Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,inclu...Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,including reducing inflammatory response,improving immunity and enhancing antioxidant levels.In this prospective randomized double-blinded study,we wanted to explore the clinical efficacy and safety of Kangfuxin liquid combined with microneedling in treating facial skin photoaging.Methods:57 patients with facial skin photoaging were randomly divided into two groups.The treatment group(28 cases)received microneedle therapy with Kangfuxin liquid,while the control group(29 cases)received microneedle therapy with physiological saline.The treatment interval was 4 weeks,and a total of 3 treatments were performed.Compare the VISIA scores of facial photoaging features such as wrinkles,texture,pores,spots and ultraviolet pigmentation between two groups of patients before and after treatment,Global Score for Photoaging,satisfaction evaluation,and record the occurrence of adverse reactions.Results:After treatment,the treatment group showed more significant improvement in wrinkles,texture,pores,spots and ultraviolet pigmentation,and the Global Score for Photoaging was better than the control group(P<0.05).The satisfaction rate with improving skin in the treatment group was 85.71%,which was higher than 75.86%in the control group(P<0.05).Both groups did not experience adverse reactions such as skin infection,pigmentation or hypopigmentation,scar formation,or worsening of melasma.Conclusion:Kangfuxin liquid combined with microneedling therapy has a good improvement effect on facial skin photoaging,with a low incidence of adverse reactions and high patient satisfaction.It is worthy of clinical promotion.展开更多
Objective:To combine with transdermal drug delivery using microneedle to simulate the bee venom therapy to evaluate the permeation of bee venom gel.Methods:In this study,the sodium urate and LPS were used on rats and ...Objective:To combine with transdermal drug delivery using microneedle to simulate the bee venom therapy to evaluate the permeation of bee venom gel.Methods:In this study,the sodium urate and LPS were used on rats and mice to construct the model.Bee venom gelemicroneedle combination effect on the model is to determine the role of microneedle gel permeation by observing inflammation factors.Results:Compared with the model group,the bee venom gelemicroneedle combination group can reduce the level of serum nitric oxide of the acute gouty inflammation model caused by sodium urate,and on LPS induced mouse model of acute inflammation effect and the micro.Conclusions:Bee venom can significantly suppress the occurrence of gouty arthritis inflammation in rats and mice LPS inflammatory reaction.Choose the 750 mm microneedle with 10N force on skin about 3 minutes,bee venom can play the optimal role,and the anti-inflammatory effect is obvious.Microneedles can promote the percutaneous absorption of the active macromolecules bee venom gel.展开更多
Objective:Current treatments for atrophic acne scars such as microneedling radiofrequency(MNRF)and fractional carbon dioxide(FCO_(2))laser have shown individual efficacy,but their combined effectiveness remains lackin...Objective:Current treatments for atrophic acne scars such as microneedling radiofrequency(MNRF)and fractional carbon dioxide(FCO_(2))laser have shown individual efficacy,but their combined effectiveness remains lacking.This study aimed to determine the effectiveness and safety of a sequential combination therapy with MNRF followed by FCO_(2) laser compared with each therapy administered alone for the treatment of atrophic acne scars.Methods:An open randomized trial was conducted on 45 patients with atrophic acne scars at Al-Salam Teaching Hospital,Mosul,Iraq,from January 2022 to July 2023.The patients were randomized into 3 groups,with 15 patients in each group:FCO_(2) group(6 sessions of FCO_(2) laser treatment),MNRF group(6 sessions of MNRF treatment),and combined group(3 sessions of MNRF treatment followed by 3 sessions of FCO_(2) laser treatment).The Goodman and Baron Global Quantitative Acne Scar Grading System(GBGQASGS)was used to assess the treatment effectiveness.Adverse effects of treatment were monitored.The pre-treatment and post-treatment GBGQASGS scores and the percentage reductions in the GBGQASGS scores in the 3 groups were assessed by analysis of variance.The effect size of each treatment regimen was assessed using the Cohen d effect size.Results:The 45 patients enrolled comprised 20(44.4%)men and 25(55.6%)women,with a mean age of 25.10±2.80 years and a GBGQASGS score of 28.93±6.19.All 3 types of treatment significantly reduced the GBGQASGS scores after 6 sessions(all P<0.05)as following:FCO_(2) group,from 28.66±5.71 to 11.66±2.71(59.3%reduction;Cohen d=3.80);MNRF group,from 27.26±6.93 to 11.66±3.09(57.2%reduction;Cohen d=2.90);Combined group,from 30.87±5.73 to 9.01±2.03(70.8%reduction;Cohen d=5.08).There was no difference in the GBGQASGS score of post-treatment among 3 groups(P=0.300),however,the combined group exhibited a significant reduction in the GBGQASGS score from pre-treatment to post-treatment(P<0.001).Post-inflammatory hyperpigmentation was reported in FCO_(2) and MNRF groups,tram-track scarring was found in FCO_(2) group,while no serious adverse effects were reported in the combined group.Conclusion:Sequential combination therapy with MNRF followed by FCO_(2) laser was as effective as each monotherapy for the treatment of atrophic acne scars with fewer adverse effects.The results may provide evidence for treatment choice.展开更多
Microneedles(MNs)have been extensively investigated for transdermal delivery of large-sized drugs,including proteins,nucleic acids,and even extracellular vesicles(EVs).However,for their sufficient skin penetration,con...Microneedles(MNs)have been extensively investigated for transdermal delivery of large-sized drugs,including proteins,nucleic acids,and even extracellular vesicles(EVs).However,for their sufficient skin penetration,conventional MNs employ long needles(≥600μm),leading to pain and skin irritation.Moreover,it is critical to stably apply MNs against complex skin surfaces for uniform nanoscale drug delivery.Herein,a dually amplified transdermal patch(MN@EV/SC)is developed as the stem cell-derived EV delivery platform by hierarchically integrating an octopusinspired suction cup(SC)with short MNs(≤300μm).While leveraging the suction effect to induce nanoscale deformation of the stratum corneum,MN@EV/SC minimizes skin damage and enhances the adhesion of MNs,allowing EV to penetrate deeper into the dermis.When MNs of various lengths are applied to mouse skin,the short MNs can elicit comparable corticosterone release to chemical adhesives,whereas long MNs induce a prompt stress response.MN@EV/SC can achieve a remarkable penetration depth(290μm)for EV,compared to that of MN alone(111μm).Consequently,MN@EV/SC facilitates the revitalization of fibroblasts and enhances collagen synthesis in middle-aged mice.Overall,MN@EV/SC exhibits the potential for skin regeneration by modulating the dermal microenvironment and ensuring patient comfort.展开更多
