目的探讨急性脑梗塞(ACI)患者血清N末端B型利钠肽原(NT-proBNP)/B型利钠肽(BNP)、肌酸激酶同工酶(CK-MB)、D-二聚体(D-D)水平变化及对并发脑心综合征(CCS)的预测价值。方法纳入2020年8月至2023年8月间合肥市第八人民医院收治的ACI患者92...目的探讨急性脑梗塞(ACI)患者血清N末端B型利钠肽原(NT-proBNP)/B型利钠肽(BNP)、肌酸激酶同工酶(CK-MB)、D-二聚体(D-D)水平变化及对并发脑心综合征(CCS)的预测价值。方法纳入2020年8月至2023年8月间合肥市第八人民医院收治的ACI患者92例,根据是否并发CCS分为ACI组58例和CCS组34例,另选取同时期本院招募的健康志愿者50名为对照组。采集各组患者血液,检测NT-proBNP、BNP、CK-MB、D-D水平并计算NT-proBNP/BNP值;统计分析上述指标与ACI患者并发CCS的关系和预测价值。结果各组的NT-proBNP、BNP、NT-proBNP/BNP、CK-MB、D-D水平均有CCS组>ACI组>对照组,差异具有统计学意义(P<0.05);NT-proBNP、BNP、NT-proBNP/BNP、CK-MB、D-D、吸烟、嗜酒、高血压、冠心病、糖尿病、高血脂为发生ACI的危险因素,NT-proBNP、BNP、NT-proBNP/BNP、CK-MB、D-D、吸烟、嗜酒为ACI并发CCS的危险因素(P<0.05);NT-proBNP/BNP、CK-MB、D-D联合诊断ACI并发CCS的曲线下面积(Area under curve,AUC)最大,优于单一检测(P<0.05);NT-proBNP、BNP、NT-proBNP/BNP、CK-MB均与D-D水平正相关(P<0.05)。结论血清NT-proBNP/BNP、CK-MB、D-D水平的升高与ACI患者并发CCS相关,对ACI患者并发CSS均具有预测价值。展开更多
Introduction: A recent study in Kenya highlighted a promising advance in malaria control by demonstrating that infecting mosquitoes with the endosymbiont Microsporidia MB blocks Plasmodium transmission. However, the i...Introduction: A recent study in Kenya highlighted a promising advance in malaria control by demonstrating that infecting mosquitoes with the endosymbiont Microsporidia MB blocks Plasmodium transmission. However, the influence of biotic and abiotic factors such as diet, relative humidity (RH) and temperature on this infection remains poorly studied. This study, aimed to gain a better understanding of this relationship. Methods: To highlight the influence of diet quantity, we defined a range of 3 quantities: 0.00375 g, 0.015 g and 0.09 g. Each quantity was tested on two groups of larvae: a group of 150 larvae infected with Microsporidia MB (MB+), and a group of 150 larvae not infected with Microsporidia MB (MB−) (control group), each divided into three replicates of 50 larvae. Each replicate was fed each morning with the assigned quantity until the pupal stage. In addition to this factor, we investigated the influence of temperature and RH. We defined three temperature-RH combinations: 21˚C-80% RH, 39˚C-50% RH, and 27˚C-75% RH. Each combination was tested on two groups of larvae: a group of 150 MB+ larvae and a group of 150 MB− larvae, each divided into three replicates of 50 larvae. Each replicate was subjected to the assigned combination until pupation. Pupae that had reached the adult stage were tested by PCR to determine their Microsporidia MB infection status for each factor studied. Results: The results showed that only the lowest quantity (0.00375 g) significantly reduced the prevalence of Microsporidia MB compared with the medium quantity (chi-2 test, χ2 = 4.9088, df = 1, p = 0.02672) and the high quantity (chi-2 test, χ2 = 4.7958, df = 1, p = 0.02853). As for temperature and RH, the combination 39˚C-50% RH led to a significant reduction in the prevalence of Microsporidia MB compared with the combination 27˚C-75% RH (chi-2 test, χ2 = 6.3736, ddl = 1, p = 0.01158) and that 21˚C-80% RH (chi-2 test, χ2 = 9.983, ddl = 1, p = 0.00158). Conclusion: This work contributes to a better understanding of some key factors linked to Microsporidia MB infection in mosquitoes. However, further research on several generations is necessary to draw more comprehensive conclusions.展开更多
