In the context of the rapid development of artificial intelligence and robotics,their application scenarios are continuously expanding to a variety of complex environments,with increasing attention being paid to the u...In the context of the rapid development of artificial intelligence and robotics,their application scenarios are continuously expanding to a variety of complex environments,with increasing attention being paid to the use of flexible sensors in lowtemperature environments.In this study,an ionic hydrogel was synthesized using acrylamide(AM),hydroxyethyl cellulose(HEC),and lithium chloride(LiCl)as composites.This hydrogel exhibits high adhesion,excellent sensitivity(gauge factor(GF)=2.84),rapid response time(100 ms),exceptional stretch ability(>1776%),high toughness(2.5 MJ/m^(3)),and the ability to maintain detectability at low temperatures(-60℃).HEC imparts reliable mechanical properties to the sensor through hydrogen bonding interactions of its hydroxyl groups.LiCl ensures that the sensor has outstanding antifreezing properties,maintains good conductivity and mechanical performance.Used for robotic attitude detection,the sensor demonstrated accurate recognition of various joint movements at both 20 and -20℃.This technology was extended to industrial operations and maintenance,where a mechanical claw was used to grasp parts at both room temperature and low temperature.A convolutional neural network deep learning algorithm was employed to identify and classify eight types of parts,achieving an impressive recognition accuracy of 98.8%.The polyacrylamide(PAM)/HEC/LiCl hydrogel sensor demonstrates the capability for wide-temperature range detection in flexible robotics,holding significant potential for future applications in human-machine interaction,tactile perception,and related fields.展开更多
The published article titled“MicroRNA-133b Inhibits Proliferation,Cellular Migration,and Invasion via Targeting LASP1 in Hepatocarcinoma Cells”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1269–1282.
Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postn...Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postnatal neurogenesis remains unclear.In this study,to define the precise role of transforming growth factor-βsignaling in postnatal neurogenesis at distinct stages of the neurogenic cascade both in vitro and in vivo,we developed two novel inducible and cell type-specific mouse models to specifically silence transforming growth factor-βsignaling in neural stem cells in(mGFAPcre-ALK5fl/fl-Ai9)or immature neuroblasts in(DCXcreERT2-ALK5fl/fl-Ai9).Our data showed that exogenous transforming growth factor-βtreatment led to inhibition of the proliferation of primary neural stem cells while stimulating their migration.These effects were abolished in activin-like kinase 5(ALK5)knockout primary neural stem cells.Consistent with this,inhibition of transforming growth factor-βsignaling with SB-431542 in wild-type neural stem cells stimulated proliferation while inhibited the migration of neural stem cells.Interestingly,deletion of transforming growth factor-βreceptor in neural stem cells in vivo inhibited the migration of postnatal born neurons in mGFAPcre-ALK5fl/fl-Ai9 mice,while abolishment of transforming growth factor-βsignaling in immature neuroblasts in DCXcreERT2-ALK5fl/fl-Ai9 mice did not affect the migration of these cells in the hippocampus.In summary,our data supports a dual role of transforming growth factor-βsignaling in the proliferation and migration of neural stem cells in vitro.Moreover,our data provides novel insights on cell type-specific-dependent requirements of transforming growth factor-βsignaling on neural stem cell proliferation and migration in vivo.展开更多
In wave-equation migration and demigration,the cross-correlation imaging/forwarding step implicitly injects an additional copy of the source wavelet,so that the amplitude spectrum of the wavelet is applied redundantly...In wave-equation migration and demigration,the cross-correlation imaging/forwarding step implicitly injects an additional copy of the source wavelet,so that the amplitude spectrum of the wavelet is applied redundantly(effectively imposing a wavelet-spectrum weighting,often akin to an amplitude-squared bias).This redundancy degrades structural fidelity and amplitude balance yet is frequently overlooked.We(i)formalize the mechanism by which cross-correlation duplicates the source-wavelet amplitude effect in both migration and demigration,and(ii)introduce a source-equalized operator that removes the redundancy by deconvolving(or dividing by)the wavelet amplitude spectrum in the imaging condition and its demigration counterpart,while leaving phase/kinematics intact.Using a band-limited Ricker wavelet on a two-layer model and on Marmousi,we show that,if unmanaged,the redundant wavelet spectrum broadens main lobes,introduces ringing,and suppresses vertical resolution in migrated images,and inflates spectrum mismatches between demigrated and observed data even when peak times agree.With our correction,images recover observed-data-consistent bandwidth and sharpened interfaces,and demigrated data also exhibit improved spectrum conformity and reduced amplitude misfit.The results clarify when source amplitudes matter,why cross-correlation makes them redundantly matter,and how a lightweight spectral correction restores physically meaningful amplitude behavior in wave-equation migration/demigration.展开更多