Microneedle technology has undergone a paradigm shift from basic transdermal drug delivery to intelligent,closed-loop theranostic systems.Hydrogel materials have emerged as core carriers due to their excellent biocomp...Microneedle technology has undergone a paradigm shift from basic transdermal drug delivery to intelligent,closed-loop theranostic systems.Hydrogel materials have emerged as core carriers due to their excellent biocompatibility,efficient drug loading capacity,and improved patient compliance.Moreover,critical bottlenecks in hydrogel microneedles,including poor mechanical strength,burst release of drugs,and delayed response to treatment,can be addressed via cross-scale integration of nanomaterials.This review systematically outlines several multiscale engineering strategies to overcome these limitations.The construction of nanotopological networks coupled with dynamic crosslinking modulation synergistically enhances the mechanical properties,stability of drug loading,and conductivity of hydrogel microneedles.Furthermore,responsive nanocarriers equipped with biosensors help establish a closed-loop linkage between monitoring and therapeutic functions.We highlight their synergistic theranostic advantages in scenarios such as wound regulation and tumor-immune microenvironments,while revealing the role in integrating flexible electronics with wearable systems in intelligent medicine.We also summarize the research advances on the biosafety and scalable manufacturing processes of nanocomposite hydrogel m icroneedles(NHMNs),providing examples of clinical translation to elucidate the path from fundamental research to industrial implementation.As a convergence of nanotechnology,biomaterials,and flexible electronics,NHMNs provide new standards for transdermal theranostics as well as a roadmap for iterative advancement of intelligent theranostic devices in personalized medicine.Their cross-scale collaborative design,which spans from the properties of materials to the functional integration of macroscopic devices,can facilitate potential breakthroughs in next-generation closed-loop theranostic systems.展开更多
The clinical management of hypertrophic scars(HSs)remains challenging due to their complex etiology and heterogeneous morphology,underscoring the need for multitarget treatment strategies.In this study,we developed a ...The clinical management of hypertrophic scars(HSs)remains challenging due to their complex etiology and heterogeneous morphology,underscoring the need for multitarget treatment strategies.In this study,we developed a nanocomposite system constructed through the metal-phenolic network-mediated self-assembly of molybdenum polyoxometalate({Mo 154})and epigallocatechin gallate(EGCG),followed by chitosan encapsulation,to generate chitosan-encapsulated{Mo 154}/EGCG(CME)nanoparticles.These nanoparticles were integrated into dissolvable microneedles(CME@MN)to enable transdermal administration.Under near-infrared laser irradiation,CME exhibited a three-pronged therapeutic effect:suppression of collagen overproduction and excessive extracellular matrix(ECM)deposition in human keloid fibroblasts,regulation of proliferation and migration in human umbilical vein endothelial cells,and reprogramming of macrophages toward a proinflammatory M1 phenotype.In vivo,CME@MN patches preferentially accumulated within scar tissue,where they normalized ECM organization,improved collagen fiber rearrangement,and attenuated fibroblast activity through photothermal-enhanced mechanisms while maintaining an excellent safety profile.The CME@MN system represents a potentially transformative approach to HS management by offering a unified platform that simultaneously targets the fibrotic,angiogenic,and inflammatory components of scar pathogenesis.展开更多
Biofabrication and biomanufacturing are rapidly transforming how materials,therapeutics,and functional biological constructs are produced.These fields integrate developments in sustainable biomaterials,precision fabri...Biofabrication and biomanufacturing are rapidly transforming how materials,therapeutics,and functional biological constructs are produced.These fields integrate developments in sustainable biomaterials,precision fabrication,biological systems,and data-driven engineering to produce scalable,efficient,and environmentally aligned production pathways.This review highlights recent scientific advances led by researchers in Singapore,focusing on three interconnected pillars:sustainable bio derived materials,enabling fabrication and manufacturing technologies,and emerging applications.We first examine the expanding use of biomass-derived feedstocks,including human hair keratin,aquaculture side-streams,and plant-derived polysaccharides,which support circular and resource-conscious material development.We then present advances in biofabrication technologies,including electrospinning,three-dimensional bioprinting,and metal additive manufacturing,that enable improved control over the structure,function,and manufacturability of biomedical and functional constructs.Emerging applications,such as machine learning-assisted additive manufacturing,food biomanufacturing,regenerative cell therapy,microneedles,and bioelectronics,exemplify how biofabrication and biomanufacturing are increasingly interrelated across the health,materials,and technological domains.These research contributions from Singapore exemplify how sustainable feedstocks,digital and automated fabrication platforms,and biologically driven applications are shaping the evolving landscape of biofabrication and biomanufacturing.The convergence of materials science,biological engineering,and advanced manufacturing continues to enable new opportunities for innovation in biomedical,industrial,and societal contexts.展开更多
Subretinal injection(SI)is recognized as the most effective method for treating retinal degenerative and genetic diseases,enabling targeted delivery of therapeutic agents such as viral vectors or stem cells directly i...Subretinal injection(SI)is recognized as the most effective method for treating retinal degenerative and genetic diseases,enabling targeted delivery of therapeutic agents such as viral vectors or stem cells directly into the subretinal space(SRS).However,this procedure is typically performed under extremely constrained intraocular conditions,characterized by limited visualization,lack of tactile feedback,and the inherent fragility of retinal tissues,significantly increasing the risk of surgical complications and potential vision impairment.To overcome these challenges,an integrated force-sensing microneedle equipped with a flexible joint was developed and integrated into an ophthalmic surgical robotic system.This advanced system provides precise needle-tip pose control and real-time puncture force feedback.Leveraging these capabilities,force-guided autonomous retinal puncture was achieved,ensuring accurate and consistent drug delivery into the SRS.Comparative experiments conducted on live Bama pigs demonstrated the robotic system's superiority over manual SI techniques,as evidenced by more stable puncture trajectories,smoother insertion velocities,precise insertion depths,and a greater than 90%reduction in average puncture force,despite respiratory and cardiac-induced disturbances,thus significantly enhancing surgical precision and minimizing the risk of iatrogenic retinal injury.Postoperative evaluations further validated that the robotic approach markedly reduced drug reflux into the vitreous cavity,underscoring its reliability and safety for SI.展开更多
