With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a c...With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.展开更多
To evaluate the therapeutic effect of a modified Devine procedure with a subcutaneous sliding fixation method for the treatment of congenital concealed penis,we retrospectively selected 45 patients with congenital con...To evaluate the therapeutic effect of a modified Devine procedure with a subcutaneous sliding fixation method for the treatment of congenital concealed penis,we retrospectively selected 45 patients with congenital concealed penises who were admitted to General Hospital of Ningxia Medical University(Yinchuan,China)between September 2020 and November 2023.In all cases,the penis was observed to be short,and retracting the skin at the base revealed a normal penile body,which immediately returned to its original position upon release.All patients underwent the modified Devine procedure with subcutaneous sliding fixation and completed a 12-week postoperative follow-up.A statistically significant increase in penile length was observed postoperatively,with the median length increasing from 4.0(interquartile range[IQR]:3.5–4.8;95%confidence interval[CI]:3.9–4.4)cm to 8.0(IQR:7.8–8.0;95%CI:7.7–7.9)cm,with P<0.001.The parents were satisfied with the outcomes,including increased penile length,improved hygiene,and enhanced esthetics.Except for mild foreskin edema in all cases,no complications(such as infections,skin necrosis,or penile retraction)were observed.The edema was resolved within 4 weeks after the operation.This study demonstrates that the modified Devine procedure utilizing the subcutaneous sliding fixation method yields excellent outcomes with minimal postoperative complications,reduced penile retraction,and high satisfaction rates among patients and their families.展开更多
This paper aims to evaluate the stochastic response of steel columns subjected to blast loads using the modified single degree of freedom(MSDOF)method,which assessed towards the conventional single degree of freedom(S...This paper aims to evaluate the stochastic response of steel columns subjected to blast loads using the modified single degree of freedom(MSDOF)method,which assessed towards the conventional single degree of freedom(SDOF)and the experimentally validated Finite Element(FE)methods(LSDYNA).For this purpose,special atten-tion is given to calculating the response of H-shaped steel columns under blast.The damage amount is determined based on the support rotation criterion,which is expressed as a function of their maximum lateral mid-span dis-placement.To account for uncertainties in input parameters and obtain the failure probability,the Monte Carlo simulation(MCS)method is employed,complemented by the Latin Hypercube Sampling(LHS)method to reduce the number of simulations.A parametric analysis is hence performed to examine the effect of several input pa-rameters(including both deterministic and probabilistic parameters)on the probability of column damage as a function of support rotation.First,the MSDOF method confirms its higher accuracy in estimating the probability of column damage due to blast,compared to the conventional SDOF.The collected results also show that un-certainties of several input parameters have significant effects on the column behavior.In particular,geometric parameters(including cross-sectional characteristics,boundary conditions and column length)have major effect on the corresponding column response,in the same way of input blast load parameters and material properties.展开更多
Cell cultured meat has been extensively studied as an environmentally friendly,energy-saving and more effective technology.However,there are many technical bottlenecks,especially the regulatory mechanism and manufactu...Cell cultured meat has been extensively studied as an environmentally friendly,energy-saving and more effective technology.However,there are many technical bottlenecks,especially the regulatory mechanism and manufacturing method of in vitro myogenesis.Based on an edible modified silk protein scaffold,with 3D culturing,in situ differentiated and transcriptome analysis,this study describes novel scaffolds and fabrication methods for cell cultured meat.The results showed that the effective space and utilization efficiency for cell culture of the scaffold is 26–1000 that of the traditional culture dish;it could form a tissue-like structure.Transcriptomics revealed the regulatory pathways and key factors of different cycles.It clarifies that the multi-cycle process of myoblast myogenesis in vitro is different from the single feedback regulation in vivo.More importantly,a novel scaffold-based cell cultured meat manufacturing method was developed,further develop a new tissue culture solution that is different from existing cell culture meat production.For manufacturing processes,it provides a new cell culture meat technology system,provides a theoretical basis for the regulation of cell proliferation and muscle growth,and lays the technical foundation for in situ tissue culture of cell cultured meat in vitro.展开更多
In this article, we consider a backward problem in time of the diffusion equation with local and nonlocal operators. This inverse problem is ill-posed because the solution does not depend continuously on the measured ...In this article, we consider a backward problem in time of the diffusion equation with local and nonlocal operators. This inverse problem is ill-posed because the solution does not depend continuously on the measured data. Inspired by the classical Landweber iterative method and Fourier truncation technique, we develops a modified Landweber iterative regularization method to restore the continuous dependence of solution on the measurement data. Under the a-priori and a-posteriori choice rules for the regularized parameter, the convergence estimates for the regularization method are derived. Some results of numerical simulation are provided to verify the stability and feasibility of our method in dealing with the considered problem.展开更多
