Objective:To explore the effect of alcoholic extract of Bacopa monniera(AEBM) on high cholesterol diet-induced rats.Methods:The shade-dried and coarsely powdered whole plant material(Bacopa monniera) was extracted wit...Objective:To explore the effect of alcoholic extract of Bacopa monniera(AEBM) on high cholesterol diet-induced rats.Methods:The shade-dried and coarsely powdered whole plant material(Bacopa monniera) was extracted with 90%ethanol,finally filtered and dried in vacuum pump.The experimental rats were divided into 4 groups:control(group-Ⅰ),Rats fed with hvpercholesterolemic diet(HCD) for 45 days[4%cholesterol(w/w) and 1%cholic acid].Rats fed with HCD for 45 days+AEBM(40mg/kg,body weight/day orally) for last 30 days(group-Ⅲ) and AEBM alone(group-IV).Blood and tissues(Aorta) were removed to ice cold containers for various biochemical and histological analysis.Results:AEBM treatment significantly decreased the levels of TC,TG,PL,LDL,VLDL,atherogenic index,LDL/HDL ratio,and TC/HDL ratio but significantly increased the level of HDL when compared to HCD induced rats.Activities on liver antioxidant status(SOD,CAT,CPx,CR,GST) were significantly raised with concomitant reduction in the level of LPO were obtained in AEBM treated rats when compared to HCD rats.Treatment with AEBM significantly lowered the activity of SCOT,LDH and CPK.Histopathology of aorta of cholesterol fed rat showed intimal thickening and foam cell deposition were noted.Conclusions: These results suggests that AEBM extended protection against various biochemical changes and aortic pathology in hypercholesterolemic rats.Thus the plant may therefore be useful for therapeutic treatment of clinical conditions associated hypercholesterolemia.展开更多
A new triterpenoid saponin, bacopaside IX, was isolated from the whole plant of Bacopa monniera （L.） Wettst. and its structure was elucidated as 3-O-{β-d-glucopyranosyl（1 → 4）[α-1-arabinofuranosyl-（1 → 2）]-...A new triterpenoid saponin, bacopaside IX, was isolated from the whole plant of Bacopa monniera （L.） Wettst. and its structure was elucidated as 3-O-{β-d-glucopyranosyl（1 → 4）[α-1-arabinofuranosyl-（1 → 2）]-β-d-glucop-yranosyl}-20-O-α-1-arabinopyranosyljujubogenin by spectroscopic methods and some chemical transformations.展开更多
Bacopa monniera Linn. or Brahmi, a plant in the family Scrophulariaceae, is used as a traditional medicine for memory improvement, dementia like anxiety, depression, epilepsy etc. for a long time in Bangladesh. This s...Bacopa monniera Linn. or Brahmi, a plant in the family Scrophulariaceae, is used as a traditional medicine for memory improvement, dementia like anxiety, depression, epilepsy etc. for a long time in Bangladesh. This study evaluated the anxiolytic effects of the methanolic extract of B. monniera (MEBM) in animal models of anxiety. For the further tests and phytochemical screening, the whole plant of B. monniera extracted following maceration method. The extract was evaluated for anxiolytic activity using light/dark box, elevated plus maze, marble burying and rota rod tests in mice at the doses of 50, 100, and 200 mg/kg body weight. Diazepam (1.0 mg/kg), an anxiolytic drug used as standard drug. MEBM showed a strong and dose-dependent anxiolytic effects in animal models of anxiety. MEBM significantly increased the time spent in the light compartment, the latency time, and the entries of open arms in light/dark and elevated plus maze tests. In addition, MEBM also significantly decreased the number of buried marbles in marble burying test and increased the time of performance in rota rod test (p < 0.05). These were the major findings of this study. Therefore, our results reported that MEBM possesses anxiolytic effects in animal models of anxiety that support the traditional use of this plant as a potent agent to combat anxiety.展开更多
基金The financial support extended by UGC in the form of JRF for meritorious students under UGC-SAP programme
文摘Objective:To explore the effect of alcoholic extract of Bacopa monniera(AEBM) on high cholesterol diet-induced rats.Methods:The shade-dried and coarsely powdered whole plant material(Bacopa monniera) was extracted with 90%ethanol,finally filtered and dried in vacuum pump.The experimental rats were divided into 4 groups:control(group-Ⅰ),Rats fed with hvpercholesterolemic diet(HCD) for 45 days[4%cholesterol(w/w) and 1%cholic acid].Rats fed with HCD for 45 days+AEBM(40mg/kg,body weight/day orally) for last 30 days(group-Ⅲ) and AEBM alone(group-IV).Blood and tissues(Aorta) were removed to ice cold containers for various biochemical and histological analysis.Results:AEBM treatment significantly decreased the levels of TC,TG,PL,LDL,VLDL,atherogenic index,LDL/HDL ratio,and TC/HDL ratio but significantly increased the level of HDL when compared to HCD induced rats.Activities on liver antioxidant status(SOD,CAT,CPx,CR,GST) were significantly raised with concomitant reduction in the level of LPO were obtained in AEBM treated rats when compared to HCD rats.Treatment with AEBM significantly lowered the activity of SCOT,LDH and CPK.Histopathology of aorta of cholesterol fed rat showed intimal thickening and foam cell deposition were noted.Conclusions: These results suggests that AEBM extended protection against various biochemical changes and aortic pathology in hypercholesterolemic rats.Thus the plant may therefore be useful for therapeutic treatment of clinical conditions associated hypercholesterolemia.
基金supported by the National Natural Science Foundation of China(No.20002024)partially by the Science and Technology Developing Foundation of Shanghai(No.03QMH1414,00DZ19842,04DZ19856,04DZ19857,05DZ19733,06DZ19717,and 06DZ19005)Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT).
文摘A new triterpenoid saponin, bacopaside IX, was isolated from the whole plant of Bacopa monniera （L.） Wettst. and its structure was elucidated as 3-O-{β-d-glucopyranosyl（1 → 4）[α-1-arabinofuranosyl-（1 → 2）]-β-d-glucop-yranosyl}-20-O-α-1-arabinopyranosyljujubogenin by spectroscopic methods and some chemical transformations.
文摘Bacopa monniera Linn. or Brahmi, a plant in the family Scrophulariaceae, is used as a traditional medicine for memory improvement, dementia like anxiety, depression, epilepsy etc. for a long time in Bangladesh. This study evaluated the anxiolytic effects of the methanolic extract of B. monniera (MEBM) in animal models of anxiety. For the further tests and phytochemical screening, the whole plant of B. monniera extracted following maceration method. The extract was evaluated for anxiolytic activity using light/dark box, elevated plus maze, marble burying and rota rod tests in mice at the doses of 50, 100, and 200 mg/kg body weight. Diazepam (1.0 mg/kg), an anxiolytic drug used as standard drug. MEBM showed a strong and dose-dependent anxiolytic effects in animal models of anxiety. MEBM significantly increased the time spent in the light compartment, the latency time, and the entries of open arms in light/dark and elevated plus maze tests. In addition, MEBM also significantly decreased the number of buried marbles in marble burying test and increased the time of performance in rota rod test (p < 0.05). These were the major findings of this study. Therefore, our results reported that MEBM possesses anxiolytic effects in animal models of anxiety that support the traditional use of this plant as a potent agent to combat anxiety.
