Mitoehondria are involved in apoptosis of mammalian cells and even singlecell organisms, but mitochondria are not required in apoptosis in cultured Drosophila cells such as S2 and BG2 cell lines. It is not very clear ...Mitoehondria are involved in apoptosis of mammalian cells and even singlecell organisms, but mitochondria are not required in apoptosis in cultured Drosophila cells such as S2 and BG2 cell lines. It is not very clear whether mitochondria are involved in apoptosis in other insect cells such as lepidopteran cell lines. Thus, we determined to elucidate the role of mitochondria in apoptosis induced by ultraviolet radiation in Spodoptera litura （Lepidoptera： Noctuidae） cell line （SL-ZSU-1）. The Western blot results suggested that cytochrome c in the ultraviolet-treated SL-1 cells was released from the mitochondria to cytosol as early as 4 h after the induction of ultraviolet radiation and increased in the cytosolic fractions in a time-dependent manner. Flow cytometric analysis of mitochondrial membrane potential （△ ψm） of SL-ZSU-1 cell treated with ultraviolet-C （UV-C） light indicated the decrease in mitochondrial membrane potential was dependent on the times of ultraviolet treatment. Both of them are different from apoptosis in cultured Drosophila melanogaster cell lines （S2 and BG2） and it appears evident mitochondria are involved in apoptosis of the studied lepidopteran cells.展开更多
基金Acknowledgments This work was supported by the National Natural Science Foundation of China （Grant No： 30470073） and the Natural Science Foundation of Hubei Province （Grant No： 2007ABA 160）.
文摘Mitoehondria are involved in apoptosis of mammalian cells and even singlecell organisms, but mitochondria are not required in apoptosis in cultured Drosophila cells such as S2 and BG2 cell lines. It is not very clear whether mitochondria are involved in apoptosis in other insect cells such as lepidopteran cell lines. Thus, we determined to elucidate the role of mitochondria in apoptosis induced by ultraviolet radiation in Spodoptera litura （Lepidoptera： Noctuidae） cell line （SL-ZSU-1）. The Western blot results suggested that cytochrome c in the ultraviolet-treated SL-1 cells was released from the mitochondria to cytosol as early as 4 h after the induction of ultraviolet radiation and increased in the cytosolic fractions in a time-dependent manner. Flow cytometric analysis of mitochondrial membrane potential （△ ψm） of SL-ZSU-1 cell treated with ultraviolet-C （UV-C） light indicated the decrease in mitochondrial membrane potential was dependent on the times of ultraviolet treatment. Both of them are different from apoptosis in cultured Drosophila melanogaster cell lines （S2 and BG2） and it appears evident mitochondria are involved in apoptosis of the studied lepidopteran cells.