Using a modified subgradient extragradient algorithm, this paper proposed a novel approach to solving a supply chain network equilibrium model. The method extends the scope of optimisation and improves the accuracy at...Using a modified subgradient extragradient algorithm, this paper proposed a novel approach to solving a supply chain network equilibrium model. The method extends the scope of optimisation and improves the accuracy at each iteration by incorporating adaptive parameter selection and a more general subgradient projection operator. The advantages of the proposed method are highlighted by the proof of strong convergence presented in the paper. Several concrete examples are given to demonstrate the effectiveness of the algorithm, with comparisons illustrating its superior CPU running time compared to alternative techniques. The practical applicability of the algorithm is also demonstrated by applying it to a realistic supply chain network model.展开更多
[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhiv...[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [ Result] The clear and orderly bands were observed in gel detection, and the values of OD200/OD200 for DNAs extracted via modified CTAB method were between 1.77 - 1.83. The DNAs performed well in PCR amplification. [ Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.展开更多
ObjectiveThe aim was to seek for a rapid DNA minipreparation method from wheat leaf. MethodThe total DNA of wheat leaf was extracted using CTAB, SDS and boiling water, separately, with some modifications. Integrity an...ObjectiveThe aim was to seek for a rapid DNA minipreparation method from wheat leaf. MethodThe total DNA of wheat leaf was extracted using CTAB, SDS and boiling water, separately, with some modifications. Integrity and purity of nucleic acids were detected through agarose gel electrophoresis, ultraviolet absorption and PCR. ResultThe DNA extracted by the modified CTAB method had high quality and purity, and was not degraded. Two hundreds of DNA samples could be extracted each workday by per capita using this method; and the PCR detection of wheat transgenic plants showed that amplified bands of target gene were clear, without false-positive, and the test results were satisfactory. The DNA purity and concentration extracted by modified SDS method were not as good as that extracted by modified CTAB method, but it also met the DNA requirements of major molecular research. The DNA quantity extracted by modified boiling method was small and there were a lot of impurities in it, PCR detection of this DNA showed no amplified band. ConclusionModified CTAB method is a simple and rapid method for DNA minipreparation from wheat leaf, and was suitable for PCR amplification and other molecular biology researches.展开更多
A modified domain reduction method(MDRM) that introduces damping terms to the original DRM is presented in this paper. To verify the proposed MDRM and compare the computational accuracy of these two methods, a numeric...A modified domain reduction method(MDRM) that introduces damping terms to the original DRM is presented in this paper. To verify the proposed MDRM and compare the computational accuracy of these two methods, a numerical test is designed. The numerical results of the MDRM and DRM are compared using an extended meshed model. The results show that the MDRM significantly improved the computational accuracy of the DRM. Then, the MDRM is compared with two existing conventional methods, namely Liao's transmitting boundary and viscous-spring boundary with Liu's method. The MDRM shows its great advancement in computational accuracy, stability and range of applications. This paper also discusses the influence of boundary location on computational accuracy. It can be concluded that smaller models tend to have larger errors. By introducing two dimensionless parameters, φ_1 and φ_2, the rational distance between the observation point and the MDRM boundary is suggested. When φ_1 >2 or φ_2>13, the relative PGA error can be limited to 5%. In practice, the appropriate model size can be chosen based on these two parameters to achieve desired computational accuracy.展开更多
Wind loading is one of the most important loads for controlling the design of large-span roof structures. Equivalent static wind loads, which can generally aim at determining a specific response, are widely used by st...Wind loading is one of the most important loads for controlling the design of large-span roof structures. Equivalent static wind loads, which can generally aim at determining a specific response, are widely used by structural designers. A method for equivalent static wind loads applicable to multi-responses is proposed in this paper. A modified load- response-correlation （LRC） method corresponding to a particular peak response is presented, and the similarity algorithm implemented for the group response is described. The main idea of the algorithm is that two responses can be put into one group if the value of one response is close to that of the other response, when the structure is subjected to equivalent static wind loads aiming at the other response. Based on the modified LRC, the grouping response method is put forward to construct equivalent static wind loading. This technique can simultaneously reproduce peak responses for some grouped responses. To verify its computational accuracy, the method is applied to an actual large-span roof structure. Calculation results show that when the similarity of responses in the same group is high, equivalent static wind loads with high accuracy and reasonable magnitude of equivalent static wind distribution can be achieved.展开更多
