The effective and translational strategy to regenerate knee meniscal fibrocartilage remained challenging.Herein,we first identified vascular smooth muscle cells(VSMCs)transdifferentiated into fibrochondrocytes and par...The effective and translational strategy to regenerate knee meniscal fibrocartilage remained challenging.Herein,we first identified vascular smooth muscle cells(VSMCs)transdifferentiated into fibrochondrocytes and participated in spontaneous meniscal regeneration using smooth muscle cell lineage tracing transgenic mice meniscal defect model.Then,we identified low-intensity pulsed ultrasound(LIPUS)acoustic stimulus enhanced fibrochondrogenic transdifferentiation of VSMCs in vitro and in vivo.Mechanistically,LIPUS stimulus could up-regulate mechanosensitive ion channel Piezo1 expression and then activate the transforming growth factorβ1(TGFβ1)signal,following repression of the Notch signal,consequently enhancing fibrochondrogenic transdifferentiation of VSMCs.Finally,we demonstrated that the regular LIPUS stimulus enhanced anisotropic native-like meniscal fibrocartilage tissue regeneration in a beagle canine subtotal meniscectomy model at 6 months postoperatively.The single-cell RNA sequencing analysis confirmed the role of VSMC fibrochondrogenic transdifferentiation in meniscal regeneration.展开更多
基金supported by the National Natural Science Foundation of China(no.82172420)China Postdoctoral Science Foundation(2022M710260)Peking University Medicine Sailing Program for Young Scholars’Scientific&Technological Innovation(BMU2023YFJHMX008 and BMU2023YFJHPY023).
文摘The effective and translational strategy to regenerate knee meniscal fibrocartilage remained challenging.Herein,we first identified vascular smooth muscle cells(VSMCs)transdifferentiated into fibrochondrocytes and participated in spontaneous meniscal regeneration using smooth muscle cell lineage tracing transgenic mice meniscal defect model.Then,we identified low-intensity pulsed ultrasound(LIPUS)acoustic stimulus enhanced fibrochondrogenic transdifferentiation of VSMCs in vitro and in vivo.Mechanistically,LIPUS stimulus could up-regulate mechanosensitive ion channel Piezo1 expression and then activate the transforming growth factorβ1(TGFβ1)signal,following repression of the Notch signal,consequently enhancing fibrochondrogenic transdifferentiation of VSMCs.Finally,we demonstrated that the regular LIPUS stimulus enhanced anisotropic native-like meniscal fibrocartilage tissue regeneration in a beagle canine subtotal meniscectomy model at 6 months postoperatively.The single-cell RNA sequencing analysis confirmed the role of VSMC fibrochondrogenic transdifferentiation in meniscal regeneration.
