Neural organoids are emerging as promising in vitro models,offering a unique platform to partially recapitulate the structural and functional complexity of the human nervous system.These three-dimensional(3D)construct...Neural organoids are emerging as promising in vitro models,offering a unique platform to partially recapitulate the structural and functional complexity of the human nervous system.These three-dimensional(3D)constructs,which mimic key aspects of organ architecture,can be reliably derived from pluripotent stem cells(iPSCs)or embryonic stem cells(ESCs).Their ability to faithfully model neural development and disease pathogenesis has positioned them as indispensable tools in neuroscience research.However,to further unleash their potential,there is a pressing need for long-term and stable monitoring of their dynamic functions in a 3D context.This review provides a brief overview on diverse types of neural organoids and their induction protocols.We further highlight recent advancements in bioelectronic interfaces and sensors tailored for 3D culture.Finally,we discuss future directions aimed at advanced methodologies for real-time,multidimensional functional analysis,ultimately paving the way for breakthroughs in understanding neural development and pathology.展开更多
基金supported by the National Natural Science Foundation of China(No.82330064,32250008,62271443)Zhejiang Provincial Natural Science Foundation of China(No.LQ24H090008)+1 种基金Key Project of Zhejiang Province(2024C03146,2023C03104)the Fundamental Research Funds for the Central Universities(226-2024-00059).
文摘Neural organoids are emerging as promising in vitro models,offering a unique platform to partially recapitulate the structural and functional complexity of the human nervous system.These three-dimensional(3D)constructs,which mimic key aspects of organ architecture,can be reliably derived from pluripotent stem cells(iPSCs)or embryonic stem cells(ESCs).Their ability to faithfully model neural development and disease pathogenesis has positioned them as indispensable tools in neuroscience research.However,to further unleash their potential,there is a pressing need for long-term and stable monitoring of their dynamic functions in a 3D context.This review provides a brief overview on diverse types of neural organoids and their induction protocols.We further highlight recent advancements in bioelectronic interfaces and sensors tailored for 3D culture.Finally,we discuss future directions aimed at advanced methodologies for real-time,multidimensional functional analysis,ultimately paving the way for breakthroughs in understanding neural development and pathology.