The density of Ni-AI alloys in both liquid state and solid-liquid coexistence state was measured with a modified pycnometric method. It was found that the density of Ni-AI alloys decreases with increasing temperature ...The density of Ni-AI alloys in both liquid state and solid-liquid coexistence state was measured with a modified pycnometric method. It was found that the density of Ni-AI alloys decreases with increasing temperature and Al concentration in the alloys. The molar volume of liquid Ni-AI binary alloys increases with the increase of temperature and Al concentration. The partial molar volume of Al in Ni-AI binary alloy was calculated approximately. The molar volume of liquid Ni-AI alloy determined in the present work shows a negative deviation from the ideal linear molar volume.展开更多
In actual power systems,most of the high-voltage buses of the transformers are zero injection buses without load or generation.Power injections into these buses are strictly 0,so based on Kirchhoff's current law(K...In actual power systems,most of the high-voltage buses of the transformers are zero injection buses without load or generation.Power injections into these buses are strictly 0,so based on Kirchhoff's current law(KCL),equality constraints should be used to handle these buses in a state estimation model.It is a challenge to ensure that these zero injection constraints can be strictly satisfied without losing computational efficiency.展开更多
The modified suction caisson(MSC) adds a short-skirted structure around the regular suction caissons to increase the lateral bearing capacity and limit the deflection. The MSC is suitable for acting as the offshore wi...The modified suction caisson(MSC) adds a short-skirted structure around the regular suction caissons to increase the lateral bearing capacity and limit the deflection. The MSC is suitable for acting as the offshore wind turbine foundation subjected to larger lateral loads compared with the imposed vertical loads. Determination of the lateral bearing capacity is a key issue for the MSC design. The formula estimating the lateral bearing capacity of the MSC was proposed in terms of the limit equilibrium method and was verified by the test results. Parametric studies on the lateral bearing capacity were also carried out. It was found that the lateral bearing capacity of the MSC increases with the increasing length and radius of the external skirt, and the lateral bearing capacity increases linearly with the increasing coefficient of subgrade reaction. The maximum lateral bearing capacity of the MSC is attained when the ratio of the radii of the internal compartment to the external skirt equals 0.82 and the ratio of the lengths of the external skirt to the internal compartment equals 0.48, provided that the steel usage of the MSC is kept constant.展开更多
The density of liquid binary Ni-Mo alloys with molybdenum concentration from 0 to 20% (mass fraction) wasmeasured by a modified sessile drop method. It has been found that the density of the liquid Ni-Mo alloys decrea...The density of liquid binary Ni-Mo alloys with molybdenum concentration from 0 to 20% (mass fraction) wasmeasured by a modified sessile drop method. It has been found that the density of the liquid Ni-Mo alloys decreaseswith increasing temperature, but increases with the increase of molybdenum concentration in the alloys. The molarvolume of liquid Ni-Mo binary alloys increases with the increase of temperature and molybdenum concentration. Thepartial molar volume of molybdenum in Ni-Mo binary alloy has been approximately calculated as [13.18-2.65×10^(-3)T+(-47.94+3.10×10^(-2)T)×10^(-2)X_(Mo)]×10^(-6)m^3·mol^(-1). The molar volume of Ni-Mo alloy determined inthe present work shows a negative deviation from the ideal linear mixing molar volume.展开更多
The stiffness spreading method (SSM) was initially proposed for layout optimization of truss structures,in which an artificial elastic material of low modulus is uniformly distributed in the design domain to create co...The stiffness spreading method (SSM) was initially proposed for layout optimization of truss structures,in which an artificial elastic material of low modulus is uniformly distributed in the design domain to create connections between discrete members.In this paper,a modified stiffness spreading method is proposed by replacing the artificial elastic material with auxiliary bars to connect real members of the truss structure.Since the background continuum mesh for the elastic material is no longer required,the computational cost is significantly reduced.Like SSM,the new method is advantageous in that an initial design may consist of disconnected bars allocated in the design domain,and mathematical programming methods can be applied for the efficient solution of the formulated optimization problem.A number of solution strategies are also developed to achieve more practical designs with lower computational cost.Numerical examples of both 2-D and 3-D truss structures are presented to demonstrate the feasibility,robustness and effectiveness of the proposed method.展开更多