Soft robots,characterized by compliance,adaptability,and multimodal responsiveness,represent a rapidly advancing frontier in biomedical applications,wearable technologies,and environmental exploration.This review summ...Soft robots,characterized by compliance,adaptability,and multimodal responsiveness,represent a rapidly advancing frontier in biomedical applications,wearable technologies,and environmental exploration.This review summarizes recent progress in soft robotics with a focus on material innovation,structural design,functional integration,and intelligent responsiveness.Emphasis is placed on the development of bioinspired and stimuli-responsive materials,the construction of modular and reconfigurable architectures,and the integration of actuation,sensing,and energy systems.Microneedle array-based soft robots and hydrogel-based 4D-printed systems are introduced as representative platforms for drug delivery,wound healing,and environmental monitoring.Key challenges,including limited durability,power autonomy,and multifunctional synergy,are critically analyzed in relation to practical operation and long-term reliability.Future directions involve the convergence of self-healing materials,intelligent control algorithms,and multiscale integration strategies to achieve enhanced adaptability and clinical translation.This review provides a comprehensive overview of the interdisciplinary development of next-generation soft robots that bridge materials science,biomedical engineering,and intelligent systems,paving the way toward real-world applications.展开更多
Aim:The aim of this literature review is to evaluate the efficacy of microneedling treatment with injectable platelet-rich fibrin(i-PRF)for facial skin rejuvenation applications,using an objective skin analysis system...Aim:The aim of this literature review is to evaluate the efficacy of microneedling treatment with injectable platelet-rich fibrin(i-PRF)for facial skin rejuvenation applications,using an objective skin analysis system and validated patient-reported outcome measures.Methods:The search approach involved the exploration of electronic databases.An advanced search option was applied to filter our search line,i.e.,from February 2011 to April 2021.We performed a search on Medline,Scopus,Embase,and Web of Science,while improving the accessed articles via Ovid interface.Our keywords were chiefly aligned with a combination of MeSH terms and text words.All retrieved articles were written in English.Results:The search yielded 73 studies.After reviewing their title and summary,nine of them were found to meet the inclusion criteria and,next,the full-text articles were reviewed.Of these,three studies were excluded from systematic research,as they would no longer meet the inclusion criteria.In total,six studies were considered for review.Conclusion:Microneedling treatments combined with blood concentrates are increasingly being utilized as autologous products for aesthetic purposes.Few works can be found on i-PRF in facial rejuvenation,and even fewer on i-PRF along with microneedling.Combined applications seem to be promising and minimally invasive.Further research on PRP and PRF is warranted to better elucidate their functional roles in medical cosmetic rejuvenation.展开更多
Microneedle(MN)is a medical device containing an array of needles with a micrometer-scale.It can penetrate the human stratum corneum painlessly and efficiently for treatment and diagnosis purposes.Currently,the materi...Microneedle(MN)is a medical device containing an array of needles with a micrometer-scale.It can penetrate the human stratum corneum painlessly and efficiently for treatment and diagnosis purposes.Currently,the materials commonly used to manufacture MNs include silicon,polymers,ceramics and metals.Metallic MNs(MMNs)have drawn significant attention owing to its superior mechanical properties,machinability,and biocompatibility.This paper is a state-of-the-art review of the structure,fabrication technologies,and applications of MMNs.According to the relative position of the axis of MN and the plane of the substrate,MMNs can be divided into in-plane and out-of-plane.Solid,hollow,coated and porous MMNs are also employed to characterize their internal and surface structures.Until now,numerous fabrication technologies,including cutting tool machining,non-traditional machining,etching,hot-forming,and additive manufacturing,have been used to fabricate MMNs.The recent advances in the application of MMNs in drug delivery,disease diagnosis,and cosmetology are also discussed in-depth.Finally,the shortcomings in the fabrication and application of MMNs and future directions for development are highlighted.展开更多
The use of microneedles(MNs)has been established as an effective transdermal drug delivery strategy that has been extensively deployed for treating various diseases,including skin diseases.MNs can surpass the constrai...The use of microneedles(MNs)has been established as an effective transdermal drug delivery strategy that has been extensively deployed for treating various diseases,including skin diseases.MNs can surpass the constraints of conventional drug delivery methods by their superior safety and efficacy through precise targeting,while simultaneously enabling painless delivery.Currently,MNs are increasingly used as carriers for drug delivery,with the loading of insoluble drugs to improve their treatment efficiency or combining with bioactive substances for the construction of an efficient drug delivery system to maximize the effects of bioactive substances.The methods used for preparation MNs are diverse,enabling them to meet the requirements of most applications.The emergence of MNs has addressed the shortcomings associated with insoluble drugs,expanded the applications of bioactive substances,and improved their use in clinical practice.This review summarizes current information on the application of MNs in a variety of skin diseases,such as psoriasis,vitiligo,alopecia,hypertrophic scarring,atopic dermatitis,melanoma,acne,and skin infections.The current clinical applications and future opportunities for MNs in the treatment of skin diseases are also discussed.Despite substantial progress in the clinical application of MNs as delivery vectors,issues such as low drug loading and poor mechanical strength during MNs preparation remain the main challenges.Therefore,clinical implementation of MNs-based therapies remains limited,highlighting key opportunities for future research.展开更多
Cancer vaccines are a notable area of immunotherapy due to their capacity to elicit specific antitumor immune responses and to create immune memory.However,they encounter challenges in clinical practice due to several...Cancer vaccines are a notable area of immunotherapy due to their capacity to elicit specific antitumor immune responses and to create immune memory.However,they encounter challenges in clinical practice due to several bottlenecks,including tumor heterogeneity,low immunogenicity,immunosuppressive tumor environment,and delivery obstacles,which collectively impact their clinical effectiveness.In this study,we developed nanocomposites containing positively charged melittin(MEL)and negatively charged photosensitizer indocyanine green(ICG),embedded in dissolving microneedles(MEL/ICG-HA@DMNs).This approach allows precise drug delivery by creating microchannels that bypass the stratum corneum barrier,targeting superficial lesions directly.Our results demonstrated that the complexation of MEL and ICG significantly reduced the hemolytic activity of MEL while maintaining its ability to disrupt cell membranes.After loading MEL/ICG-HA into the microneedle,MEL/ICG-HA@DMNs not only effectively concentrated the drug at the tumor site,inducing localized hyperthermia and successfully ablating the tumor,but also formed an in situ whole-cell vaccine containing a rich source of tumor-associated antigens.Moreover,the system promoted dendritic cell maturation and increased the M1/M2 macrophage ratio,enhancing the immune response.By overcoming the limitations of traditional cancer vaccines,this system ensures precise drug delivery and robust immune activation.This innovative approach holds the potential to revolutionize cancer treatment,offering a new paradigm in precision oncology.展开更多
