalable methodology for fabricating monodisperse,color-tunable clusteroluminescence(CL)microspheres,enabling multimodal color tuning across the spectrum from blue to orange-red through precise control of monomer type and ratio,sulfonation time,and pH conditions.Density functional theory(DFT)simulations demonstrate that conformational rigidity,resulting from the synergistic combination of prevalent hydrogen-bonding interactions,short interatomic contacts,and oxygen cluster formation,significantly enhances emission efficiency,leading to dual broadband visible emissions across the 400–700 nm wavelength range.The optimized sulfonated poly(divinylbenzene-styrene-methyl methacrylate)microspheres with 30%methyl methacrylate(MMA)content(SPSMMAs-30)exhibit excellent monodispersity and strong fluorescence across 13 standard channels of flow cytometry,with fluorescence coefficient of variation(CV)values consistently below 3%,fulfilling requirements for routine flow cytometer calibration.Compared with commercial calibration microspheres,SPSMMAs-30 show significantly higher photobleaching resistance and long-term environmental stability.Significantly,this protocol enables the first kilogram-scale synthesis of CLmicrospheres with highly reproducible optical properties.Furthermore,SPSMMAs-30 demonstrate sensitive tetracycline detection and promising performance in multicolor anticounterfeiting applications,substantially broadening the scope of nonconjugated CL materials for biomedicine,diagnostics,and materials science.展开更多
文摘Monodispersed potymeric microparticles were prepared by seed-poly- merization.High performance packings were obtained for anion chromatography by coating the surface-sutfonated partictes with quarternized latexes.
基金supported by the Ministry of Science and Technology of the People’s Republic of China(no.2022YFC2406600)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(no.Y2023088)the Innovative Key Project of Suzhou Institute of Biomedical Engineering and Technology,the Chinese Academy of Sciences(no.CX202501001).
文摘Achieving multicolor,precisely tunable cluster-induced emission in nonconjugated polymers remains a considerable challenge.Herein,we present a generalizable and scalable methodology for fabricating monodisperse,color-tunable clusteroluminescence(CL)microspheres,enabling multimodal color tuning across the spectrum from blue to orange-red through precise control of monomer type and ratio,sulfonation time,and pH conditions.Density functional theory(DFT)simulations demonstrate that conformational rigidity,resulting from the synergistic combination of prevalent hydrogen-bonding interactions,short interatomic contacts,and oxygen cluster formation,significantly enhances emission efficiency,leading to dual broadband visible emissions across the 400–700 nm wavelength range.The optimized sulfonated poly(divinylbenzene-styrene-methyl methacrylate)microspheres with 30%methyl methacrylate(MMA)content(SPSMMAs-30)exhibit excellent monodispersity and strong fluorescence across 13 standard channels of flow cytometry,with fluorescence coefficient of variation(CV)values consistently below 3%,fulfilling requirements for routine flow cytometer calibration.Compared with commercial calibration microspheres,SPSMMAs-30 show significantly higher photobleaching resistance and long-term environmental stability.Significantly,this protocol enables the first kilogram-scale synthesis of CLmicrospheres with highly reproducible optical properties.Furthermore,SPSMMAs-30 demonstrate sensitive tetracycline detection and promising performance in multicolor anticounterfeiting applications,substantially broadening the scope of nonconjugated CL materials for biomedicine,diagnostics,and materials science.
