Photothermal therapy(PTT)is a desirable way to attain on-demand hyperthermia owing to the heat gen-erated by photothermal materials absorbing near-infrared light.The mild heat(42±0.5℃)can not only accelerate bon...Photothermal therapy(PTT)is a desirable way to attain on-demand hyperthermia owing to the heat gen-erated by photothermal materials absorbing near-infrared light.The mild heat(42±0.5℃)can not only accelerate bone tissue regeneration but also promote the release of bioactive ions from biomaterials.Based on this one-stone-two-birds strategy,a 3D printed PEEK-graphene composite scaffold(PG)with hydroxyapatite(HA)coating(PGH)for photothermally remote control of bone regeneration was well de-signed in this study.The results showed that the HA coating on PGH could release Ca^(2+)and PO_(4)^(3-)ions easily under NIR irradiation,which was ascribed to the mild heat generated from graphene in the com-posite scaffold.The mild heat and the boosted Ca^(2+)/PO_(4)^(3-)release could synergistically enhance the bone regeneration ability both in vitro and in vivo.The underlying mechanism was further explored and con-firmed to be closely related to the upregulation of HSP 70,by which the MAPK/ERK signaling pathway was activated selectively.The favorable results demonstrate that the 3D-printed PEEK/graphene composite scaffold is promising in the applications of bone defect repair.展开更多
Above-optimal temperatures reduce yield in many crops, including tomato, largely because of the heat-sensitivity of their reproduction process. A full understanding of heat-stress (HS) response and thermotolerance of ...Above-optimal temperatures reduce yield in many crops, including tomato, largely because of the heat-sensitivity of their reproduction process. A full understanding of heat-stress (HS) response and thermotolerance of tomato reproduction is still lacking. Recently, using external application of the plant <span style="font-family:Verdana;">hormone ethylene, it was demonstrated that ethylene plays a role in</span><span style="font-family:Verdana;"> heat-tolerance of tomato pollen (the male reproductive cells). In order to expand our understanding on involvement of ethylene in tomato pollen thermotolerance, </span><span style="font-family:Verdana;">we analyzed the response of wild type and ethylene-related tomato mutant</span> <span style="font-family:Verdana;">plants to HS, at physiological and molecular levels. We report that mild</span><span style="font-family:Verdana;"> chronic </span><span style="font-family:Verdana;">HS conditions highly reduce the number of viable and germinating pollen </span><span style="font-family:Verdana;">grains as well as the production of seeded fruits in wild type tomato plants, while no significant reduction was detected/observed in pollen quality, number of seeded fruits and seeds per fruit in plants of the ethylene over-producer mutant epinastic. Our findings suggest that ethylene is involved in thermotolerance of tomato reproduction, pointing to an effect on pollen viability and </span><span style="font-family:Verdana;">germination potential, highlighting candidate genes involved in pollen re</span><span style="font-family:Verdana;">sponse to HS (like </span><i><span style="font-family:Verdana;">SlHSP17</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">SlHSP101</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">SlMBF1</span></i><span style="font-family:Verdana;">) and suggesting directions for further studies.</span>展开更多