Summary： In order to study the effects of exogenous sonic hedgehog （shh） peptide on proliferation, adhesion, migration of endothelial progenitor cells （EPCs） from rat peripheral blood, the mononuclear cells were ...Summary： In order to study the effects of exogenous sonic hedgehog （shh） peptide on proliferation, adhesion, migration of endothelial progenitor cells （EPCs） from rat peripheral blood, the mononuclear cells were collected from rat peripheral blood by Ficoll density gradient centrifugation. EPCs were iso- lated with adherence screening method and cultured in M199 culture medium with the supplement of VEGF and bFGF. The immunohistochemical staining was used to identify cell markers such as CD133 and VEGFR-2. EPCs were stimulated with exogenous shh peptide of different final concentrations （0.01, 0.1, 1, 10 μg/mL）. The proliferation, adhesion and migration of EPCs were detected by MTT chromom- etry, adhesion test and transwell system, respectively. The results of this study showed that, after 7 days of culture, cells formed clusters, assuming typical cobbles-tone pattern under microscope. After 2 weeks of culture, cells were arranged in cord-like fashion and sometimes grew like ＂micro-vessels＂. Immuno- histochemical staining showed that the cultured cells were positive for both CD133 and VEGFR-2. The proliferation, adhesion and migration of EPCs could be promoted by endogenous shh peptide at concen- trations from 0.1 μg/mL to 10 μg/mL in a concentration-dependent manner. The findings indicate that exogenous shh peptide can enhance EPCs proliferation, adhesion, and migration, which may have a po- tential value for clinical application.展开更多
Objective: To discuss the ef ect of BRMS1 on the proliferation, migration and adhesion of mouse forestomach carcinoma(MFC). Methods: The constructed p CMV-myc-BRMS1 recombinant plasmid and blank plasmid were transfect...Objective: To discuss the ef ect of BRMS1 on the proliferation, migration and adhesion of mouse forestomach carcinoma(MFC). Methods: The constructed p CMV-myc-BRMS1 recombinant plasmid and blank plasmid were transfected into mouse forestomach carcinoma. MTT method was employed to measure the activity of gastric cancer cell; the scratch assay and Transwell assay to measure the migration and invasion of gastric cancer cell; the adhesion assay to measure the adhesion of gastric cancer cell; while the Western blot assay to measure the expression of The NF-毷B signal pathway, downstream matrix metalloproteinase(MMP-2), MMP-9 and osteopontin and E-cadherin in the gastric cancer cell. Besides, the transplanted animal model of gastric cancer in mice was constructed to measure the size of tumor xenograft. Results: Results of MTT assay showed that, compared with the empty vector control group, the activity of gastric cancer cell was not af ected in the BRMS1 transfection group. The improved expression of BRMS1 could inhibit the adhesion, migration and invasion of gastric cancer cell(P<0.01). Besides, compared with the empty vector control group, the phosphorylation of NF-毷B p65 and I毷Bα was reduced in the BRMS1 transfection group, with the decreased expression of MMP 2, MMP 9 and osteopontin and the increased expression of E-cadherin(P<0.01). Results of animal experiment also showed that the expression of BRMS1 did not af ect the transplanted tumor. Conclusions: The expression of BRMS1 can signii cantly inhibit the adhesion, migration, invasion and metastasis of MCF gastric cancer cell, which is related to The NF-毷B signal pathway.展开更多
It has been reported that metastasis-associated gene 1 （Mta1） is overexpressed in many malignant tumors with high metastatic potential. In addition, some studies indicated that MTA1 participated in invasion, metasta...It has been reported that metastasis-associated gene 1 （Mta1） is overexpressed in many malignant tumors with high metastatic potential. In addition, some studies indicated that MTA1 participated in invasion, metastasis, and survival of cancer cells by regulating cell migration, adhesion and proliferation. But the role of MTA1 is unclear in vitro in the development of cervical cancer cells. This study investigated whether and how MTA1 mediated cell proliferation, migration, invasion and adhesion in cervical cancer. MTA1 expression level was detected by Western blot in two cervical cancer cell lines of different invasion potentials. The effects of MTA1 expression on SiHa cell apoptosis, cycle, proliferation, migration, invasion and adhesion were tested by flow cytometry, MTT, wound-healing assay, Transwell assay and adhesion assay, respectively. The expression levels of p53, E-cadherin, and β-catenin activity were evaluated in untreated and treated cells. The results showed that MTA1 protein expression was significantly higher in SiHa than in HeLa, which was correlated well with the potential of migration and invasion in both cell lines. Furthermore, the cell invasion, migration and adhesion capabilities were decreased after inhibition of MTA1 expression mediated by Mta1-siRNA transfection in SiHa. However, no significant differences were found in cell apoptosis, cycle, and proliferation. In addition, E-cadherin and p53 protein levels were significantly up-regulated, while β-catenin was significantly down-regulated in SiHa transfected with the siRNA. These results demonstrated that MTA1 played an important role in the migration and invasion of cervical cancer cells. It was speculated that the decreased migration and invasion capability by inhibiting the MTA1 expression in the SiHa cell line may be mediated through the altered expression of p53, and E-cadherin/β-catenin complex. MTA1 could serve as a potential therapeutic target in cervical cancer.展开更多
Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration vi...Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.展开更多
AIM： To investigate the relationship between Interleu- kin-8 （IL-8） and proliferation, adhesion, migration, inva- sion and chemosensitivity of gastric cancer （GC） cells.