Bacterial infection is a major threat to global public health,and can cause serious diseases such as bacterial skin infection and foodborne diseases.It is essential to develop a new method to rapidly diagnose clinical...Bacterial infection is a major threat to global public health,and can cause serious diseases such as bacterial skin infection and foodborne diseases.It is essential to develop a new method to rapidly diagnose clinical multiple bacterial infections and monitor food microbial contamination in production sites in real-time.In this work,we developed a 4-mercaptophenylboronic acid gold nanoparticles(4-MPBA-AuNPs)-functionalized hydrogel microneedle(MPBA-H-MN)for bacteria detection in skin interstitial fluid.MPBA-H-MN could conveniently capture and enrich a variety of bacteria within 5 min.Surface enhanced Raman spectroscopy(SERS)detection was then performed and combined with machine learning technology to distinguish and identify a variety of bacteria.Overall,the capture efficiency of this method exceeded 50%.In the concentration range of 1×10_(7) to 1×10^(10) colony-forming units/mL(CFU/mL),the corresponding SERS intensity showed a certain linear relationship with the bacterial concentration.Using random forest(RF)-based machine learning,bacteria were effectively distinguished with an accuracy of 97.87%.In addition,the harmless disposal of used MNs by photothermal ablation was convenient,environmentally friendly,and inexpensive.This technique provided a potential method for rapid and real-time diagnosis of multiple clinical bacterial infections and for monitoring microbial contamination of food in production sites.展开更多
Microneedle(MN)patches could be a promising treatment for diabetic foot ulcers that plague thousands of people worldwide.While reducing skin resistance or increasing driving force can accelerate the efficiency of tran...Microneedle(MN)patches could be a promising treatment for diabetic foot ulcers that plague thousands of people worldwide.While reducing skin resistance or increasing driving force can accelerate the efficiency of transdermal drug delivery with conventional MN patches,it can create toxic chemical residues or require the help of additional devices.Herein,a thermo-responsive microneedles patch(TMN)with high biocompatibility without additional equipment is proposed.The TMN consisted of a bilayer microneedles composed of sodium alginate(SA)-g-poly(N-isopropylacrylamide)layer(SA-g-PNIPAM)loaded with sucrose octasulfate sodium salt(SOS)and hyaluronic acid layer and a polycaprolactone/chitosan nanofiber membrane loading with tetracycline hydrochloride(TH)and SOS.PNIPAM accelerates drug release by extruding the drug through a volumetric phase transition in response to temperature changes,and TH and SOS promote wound healing by inhibiting bacterial growth and promoting vascular regeneration and epithelial formation.The results showed that the drug release of TMN was significantly faster,with the drug release rate of more than 80% in the 10th h,and the antibacterial rate of TMN could reach 800%.In addition,TMN had good biocompatibility and good healing effects in vivo,which may be helpful for the design of multifunctional dressings in the future.展开更多
Microneedle-mediated drug delivery systems(MDDS)have experienced robust growth in recent years,with designers leveraging their creativity to apply these systems for direct drug delivery to the skin,mucous membranes,bl...Microneedle-mediated drug delivery systems(MDDS)have experienced robust growth in recent years,with designers leveraging their creativity to apply these systems for direct drug delivery to the skin,mucous membranes,blood vessel walls and even internal organs.In order to achieve precise drug delivery,various delicately conceived drug release modes based on MDDS have been developed.Herein,to elucidate the design concepts of numerous reported MDDS,we have categorized them into two levels(Level-ⅠMDDS and Level-ⅡMDDS)depending on whether nanoscale and microscale carriers are integrated within the microneedles.In this work,the design strategies of MDDS,as well as the current status of their applications in targeted and intelligent drug delivery were reviewed,while their prospects and challenges for future industrialization and clinical applications were also discussed.展开更多
Psoriasis is a chronic inflammatory skin disease,which seriously affects the physical and mental health of patients.The progression of psoriasis is influenced by the excessive production of reactive oxygen species(ROS...Psoriasis is a chronic inflammatory skin disease,which seriously affects the physical and mental health of patients.The progression of psoriasis is influenced by the excessive production of reactive oxygen species(ROS)and inflammatory responses.In this paper,novel celastrol(Ce)-loaded metal-phenolic nanozymes(tannic acid-Fe^(3+))(TA-Fe)integrated microneedles(Ce@TA-Fe/MNs)were constructed to achieve the combined oxidative stress alleviation and anti-inflammatory therapy of psoriasis.Molecular dynamics simulations and structural characterization confirmed the successful fabrication of nanozymes.The Ce@TA-Fe/MNs system,characterized by its rapid dissolution kinetics and superior mechanical strength,enabled minimally invasive skin penetration for efficient nanozymes delivery.Nanozymes possessed superoxide dismutase and catalase mimetic enzyme activities,effectively eliminating excessive ROS in psoriatic skin lesions.Additionally,the release of Ce from Ce@TA-Fe provided strong antioxidant and anti-inflammatory effects.Based on these characteristics,Ce@TA-Fe/MNs could effectively alleviate the symptoms in psoriasis mice models.These findings demonstrated that the integration of Ce-equipped nanozymes within MNs holds great promise as a therapeutic strategy for the clinical management of psoriasis.展开更多
Acne vulgaris is one of the most common skin disorders affecting millions of patients worldwide,its long-lasting inflammation greatly reduces life quality and causes negative psychosocial impacts.Conventional treatmen...Acne vulgaris is one of the most common skin disorders affecting millions of patients worldwide,its long-lasting inflammation greatly reduces life quality and causes negative psychosocial impacts.Conventional treatments often along with side effects and issues of patient compliance,and ineffective in treating severe conditions.In recent years,microneedle(MN)has emerged as a versatile therapeutic technology,owing to its minimally invasive,effective,and reduced side effects.However,there are few review articles that systematically summarize the progress of microneedles for the treatment of acne.Here conclude the material,function,and application of microneedle technology in the treatment of acne,with a particular focus on two types of anti-acne microneedle:drug-loaded microneedle(DMN)and radio-frequency microneedle(RMN).DMN facilitates targeted drug delivery to the skin's deeper layers,while RMN utilizes radio-frequency currents to stimulate collagen regeneration,thus addressing acne scarring.Additionally,future directions for advanced acne-treating microneedle technology are envisioned,such as diversified drug loading,multi-functionality,production process optimization,and personalized treatment.These different directions are expected to further enhance the safety,efficacy,and patient satisfaction of microneedle acne treatments.展开更多
