AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： I...AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.展开更多
目的:探讨不同干预措施对ICU获得性衰弱(ICU-AW)的影响,为临床实践提供依据。方法:通过系统评价与荟萃分析,计算机检索PubMed、Embase、ScienceDirect、Web of Science、中国知网等数据库,收集相关主题系统评价,设定纳入标准与排除标准...目的:探讨不同干预措施对ICU获得性衰弱(ICU-AW)的影响,为临床实践提供依据。方法:通过系统评价与荟萃分析,计算机检索PubMed、Embase、ScienceDirect、Web of Science、中国知网等数据库,收集相关主题系统评价,设定纳入标准与排除标准,经文献筛选、信息提取、质量评价、有效性评价及证据图谱制作等步骤进行研究。结果:共纳入35项研究,多数优效及方法学质量较高的干预措施集中在早期运动和神经电生理学检查,17篇早期活动的Meta分析中有11篇显示其能优效改善ICU-AW,8篇神经肌肉电刺激相关文章中有6篇显示其可优效改善。结论:早期运动和神经肌肉电刺激是改善ICU-AW有效的干预措施,但受研究局限性影响,其有效性仍需进一步验证,未来需关注研究热点变化,调整研究方向,深入研究不同干预措施联合应用潜力。展开更多
文摘AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.
文摘目的:探讨不同干预措施对ICU获得性衰弱(ICU-AW)的影响,为临床实践提供依据。方法:通过系统评价与荟萃分析,计算机检索PubMed、Embase、ScienceDirect、Web of Science、中国知网等数据库,收集相关主题系统评价,设定纳入标准与排除标准,经文献筛选、信息提取、质量评价、有效性评价及证据图谱制作等步骤进行研究。结果:共纳入35项研究,多数优效及方法学质量较高的干预措施集中在早期运动和神经电生理学检查,17篇早期活动的Meta分析中有11篇显示其能优效改善ICU-AW,8篇神经肌肉电刺激相关文章中有6篇显示其可优效改善。结论:早期运动和神经肌肉电刺激是改善ICU-AW有效的干预措施,但受研究局限性影响,其有效性仍需进一步验证,未来需关注研究热点变化,调整研究方向,深入研究不同干预措施联合应用潜力。
