Objective To observe the effects of Huashi Tongbi Formula(HTF)on blood lipid levels,endothelial cell function,and gut microbiota in rats with erectile dysfunction(ED).Methods SD rats were randomly divided into blank g...Objective To observe the effects of Huashi Tongbi Formula(HTF)on blood lipid levels,endothelial cell function,and gut microbiota in rats with erectile dysfunction(ED).Methods SD rats were randomly divided into blank group,sham operation group,model group,sildenafil group,and HTF low,medium,and high dose groups,with 7 rats in each group.Hyperlipidemia combined with bilateral internal iliac artery ligation were used to establish ED model.After modeling,the sildenafil group received sildenafil(4.5 mg/kg)by gavage,while the HTF group received low,medium,and high doses(3.24,6.48,12.96 g/kg)of HTF by gavage.The other three groups received an equal volume of physiological saline by gavage.After 6weeks of intervention,the intracavernous pressure(ICP)of the penis and mean carotid artery pressure(MAP)were measured using a multi-channel physiological signal recorder,and the ICP/MAP were calculated,the number of erections in rats was recorded.Serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDLC)were measured using a fully automated biochemical analyzer.ELISA was used to detect the levels of vascular endothelial growth factor(VEGF),endothelin-1(ET-1)in serum,and microplate method was used to detect the level of nitric oxide(NO)in the corpus cavernosum tissue of rats.16 S rDNA sequencing was used to detect the gut microbiota of rats.Results Compared with baseline,the serum LDL-C levels of rats in each dose group of HTF decreased,HDL-C levels increased(P<0.05),the TC levels of rats in the medium dose group of HTF decreased(P<0.05).Compared with the blank group,the model group showed a decrease in number of erections,ICP/MAP,serum HDL-C,and NO levels in the corpus cavernosum tissue of the penis,while serum TC,LDL-C,VEGF,and ET-1 levels increased(P<0.05).Compared with the sham operation group,the model group showed a decrease in number of erections and an increase in serum VEGF and ET-1 levels(P<0.05).Compared with the model group,the sildenafil group showed a decrease in serum ET-1 levels and an increase in NO levels in the corpus cavernosum tissue of the penis(P<0.05).The levels of serum LDL-C,VEGF,and ET-1 in rats of various doses of HTF decreased,while the level of HDL-C increased(P<0.05).The low dose group of HTF had an increase in the level of NO in the corpus cavernosum tissue of the penis,while the medium dose group of HTF had an increase in the number of erections and a decrease in serum TC levels(P<0.05).Compared with the sildenafil group,the serum LDL-C and VEGF levels of rats in each dose group of HTF decreased,the HDL-C levels of rats in the low and high dose groups of HTF increased,and the ET-1 levels of rats in the low dose group of HTF decreased(P<0.05).Analysis of gut microbiota showed that HTF could affect the structure of gut microbiota.Compared with the blank group,the abundance of Butyricicoccus,Ruminococcus,Prevotellaceae_UCG-OOl,and Anaerotruncus increased in the model group,while the abundance of Candidatus_Stoquefichus,Facklamia,Jeotgalicoccus,and Enterococcus decreased(P<0.05).Compared with the model group,the HTF mediumdose groupSshowedan increase inthe abundance of Staphylococcus,Faecalibacterium,Jeotgalicoccus,Halomonas,Negativibacillus,Achromobacter,Subdoliguranulus,Lachnospiraceae UCG-010,Bilophila,and Nosocomicoccus(P<0.05),while Faecalibacterium,Family_XIll_UCG-001,and Lachnospiraceae_UCG-006,Butyricicoccus,unidentified_Ruminococcaceae,Lachnospiraceae_ND3007_group,and Lactococcus decreased(P<0.05).Conclusion The HTF may improve lipid disorders and vascular endothelial cell function in rats via affecting the abundance and diversity of gut microbiota,thereby to enhance erectile function.展开更多
Improving the computational efficiency of multi-physics simulation and constructing a real-time online simulation method is an important way to realise the virtual-real fusion of entities and data of power equipment w...Improving the computational efficiency of multi-physics simulation and constructing a real-time online simulation method is an important way to realise the virtual-real fusion of entities and data of power equipment with digital twin.In this paper,a datadriven fast calculation method for the temperature field of resin impregnated paper(RIP)bushing used in converter transformer valve-side is proposed,which combines the data dimensionality reduction technology and the surrogate model.After applying the finite element algorithm to obtain the temperature field distribution of RIP bushing under different operation conditions as the input dataset,the proper orthogonal decomposition(POD)algorithm is adopted to reduce the order and obtain the low-dimensional projection of the temperature data.On this basis,the surrogate model is used to construct the mapping relationship between the sensor monitoring data and the low-dimensional projection,so that it can achieve the fast calculation and reconstruction of temperature field distribution.The results show that this method can effectively and quickly calculate the overall temperature field distribution of the RIP bushing.The maximum relative error and the average relative error are less than 4.5%and 0.25%,respectively.The calculation speed is at the millisecond level,meeting the needs of digitalisation of power equipment.展开更多
