基于现有的渤海浮式生产储卸油装置(Floating Production Storage and Offloading,FPSO)实测数据,开展海洋核动力平台(Marine Nuclear Power Platform,MNPP)定位系统多体动力特性分析。建立MNPP定位系统的多刚体动力学模型,通过MNPP与...基于现有的渤海浮式生产储卸油装置(Floating Production Storage and Offloading,FPSO)实测数据,开展海洋核动力平台(Marine Nuclear Power Platform,MNPP)定位系统多体动力特性分析。建立MNPP定位系统的多刚体动力学模型,通过MNPP与渤海某FPSO的响应幅值算子(Response Amplitude Operator,RAO),计算FPSO船体实测数据与MNPP响应的比例系数;根据FPSO船体实测数据计算MNPP六自由度数据,并采用经验模态分解(Empirical Mode Decomposition,EMD)方法保留原始数据的波频成分,在此基础上计算定位系统的系泊回复能力和各铰节点的受力行为。通过与FPSO软刚臂系泊系统受力状态进行对比分析,验证MNPP定位系统在实测海况下设计的合理性。可为MNPP系泊结构设计和安全运行提供科学的分析手段。展开更多
Replacement of the native heme cofactor by manganese protoporphyrinⅨ(MnPPⅨ)to reconstitute manganese myoglobin(Mn^(Ⅲ)Mb)is an important approach to investigate the reactivity of the Mn center inside protein scaffol...Replacement of the native heme cofactor by manganese protoporphyrinⅨ(MnPPⅨ)to reconstitute manganese myoglobin(Mn^(Ⅲ)Mb)is an important approach to investigate the reactivity of the Mn center inside protein scaffolds.However,unlike the Mn porphyrin synthetic model compounds,MnPPⅨreconstituted myoglobins(Mn^(Ⅲ)Mb)have no reactivity in the epoxidation of styrene using H_(2)O_(2),which was attributed to the low reactivity of the Mn^(Ⅳ)=O intermediate after homocleavage of the O-O bond in manganese peroxide.To address this issue,we herein chose Oxone^(®)(2KHSO_(5)·KHSO_(4)·K_(2)SO_(4)),a well-known oxidant undergoing O-O bond heterocleavage.After screening 7 mutants and wild-type Mn^(Ⅲ)Mb,we found that the L29H/F43H mutant could generate a new species([Mn^(Ⅳ)=O]^(+·)),tentatively assigned by using UV-vis and EPR spectra,through heterocleavage of the O-O bond.Computational docking showed hydrogen bonds between three distal histidines(H64,L29H and F43H)and anions,which increase the binding affinity to persulfate.With Oxone^(®)as the oxidant,Mn^(Ⅲ)Mb(L29H/F43H)showed the highest reactivity toward the epoxidation of styrene,different from that with the H_(2)O_(2)oxidant.This work demonstrates the first example of MnPPIX reconstituted Mb which could catalyze styrene epoxidation and provides new insights to further explore the reactivity of the Mn center in protein scaffolds.展开更多
文摘基于现有的渤海浮式生产储卸油装置(Floating Production Storage and Offloading,FPSO)实测数据,开展海洋核动力平台(Marine Nuclear Power Platform,MNPP)定位系统多体动力特性分析。建立MNPP定位系统的多刚体动力学模型,通过MNPP与渤海某FPSO的响应幅值算子(Response Amplitude Operator,RAO),计算FPSO船体实测数据与MNPP响应的比例系数;根据FPSO船体实测数据计算MNPP六自由度数据,并采用经验模态分解(Empirical Mode Decomposition,EMD)方法保留原始数据的波频成分,在此基础上计算定位系统的系泊回复能力和各铰节点的受力行为。通过与FPSO软刚臂系泊系统受力状态进行对比分析,验证MNPP定位系统在实测海况下设计的合理性。可为MNPP系泊结构设计和安全运行提供科学的分析手段。
基金supported by the National Scientific Foundation of China(grant no.20971007,21101169,21271013,and 21571007)the National Key Basic Research Support Foundation of China(NKBRSFC)(2010CB912302,2015CB856300,and 2015CB856301).
文摘Replacement of the native heme cofactor by manganese protoporphyrinⅨ(MnPPⅨ)to reconstitute manganese myoglobin(Mn^(Ⅲ)Mb)is an important approach to investigate the reactivity of the Mn center inside protein scaffolds.However,unlike the Mn porphyrin synthetic model compounds,MnPPⅨreconstituted myoglobins(Mn^(Ⅲ)Mb)have no reactivity in the epoxidation of styrene using H_(2)O_(2),which was attributed to the low reactivity of the Mn^(Ⅳ)=O intermediate after homocleavage of the O-O bond in manganese peroxide.To address this issue,we herein chose Oxone^(®)(2KHSO_(5)·KHSO_(4)·K_(2)SO_(4)),a well-known oxidant undergoing O-O bond heterocleavage.After screening 7 mutants and wild-type Mn^(Ⅲ)Mb,we found that the L29H/F43H mutant could generate a new species([Mn^(Ⅳ)=O]^(+·)),tentatively assigned by using UV-vis and EPR spectra,through heterocleavage of the O-O bond.Computational docking showed hydrogen bonds between three distal histidines(H64,L29H and F43H)and anions,which increase the binding affinity to persulfate.With Oxone^(®)as the oxidant,Mn^(Ⅲ)Mb(L29H/F43H)showed the highest reactivity toward the epoxidation of styrene,different from that with the H_(2)O_(2)oxidant.This work demonstrates the first example of MnPPIX reconstituted Mb which could catalyze styrene epoxidation and provides new insights to further explore the reactivity of the Mn center in protein scaffolds.
