[Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. [Method] The combined method of suppressing subtractive hybridization and cDNA micro-array wer...[Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. [Method] The combined method of suppressing subtractive hybridization and cDNA micro-array were used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition, the expression profile analysis was conducted in different organs and different mature periods of banana.[Result] The whole length of cDNA in MaPRMT1 was 1 158 bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high homology with PRMT in plant,containing one Methyltransf_1 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.[Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and fruits at different mature periods.展开更多
The arbitrarily primed polymerase chain reaction(AP-PCR)technique was used to detect polymorphisms in genomic DNA from Oryza minuta, O. officinalis, and related rice species. The results show that the genetic distance...The arbitrarily primed polymerase chain reaction(AP-PCR)technique was used to detect polymorphisms in genomic DNA from Oryza minuta, O. officinalis, and related rice species. The results show that the genetic distance between O.minuta and O.officinalis is much higher than that between two known species,O.sativa and O. rufipogon, showing that O.minuto and O.officinalis should be classified as two different spedes in genus Oryza,and that the AP-PCR technique can be used as a powerful tool for studying taxonomic relationships among related spedes, and even subspecies.展开更多
文摘[Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. [Method] The combined method of suppressing subtractive hybridization and cDNA micro-array were used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition, the expression profile analysis was conducted in different organs and different mature periods of banana.[Result] The whole length of cDNA in MaPRMT1 was 1 158 bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high homology with PRMT in plant,containing one Methyltransf_1 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.[Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and fruits at different mature periods.
文摘The arbitrarily primed polymerase chain reaction(AP-PCR)technique was used to detect polymorphisms in genomic DNA from Oryza minuta, O. officinalis, and related rice species. The results show that the genetic distance between O.minuta and O.officinalis is much higher than that between two known species,O.sativa and O. rufipogon, showing that O.minuto and O.officinalis should be classified as two different spedes in genus Oryza,and that the AP-PCR technique can be used as a powerful tool for studying taxonomic relationships among related spedes, and even subspecies.