Microneedles(MNs)offer a precise and minimally invasive platform for delivering vaccines and therapeutic agents directly into the skin,leveraging the abundance of tissue-resident immune cells to elicit robust and dura...Microneedles(MNs)offer a precise and minimally invasive platform for delivering vaccines and therapeutic agents directly into the skin,leveraging the abundance of tissue-resident immune cells to elicit robust and durable immune responses.Compared to traditional intramuscular or subcutaneous vaccination methods,MNbased vaccines demonstrate superior patient compliance,enhanced antigen stability,and heightened immunogenicity,positioning them as a promising tool in biomedical applications.This review provides a comprehensive overview of the materials and fabrication techniques used in MN preparation,explores their structural classifications,and examines the role of antigens and adjuvants in optimizing vaccine efficacy.Furthermore,the diverse applications of MN delivery systems in preventing infectious diseases,advancing tumor immunotherapy,and addressing other immune-related conditions are discussed.展开更多
Simultaneously suppressing tumor growth and metastasis is a pivotal strategy in the treatment of cutaneous melanoma(CM).Towards this end,we first developed a novel PtCu nanozyme(PtCu-zyme)integrating single-atom Pt an...Simultaneously suppressing tumor growth and metastasis is a pivotal strategy in the treatment of cutaneous melanoma(CM).Towards this end,we first developed a novel PtCu nanozyme(PtCu-zyme)integrating single-atom Pt and Pt subnanoclusters,which was further functionalized with triphenylphosphine(TPP)to yield PtCu-TPP and confer the nanozyme mitochondria-targeting capabilities.By combining PtCuTPP with a hyaluronic acid(HA)analog,isoliquiritigenin-grafted HA(HA-ISL),we later formulated PtCuTPP loaded microneedles(PtCu-TPP@MNs)for potent CM treatment.Our findings indicated that PtCuzyme exhibited exceptional oxidative enzyme-like properties and PtCu-TPP@MNs significantly inhibited the tumor growth and pulmonary metastasis.Furthermore,PtCu-TPP@MNs not only prolonged the survival of CM-bearing mice but also retained the nanozymes in the tumor,continually catalyzing reactive oxygen species(ROS)generation for sustained nanocatalytic therapy.In vitro studies revealed that PtCuTPP specifically localized within mitochondria,increasing ROS levels and causing mitochondrial damage,which in turn enhanced the cytotoxicity towards tumor cells.These findings suggest that PtCu-TPP@MN delivery system holds significant promise for the effective treatment of CM,potentially offering a valuable alternative to existing therapeutic strategies.展开更多
文摘Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,including reducing inflammatory response,improving immunity and enhancing antioxidant levels.In this prospective randomized double-blinded study,we wanted to explore the clinical efficacy and safety of Kangfuxin liquid combined with microneedling in treating facial skin photoaging.Methods:57 patients with facial skin photoaging were randomly divided into two groups.The treatment group(28 cases)received microneedle therapy with Kangfuxin liquid,while the control group(29 cases)received microneedle therapy with physiological saline.The treatment interval was 4 weeks,and a total of 3 treatments were performed.Compare the VISIA scores of facial photoaging features such as wrinkles,texture,pores,spots and ultraviolet pigmentation between two groups of patients before and after treatment,Global Score for Photoaging,satisfaction evaluation,and record the occurrence of adverse reactions.Results:After treatment,the treatment group showed more significant improvement in wrinkles,texture,pores,spots and ultraviolet pigmentation,and the Global Score for Photoaging was better than the control group(P<0.05).The satisfaction rate with improving skin in the treatment group was 85.71%,which was higher than 75.86%in the control group(P<0.05).Both groups did not experience adverse reactions such as skin infection,pigmentation or hypopigmentation,scar formation,or worsening of melasma.Conclusion:Kangfuxin liquid combined with microneedling therapy has a good improvement effect on facial skin photoaging,with a low incidence of adverse reactions and high patient satisfaction.It is worthy of clinical promotion.
基金the Beijing Science and Technology New Star Program(grant No.2015A048)the Young Teacher Special of Beijing University of Chinese Medicine(1000061221025)National Science and Technology Major New Drug Projects(2014ZX09301306-009).
文摘Objective:To combine with transdermal drug delivery using microneedle to simulate the bee venom therapy to evaluate the permeation of bee venom gel.Methods:In this study,the sodium urate and LPS were used on rats and mice to construct the model.Bee venom gelemicroneedle combination effect on the model is to determine the role of microneedle gel permeation by observing inflammation factors.Results:Compared with the model group,the bee venom gelemicroneedle combination group can reduce the level of serum nitric oxide of the acute gouty inflammation model caused by sodium urate,and on LPS induced mouse model of acute inflammation effect and the micro.Conclusions:Bee venom can significantly suppress the occurrence of gouty arthritis inflammation in rats and mice LPS inflammatory reaction.Choose the 750 mm microneedle with 10N force on skin about 3 minutes,bee venom can play the optimal role,and the anti-inflammatory effect is obvious.Microneedles can promote the percutaneous absorption of the active macromolecules bee venom gel.
文摘Objective:Current treatments for atrophic acne scars such as microneedling radiofrequency(MNRF)and fractional carbon dioxide(FCO_(2))laser have shown individual efficacy,but their combined effectiveness remains lacking.This study aimed to determine the effectiveness and safety of a sequential combination therapy with MNRF followed by FCO_(2) laser compared with each therapy administered alone for the treatment of atrophic acne scars.Methods:An open randomized trial was conducted on 45 patients with atrophic acne scars at Al-Salam Teaching Hospital,Mosul,Iraq,from January 2022 to July 2023.The patients were randomized into 3 groups,with 15 patients in each group:FCO_(2) group(6 sessions of FCO_(2) laser treatment),MNRF group(6 sessions of MNRF treatment),and combined group(3 sessions of MNRF treatment followed by 3 sessions of FCO_(2) laser treatment).The Goodman and Baron Global Quantitative Acne Scar Grading System(GBGQASGS)was used to assess the treatment effectiveness.Adverse effects of treatment were monitored.The pre-treatment and post-treatment GBGQASGS scores and the percentage reductions in the GBGQASGS scores in the 3 groups were assessed by analysis of variance.The effect size of each treatment regimen was assessed using the Cohen d effect size.Results:The 45 patients enrolled comprised 20(44.4%)men and 25(55.6%)women,with a mean age of 25.10±2.80 years and a GBGQASGS score of 28.93±6.19.All 3 types of treatment significantly reduced the GBGQASGS scores after 6 sessions(all P<0.05)as following:FCO_(2) group,from 28.66±5.71 to 11.66±2.71(59.3%reduction;Cohen d=3.80);MNRF group,from 27.26±6.93 to 11.66±3.09(57.2%reduction;Cohen d=2.90);Combined group,from 30.87±5.73 to 9.01±2.03(70.8%reduction;Cohen d=5.08).There was no difference in the GBGQASGS score of post-treatment among 3 groups(P=0.300),however,the combined group exhibited a significant reduction in the GBGQASGS score from pre-treatment to post-treatment(P<0.001).Post-inflammatory hyperpigmentation was reported in FCO_(2) and MNRF groups,tram-track scarring was found in FCO_(2) group,while no serious adverse effects were reported in the combined group.Conclusion:Sequential combination therapy with MNRF followed by FCO_(2) laser was as effective as each monotherapy for the treatment of atrophic acne scars with fewer adverse effects.The results may provide evidence for treatment choice.