Wound healing remains a critical clinical challenge due to inflammatory responses,oxidative stress in the wound microenvironment,and impaired tissue remodeling.In this study,an anisotropic scaffold was developed by in...Wound healing remains a critical clinical challenge due to inflammatory responses,oxidative stress in the wound microenvironment,and impaired tissue remodeling.In this study,an anisotropic scaffold was developed by integrating photothermal stimulation with topographical cues to modulate wound healing.The scaffold consisted of gelatin methacryloyl(GM)hydrogel and radially aligned poly(ε-caprolactone)(PCL)fibers integrated with polydopamine(PDA).The anisotropic scaffold not only exhibited anti-inflammatory effects but also enabled localized thermal stimulation under near-infrared(NIR)light to promote wound healing.It guided cell migration and proliferation from the wound edge toward the center,while the GM hydrogel maintained a moist envi-ronment and mitigated uncontrolled thermal damage.In a full-thickness skin wound model in rats,the aniso-tropic scaffold accelerated wound healing,epidermal regeneration,angiogenesis,and collagen deposition.This approach offers a safe,efficient,and bioactive-factor-free therapeutic strategy for wound repair,showing great potential for clinical translation.展开更多
Metastatic dissemination is the major cause of death from breast-cancer(BC).Fusobacterium nucleatum(F.n)is widely enriched in BC and has recently been identified as one of the high-risk factors for promoting BC metast...Metastatic dissemination is the major cause of death from breast-cancer(BC).Fusobacterium nucleatum(F.n)is widely enriched in BC and has recently been identified as one of the high-risk factors for promoting BC metastasis.Here,with an experimental model,we demonstrated that intratumoral F.n induced BC aggressiveness by transcriptionally activating Epithelial-mesenchymal transition-associated genes.Therefore,the F.n may be a potential target to prevent metastasis.Given the fact that cancer-associated fibroblasts(CAFs)are abundant in BC and located near blood vessels,we report an optogenetic system that drives CAF to in situ produce human antibacterial peptide LL37,with the characteristics of biosafety and freely intercellular trafficking,for depleting intratumoral F.n,leading to a 72.1%reduction in lung metastatic nodules number without affecting the balance of the systemic flora.Notably,mild photothermal treatment was found that could normalize CAF,contributing to synergistically inhibiting BC metastasis.In addition,the system can also simultaneously encode a gene of TNF-related apoptosis-inducing ligand to suppress the primary tumor.Together,our study highlights the potential of local elimination of tumor pathogenic bacteria to prevent BC metastasis.展开更多
基金supported by grants from the Project funded by the China Postdoctoral Science Foundation(Nos.2023M732469 and 2023M732477)the Sichuan University Postdoctoral Interdisciplinary Innovation Fund(No.JCXK2205)+3 种基金the Projects of the Science and Technology Department of Sichuan Province(Nos.24NSFSC7945,24NSFSC6493,2022ZDZX0029,2023NSFSC0659,MZGC20230019)the National Natural Science Foundation of China(No.52302351)the Sichuan Provincial Medical Association Special Research Fund(Nos.2021SAT05 and 2019HR18)the National Program for Postdoctoral Researchers(No.GZB20230485)as well as the Fundamental Research Funds for the Central Universities(No.2023SCU12116).
文摘Photothermal therapy(PTT)is a desirable way to attain on-demand hyperthermia owing to the heat gen-erated by photothermal materials absorbing near-infrared light.The mild heat(42±0.5℃)can not only accelerate bone tissue regeneration but also promote the release of bioactive ions from biomaterials.Based on this one-stone-two-birds strategy,a 3D printed PEEK-graphene composite scaffold(PG)with hydroxyapatite(HA)coating(PGH)for photothermally remote control of bone regeneration was well de-signed in this study.The results showed that the HA coating on PGH could release Ca^(2+)and PO_(4)^(3-)ions easily under NIR irradiation,which was ascribed to the mild heat generated from graphene in the com-posite scaffold.The mild heat and the boosted Ca^(2+)/PO_(4)^(3-)release could synergistically enhance the bone regeneration ability both in vitro and in vivo.The underlying mechanism was further explored and con-firmed to be closely related to the upregulation of HSP 70,by which the MAPK/ERK signaling pathway was activated selectively.The favorable results demonstrate that the 3D-printed PEEK/graphene composite scaffold is promising in the applications of bone defect repair.