Transforming growth factor-b 1 (TGF-β1), a multi-function polypeptide, is a double-edged sword in cancer. For some tumor cells, TGF-β1 is a potent growth inhibitor and apoptosis inducer. More commonly, TGF-β1 loses...Transforming growth factor-b 1 (TGF-β1), a multi-function polypeptide, is a double-edged sword in cancer. For some tumor cells, TGF-β1 is a potent growth inhibitor and apoptosis inducer. More commonly, TGF-β1 loses its growth-inhibitory and apoptosis-inducing effects, but stimulates the metastatic capacity of tumor cells. It is currently little known about TGF-β1-promoted cell migration in hepatocellular carcinoma (HCC) cells, let alone its mechanism. In this study, we found that TGF-β1 lost its tumor-suppressive effects, but significantly stimulated cell migration in SMMC-7721 human HCC cells. By FACS and Western blot analysis, we observed that TGF-β1 enhanced the expression of α5β1 integrin obviously, and subsequently stimulated cell adhesion onto fibronectin (Fn). Furthermore, we observed that TGF-β1 could also promote SMMC-7721 cells adhesion onto laminin (Ln). Our data also provided evidences that TGF-β1 induced epithelial-to-mesenchymal transformation (EMT) in SMMC-7721 cells. First, SMMC-7721 cells clearly switched to the spindle shape morphology after TGF-β1 treatment. Furthermore, TGF-β1 induced the down-regulation of E-cadherin and the nuclear translocation of β-catenin. These results indicated that TGF-β1-promoted cell adhesion and TGF-β1-induced epithelial-to-mesenchymal transformation might be both responsible for TGF-β1-enhanced cell migration.展开更多
Cell adhesion and migration are basic physiolog- ical processes in living organisms. Cells can actively probe their mechanical micro-environment and respond to the ex- ternal stimuli through cell adhesion. Cells need ...Cell adhesion and migration are basic physiolog- ical processes in living organisms. Cells can actively probe their mechanical micro-environment and respond to the ex- ternal stimuli through cell adhesion. Cells need to move to the targeting place to perform function via cell migration. For adherent cells, cell migration is mediated by cell-matrix adhesion and cell-cell adhesion. Experimental approaches, especially at early stage of investigation, are indispensable to studies of cell mechanics when even qualitative behaviors of cell as well as fundamental factors in cell behaviors are unclear. Currently, there is increasingly accumulation of ex- perimental data of measurement, thus a quantitative formula- tion of cell behaviors and the relationship among these fun- damental factors are highly needed. This quantitative under- standing should be crucial to tissue engineering and biomed- ical engineering when people want to accurately regulate or control cell behaviors from single cell level to tissue level. In this review, we will elaborate recent advances in the ex- perimental and theoretical studies on cell adhesion and mi- gration, with particular focuses laid on recent advances in experimental techniques and theoretical modeling, through which challenging problems in the cell mechanics are sug- gested.展开更多
The surfaces and interfaces of biomaterials interact with the biological systems in multi-scale levels,and thereby influence the biological functions and comprehensive performance in vitro and in vivo.In particular,a ...The surfaces and interfaces of biomaterials interact with the biological systems in multi-scale levels,and thereby influence the biological functions and comprehensive performance in vitro and in vivo.In particular,a surface promoting the selective adhesion and directional migration of desired types of cells in complex environment is extremely important in the repair and regeneration of tissues such as peripheral nerve and blood vessel,and long-term application of intracorporal devices such as intravascular implants.Therefore,surface modification of biomaterials is a facile and effective method to achieve the desired cell-biomaterials interactions.In this short review,recent advances on the surface modification of biomaterials to regulate selective cell adhesion and migration are briefly summarized.In particular,the surface properties of biomaterials are manipulated via the convenient introduction of amino groups to the ester-based polymers,the formation of polyelectrolyte multilayers,and the fabrication of topology and gradient cues,etc.,followed by the association of chemical and biological signals such as collagen,heparin,hyaluronic acid,peptides and cell growth factors.The selective adhesion and directional migration of various types of cells such as endothelial cells(ECs),smooth muscle cells(SMCs),hepatocytes and Schwann cells(SCs)are achieved over the competitive counterpart cells by the use of cell-resisting substances and cell-selective motifs on gradient substrates in most cases.Recent advances on cell behaviors in three-dimensional(3D)ceH-extracellular matrix(ECM)-mimicking substrates are also reviewed.展开更多
Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from t...Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.展开更多
Objective: To investigate the effect of Malytea Scurfpea fruit (MSF) on adhesion and migration of melanocyte. Methods: Human epidermal melanocytes were treated with MSF and examined for its adhesion to bovine serum fi...Objective: To investigate the effect of Malytea Scurfpea fruit (MSF) on adhesion and migration of melanocyte. Methods: Human epidermal melanocytes were treated with MSF and examined for its adhesion to bovine serum fibronectin-coated culture plate and for its migration into micropore filters, which were then compared with those of untreated control. Results: Compared with the control, MSF treated melanocytes were easier to adhere to the dishes and move into the filters in a dose-dependent manner, but when the dose of MSF was higher than 150 μg/ml, the adhesion and migration could not be significantly increased. And it was noticed that when the concentrations of MSF was 10μg/ml, significant statistical difference did show between the migration of the treated and untreated melanocytes ( P<0.05) though the adhesion was not increased significantly. Conclusion: MSF had the ability to affect melanocyte adhesion and migration. This could explain, in part, the mechanism of MSF in curing vitiligo lesions.展开更多
Objective： To evaluate the effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmo- nary adenocarcinoma A549 cells. Methods： Wound-healing and Transwell invasion assays were performed to ...Objective： To evaluate the effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmo- nary adenocarcinoma A549 cells. Methods： Wound-healing and Transwell invasion assays were performed to study the effect of hypoxia on migration and invasion of A549 cells, and A549 cells were added to a monolayer of human umbilical vein endo-thelial cells （HUVECs） to test the ability to adhere to endothelium. Immunofluorescence assay and luciferase reporter gene assay were also used to evaluate the effect of hypoxia on distribution of E-cadherin, β-catenin, and actin, and hypoxia-inducible factor-1 （HIF-1）-dependent transcription, respectively. Results： Hypoxia facilitated A549 cell migration, invasion, and A549 cell-endothelial cells adhesion, and modulated the distribution of E-cadherin and β-catenin, and actin cytoskeleton rearrange-ment, and up-regulated HIF-l-dependent reporter gene expression in A549 cells. Conclusion： Promotion ofA549 cell migration, invasion, and adhesion on endothelium by hypoxia might be modulated through its up-regulating HIF-l-dependent gene expression, which then induced the redistribution of E-cadherin and β-catenin, and the actin cytoskeletal reorganization.展开更多