基金supported by National Research Foundation of Korea(NRF)grants funded by the Korean government(MSIT)(No.RS-2023-00256265,RS-2024-00352352,RS-2024-00405818)the Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korea government(the Ministry of Science and ICT,the Ministry of Health&Welfare).(No.25A0102L1)support from the Market-led K-sensor technology program(RS-2022-00154781,Development of large-area wafer-level flexible/stretchable hybrid sensor platform technology for form factor-free highly integrated convergence sensor),funded By the Ministry of Trade,Industry&Energy(MOTIE,Korea).
文摘Microneedles(MNs)have been extensively investigated for transdermal delivery of large-sized drugs,including proteins,nucleic acids,and even extracellular vesicles(EVs).However,for their sufficient skin penetration,conventional MNs employ long needles(≥600μm),leading to pain and skin irritation.Moreover,it is critical to stably apply MNs against complex skin surfaces for uniform nanoscale drug delivery.Herein,a dually amplified transdermal patch(MN@EV/SC)is developed as the stem cell-derived EV delivery platform by hierarchically integrating an octopusinspired suction cup(SC)with short MNs(≤300μm).While leveraging the suction effect to induce nanoscale deformation of the stratum corneum,MN@EV/SC minimizes skin damage and enhances the adhesion of MNs,allowing EV to penetrate deeper into the dermis.When MNs of various lengths are applied to mouse skin,the short MNs can elicit comparable corticosterone release to chemical adhesives,whereas long MNs induce a prompt stress response.MN@EV/SC can achieve a remarkable penetration depth(290μm)for EV,compared to that of MN alone(111μm).Consequently,MN@EV/SC facilitates the revitalization of fibroblasts and enhances collagen synthesis in middle-aged mice.Overall,MN@EV/SC exhibits the potential for skin regeneration by modulating the dermal microenvironment and ensuring patient comfort.
基金supported by the National Key R esearch and Development Program of China(No.2023YFF0724300)the National Natural Science Foundation of China(No.32171373)+1 种基金the Fundamental Research Funds for the Central Universities(No.YG2025QNB08)the Natural Science Foundation of Shanghai(No.23ZR1414500).
文摘Microneedle technology has undergone a paradigm shift from basic transdermal drug delivery to intelligent,closed-loop theranostic systems.Hydrogel materials have emerged as core carriers due to their excellent biocompatibility,efficient drug loading capacity,and improved patient compliance.Moreover,critical bottlenecks in hydrogel microneedles,including poor mechanical strength,burst release of drugs,and delayed response to treatment,can be addressed via cross-scale integration of nanomaterials.This review systematically outlines several multiscale engineering strategies to overcome these limitations.The construction of nanotopological networks coupled with dynamic crosslinking modulation synergistically enhances the mechanical properties,stability of drug loading,and conductivity of hydrogel microneedles.Furthermore,responsive nanocarriers equipped with biosensors help establish a closed-loop linkage between monitoring and therapeutic functions.We highlight their synergistic theranostic advantages in scenarios such as wound regulation and tumor-immune microenvironments,while revealing the role in integrating flexible electronics with wearable systems in intelligent medicine.We also summarize the research advances on the biosafety and scalable manufacturing processes of nanocomposite hydrogel m icroneedles(NHMNs),providing examples of clinical translation to elucidate the path from fundamental research to industrial implementation.As a convergence of nanotechnology,biomaterials,and flexible electronics,NHMNs provide new standards for transdermal theranostics as well as a roadmap for iterative advancement of intelligent theranostic devices in personalized medicine.Their cross-scale collaborative design,which spans from the properties of materials to the functional integration of macroscopic devices,can facilitate potential breakthroughs in next-generation closed-loop theranostic systems.
基金the financial support from the Fujian Provincial Youth Top-Notch Talent Support Program,China.
文摘The clinical management of hypertrophic scars(HSs)remains challenging due to their complex etiology and heterogeneous morphology,underscoring the need for multitarget treatment strategies.In this study,we developed a nanocomposite system constructed through the metal-phenolic network-mediated self-assembly of molybdenum polyoxometalate({Mo 154})and epigallocatechin gallate(EGCG),followed by chitosan encapsulation,to generate chitosan-encapsulated{Mo 154}/EGCG(CME)nanoparticles.These nanoparticles were integrated into dissolvable microneedles(CME@MN)to enable transdermal administration.Under near-infrared laser irradiation,CME exhibited a three-pronged therapeutic effect:suppression of collagen overproduction and excessive extracellular matrix(ECM)deposition in human keloid fibroblasts,regulation of proliferation and migration in human umbilical vein endothelial cells,and reprogramming of macrophages toward a proinflammatory M1 phenotype.In vivo,CME@MN patches preferentially accumulated within scar tissue,where they normalized ECM organization,improved collagen fiber rearrangement,and attenuated fibroblast activity through photothermal-enhanced mechanisms while maintaining an excellent safety profile.The CME@MN system represents a potentially transformative approach to HS management by offering a unified platform that simultaneously targets the fibrotic,angiogenic,and inflammatory components of scar pathogenesis.