文摘Above-optimal temperatures reduce yield in many crops, including tomato, largely because of the heat-sensitivity of their reproduction process. A full understanding of heat-stress (HS) response and thermotolerance of tomato reproduction is still lacking. Recently, using external application of the plant <span style="font-family:Verdana;">hormone ethylene, it was demonstrated that ethylene plays a role in</span><span style="font-family:Verdana;"> heat-tolerance of tomato pollen (the male reproductive cells). In order to expand our understanding on involvement of ethylene in tomato pollen thermotolerance, </span><span style="font-family:Verdana;">we analyzed the response of wild type and ethylene-related tomato mutant</span> <span style="font-family:Verdana;">plants to HS, at physiological and molecular levels. We report that mild</span><span style="font-family:Verdana;"> chronic </span><span style="font-family:Verdana;">HS conditions highly reduce the number of viable and germinating pollen </span><span style="font-family:Verdana;">grains as well as the production of seeded fruits in wild type tomato plants, while no significant reduction was detected/observed in pollen quality, number of seeded fruits and seeds per fruit in plants of the ethylene over-producer mutant epinastic. Our findings suggest that ethylene is involved in thermotolerance of tomato reproduction, pointing to an effect on pollen viability and </span><span style="font-family:Verdana;">germination potential, highlighting candidate genes involved in pollen re</span><span style="font-family:Verdana;">sponse to HS (like </span><i><span style="font-family:Verdana;">SlHSP17</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">SlHSP101</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">SlMBF1</span></i><span style="font-family:Verdana;">) and suggesting directions for further studies.</span>
基金funded by National Key R&D Program of China(Grant No.2022YFC2408200 and 2022YFC3006204)the National Natural Science Foundation of China(Grant No.52221006,52473121,82302793 and 52403370)Fundamental Research Funds for the Central Universities(buctrc202020,buctrc202312).
文摘Wound healing remains a critical clinical challenge due to inflammatory responses,oxidative stress in the wound microenvironment,and impaired tissue remodeling.In this study,an anisotropic scaffold was developed by integrating photothermal stimulation with topographical cues to modulate wound healing.The scaffold consisted of gelatin methacryloyl(GM)hydrogel and radially aligned poly(ε-caprolactone)(PCL)fibers integrated with polydopamine(PDA).The anisotropic scaffold not only exhibited anti-inflammatory effects but also enabled localized thermal stimulation under near-infrared(NIR)light to promote wound healing.It guided cell migration and proliferation from the wound edge toward the center,while the GM hydrogel maintained a moist envi-ronment and mitigated uncontrolled thermal damage.In a full-thickness skin wound model in rats,the aniso-tropic scaffold accelerated wound healing,epidermal regeneration,angiogenesis,and collagen deposition.This approach offers a safe,efficient,and bioactive-factor-free therapeutic strategy for wound repair,showing great potential for clinical translation.
基金supported by the National Natural Science Foundation of China(82172762,21904119,and 82073395)Science and technology innovation talents support project of Henan Province(23HASTIT041)Henan Science Fund for Outstanding Young Scholars(232300421051,China).
文摘Metastatic dissemination is the major cause of death from breast-cancer(BC).Fusobacterium nucleatum(F.n)is widely enriched in BC and has recently been identified as one of the high-risk factors for promoting BC metastasis.Here,with an experimental model,we demonstrated that intratumoral F.n induced BC aggressiveness by transcriptionally activating Epithelial-mesenchymal transition-associated genes.Therefore,the F.n may be a potential target to prevent metastasis.Given the fact that cancer-associated fibroblasts(CAFs)are abundant in BC and located near blood vessels,we report an optogenetic system that drives CAF to in situ produce human antibacterial peptide LL37,with the characteristics of biosafety and freely intercellular trafficking,for depleting intratumoral F.n,leading to a 72.1%reduction in lung metastatic nodules number without affecting the balance of the systemic flora.Notably,mild photothermal treatment was found that could normalize CAF,contributing to synergistically inhibiting BC metastasis.In addition,the system can also simultaneously encode a gene of TNF-related apoptosis-inducing ligand to suppress the primary tumor.Together,our study highlights the potential of local elimination of tumor pathogenic bacteria to prevent BC metastasis.