Cell-matrix interaction is the key for understanding the cell behaviors,especially the mechanosensitivity of cell adhesion,cell migration and differentiation,etc.Cells are constantly probing,pushing and pulling on the...Cell-matrix interaction is the key for understanding the cell behaviors,especially the mechanosensitivity of cell adhesion,cell migration and differentiation,etc.Cells are constantly probing,pushing and pulling on the surrounding extracellular matrix.These cell-generated forces drive cell migration and tissue morphogenesis,and maintain the intrinsic mechanical tone of tissues.Therefore,knowledge of the spatial and temporal nature of these forces are essential to understanding when and where mechanical events come to play in both physiological and pathological settings.However,because of the complexity of cell geometry and insufficient展开更多
Heterozygous loss-of-function variants of FOXP4 are associated with neurodevelopmental disorders(NDDs)that exhibit delayed speech development,intellectual disability,and congenital abnormalities.The etiology of NDDs i...Heterozygous loss-of-function variants of FOXP4 are associated with neurodevelopmental disorders(NDDs)that exhibit delayed speech development,intellectual disability,and congenital abnormalities.The etiology of NDDs is unclear.Here we found that FOXP4 and N-cadherin are expressed in the nuclei and apical end-feet of radial glial cells(RGCs),respectively,in the mouse neocortex during early gestation.Knockdown or dominant-negative inhibition of Foxp4 abolishes the apical condensation of N-cadherin in RGCs and the integrity of neuroepithelium in the ventricular zone(VZ).Inhibition of Foxp4 leads to impeded radial migration of cortical neurons and ectopic neurogenesis from the proliferating VZ.The ectopic differentiation and deficient migration disappear when N-cadherin is over-expressed in RGCs.The data indicate that Foxp4 is essential for N-cadherin-based adherens junctions,the loss of which leads to periventricular heterotopias.We hypothesize that FOXP4 variant-associated NDDs may be caused by disruption of the adherens junctions and malformation of the cerebral cortex.展开更多
Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital ...Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital of Enshi Autonomous Prefecture between May 2014 and February 2017 were selected as the NB group of the study, and the lesion tissue was collected;38 children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to serious hydronephrosis during the same period were selected as the control group of the study, and the normal adrenal gland tissue was collected. The mRNA expression and protein expression of KLF4 and UBE2C as well as the protein expression of cell adhesion molecules and migration molecules in clinical tissue samples were determined. Results: The mRNA expression and protein expression of KLF4 in neuroblastoma tissue of NB group were greatly lower than those of control group whereas the mRNA expression and protein expression of UBE2C were greatly higher than those of control group;PDLIM1, AMF, GPx1, L1CAM, Nrg1, RANK, RANKL, Inβ1, MTA1 and MMP9 protein expression in neuroblastoma tissue of NB group were greatly higher than those of control group, negatively correlated with the protein expression KLF4, and positively correlated with the protein expression of UBE2C. Conclusion: The low expression of KLF4 and the high expression of UBE2C in neuroblastoma can promote the adhesion and migration of tumor cells.展开更多
Objective:Endometrial tuberculosis,which commonly affects women of reproductive age,is a significant cause of intrauterine adhesions(IUA),potentially leading to hypomenorrhea,amenorrhea,and infertility.Hysteroscopic a...Objective:Endometrial tuberculosis,which commonly affects women of reproductive age,is a significant cause of intrauterine adhesions(IUA),potentially leading to hypomenorrhea,amenorrhea,and infertility.Hysteroscopic adhesiolysis is the primary treatment for IUA;however,studies specifically addressing its efficacy in tuberculosisinduced IUA remain scarce.This study aims to evaluate the therapeutic outcomes of hysteroscopic adhesiolysis for IUA caused by endometrial tuberculosis.Methods:This retrospective cohort study included patients diagnosed with tuberculosisinduced IUA who underwent hysteroscopic adhesiolysis at the Third Xiangya Hospital of Central South University between May 2014 and October 2022.Clinical data including age,medical history,adhesion severity,surgical treatment,and reproductive outcomes were analyzed.Results:Among 39 patients identified,2 were lost to follow-up.A total of 37 patients were included,with a follow-up duration ranging from 6 months to 9 years.Hypomenorrhea was reported in 24(64.9%)patients,secondary amenorrhea in 10(27.0%)patients,and normal menstruation in 3(8.1%)patients.Most patients presented with primary infertility(59.5%),and only 2(5.4%)had secondary infertility.The median American Fertility Society(AFS)score at initial assessment was 10(range,8−12);8(21.6%)patients had moderate IUA,and 29(78.4%)had severe IUA.A total of 86 surgical procedures were performed across 37 patients,with 27 patients undergoing 2 or more surgeries.Postoperatively,25(67.6%)patients achieved normalization of the uterine cavity,while 12(32.4%)still had a reduced cavity.Only 7(18.9%)patients had a grossly normal endometrium at the final surgery,all of whom had moderate adhesions at the initial procedure.Menstrual flow returned to normal in 12(32.4%)patients,while 25(67.6%)continued to experience hypomenorrhea.Of 29 patients who attempted in vitro fertilization and embryo transfer(IVF-ET),only 6(20.7%)conceived.Among these,4(13.8%)delivered at term via cesarean section;one case was complicated by postpartum hemorrhage due to uterine atony and another by placental adhesion.Conclusion:Endometrial tuberculosis can lead to severe IUA.Hysteroscopic adhesiolysis facilitates cavity restoration and improvement of menstrual conditions,but the overall reproductive outcomes remain suboptimal.展开更多
The electrochemical migration (ECM) behavior of the electrically conductive adhesives (ECAs) filled with pure Ag powder or Ag-plated Cu composite powder with varied Ag:Cu ratios was investigated under the condition of...The electrochemical migration (ECM) behavior of the electrically conductive adhesives (ECAs) filled with pure Ag powder or Ag-plated Cu composite powder with varied Ag:Cu ratios was investigated under the condition of applying constant voltage and distilled water environment.ECM resistance was determined from the current-time curves.The microstructure and composition of ECM dendrite products were analyzed by SEM/EDS and XRD.It was found that the ECM resistance of Ag-plated Cu composite powder-filled ECAs was evidently higher than that of pure Ag powder-filled ECAs.The Ag:Cu ratio of composite powder in ECAs had notable influence on ECM resistance,which was enhanced with the decrease of Ag:Cu ratios.The composition of dendrites formed between cathode and anode during ECM process was not uniform for Ag-plated Cu-filled ECAs.An ECM inhibiting mechanism of Ag-plated Cu composite powder was proposed according to analysis of the electrochemical impedance spectroscopy,Tafel plot and dendrite composition.展开更多
基金supported by the National Natural Science Foundation of China(No.52475580)the Special Foundation of the Taishan Scholar Project(No.tsqn202211077)+3 种基金the Shandong Provincial Natural Science Foundation(No.ZR2023ME118)the Open Project of State Key Laboratory of Chemical Safety(No.SKLCS-2024020)the Fundamental Research Funds for the Central Universities(No.24CX02014A)the Fund of State Key Laboratory of Deep Oil and Gas,China University of Petroleum(East China).
