The release of root exudates(REs) provides an important source of soil organic carbon. This work revealed the molecular composition of REs of different plant species including alfalfa( Medicago sativa L.), bean( Phase...The release of root exudates(REs) provides an important source of soil organic carbon. This work revealed the molecular composition of REs of different plant species including alfalfa( Medicago sativa L.), bean( Phaseolus vulgaris L.), barley( Hordeum vulgare L.), maize( Zea mays), wheat( Triticum aestivum L.), ryegrass( Lolium perenne L.) and pumpkin( Cucurbita maxima) using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry(ESI FT-ICR MS). The combination of positive ion mode( + ESI) and negative ion mode(-ESI) increased the number of the molecules detected by ESI FT-ICR MS, and a total of 8758 molecules were identified across all the samples. In detail, lipids and proteins and unsaturated hydrocarbons were more easily detected in + ESI mode, while aromatic compounds with high O/C were readily ionized in-ESI mode, and only 38% of the total assigned formulas were shared by -ESI and + ESI modes. Multivariate statistical analysis of the formulas indicated that the close related plants species secreted REs with similar molecular components. Moreover, the unsaturation degree and nitrogen content were the two key parameters able to distinguish the similarities and differences of molecular components of REs between plant species. The results provided a feasible analysis method for characterization of the molecular components of REs and for the first time characterized the molecular components of REs of a variety of plant species using ESI FT-ICR MS.展开更多
Neuroendocrine differentiation （NED）, which is not uncommon in prostate cancer, is increases in prostate cancer after androgen-deprivation therapy （ADT） and generally appears in castration- resistant prostate canc...Neuroendocrine differentiation （NED）, which is not uncommon in prostate cancer, is increases in prostate cancer after androgen-deprivation therapy （ADT） and generally appears in castration- resistant prostate cancer （CRPC）. Neuroendocrine ceils, which are found in normal prostate tissue, are a small subset of cells and have unique function in regulating the growth of prostate ceils. Prostate cancer with NED includes different types of tumor, including focal NED, pure neuroendocrine tumor or mixed neuroendocrine-adenocarcinoma. Although more and more studies are carried out on NED in prostate cancer, the molecular components that are involved in NED are still poorly elucidated. We review neuroendocrine cells in normal prostate tissue, NED in prostate cancer, terminology of NED and biomarkers used for detecting NED in routine pathological practice. Some recently reported molecular components which drive NED in prostate cancer are listed in the review.展开更多
Luminescent Single-Molecule Magnets(SMMs)have gained broad scientific attention due to their potential applications in the dual sensing of temperature and magnetic field or optical thermometry for the self-monitoring ...Luminescent Single-Molecule Magnets(SMMs)have gained broad scientific attention due to their potential applications in the dual sensing of temperature and magnetic field or optical thermometry for the self-monitoring of temperature in SMM-based devices.We present a route toward thermoresponsive emissive SMMs based on linking two molecular components separately providing luminescent thermometry and SMM features.展开更多
Liquid biopsy has emerged as a transformative,minimally invasive strategy for disease detection and longitudinal monitoring through the analysis of circulating molecular components,including cell-free DNA(cfDNA),cell-...Liquid biopsy has emerged as a transformative,minimally invasive strategy for disease detection and longitudinal monitoring through the analysis of circulating molecular components,including cell-free DNA(cfDNA),cell-free RNA,extracellular vesicles,and circulating proteins in bodily fluids.Its central objective is to enable a continuum of precision clinical interventions,from early detection and diagnosis to therapeutic guidance and disease surveillance,thereby improving patient outcomes(Figure 1).展开更多
基金supported by the National Key Research and Development Program of China (No. 2018YFC1800701)the National Natural Science Foundation of China (Nos. 21537005,21621064 and 21876187)。
文摘The release of root exudates(REs) provides an important source of soil organic carbon. This work revealed the molecular composition of REs of different plant species including alfalfa( Medicago sativa L.), bean( Phaseolus vulgaris L.), barley( Hordeum vulgare L.), maize( Zea mays), wheat( Triticum aestivum L.), ryegrass( Lolium perenne L.) and pumpkin( Cucurbita maxima) using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry(ESI FT-ICR MS). The combination of positive ion mode( + ESI) and negative ion mode(-ESI) increased the number of the molecules detected by ESI FT-ICR MS, and a total of 8758 molecules were identified across all the samples. In detail, lipids and proteins and unsaturated hydrocarbons were more easily detected in + ESI mode, while aromatic compounds with high O/C were readily ionized in-ESI mode, and only 38% of the total assigned formulas were shared by -ESI and + ESI modes. Multivariate statistical analysis of the formulas indicated that the close related plants species secreted REs with similar molecular components. Moreover, the unsaturation degree and nitrogen content were the two key parameters able to distinguish the similarities and differences of molecular components of REs between plant species. The results provided a feasible analysis method for characterization of the molecular components of REs and for the first time characterized the molecular components of REs of a variety of plant species using ESI FT-ICR MS.
基金supported by the National Natural Science Foundation of China (81502244)
文摘Neuroendocrine differentiation （NED）, which is not uncommon in prostate cancer, is increases in prostate cancer after androgen-deprivation therapy （ADT） and generally appears in castration- resistant prostate cancer （CRPC）. Neuroendocrine ceils, which are found in normal prostate tissue, are a small subset of cells and have unique function in regulating the growth of prostate ceils. Prostate cancer with NED includes different types of tumor, including focal NED, pure neuroendocrine tumor or mixed neuroendocrine-adenocarcinoma. Although more and more studies are carried out on NED in prostate cancer, the molecular components that are involved in NED are still poorly elucidated. We review neuroendocrine cells in normal prostate tissue, NED in prostate cancer, terminology of NED and biomarkers used for detecting NED in routine pathological practice. Some recently reported molecular components which drive NED in prostate cancer are listed in the review.
基金financed by the National Science Centre of Poland,the OPUS-21 project,no.2021/41/B/ST5/02544The research has been supported by an infrastructure grant from the Faculty of Chemistry under the Strategic Programme Excellence Initiative at Jagiellonian Univ.The study was carried out using the research infrastructure co-funded by the EU in the framework of the Smart Growth Operational Program,Measure 4.2+1 种基金Grant No.POIR.04.02.00-00-D001/20,“ATOMIN 2.0-ATOMic scale science for the INnovative economy”We also gratefully acknowledge Polish high-performance computing infrastructure PLGrid(HPC Center:ACK Cyfronet AGH)for providing computer facilities and support within computational grant no.PLG/2019/013095.
文摘Luminescent Single-Molecule Magnets(SMMs)have gained broad scientific attention due to their potential applications in the dual sensing of temperature and magnetic field or optical thermometry for the self-monitoring of temperature in SMM-based devices.We present a route toward thermoresponsive emissive SMMs based on linking two molecular components separately providing luminescent thermometry and SMM features.
基金supported by the National Natural Science Foundation of China(Grant Nos.82330073 and 82402731).
文摘Liquid biopsy has emerged as a transformative,minimally invasive strategy for disease detection and longitudinal monitoring through the analysis of circulating molecular components,including cell-free DNA(cfDNA),cell-free RNA,extracellular vesicles,and circulating proteins in bodily fluids.Its central objective is to enable a continuum of precision clinical interventions,from early detection and diagnosis to therapeutic guidance and disease surveillance,thereby improving patient outcomes(Figure 1).
