Ustiloxins are the primary secondary metabolites of Ustilaginoidea virens(teleomorph:Villosiclava virens).While these toxins are known to exhibit toxicity in certain animals,their potential risks to human health requi...Ustiloxins are the primary secondary metabolites of Ustilaginoidea virens(teleomorph:Villosiclava virens).While these toxins are known to exhibit toxicity in certain animals,their potential risks to human health require further investigation.Therefore,this study was conducted using male mice as experimental animal to explore their potential health risks.We observed morphological changes in cells and structural lesions in mitochondria using electron microscopy,combined with Hematoxylin and Eosin(H&E)staining.We utilized transcriptomics,proteomics,and bioinformatics techniques to analyze the regulatory networks associated with ustiloxins in the context of liver damage and study the mechanism of liver injury caused by ustiloxins in mice.Differentially expressed genes(DEGs)were linked to terms such as‘metabolism of exogenous substances with cytochrome P450’,‘peroxisomes’,and‘negative regulation of the inflammatory response’.Western blotting and quantitative real-time PCR(qRT-PCR)demonstrated that ustiloxins can further activate the inflammatory pathways of Nrf2/HO-1 and NLRP3 by activating cytochrome P450(CYP450),leading to liver damage in mice.展开更多
基金supported by the General Scientific Research Project of the Zhejiang Education Department,China(Grant No.Y202249221).
文摘Ustiloxins are the primary secondary metabolites of Ustilaginoidea virens(teleomorph:Villosiclava virens).While these toxins are known to exhibit toxicity in certain animals,their potential risks to human health require further investigation.Therefore,this study was conducted using male mice as experimental animal to explore their potential health risks.We observed morphological changes in cells and structural lesions in mitochondria using electron microscopy,combined with Hematoxylin and Eosin(H&E)staining.We utilized transcriptomics,proteomics,and bioinformatics techniques to analyze the regulatory networks associated with ustiloxins in the context of liver damage and study the mechanism of liver injury caused by ustiloxins in mice.Differentially expressed genes(DEGs)were linked to terms such as‘metabolism of exogenous substances with cytochrome P450’,‘peroxisomes’,and‘negative regulation of the inflammatory response’.Western blotting and quantitative real-time PCR(qRT-PCR)demonstrated that ustiloxins can further activate the inflammatory pathways of Nrf2/HO-1 and NLRP3 by activating cytochrome P450(CYP450),leading to liver damage in mice.