文摘In the context of the rapid development of artificial intelligence and robotics,their application scenarios are continuously expanding to a variety of complex environments,with increasing attention being paid to the use of flexible sensors in lowtemperature environments.In this study,an ionic hydrogel was synthesized using acrylamide(AM),hydroxyethyl cellulose(HEC),and lithium chloride(LiCl)as composites.This hydrogel exhibits high adhesion,excellent sensitivity(gauge factor(GF)=2.84),rapid response time(100 ms),exceptional stretch ability(>1776%),high toughness(2.5 MJ/m^(3)),and the ability to maintain detectability at low temperatures(-60℃).HEC imparts reliable mechanical properties to the sensor through hydrogen bonding interactions of its hydroxyl groups.LiCl ensures that the sensor has outstanding antifreezing properties,maintains good conductivity and mechanical performance.Used for robotic attitude detection,the sensor demonstrated accurate recognition of various joint movements at both 20 and -20℃.This technology was extended to industrial operations and maintenance,where a mechanical claw was used to grasp parts at both room temperature and low temperature.A convolutional neural network deep learning algorithm was employed to identify and classify eight types of parts,achieving an impressive recognition accuracy of 98.8%.The polyacrylamide(PAM)/HEC/LiCl hydrogel sensor demonstrates the capability for wide-temperature range detection in flexible robotics,holding significant potential for future applications in human-machine interaction,tactile perception,and related fields.
文摘The published article titled“MicroRNA-133b Inhibits Proliferation,Cellular Migration,and Invasion via Targeting LASP1 in Hepatocarcinoma Cells”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1269–1282.
基金supported by NIH grants,Nos.R01NS125074,R01AG083164,R01NS107365,and R21NS127177(to YL),1F31NS129204-01A1(to KW)and Albert Ryan Fellowship(to KW).
文摘Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postnatal neurogenesis remains unclear.In this study,to define the precise role of transforming growth factor-βsignaling in postnatal neurogenesis at distinct stages of the neurogenic cascade both in vitro and in vivo,we developed two novel inducible and cell type-specific mouse models to specifically silence transforming growth factor-βsignaling in neural stem cells in(mGFAPcre-ALK5fl/fl-Ai9)or immature neuroblasts in(DCXcreERT2-ALK5fl/fl-Ai9).Our data showed that exogenous transforming growth factor-βtreatment led to inhibition of the proliferation of primary neural stem cells while stimulating their migration.These effects were abolished in activin-like kinase 5(ALK5)knockout primary neural stem cells.Consistent with this,inhibition of transforming growth factor-βsignaling with SB-431542 in wild-type neural stem cells stimulated proliferation while inhibited the migration of neural stem cells.Interestingly,deletion of transforming growth factor-βreceptor in neural stem cells in vivo inhibited the migration of postnatal born neurons in mGFAPcre-ALK5fl/fl-Ai9 mice,while abolishment of transforming growth factor-βsignaling in immature neuroblasts in DCXcreERT2-ALK5fl/fl-Ai9 mice did not affect the migration of these cells in the hippocampus.In summary,our data supports a dual role of transforming growth factor-βsignaling in the proliferation and migration of neural stem cells in vitro.Moreover,our data provides novel insights on cell type-specific-dependent requirements of transforming growth factor-βsignaling on neural stem cell proliferation and migration in vivo.
基金supported by the National Natural Science Foundation of China(42430303)Strategy Priority Research Program(Category B)of the Chinese Academy of Sciences(XDB0710000)+2 种基金National Natural Science Foundation of China(42288201)the National Key R&D Program of China(2023YFF0803203)the IGGCAS start-up funding(Grant No.E251510101).
文摘In wave-equation migration and demigration,the cross-correlation imaging/forwarding step implicitly injects an additional copy of the source wavelet,so that the amplitude spectrum of the wavelet is applied redundantly(effectively imposing a wavelet-spectrum weighting,often akin to an amplitude-squared bias).This redundancy degrades structural fidelity and amplitude balance yet is frequently overlooked.We(i)formalize the mechanism by which cross-correlation duplicates the source-wavelet amplitude effect in both migration and demigration,and(ii)introduce a source-equalized operator that removes the redundancy by deconvolving(or dividing by)the wavelet amplitude spectrum in the imaging condition and its demigration counterpart,while leaving phase/kinematics intact.Using a band-limited Ricker wavelet on a two-layer model and on Marmousi,we show that,if unmanaged,the redundant wavelet spectrum broadens main lobes,introduces ringing,and suppresses vertical resolution in migrated images,and inflates spectrum mismatches between demigrated and observed data even when peak times agree.With our correction,images recover observed-data-consistent bandwidth and sharpened interfaces,and demigrated data also exhibit improved spectrum conformity and reduced amplitude misfit.The results clarify when source amplitudes matter,why cross-correlation makes them redundantly matter,and how a lightweight spectral correction restores physically meaningful amplitude behavior in wave-equation migration/demigration.