文摘Biofabrication and biomanufacturing are rapidly transforming how materials,therapeutics,and functional biological constructs are produced.These fields integrate developments in sustainable biomaterials,precision fabrication,biological systems,and data-driven engineering to produce scalable,efficient,and environmentally aligned production pathways.This review highlights recent scientific advances led by researchers in Singapore,focusing on three interconnected pillars:sustainable bio derived materials,enabling fabrication and manufacturing technologies,and emerging applications.We first examine the expanding use of biomass-derived feedstocks,including human hair keratin,aquaculture side-streams,and plant-derived polysaccharides,which support circular and resource-conscious material development.We then present advances in biofabrication technologies,including electrospinning,three-dimensional bioprinting,and metal additive manufacturing,that enable improved control over the structure,function,and manufacturability of biomedical and functional constructs.Emerging applications,such as machine learning-assisted additive manufacturing,food biomanufacturing,regenerative cell therapy,microneedles,and bioelectronics,exemplify how biofabrication and biomanufacturing are increasingly interrelated across the health,materials,and technological domains.These research contributions from Singapore exemplify how sustainable feedstocks,digital and automated fabrication platforms,and biologically driven applications are shaping the evolving landscape of biofabrication and biomanufacturing.The convergence of materials science,biological engineering,and advanced manufacturing continues to enable new opportunities for innovation in biomedical,industrial,and societal contexts.
基金supported in part by the National Key R&D Program of China(No.2023YFB4705801)the Progect of Medical Engineering Laboratory of Chinese PLA General Hospital(Grant No.2022SYSZZKY13)+1 种基金the National Natural Science Foundation of China(Nos.52175008 and 92048301)the National Outstanding Youth Science Fund Project of National Natural Science Foundation of China(Grant No.52025054)。
文摘Subretinal injection(SI)is recognized as the most effective method for treating retinal degenerative and genetic diseases,enabling targeted delivery of therapeutic agents such as viral vectors or stem cells directly into the subretinal space(SRS).However,this procedure is typically performed under extremely constrained intraocular conditions,characterized by limited visualization,lack of tactile feedback,and the inherent fragility of retinal tissues,significantly increasing the risk of surgical complications and potential vision impairment.To overcome these challenges,an integrated force-sensing microneedle equipped with a flexible joint was developed and integrated into an ophthalmic surgical robotic system.This advanced system provides precise needle-tip pose control and real-time puncture force feedback.Leveraging these capabilities,force-guided autonomous retinal puncture was achieved,ensuring accurate and consistent drug delivery into the SRS.Comparative experiments conducted on live Bama pigs demonstrated the robotic system's superiority over manual SI techniques,as evidenced by more stable puncture trajectories,smoother insertion velocities,precise insertion depths,and a greater than 90%reduction in average puncture force,despite respiratory and cardiac-induced disturbances,thus significantly enhancing surgical precision and minimizing the risk of iatrogenic retinal injury.Postoperative evaluations further validated that the robotic approach markedly reduced drug reflux into the vitreous cavity,underscoring its reliability and safety for SI.
基金financial support from the National Key Research and Development Program of China(2024YFA0919100)the National Natural Science Foundation of China(32371435)+2 种基金the Qinglan Project of Jiangsu Province(2025 Excellent Young Scholar,Bingbing Gao)the Jiangsu government scholarship for overseas studies(Bingbing Gao)the Nanjing Tech University Teaching Reform Project(20250281)。
文摘Soft robots,characterized by compliance,adaptability,and multimodal responsiveness,represent a rapidly advancing frontier in biomedical applications,wearable technologies,and environmental exploration.This review summarizes recent progress in soft robotics with a focus on material innovation,structural design,functional integration,and intelligent responsiveness.Emphasis is placed on the development of bioinspired and stimuli-responsive materials,the construction of modular and reconfigurable architectures,and the integration of actuation,sensing,and energy systems.Microneedle array-based soft robots and hydrogel-based 4D-printed systems are introduced as representative platforms for drug delivery,wound healing,and environmental monitoring.Key challenges,including limited durability,power autonomy,and multifunctional synergy,are critically analyzed in relation to practical operation and long-term reliability.Future directions involve the convergence of self-healing materials,intelligent control algorithms,and multiscale integration strategies to achieve enhanced adaptability and clinical translation.This review provides a comprehensive overview of the interdisciplinary development of next-generation soft robots that bridge materials science,biomedical engineering,and intelligent systems,paving the way toward real-world applications.
基金supported by an institutional grant of the University of Camerino.
文摘Aim:The aim of this literature review is to evaluate the efficacy of microneedling treatment with injectable platelet-rich fibrin(i-PRF)for facial skin rejuvenation applications,using an objective skin analysis system and validated patient-reported outcome measures.Methods:The search approach involved the exploration of electronic databases.An advanced search option was applied to filter our search line,i.e.,from February 2011 to April 2021.We performed a search on Medline,Scopus,Embase,and Web of Science,while improving the accessed articles via Ovid interface.Our keywords were chiefly aligned with a combination of MeSH terms and text words.All retrieved articles were written in English.Results:The search yielded 73 studies.After reviewing their title and summary,nine of them were found to meet the inclusion criteria and,next,the full-text articles were reviewed.Of these,three studies were excluded from systematic research,as they would no longer meet the inclusion criteria.In total,six studies were considered for review.Conclusion:Microneedling treatments combined with blood concentrates are increasingly being utilized as autologous products for aesthetic purposes.Few works can be found on i-PRF in facial rejuvenation,and even fewer on i-PRF along with microneedling.Combined applications seem to be promising and minimally invasive.Further research on PRP and PRF is warranted to better elucidate their functional roles in medical cosmetic rejuvenation.
基金Supported by Guangdong Provincial Key-Area Research and Development Program(Grant No.2023B0101200014)Guangdong Provincial Natural Science Foundation(Grant No.2024A1515010440).
文摘Microneedle(MN)is a medical device containing an array of needles with a micrometer-scale.It can penetrate the human stratum corneum painlessly and efficiently for treatment and diagnosis purposes.Currently,the materials commonly used to manufacture MNs include silicon,polymers,ceramics and metals.Metallic MNs(MMNs)have drawn significant attention owing to its superior mechanical properties,machinability,and biocompatibility.This paper is a state-of-the-art review of the structure,fabrication technologies,and applications of MMNs.According to the relative position of the axis of MN and the plane of the substrate,MMNs can be divided into in-plane and out-of-plane.Solid,hollow,coated and porous MMNs are also employed to characterize their internal and surface structures.Until now,numerous fabrication technologies,including cutting tool machining,non-traditional machining,etching,hot-forming,and additive manufacturing,have been used to fabricate MMNs.The recent advances in the application of MMNs in drug delivery,disease diagnosis,and cosmetology are also discussed in-depth.Finally,the shortcomings in the fabrication and application of MMNs and future directions for development are highlighted.
