Histochemical study and determination of cytochrome oxidase (CTO) relative activ-ity with a Leitz MPV-III microspectrophotometer in different regions of normal rat brain werecarried out.9 healthy male Wistar rats were...Histochemical study and determination of cytochrome oxidase (CTO) relative activ-ity with a Leitz MPV-III microspectrophotometer in different regions of normal rat brain werecarried out.9 healthy male Wistar rats were divided randomly into 2 groups;an enzyme activi-ty studied group and a control group with HE staining.It was found that 2 kinds of CTO distri-bution areas exist in the brain of rats;the high activity area including cerebral cortex,corpusstriatum (gray matter),thalamus,cerebellar cortex,etc,and low activity area including corpuscallosum,corpus striatum (white matter),hippocampus,cerebellar white matter,etc.The dif-ference of CTO activity between the 2 areas is statistically significant (P【0.01).Moreover,according to the intensity of positive response to CTO detecting stain,the granular layer of thecerebellum can be classified as cytochrome oxidase richly-contained area(CTORA)orcytochrome oxidase poorly-contained area (CTOPA).The CTO activity of the former issignificantly higher than that of the latter(P【0.01).展开更多
文摘Histochemical study and determination of cytochrome oxidase (CTO) relative activ-ity with a Leitz MPV-III microspectrophotometer in different regions of normal rat brain werecarried out.9 healthy male Wistar rats were divided randomly into 2 groups;an enzyme activi-ty studied group and a control group with HE staining.It was found that 2 kinds of CTO distri-bution areas exist in the brain of rats;the high activity area including cerebral cortex,corpusstriatum (gray matter),thalamus,cerebellar cortex,etc,and low activity area including corpuscallosum,corpus striatum (white matter),hippocampus,cerebellar white matter,etc.The dif-ference of CTO activity between the 2 areas is statistically significant (P【0.01).Moreover,according to the intensity of positive response to CTO detecting stain,the granular layer of thecerebellum can be classified as cytochrome oxidase richly-contained area(CTORA)orcytochrome oxidase poorly-contained area (CTOPA).The CTO activity of the former issignificantly higher than that of the latter(P【0.01).