基金supported by grants from the National Natural Science Foundation of China (No. 30801099)Scientific and Technological Projects in Wuhan City (Nos. 201060938360-05,201051299453)
文摘Summary： In order to study the effects of exogenous sonic hedgehog （shh） peptide on proliferation, adhesion, migration of endothelial progenitor cells （EPCs） from rat peripheral blood, the mononuclear cells were collected from rat peripheral blood by Ficoll density gradient centrifugation. EPCs were iso- lated with adherence screening method and cultured in M199 culture medium with the supplement of VEGF and bFGF. The immunohistochemical staining was used to identify cell markers such as CD133 and VEGFR-2. EPCs were stimulated with exogenous shh peptide of different final concentrations （0.01, 0.1, 1, 10 μg/mL）. The proliferation, adhesion and migration of EPCs were detected by MTT chromom- etry, adhesion test and transwell system, respectively. The results of this study showed that, after 7 days of culture, cells formed clusters, assuming typical cobbles-tone pattern under microscope. After 2 weeks of culture, cells were arranged in cord-like fashion and sometimes grew like ＂micro-vessels＂. Immuno- histochemical staining showed that the cultured cells were positive for both CD133 and VEGFR-2. The proliferation, adhesion and migration of EPCs could be promoted by endogenous shh peptide at concen- trations from 0.1 μg/mL to 10 μg/mL in a concentration-dependent manner. The findings indicate that exogenous shh peptide can enhance EPCs proliferation, adhesion, and migration, which may have a po- tential value for clinical application.
基金supported by The National Science Fund for Distinguished Young Scholars(No.:0602150028)
文摘Objective: To discuss the ef ect of BRMS1 on the proliferation, migration and adhesion of mouse forestomach carcinoma(MFC). Methods: The constructed p CMV-myc-BRMS1 recombinant plasmid and blank plasmid were transfected into mouse forestomach carcinoma. MTT method was employed to measure the activity of gastric cancer cell; the scratch assay and Transwell assay to measure the migration and invasion of gastric cancer cell; the adhesion assay to measure the adhesion of gastric cancer cell; while the Western blot assay to measure the expression of The NF-毷B signal pathway, downstream matrix metalloproteinase(MMP-2), MMP-9 and osteopontin and E-cadherin in the gastric cancer cell. Besides, the transplanted animal model of gastric cancer in mice was constructed to measure the size of tumor xenograft. Results: Results of MTT assay showed that, compared with the empty vector control group, the activity of gastric cancer cell was not af ected in the BRMS1 transfection group. The improved expression of BRMS1 could inhibit the adhesion, migration and invasion of gastric cancer cell(P<0.01). Besides, compared with the empty vector control group, the phosphorylation of NF-毷B p65 and I毷Bα was reduced in the BRMS1 transfection group, with the decreased expression of MMP 2, MMP 9 and osteopontin and the increased expression of E-cadherin(P<0.01). Results of animal experiment also showed that the expression of BRMS1 did not af ect the transplanted tumor. Conclusions: The expression of BRMS1 can signii cantly inhibit the adhesion, migration, invasion and metastasis of MCF gastric cancer cell, which is related to The NF-毷B signal pathway.
基金supported by grants from the Major State Basic Research Development Program of China (973 Program,No. 2009CB521808)the National Natural Sciences Foundation of China (No. 30700895)
文摘It has been reported that metastasis-associated gene 1 （Mta1） is overexpressed in many malignant tumors with high metastatic potential. In addition, some studies indicated that MTA1 participated in invasion, metastasis, and survival of cancer cells by regulating cell migration, adhesion and proliferation. But the role of MTA1 is unclear in vitro in the development of cervical cancer cells. This study investigated whether and how MTA1 mediated cell proliferation, migration, invasion and adhesion in cervical cancer. MTA1 expression level was detected by Western blot in two cervical cancer cell lines of different invasion potentials. The effects of MTA1 expression on SiHa cell apoptosis, cycle, proliferation, migration, invasion and adhesion were tested by flow cytometry, MTT, wound-healing assay, Transwell assay and adhesion assay, respectively. The expression levels of p53, E-cadherin, and β-catenin activity were evaluated in untreated and treated cells. The results showed that MTA1 protein expression was significantly higher in SiHa than in HeLa, which was correlated well with the potential of migration and invasion in both cell lines. Furthermore, the cell invasion, migration and adhesion capabilities were decreased after inhibition of MTA1 expression mediated by Mta1-siRNA transfection in SiHa. However, no significant differences were found in cell apoptosis, cycle, and proliferation. In addition, E-cadherin and p53 protein levels were significantly up-regulated, while β-catenin was significantly down-regulated in SiHa transfected with the siRNA. These results demonstrated that MTA1 played an important role in the migration and invasion of cervical cancer cells. It was speculated that the decreased migration and invasion capability by inhibiting the MTA1 expression in the SiHa cell line may be mediated through the altered expression of p53, and E-cadherin/β-catenin complex. MTA1 could serve as a potential therapeutic target in cervical cancer.
基金supported by the National Natural Science Foundation of China,No.81571211(to FL)the Natural Science Foundation of Shanghai,No.22ZR1476800(to CH)。
文摘Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.
基金Supported by The Fund of Nanjing Medical University Science and Technology Development,No.09NJMUZ30
文摘AIM： To investigate the relationship between Interleu- kin-8 （IL-8） and proliferation, adhesion, migration, inva- sion and chemosensitivity of gastric cancer （GC） cells.
基金supported by grants from National Nature Science Foundation of China(No.30000083)Science and Technology Bureau of Shanghai Municipal Govemment(No.00JC 14042).
文摘Transforming growth factor-b 1 (TGF-β1), a multi-function polypeptide, is a double-edged sword in cancer. For some tumor cells, TGF-β1 is a potent growth inhibitor and apoptosis inducer. More commonly, TGF-β1 loses its growth-inhibitory and apoptosis-inducing effects, but stimulates the metastatic capacity of tumor cells. It is currently little known about TGF-β1-promoted cell migration in hepatocellular carcinoma (HCC) cells, let alone its mechanism. In this study, we found that TGF-β1 lost its tumor-suppressive effects, but significantly stimulated cell migration in SMMC-7721 human HCC cells. By FACS and Western blot analysis, we observed that TGF-β1 enhanced the expression of α5β1 integrin obviously, and subsequently stimulated cell adhesion onto fibronectin (Fn). Furthermore, we observed that TGF-β1 could also promote SMMC-7721 cells adhesion onto laminin (Ln). Our data also provided evidences that TGF-β1 induced epithelial-to-mesenchymal transformation (EMT) in SMMC-7721 cells. First, SMMC-7721 cells clearly switched to the spindle shape morphology after TGF-β1 treatment. Furthermore, TGF-β1 induced the down-regulation of E-cadherin and the nuclear translocation of β-catenin. These results indicated that TGF-β1-promoted cell adhesion and TGF-β1-induced epithelial-to-mesenchymal transformation might be both responsible for TGF-β1-enhanced cell migration.