文摘Objective To observe the effects of Huashi Tongbi Formula(HTF)on blood lipid levels,endothelial cell function,and gut microbiota in rats with erectile dysfunction(ED).Methods SD rats were randomly divided into blank group,sham operation group,model group,sildenafil group,and HTF low,medium,and high dose groups,with 7 rats in each group.Hyperlipidemia combined with bilateral internal iliac artery ligation were used to establish ED model.After modeling,the sildenafil group received sildenafil(4.5 mg/kg)by gavage,while the HTF group received low,medium,and high doses(3.24,6.48,12.96 g/kg)of HTF by gavage.The other three groups received an equal volume of physiological saline by gavage.After 6weeks of intervention,the intracavernous pressure(ICP)of the penis and mean carotid artery pressure(MAP)were measured using a multi-channel physiological signal recorder,and the ICP/MAP were calculated,the number of erections in rats was recorded.Serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDLC)were measured using a fully automated biochemical analyzer.ELISA was used to detect the levels of vascular endothelial growth factor(VEGF),endothelin-1(ET-1)in serum,and microplate method was used to detect the level of nitric oxide(NO)in the corpus cavernosum tissue of rats.16 S rDNA sequencing was used to detect the gut microbiota of rats.Results Compared with baseline,the serum LDL-C levels of rats in each dose group of HTF decreased,HDL-C levels increased(P<0.05),the TC levels of rats in the medium dose group of HTF decreased(P<0.05).Compared with the blank group,the model group showed a decrease in number of erections,ICP/MAP,serum HDL-C,and NO levels in the corpus cavernosum tissue of the penis,while serum TC,LDL-C,VEGF,and ET-1 levels increased(P<0.05).Compared with the sham operation group,the model group showed a decrease in number of erections and an increase in serum VEGF and ET-1 levels(P<0.05).Compared with the model group,the sildenafil group showed a decrease in serum ET-1 levels and an increase in NO levels in the corpus cavernosum tissue of the penis(P<0.05).The levels of serum LDL-C,VEGF,and ET-1 in rats of various doses of HTF decreased,while the level of HDL-C increased(P<0.05).The low dose group of HTF had an increase in the level of NO in the corpus cavernosum tissue of the penis,while the medium dose group of HTF had an increase in the number of erections and a decrease in serum TC levels(P<0.05).Compared with the sildenafil group,the serum LDL-C and VEGF levels of rats in each dose group of HTF decreased,the HDL-C levels of rats in the low and high dose groups of HTF increased,and the ET-1 levels of rats in the low dose group of HTF decreased(P<0.05).Analysis of gut microbiota showed that HTF could affect the structure of gut microbiota.Compared with the blank group,the abundance of Butyricicoccus,Ruminococcus,Prevotellaceae_UCG-OOl,and Anaerotruncus increased in the model group,while the abundance of Candidatus_Stoquefichus,Facklamia,Jeotgalicoccus,and Enterococcus decreased(P<0.05).Compared with the model group,the HTF mediumdose groupSshowedan increase inthe abundance of Staphylococcus,Faecalibacterium,Jeotgalicoccus,Halomonas,Negativibacillus,Achromobacter,Subdoliguranulus,Lachnospiraceae UCG-010,Bilophila,and Nosocomicoccus(P<0.05),while Faecalibacterium,Family_XIll_UCG-001,and Lachnospiraceae_UCG-006,Butyricicoccus,unidentified_Ruminococcaceae,Lachnospiraceae_ND3007_group,and Lactococcus decreased(P<0.05).Conclusion The HTF may improve lipid disorders and vascular endothelial cell function in rats via affecting the abundance and diversity of gut microbiota,thereby to enhance erectile function.
基金supported by China Postdoctoral Science Foundation,Grant 2024M753544Science and Technology Project of CSG,Grant GDKJXM2022106.
文摘Improving the computational efficiency of multi-physics simulation and constructing a real-time online simulation method is an important way to realise the virtual-real fusion of entities and data of power equipment with digital twin.In this paper,a datadriven fast calculation method for the temperature field of resin impregnated paper(RIP)bushing used in converter transformer valve-side is proposed,which combines the data dimensionality reduction technology and the surrogate model.After applying the finite element algorithm to obtain the temperature field distribution of RIP bushing under different operation conditions as the input dataset,the proper orthogonal decomposition(POD)algorithm is adopted to reduce the order and obtain the low-dimensional projection of the temperature data.On this basis,the surrogate model is used to construct the mapping relationship between the sensor monitoring data and the low-dimensional projection,so that it can achieve the fast calculation and reconstruction of temperature field distribution.The results show that this method can effectively and quickly calculate the overall temperature field distribution of the RIP bushing.The maximum relative error and the average relative error are less than 4.5%and 0.25%,respectively.The calculation speed is at the millisecond level,meeting the needs of digitalisation of power equipment.