By introducing a more general auxiliary ordinary differential equation （ODE）, a modified variable separated ODE method is developed for solving the mKdV-sinh-Gordon equation. As a result, many explicit and exact sol...By introducing a more general auxiliary ordinary differential equation （ODE）, a modified variable separated ODE method is developed for solving the mKdV-sinh-Gordon equation. As a result, many explicit and exact solutions including some new formal solutions are successfully picked up for the mKdV-sinh-Gordon equation by this approach.展开更多
The integrated structure parts are widely used in aircraft. The distortion caused by residual stresses in thick pre-stretched aluminum plates during machining integrated parts is a common and serious problem. To predi...The integrated structure parts are widely used in aircraft. The distortion caused by residual stresses in thick pre-stretched aluminum plates during machining integrated parts is a common and serious problem. To predict and control the machining distortion, the residual stress distribution in the thick plate must be measured firstly. The modified removal method for measuring residual stress in thick pre-stretched aluminum plates is proposed and the stress-strain relation matrix is deduced by elasticity theory. The residual stress distribution in specimen of 7050T7451 plate is measured by using the method, and measurement results are analyzed and compared with data obtained by other methods. The method is effective to measure the residual stress.展开更多
On the basis of hydrographic data obtained in August 2000 cruise, the circulation in the South China Sea (SCS) is computed by the modified inverse method in combination with SSH data from TOPEX/ERS-2 analysis. For stu...On the basis of hydrographic data obtained in August 2000 cruise, the circulation in the South China Sea (SCS) is computed by the modified inverse method in combination with SSH data from TOPEX/ERS-2 analysis. For study of the dynamical mechanism, which causes the pattern of summer circulation in the SCS, the diagnostic model (Yuan et al. 1982. Acta Oceanologica Sinica,4(1):1-11; Yuan and Su. 1992. Numerical Computation of Physical Oceanography.474-542) is used to simulate numerically the summer circulation in the SCS. The following results have been obtained. (1) The central and southwestern SCSs are dominated mainly by anticy-clonic circulation systems. They are mainly as follows. 1) There is strong anticyclonic eddy southeast of Vietnam (W1). Its horizontal scale is about 300 km, and it extends vertically from the surface to the about 1 000 m level. 2) There are a warm eddy W2 southeast of Zhongsha Islands and the anticyclonic circulation system W3 west off the Luzon Island. 3) There is a stronger cyclonic eddy C1 between the anticyclonic eddies W1 and W2.4) A strong northward coastal jet is present near the coast of Vietnam, and separates from the coast of Vietnam at about 12° N to the northeast.(2)The northern SCS is dominated mainly by a cyclonic circulation system. There is a cyclonic circulation system near and north of Section N2. (3) The southeastern SCS is dominated mainly by the cyclonic circulation system. (4) Comparing the results of circulation in the SCS during the summer of 2000 with those during the summer of 1998, it is found that they agree qualitatively, but there is the some difference between them in quantity.This shows that the circulation in the SCS has obviously seasonal feature. (5) The dynamical mechanism which products the basic pattern of summer circulation is because the following two reasons: 1) the joint effect of the baroclinity and relief (JEBAR) is essential dynamical cause; and 2) it is next important dynamical cause that the interaction between the wind stress and bottom topography under the southerly monsoon. (6) Comparing the hydrographic structure and distribution of stream functions with the SSH data from TOPEX/ERS-2 analysis in the SCS during August of 2000, they agree qualitatively.展开更多
A modified slow-fast analysis method is presented for the periodically excited non-autonomous dynamical system with an order gap between the exciting frequency and the natural frequency.By regarding the exciting term ...A modified slow-fast analysis method is presented for the periodically excited non-autonomous dynamical system with an order gap between the exciting frequency and the natural frequency.By regarding the exciting term as a slow-varying parameter,a generalized autonomous fast subsystem can be defined,the equilibrium branches as well as the bifurcations of which can be employed to account for the mechanism of the bursting oscillations by combining the transformed phase portrait introduced.As an example,a typical periodically excited Hartley model is used to demonstrate the validness of the method,in which the exciting frequency is far less than the natural frequency.The equilibrium branches and their bifurcations of the fast subsystem with the variation of the slow-varying parameter are presented.Bursting oscillations for two typical cases are considered,which reveals that,fold bifurcation may cause the the trajectory to jump between different equilibrium branches,while Hopf bifurcation may cause the trajectory to oscillate around the stable limit cycle.展开更多
基金the Experimental Technology Research Project of Zhejiang University(SYB202138)National Natural Science Foundation of China(32000195)。
文摘With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.