基金supported by the National Natural Science Foundation of China(11221202and11025208)the State Key Laboratory of Explosive Science and Technology of Beijing Institute of Technology(YBKT12-05)
文摘Cell adhesion and migration are basic physiolog- ical processes in living organisms. Cells can actively probe their mechanical micro-environment and respond to the ex- ternal stimuli through cell adhesion. Cells need to move to the targeting place to perform function via cell migration. For adherent cells, cell migration is mediated by cell-matrix adhesion and cell-cell adhesion. Experimental approaches, especially at early stage of investigation, are indispensable to studies of cell mechanics when even qualitative behaviors of cell as well as fundamental factors in cell behaviors are unclear. Currently, there is increasingly accumulation of ex- perimental data of measurement, thus a quantitative formula- tion of cell behaviors and the relationship among these fun- damental factors are highly needed. This quantitative under- standing should be crucial to tissue engineering and biomed- ical engineering when people want to accurately regulate or control cell behaviors from single cell level to tissue level. In this review, we will elaborate recent advances in the ex- perimental and theoretical studies on cell adhesion and mi- gration, with particular focuses laid on recent advances in experimental techniques and theoretical modeling, through which challenging problems in the cell mechanics are sug- gested.
基金the National Key Research and Development Program of China(No.2016YFC1100403)the National Natural Science Foundation of China(No.21434006)the Fundamental Research Funds for the Central Universities of China(No.2020XZZX004-01).
文摘The surfaces and interfaces of biomaterials interact with the biological systems in multi-scale levels,and thereby influence the biological functions and comprehensive performance in vitro and in vivo.In particular,a surface promoting the selective adhesion and directional migration of desired types of cells in complex environment is extremely important in the repair and regeneration of tissues such as peripheral nerve and blood vessel,and long-term application of intracorporal devices such as intravascular implants.Therefore,surface modification of biomaterials is a facile and effective method to achieve the desired cell-biomaterials interactions.In this short review,recent advances on the surface modification of biomaterials to regulate selective cell adhesion and migration are briefly summarized.In particular,the surface properties of biomaterials are manipulated via the convenient introduction of amino groups to the ester-based polymers,the formation of polyelectrolyte multilayers,and the fabrication of topology and gradient cues,etc.,followed by the association of chemical and biological signals such as collagen,heparin,hyaluronic acid,peptides and cell growth factors.The selective adhesion and directional migration of various types of cells such as endothelial cells(ECs),smooth muscle cells(SMCs),hepatocytes and Schwann cells(SCs)are achieved over the competitive counterpart cells by the use of cell-resisting substances and cell-selective motifs on gradient substrates in most cases.Recent advances on cell behaviors in three-dimensional(3D)ceH-extracellular matrix(ECM)-mimicking substrates are also reviewed.
文摘Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.
基金This study was supported by National Natural Sciences Foundation of China (No. 30200247)
文摘Objective: To investigate the effect of Malytea Scurfpea fruit (MSF) on adhesion and migration of melanocyte. Methods: Human epidermal melanocytes were treated with MSF and examined for its adhesion to bovine serum fibronectin-coated culture plate and for its migration into micropore filters, which were then compared with those of untreated control. Results: Compared with the control, MSF treated melanocytes were easier to adhere to the dishes and move into the filters in a dose-dependent manner, but when the dose of MSF was higher than 150 μg/ml, the adhesion and migration could not be significantly increased. And it was noticed that when the concentrations of MSF was 10μg/ml, significant statistical difference did show between the migration of the treated and untreated melanocytes ( P<0.05) though the adhesion was not increased significantly. Conclusion: MSF had the ability to affect melanocyte adhesion and migration. This could explain, in part, the mechanism of MSF in curing vitiligo lesions.
文摘Objective： To evaluate the effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmo- nary adenocarcinoma A549 cells. Methods： Wound-healing and Transwell invasion assays were performed to study the effect of hypoxia on migration and invasion of A549 cells, and A549 cells were added to a monolayer of human umbilical vein endo-thelial cells （HUVECs） to test the ability to adhere to endothelium. Immunofluorescence assay and luciferase reporter gene assay were also used to evaluate the effect of hypoxia on distribution of E-cadherin, β-catenin, and actin, and hypoxia-inducible factor-1 （HIF-1）-dependent transcription, respectively. Results： Hypoxia facilitated A549 cell migration, invasion, and A549 cell-endothelial cells adhesion, and modulated the distribution of E-cadherin and β-catenin, and actin cytoskeleton rearrange-ment, and up-regulated HIF-l-dependent reporter gene expression in A549 cells. Conclusion： Promotion ofA549 cell migration, invasion, and adhesion on endothelium by hypoxia might be modulated through its up-regulating HIF-l-dependent gene expression, which then induced the redistribution of E-cadherin and β-catenin, and the actin cytoskeletal reorganization.
基金supported by the National Natural Science Foundation of China through grant nos.11221202 and 11025208
文摘Cell-matrix interaction is the key for understanding the cell behaviors,especially the mechanosensitivity of cell adhesion,cell migration and differentiation,etc.Cells are constantly probing,pushing and pulling on the surrounding extracellular matrix.These cell-generated forces drive cell migration and tissue morphogenesis,and maintain the intrinsic mechanical tone of tissues.Therefore,knowledge of the spatial and temporal nature of these forces are essential to understanding when and where mechanical events come to play in both physiological and pathological settings.However,because of the complexity of cell geometry and insufficient
基金supported by the Wenzhou Municipal Science and Technology Bureau(Y20210901)the Natural Science Foundation of Zhejiang Province(LQ20H090001)the Scientific Research Fund of Wenling Science and Technology Bureau(2018C320001).
