AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： I...AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.展开更多
突发公共事件期间病人涌入导致重症医学科(intensive care unit, ICU)救治效率降低。为综合评估病人床位分配策略提升ICU救治效率的效果,采用智能体建模-离散事件仿真(agent-based modeling-discrete event simulation, ABM-DES)方法构...突发公共事件期间病人涌入导致重症医学科(intensive care unit, ICU)救治效率降低。为综合评估病人床位分配策略提升ICU救治效率的效果,采用智能体建模-离散事件仿真(agent-based modeling-discrete event simulation, ABM-DES)方法构建ICU救治系统仿真模型。以武汉大学人民医院综合性ICU为例,引入急诊科分区管理策略设计4种差异化床位分配方案,同时从病人、医护人员、物资和空间多维度出发,采用熵权逼近理想解排序法(technique for order preference by similarity to an ideal solution, TOPSIS)法构建ICU救治效率评价体系对多种病人到达模式下的救治效率进行量化评估,筛选最优床位分配方案。结果表明:病人到达模式显著影响最优床位分配方案;分区管理策略的增效作用仅体现在长期大量病人到达场景。研究结果可为ICU应对突发公共事件提供重要参考。展开更多
文摘AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.
文摘突发公共事件期间病人涌入导致重症医学科(intensive care unit, ICU)救治效率降低。为综合评估病人床位分配策略提升ICU救治效率的效果,采用智能体建模-离散事件仿真(agent-based modeling-discrete event simulation, ABM-DES)方法构建ICU救治系统仿真模型。以武汉大学人民医院综合性ICU为例,引入急诊科分区管理策略设计4种差异化床位分配方案,同时从病人、医护人员、物资和空间多维度出发,采用熵权逼近理想解排序法(technique for order preference by similarity to an ideal solution, TOPSIS)法构建ICU救治效率评价体系对多种病人到达模式下的救治效率进行量化评估,筛选最优床位分配方案。结果表明:病人到达模式显著影响最优床位分配方案;分区管理策略的增效作用仅体现在长期大量病人到达场景。研究结果可为ICU应对突发公共事件提供重要参考。
