BACKGROUND:The potential effect of graft steatosis on the postoperative liver function is discussed controversially. The present study aimed to evaluate the effect of the donor liver microvesicular steatosis on the po...BACKGROUND:The potential effect of graft steatosis on the postoperative liver function is discussed controversially. The present study aimed to evaluate the effect of the donor liver microvesicular steatosis on the postoperative outcome after liver transplantation.METHODS:Ninety-four patients undergoing liver transplantation at the University Hospital Aachen were included in this study. The patient cohort was divided into three groups according to the grade of microvesicular steatosis(MiS):MiS <30%(n=27), MiS 30%-60%(n=41) and MiS >60%(n=26).The outcomes after liver transplantation were evaluated, including the 30-day and 1-year patient and graft survival rates and the incidences of early allograft dysfunction(EAD) and primary nonfunction(PNF). RESULTS:The incidences of EAD and PNF did not differ significantly between the groups. We observed 5 cases of PNF,one occurred in the MiS <30% group and 4 in the MiS 30%-60% group. The 30-day and 1-year graft survivals did not differ significantly between groups. The 30-day patient survival rates were 100% in all groups. The 1-year patient survival rates were 94.4% in the MiS <30% group, 87.9% in the MiS 30%-60% group and 90.9% in the MiS >60% group.CONCLUSION:Microvesicular steatosis of donor livers has no negative effect on the postoperative outcome after liver transplantation.展开更多
Background:Perfluorooctanoic acid(PFOA)is an environmental contaminant associated with adverse metabolic outcomes in developmentally exposed human populations and mouse models.Hexafluoropropylene oxide-dimer acid(HFPO...Background:Perfluorooctanoic acid(PFOA)is an environmental contaminant associated with adverse metabolic outcomes in developmentally exposed human populations and mouse models.Hexafluoropropylene oxide-dimer acid(HFPO-DA,commonly called GenX)has replaced PFOA in many industrial applications in the U.S.and Europe and has been measured in global water systems from<1 to 9350 ng/L HFPO-DA.Health effects data for GenX are lacking.Objective:Determine the effects of gestational exposure to GenX on offspring weight gain trajectory,adult metabolic health,liver pathology and key adipose gene pathways in male and female CD-1 mice.Methods:Daily oral doses of GenX(0.2,1.0,2.0 mg/kg),PFOA(0.1,1.0 mg/kg),or vehicle control were administered to pregnant mice(gestation days 1.5-17.5).Offspring were fed a high-or low-fat diet(HFD or LFD)at weaning until necropsy at 6 or 18 weeks,and metabolic endpoints were measured over time.PFOA and GenX serum and urine concentrations,weight gain,serum lipid parameters,body mass composition,glucose tolerance,white adipose tissue gene expression,and liver histopathology were evaluated.Results:Prenatal exposure to GenX led to its accumulation in the serum and urine of 5-day old pups(P=0.007,P<0.001),which was undetectable by weaning.By 18 weeks of age,male mice fed LFD in the 2.0 mg/kg GenX group displayed increased weight gain(P<0.05),fat mass(P=0.016),hepatocellular microvesicular fatty change(P=0.015),and insulin sensitivity(P=0.014)in comparison to control males fed LFD.Female mice fed HFD had a significant increase in hepatocyte single cell necrosis in 1.0 mg/kg GenX group(P=0.022)and 1.0 mg/kg PFOA group(P=0.003)compared to control HFD females.Both sexes were affected by gestational GenX exposure;however,the observed phenotype varied between sex with males displaying more characteristics of metabolic disease and females exhibiting liver damage in response to the gestational exposure.Conclusions:Prenatal exposure to 1 mg/kg GenX and 1 mg/kg PFOA induces adverse metabolic outcomes in adult mice that are diet-and sex-dependent.GenX also accumulated in pup serum,suggesting that placental and potentially lactational transfer are important exposure routes for GenX.展开更多
文摘BACKGROUND:The potential effect of graft steatosis on the postoperative liver function is discussed controversially. The present study aimed to evaluate the effect of the donor liver microvesicular steatosis on the postoperative outcome after liver transplantation.METHODS:Ninety-four patients undergoing liver transplantation at the University Hospital Aachen were included in this study. The patient cohort was divided into three groups according to the grade of microvesicular steatosis(MiS):MiS <30%(n=27), MiS 30%-60%(n=41) and MiS >60%(n=26).The outcomes after liver transplantation were evaluated, including the 30-day and 1-year patient and graft survival rates and the incidences of early allograft dysfunction(EAD) and primary nonfunction(PNF). RESULTS:The incidences of EAD and PNF did not differ significantly between the groups. We observed 5 cases of PNF,one occurred in the MiS <30% group and 4 in the MiS 30%-60% group. The 30-day and 1-year graft survivals did not differ significantly between groups. The 30-day patient survival rates were 100% in all groups. The 1-year patient survival rates were 94.4% in the MiS <30% group, 87.9% in the MiS 30%-60% group and 90.9% in the MiS >60% group.CONCLUSION:Microvesicular steatosis of donor livers has no negative effect on the postoperative outcome after liver transplantation.
文摘Background:Perfluorooctanoic acid(PFOA)is an environmental contaminant associated with adverse metabolic outcomes in developmentally exposed human populations and mouse models.Hexafluoropropylene oxide-dimer acid(HFPO-DA,commonly called GenX)has replaced PFOA in many industrial applications in the U.S.and Europe and has been measured in global water systems from<1 to 9350 ng/L HFPO-DA.Health effects data for GenX are lacking.Objective:Determine the effects of gestational exposure to GenX on offspring weight gain trajectory,adult metabolic health,liver pathology and key adipose gene pathways in male and female CD-1 mice.Methods:Daily oral doses of GenX(0.2,1.0,2.0 mg/kg),PFOA(0.1,1.0 mg/kg),or vehicle control were administered to pregnant mice(gestation days 1.5-17.5).Offspring were fed a high-or low-fat diet(HFD or LFD)at weaning until necropsy at 6 or 18 weeks,and metabolic endpoints were measured over time.PFOA and GenX serum and urine concentrations,weight gain,serum lipid parameters,body mass composition,glucose tolerance,white adipose tissue gene expression,and liver histopathology were evaluated.Results:Prenatal exposure to GenX led to its accumulation in the serum and urine of 5-day old pups(P=0.007,P<0.001),which was undetectable by weaning.By 18 weeks of age,male mice fed LFD in the 2.0 mg/kg GenX group displayed increased weight gain(P<0.05),fat mass(P=0.016),hepatocellular microvesicular fatty change(P=0.015),and insulin sensitivity(P=0.014)in comparison to control males fed LFD.Female mice fed HFD had a significant increase in hepatocyte single cell necrosis in 1.0 mg/kg GenX group(P=0.022)and 1.0 mg/kg PFOA group(P=0.003)compared to control HFD females.Both sexes were affected by gestational GenX exposure;however,the observed phenotype varied between sex with males displaying more characteristics of metabolic disease and females exhibiting liver damage in response to the gestational exposure.Conclusions:Prenatal exposure to 1 mg/kg GenX and 1 mg/kg PFOA induces adverse metabolic outcomes in adult mice that are diet-and sex-dependent.GenX also accumulated in pup serum,suggesting that placental and potentially lactational transfer are important exposure routes for GenX.