文摘Heterozygous loss-of-function variants of FOXP4 are associated with neurodevelopmental disorders(NDDs)that exhibit delayed speech development,intellectual disability,and congenital abnormalities.The etiology of NDDs is unclear.Here we found that FOXP4 and N-cadherin are expressed in the nuclei and apical end-feet of radial glial cells(RGCs),respectively,in the mouse neocortex during early gestation.Knockdown or dominant-negative inhibition of Foxp4 abolishes the apical condensation of N-cadherin in RGCs and the integrity of neuroepithelium in the ventricular zone(VZ).Inhibition of Foxp4 leads to impeded radial migration of cortical neurons and ectopic neurogenesis from the proliferating VZ.The ectopic differentiation and deficient migration disappear when N-cadherin is over-expressed in RGCs.The data indicate that Foxp4 is essential for N-cadherin-based adherens junctions,the loss of which leads to periventricular heterotopias.We hypothesize that FOXP4 variant-associated NDDs may be caused by disruption of the adherens junctions and malformation of the cerebral cortex.
文摘Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital of Enshi Autonomous Prefecture between May 2014 and February 2017 were selected as the NB group of the study, and the lesion tissue was collected;38 children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to serious hydronephrosis during the same period were selected as the control group of the study, and the normal adrenal gland tissue was collected. The mRNA expression and protein expression of KLF4 and UBE2C as well as the protein expression of cell adhesion molecules and migration molecules in clinical tissue samples were determined. Results: The mRNA expression and protein expression of KLF4 in neuroblastoma tissue of NB group were greatly lower than those of control group whereas the mRNA expression and protein expression of UBE2C were greatly higher than those of control group;PDLIM1, AMF, GPx1, L1CAM, Nrg1, RANK, RANKL, Inβ1, MTA1 and MMP9 protein expression in neuroblastoma tissue of NB group were greatly higher than those of control group, negatively correlated with the protein expression KLF4, and positively correlated with the protein expression of UBE2C. Conclusion: The low expression of KLF4 and the high expression of UBE2C in neuroblastoma can promote the adhesion and migration of tumor cells.
基金supported by the Wisdom Accumulation and Talent Cultivation Project of Third Xiangya Hosipital of Central South University,China(YX202112).
文摘Objective:Endometrial tuberculosis,which commonly affects women of reproductive age,is a significant cause of intrauterine adhesions(IUA),potentially leading to hypomenorrhea,amenorrhea,and infertility.Hysteroscopic adhesiolysis is the primary treatment for IUA;however,studies specifically addressing its efficacy in tuberculosisinduced IUA remain scarce.This study aims to evaluate the therapeutic outcomes of hysteroscopic adhesiolysis for IUA caused by endometrial tuberculosis.Methods:This retrospective cohort study included patients diagnosed with tuberculosisinduced IUA who underwent hysteroscopic adhesiolysis at the Third Xiangya Hospital of Central South University between May 2014 and October 2022.Clinical data including age,medical history,adhesion severity,surgical treatment,and reproductive outcomes were analyzed.Results:Among 39 patients identified,2 were lost to follow-up.A total of 37 patients were included,with a follow-up duration ranging from 6 months to 9 years.Hypomenorrhea was reported in 24(64.9%)patients,secondary amenorrhea in 10(27.0%)patients,and normal menstruation in 3(8.1%)patients.Most patients presented with primary infertility(59.5%),and only 2(5.4%)had secondary infertility.The median American Fertility Society(AFS)score at initial assessment was 10(range,8−12);8(21.6%)patients had moderate IUA,and 29(78.4%)had severe IUA.A total of 86 surgical procedures were performed across 37 patients,with 27 patients undergoing 2 or more surgeries.Postoperatively,25(67.6%)patients achieved normalization of the uterine cavity,while 12(32.4%)still had a reduced cavity.Only 7(18.9%)patients had a grossly normal endometrium at the final surgery,all of whom had moderate adhesions at the initial procedure.Menstrual flow returned to normal in 12(32.4%)patients,while 25(67.6%)continued to experience hypomenorrhea.Of 29 patients who attempted in vitro fertilization and embryo transfer(IVF-ET),only 6(20.7%)conceived.Among these,4(13.8%)delivered at term via cesarean section;one case was complicated by postpartum hemorrhage due to uterine atony and another by placental adhesion.Conclusion:Endometrial tuberculosis can lead to severe IUA.Hysteroscopic adhesiolysis facilitates cavity restoration and improvement of menstrual conditions,but the overall reproductive outcomes remain suboptimal.
基金financially supported by the Special Funds of National Ministry of Finance for the Transformation of Industrial Technology Achievements (No.CJ2007-475)
文摘The electrochemical migration (ECM) behavior of the electrically conductive adhesives (ECAs) filled with pure Ag powder or Ag-plated Cu composite powder with varied Ag:Cu ratios was investigated under the condition of applying constant voltage and distilled water environment.ECM resistance was determined from the current-time curves.The microstructure and composition of ECM dendrite products were analyzed by SEM/EDS and XRD.It was found that the ECM resistance of Ag-plated Cu composite powder-filled ECAs was evidently higher than that of pure Ag powder-filled ECAs.The Ag:Cu ratio of composite powder in ECAs had notable influence on ECM resistance,which was enhanced with the decrease of Ag:Cu ratios.The composition of dendrites formed between cathode and anode during ECM process was not uniform for Ag-plated Cu-filled ECAs.An ECM inhibiting mechanism of Ag-plated Cu composite powder was proposed according to analysis of the electrochemical impedance spectroscopy,Tafel plot and dendrite composition.