基金supported by the National Natural Science Foundation of China(No.81860268,and No.82201000)Ningxia Natural Science Foundation(No.2021AAC02025)+2 种基金Ningxia science and technology innovation leading talent training project(No.2020GKLRLX06 and No.2020GKLRLX11)Ningxia Medical University research project(No.XTJKF240315)Ningxia Key Research and Development Project(No.2023BEG03021 and No.2021BEB04034).
文摘To evaluate the therapeutic effect of a modified Devine procedure with a subcutaneous sliding fixation method for the treatment of congenital concealed penis,we retrospectively selected 45 patients with congenital concealed penises who were admitted to General Hospital of Ningxia Medical University(Yinchuan,China)between September 2020 and November 2023.In all cases,the penis was observed to be short,and retracting the skin at the base revealed a normal penile body,which immediately returned to its original position upon release.All patients underwent the modified Devine procedure with subcutaneous sliding fixation and completed a 12-week postoperative follow-up.A statistically significant increase in penile length was observed postoperatively,with the median length increasing from 4.0(interquartile range[IQR]:3.5–4.8;95%confidence interval[CI]:3.9–4.4)cm to 8.0(IQR:7.8–8.0;95%CI:7.7–7.9)cm,with P<0.001.The parents were satisfied with the outcomes,including increased penile length,improved hygiene,and enhanced esthetics.Except for mild foreskin edema in all cases,no complications(such as infections,skin necrosis,or penile retraction)were observed.The edema was resolved within 4 weeks after the operation.This study demonstrates that the modified Devine procedure utilizing the subcutaneous sliding fixation method yields excellent outcomes with minimal postoperative complications,reduced penile retraction,and high satisfaction rates among patients and their families.
文摘This paper aims to evaluate the stochastic response of steel columns subjected to blast loads using the modified single degree of freedom(MSDOF)method,which assessed towards the conventional single degree of freedom(SDOF)and the experimentally validated Finite Element(FE)methods(LSDYNA).For this purpose,special atten-tion is given to calculating the response of H-shaped steel columns under blast.The damage amount is determined based on the support rotation criterion,which is expressed as a function of their maximum lateral mid-span dis-placement.To account for uncertainties in input parameters and obtain the failure probability,the Monte Carlo simulation(MCS)method is employed,complemented by the Latin Hypercube Sampling(LHS)method to reduce the number of simulations.A parametric analysis is hence performed to examine the effect of several input pa-rameters(including both deterministic and probabilistic parameters)on the probability of column damage as a function of support rotation.First,the MSDOF method confirms its higher accuracy in estimating the probability of column damage due to blast,compared to the conventional SDOF.The collected results also show that un-certainties of several input parameters have significant effects on the column behavior.In particular,geometric parameters(including cross-sectional characteristics,boundary conditions and column length)have major effect on the corresponding column response,in the same way of input blast load parameters and material properties.
基金funded under the National key research and development plan(2021YFC2101404)Chinese Academy of Engineering Strategic Research and Consulting Project(2023-XZ-79,2022-30-19).
文摘Cell cultured meat has been extensively studied as an environmentally friendly,energy-saving and more effective technology.However,there are many technical bottlenecks,especially the regulatory mechanism and manufacturing method of in vitro myogenesis.Based on an edible modified silk protein scaffold,with 3D culturing,in situ differentiated and transcriptome analysis,this study describes novel scaffolds and fabrication methods for cell cultured meat.The results showed that the effective space and utilization efficiency for cell culture of the scaffold is 26–1000 that of the traditional culture dish;it could form a tissue-like structure.Transcriptomics revealed the regulatory pathways and key factors of different cycles.It clarifies that the multi-cycle process of myoblast myogenesis in vitro is different from the single feedback regulation in vivo.More importantly,a novel scaffold-based cell cultured meat manufacturing method was developed,further develop a new tissue culture solution that is different from existing cell culture meat production.For manufacturing processes,it provides a new cell culture meat technology system,provides a theoretical basis for the regulation of cell proliferation and muscle growth,and lays the technical foundation for in situ tissue culture of cell cultured meat in vitro.