文摘目的探讨急性脑梗塞(ACI)患者血清N末端B型利钠肽原(NT-proBNP)/B型利钠肽(BNP)、肌酸激酶同工酶(CK-MB)、D-二聚体(D-D)水平变化及对并发脑心综合征(CCS)的预测价值。方法纳入2020年8月至2023年8月间合肥市第八人民医院收治的ACI患者92例,根据是否并发CCS分为ACI组58例和CCS组34例,另选取同时期本院招募的健康志愿者50名为对照组。采集各组患者血液,检测NT-proBNP、BNP、CK-MB、D-D水平并计算NT-proBNP/BNP值;统计分析上述指标与ACI患者并发CCS的关系和预测价值。结果各组的NT-proBNP、BNP、NT-proBNP/BNP、CK-MB、D-D水平均有CCS组>ACI组>对照组,差异具有统计学意义(P<0.05);NT-proBNP、BNP、NT-proBNP/BNP、CK-MB、D-D、吸烟、嗜酒、高血压、冠心病、糖尿病、高血脂为发生ACI的危险因素,NT-proBNP、BNP、NT-proBNP/BNP、CK-MB、D-D、吸烟、嗜酒为ACI并发CCS的危险因素(P<0.05);NT-proBNP/BNP、CK-MB、D-D联合诊断ACI并发CCS的曲线下面积(Area under curve,AUC)最大,优于单一检测(P<0.05);NT-proBNP、BNP、NT-proBNP/BNP、CK-MB均与D-D水平正相关(P<0.05)。结论血清NT-proBNP/BNP、CK-MB、D-D水平的升高与ACI患者并发CCS相关,对ACI患者并发CSS均具有预测价值。
文摘Introduction: A recent study in Kenya highlighted a promising advance in malaria control by demonstrating that infecting mosquitoes with the endosymbiont Microsporidia MB blocks Plasmodium transmission. However, the influence of biotic and abiotic factors such as diet, relative humidity (RH) and temperature on this infection remains poorly studied. This study, aimed to gain a better understanding of this relationship. Methods: To highlight the influence of diet quantity, we defined a range of 3 quantities: 0.00375 g, 0.015 g and 0.09 g. Each quantity was tested on two groups of larvae: a group of 150 larvae infected with Microsporidia MB (MB+), and a group of 150 larvae not infected with Microsporidia MB (MB−) (control group), each divided into three replicates of 50 larvae. Each replicate was fed each morning with the assigned quantity until the pupal stage. In addition to this factor, we investigated the influence of temperature and RH. We defined three temperature-RH combinations: 21˚C-80% RH, 39˚C-50% RH, and 27˚C-75% RH. Each combination was tested on two groups of larvae: a group of 150 MB+ larvae and a group of 150 MB− larvae, each divided into three replicates of 50 larvae. Each replicate was subjected to the assigned combination until pupation. Pupae that had reached the adult stage were tested by PCR to determine their Microsporidia MB infection status for each factor studied. Results: The results showed that only the lowest quantity (0.00375 g) significantly reduced the prevalence of Microsporidia MB compared with the medium quantity (chi-2 test, χ2 = 4.9088, df = 1, p = 0.02672) and the high quantity (chi-2 test, χ2 = 4.7958, df = 1, p = 0.02853). As for temperature and RH, the combination 39˚C-50% RH led to a significant reduction in the prevalence of Microsporidia MB compared with the combination 27˚C-75% RH (chi-2 test, χ2 = 6.3736, ddl = 1, p = 0.01158) and that 21˚C-80% RH (chi-2 test, χ2 = 9.983, ddl = 1, p = 0.00158). Conclusion: This work contributes to a better understanding of some key factors linked to Microsporidia MB infection in mosquitoes. However, further research on several generations is necessary to draw more comprehensive conclusions.