基金supported by the NSF of Ningxia(2022AAC03234)the NSF of China(11761004),the Construction Project of First-Class Disciplines in Ningxia Higher Education(NXYLXK2017B09)the Postgraduate Innovation Project of North Minzu University(YCX23074).
文摘In this article, we consider a backward problem in time of the diffusion equation with local and nonlocal operators. This inverse problem is ill-posed because the solution does not depend continuously on the measured data. Inspired by the classical Landweber iterative method and Fourier truncation technique, we develops a modified Landweber iterative regularization method to restore the continuous dependence of solution on the measurement data. Under the a-priori and a-posteriori choice rules for the regularized parameter, the convergence estimates for the regularization method are derived. Some results of numerical simulation are provided to verify the stability and feasibility of our method in dealing with the considered problem.
文摘Using a modified subgradient extragradient algorithm, this paper proposed a novel approach to solving a supply chain network equilibrium model. The method extends the scope of optimisation and improves the accuracy at each iteration by incorporating adaptive parameter selection and a more general subgradient projection operator. The advantages of the proposed method are highlighted by the proof of strong convergence presented in the paper. Several concrete examples are given to demonstrate the effectiveness of the algorithm, with comparisons illustrating its superior CPU running time compared to alternative techniques. The practical applicability of the algorithm is also demonstrated by applying it to a realistic supply chain network model.
文摘[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [ Result] The clear and orderly bands were observed in gel detection, and the values of OD200/OD200 for DNAs extracted via modified CTAB method were between 1.77 - 1.83. The DNAs performed well in PCR amplification. [ Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.
基金Supported by Major National Transgenic Breeding Project(2011ZX08002-001)the Agricultural Science and Technology Support Program of Jiangsu Province(BE2011306)Agricultural Science and Technology Independent Innovation Fund ofJiangsu Province[CX(12)2026]~~
文摘ObjectiveThe aim was to seek for a rapid DNA minipreparation method from wheat leaf. MethodThe total DNA of wheat leaf was extracted using CTAB, SDS and boiling water, separately, with some modifications. Integrity and purity of nucleic acids were detected through agarose gel electrophoresis, ultraviolet absorption and PCR. ResultThe DNA extracted by the modified CTAB method had high quality and purity, and was not degraded. Two hundreds of DNA samples could be extracted each workday by per capita using this method; and the PCR detection of wheat transgenic plants showed that amplified bands of target gene were clear, without false-positive, and the test results were satisfactory. The DNA purity and concentration extracted by modified SDS method were not as good as that extracted by modified CTAB method, but it also met the DNA requirements of major molecular research. The DNA quantity extracted by modified boiling method was small and there were a lot of impurities in it, PCR detection of this DNA showed no amplified band. ConclusionModified CTAB method is a simple and rapid method for DNA minipreparation from wheat leaf, and was suitable for PCR amplification and other molecular biology researches.
基金National Natural Science Foundation of China under Grant Nos.91315301,51478279the State Key Laboratory Basic Theory Foundation of the Ministry of Science and Technology of China under the Grant SLDRCE08-A-07
文摘A modified domain reduction method(MDRM) that introduces damping terms to the original DRM is presented in this paper. To verify the proposed MDRM and compare the computational accuracy of these two methods, a numerical test is designed. The numerical results of the MDRM and DRM are compared using an extended meshed model. The results show that the MDRM significantly improved the computational accuracy of the DRM. Then, the MDRM is compared with two existing conventional methods, namely Liao's transmitting boundary and viscous-spring boundary with Liu's method. The MDRM shows its great advancement in computational accuracy, stability and range of applications. This paper also discusses the influence of boundary location on computational accuracy. It can be concluded that smaller models tend to have larger errors. By introducing two dimensionless parameters, φ_1 and φ_2, the rational distance between the observation point and the MDRM boundary is suggested. When φ_1 >2 or φ_2>13, the relative PGA error can be limited to 5%. In practice, the appropriate model size can be chosen based on these two parameters to achieve desired computational accuracy.
基金Ministry of Science and Technology of China Under Grant No.SLDRCE10-B-04the National Natural Science Foundation Under Grant No.50621062
文摘Wind loading is one of the most important loads for controlling the design of large-span roof structures. Equivalent static wind loads, which can generally aim at determining a specific response, are widely used by structural designers. A method for equivalent static wind loads applicable to multi-responses is proposed in this paper. A modified load- response-correlation （LRC） method corresponding to a particular peak response is presented, and the similarity algorithm implemented for the group response is described. The main idea of the algorithm is that two responses can be put into one group if the value of one response is close to that of the other response, when the structure is subjected to equivalent static wind loads aiming at the other response. Based on the modified LRC, the grouping response method is put forward to construct equivalent static wind loading. This technique can simultaneously reproduce peak responses for some grouped responses. To verify its computational accuracy, the method is applied to an actual large-span roof structure. Calculation results show that when the similarity of responses in the same group is high, equivalent static wind loads with high accuracy and reasonable magnitude of equivalent static wind distribution can be achieved.
文摘The density of Ni-AI alloys in both liquid state and solid-liquid coexistence state was measured with a modified pycnometric method. It was found that the density of Ni-AI alloys decreases with increasing temperature and Al concentration in the alloys. The molar volume of liquid Ni-AI binary alloys increases with the increase of temperature and Al concentration. The partial molar volume of Al in Ni-AI binary alloy was calculated approximately. The molar volume of liquid Ni-AI alloy determined in the present work shows a negative deviation from the ideal linear molar volume.
文摘In actual power systems,most of the high-voltage buses of the transformers are zero injection buses without load or generation.Power injections into these buses are strictly 0,so based on Kirchhoff's current law(KCL),equality constraints should be used to handle these buses in a state estimation model.It is a challenge to ensure that these zero injection constraints can be strictly satisfied without losing computational efficiency.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.51639002 and 51379118)Shandong University of Science and Technology Scientific Found(Grant No.2015TDJH104)
文摘The modified suction caisson(MSC) adds a short-skirted structure around the regular suction caissons to increase the lateral bearing capacity and limit the deflection. The MSC is suitable for acting as the offshore wind turbine foundation subjected to larger lateral loads compared with the imposed vertical loads. Determination of the lateral bearing capacity is a key issue for the MSC design. The formula estimating the lateral bearing capacity of the MSC was proposed in terms of the limit equilibrium method and was verified by the test results. Parametric studies on the lateral bearing capacity were also carried out. It was found that the lateral bearing capacity of the MSC increases with the increasing length and radius of the external skirt, and the lateral bearing capacity increases linearly with the increasing coefficient of subgrade reaction. The maximum lateral bearing capacity of the MSC is attained when the ratio of the radii of the internal compartment to the external skirt equals 0.82 and the ratio of the lengths of the external skirt to the internal compartment equals 0.48, provided that the steel usage of the MSC is kept constant.
文摘The density of liquid binary Ni-Mo alloys with molybdenum concentration from 0 to 20% (mass fraction) wasmeasured by a modified sessile drop method. It has been found that the density of the liquid Ni-Mo alloys decreaseswith increasing temperature, but increases with the increase of molybdenum concentration in the alloys. The molarvolume of liquid Ni-Mo binary alloys increases with the increase of temperature and molybdenum concentration. Thepartial molar volume of molybdenum in Ni-Mo binary alloy has been approximately calculated as [13.18-2.65×10^(-3)T+(-47.94+3.10×10^(-2)T)×10^(-2)X_(Mo)]×10^(-6)m^3·mol^(-1). The molar volume of Ni-Mo alloy determined inthe present work shows a negative deviation from the ideal linear mixing molar volume.
基金The authors gratefully acknowledge the financial support provided by the National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant 2016YFB0200605)the National Natural Science Foundation of China(Grant 11372004).
文摘The stiffness spreading method (SSM) was initially proposed for layout optimization of truss structures,in which an artificial elastic material of low modulus is uniformly distributed in the design domain to create connections between discrete members.In this paper,a modified stiffness spreading method is proposed by replacing the artificial elastic material with auxiliary bars to connect real members of the truss structure.Since the background continuum mesh for the elastic material is no longer required,the computational cost is significantly reduced.Like SSM,the new method is advantageous in that an initial design may consist of disconnected bars allocated in the design domain,and mathematical programming methods can be applied for the efficient solution of the formulated optimization problem.A number of solution strategies are also developed to achieve more practical designs with lower computational cost.Numerical examples of both 2-D and 3-D truss structures are presented to demonstrate the feasibility,robustness and effectiveness of the proposed method.
基金Project supported by the National Natural Science Foundation of China (Grant No 10672053)
文摘By introducing a more general auxiliary ordinary differential equation （ODE）, a modified variable separated ODE method is developed for solving the mKdV-sinh-Gordon equation. As a result, many explicit and exact solutions including some new formal solutions are successfully picked up for the mKdV-sinh-Gordon equation by this approach.
文摘The integrated structure parts are widely used in aircraft. The distortion caused by residual stresses in thick pre-stretched aluminum plates during machining integrated parts is a common and serious problem. To predict and control the machining distortion, the residual stress distribution in the thick plate must be measured firstly. The modified removal method for measuring residual stress in thick pre-stretched aluminum plates is proposed and the stress-strain relation matrix is deduced by elasticity theory. The residual stress distribution in specimen of 7050T7451 plate is measured by using the method, and measurement results are analyzed and compared with data obtained by other methods. The method is effective to measure the residual stress.
基金the Major State Basic Research Program of China un der contract No.G1999043805.
文摘On the basis of hydrographic data obtained in August 2000 cruise, the circulation in the South China Sea (SCS) is computed by the modified inverse method in combination with SSH data from TOPEX/ERS-2 analysis. For study of the dynamical mechanism, which causes the pattern of summer circulation in the SCS, the diagnostic model (Yuan et al. 1982. Acta Oceanologica Sinica,4(1):1-11; Yuan and Su. 1992. Numerical Computation of Physical Oceanography.474-542) is used to simulate numerically the summer circulation in the SCS. The following results have been obtained. (1) The central and southwestern SCSs are dominated mainly by anticy-clonic circulation systems. They are mainly as follows. 1) There is strong anticyclonic eddy southeast of Vietnam (W1). Its horizontal scale is about 300 km, and it extends vertically from the surface to the about 1 000 m level. 2) There are a warm eddy W2 southeast of Zhongsha Islands and the anticyclonic circulation system W3 west off the Luzon Island. 3) There is a stronger cyclonic eddy C1 between the anticyclonic eddies W1 and W2.4) A strong northward coastal jet is present near the coast of Vietnam, and separates from the coast of Vietnam at about 12° N to the northeast.(2)The northern SCS is dominated mainly by a cyclonic circulation system. There is a cyclonic circulation system near and north of Section N2. (3) The southeastern SCS is dominated mainly by the cyclonic circulation system. (4) Comparing the results of circulation in the SCS during the summer of 2000 with those during the summer of 1998, it is found that they agree qualitatively, but there is the some difference between them in quantity.This shows that the circulation in the SCS has obviously seasonal feature. (5) The dynamical mechanism which products the basic pattern of summer circulation is because the following two reasons: 1) the joint effect of the baroclinity and relief (JEBAR) is essential dynamical cause; and 2) it is next important dynamical cause that the interaction between the wind stress and bottom topography under the southerly monsoon. (6) Comparing the hydrographic structure and distribution of stream functions with the SSH data from TOPEX/ERS-2 analysis in the SCS during August of 2000, they agree qualitatively.
基金supported by the National Natural Science Foundation of China(Grants11632008 and 11872189)
文摘A modified slow-fast analysis method is presented for the periodically excited non-autonomous dynamical system with an order gap between the exciting frequency and the natural frequency.By regarding the exciting term as a slow-varying parameter,a generalized autonomous fast subsystem can be defined,the equilibrium branches as well as the bifurcations of which can be employed to account for the mechanism of the bursting oscillations by combining the transformed phase portrait introduced.As an example,a typical periodically excited Hartley model is used to demonstrate the validness of the method,in which the exciting frequency is far less than the natural frequency.The equilibrium branches and their bifurcations of the fast subsystem with the variation of the slow-varying parameter are presented.Bursting oscillations for two typical cases are considered,which reveals that,fold bifurcation may cause the the trajectory to jump between different equilibrium branches,while Hopf bifurcation may cause the trajectory to oscillate around the stable limit cycle.