基金supported through grants from the Key Fields of Biomedicine and Health Foundation of Colleges and Universities in Guangdong Province(2022ZDZX2017)the National Natural Science Foundation of China(82104354)+1 种基金the Guangdong Basic and Applied Basic Research Foundation(2022A1515012154)the funding grants from University of Macao and the University of Macao Development Foundation(MYRG2023-GRG00184-ICMS-UMDF and MYRG2024-GRG00271-ICMS-UMDF).
文摘The use of microneedles(MNs)has been established as an effective transdermal drug delivery strategy that has been extensively deployed for treating various diseases,including skin diseases.MNs can surpass the constraints of conventional drug delivery methods by their superior safety and efficacy through precise targeting,while simultaneously enabling painless delivery.Currently,MNs are increasingly used as carriers for drug delivery,with the loading of insoluble drugs to improve their treatment efficiency or combining with bioactive substances for the construction of an efficient drug delivery system to maximize the effects of bioactive substances.The methods used for preparation MNs are diverse,enabling them to meet the requirements of most applications.The emergence of MNs has addressed the shortcomings associated with insoluble drugs,expanded the applications of bioactive substances,and improved their use in clinical practice.This review summarizes current information on the application of MNs in a variety of skin diseases,such as psoriasis,vitiligo,alopecia,hypertrophic scarring,atopic dermatitis,melanoma,acne,and skin infections.The current clinical applications and future opportunities for MNs in the treatment of skin diseases are also discussed.Despite substantial progress in the clinical application of MNs as delivery vectors,issues such as low drug loading and poor mechanical strength during MNs preparation remain the main challenges.Therefore,clinical implementation of MNs-based therapies remains limited,highlighting key opportunities for future research.
基金supported by the National Natural Science Foundation of China(Nos.82173747,82373803)the Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province(No.2021TQ060944)。
文摘Cancer vaccines are a notable area of immunotherapy due to their capacity to elicit specific antitumor immune responses and to create immune memory.However,they encounter challenges in clinical practice due to several bottlenecks,including tumor heterogeneity,low immunogenicity,immunosuppressive tumor environment,and delivery obstacles,which collectively impact their clinical effectiveness.In this study,we developed nanocomposites containing positively charged melittin(MEL)and negatively charged photosensitizer indocyanine green(ICG),embedded in dissolving microneedles(MEL/ICG-HA@DMNs).This approach allows precise drug delivery by creating microchannels that bypass the stratum corneum barrier,targeting superficial lesions directly.Our results demonstrated that the complexation of MEL and ICG significantly reduced the hemolytic activity of MEL while maintaining its ability to disrupt cell membranes.After loading MEL/ICG-HA into the microneedle,MEL/ICG-HA@DMNs not only effectively concentrated the drug at the tumor site,inducing localized hyperthermia and successfully ablating the tumor,but also formed an in situ whole-cell vaccine containing a rich source of tumor-associated antigens.Moreover,the system promoted dendritic cell maturation and increased the M1/M2 macrophage ratio,enhancing the immune response.By overcoming the limitations of traditional cancer vaccines,this system ensures precise drug delivery and robust immune activation.This innovative approach holds the potential to revolutionize cancer treatment,offering a new paradigm in precision oncology.
基金supported by the National Natural Science Foundation of China(Grant Nos.:82204340,82173954,and 82073815)the Natural Science Foundation of Jiangsu Province,China(Grant No.:BK20221048)+1 种基金the Jiangsu Funding Program for Excellent Postdoctoral Talent,China(Grant No.:2022ZB295)Key Laboratory Project of Quality Control of Chinese Herbal Medicines and Decoction Pieces,Gansu Institute for Drug Control,China(Grant No.:2024GSMPA-KL02).
文摘Bacterial infection is a major threat to global public health,and can cause serious diseases such as bacterial skin infection and foodborne diseases.It is essential to develop a new method to rapidly diagnose clinical multiple bacterial infections and monitor food microbial contamination in production sites in real-time.In this work,we developed a 4-mercaptophenylboronic acid gold nanoparticles(4-MPBA-AuNPs)-functionalized hydrogel microneedle(MPBA-H-MN)for bacteria detection in skin interstitial fluid.MPBA-H-MN could conveniently capture and enrich a variety of bacteria within 5 min.Surface enhanced Raman spectroscopy(SERS)detection was then performed and combined with machine learning technology to distinguish and identify a variety of bacteria.Overall,the capture efficiency of this method exceeded 50%.In the concentration range of 1×10_(7) to 1×10^(10) colony-forming units/mL(CFU/mL),the corresponding SERS intensity showed a certain linear relationship with the bacterial concentration.Using random forest(RF)-based machine learning,bacteria were effectively distinguished with an accuracy of 97.87%.In addition,the harmless disposal of used MNs by photothermal ablation was convenient,environmentally friendly,and inexpensive.This technique provided a potential method for rapid and real-time diagnosis of multiple clinical bacterial infections and for monitoring microbial contamination of food in production sites.
基金supported by the Joint Funds of National Natural Science Foundation of China(No.U22A20162)the Natural Science Foundation of Hebei Province of China(No.C2021202002)+1 种基金the National Natural Science Foundation of China(No.52271245),the Natural Science Foundation of Tianjin(No.21JCQNJC01280)the financial support from the Danish Council for Independent Research(9040-00219B),European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement ENSIGN(Project ID:101086226),L4DNANO(Project ID:101086227).
文摘Microneedle(MN)patches could be a promising treatment for diabetic foot ulcers that plague thousands of people worldwide.While reducing skin resistance or increasing driving force can accelerate the efficiency of transdermal drug delivery with conventional MN patches,it can create toxic chemical residues or require the help of additional devices.Herein,a thermo-responsive microneedles patch(TMN)with high biocompatibility without additional equipment is proposed.The TMN consisted of a bilayer microneedles composed of sodium alginate(SA)-g-poly(N-isopropylacrylamide)layer(SA-g-PNIPAM)loaded with sucrose octasulfate sodium salt(SOS)and hyaluronic acid layer and a polycaprolactone/chitosan nanofiber membrane loading with tetracycline hydrochloride(TH)and SOS.PNIPAM accelerates drug release by extruding the drug through a volumetric phase transition in response to temperature changes,and TH and SOS promote wound healing by inhibiting bacterial growth and promoting vascular regeneration and epithelial formation.The results showed that the drug release of TMN was significantly faster,with the drug release rate of more than 80% in the 10th h,and the antibacterial rate of TMN could reach 800%.In addition,TMN had good biocompatibility and good healing effects in vivo,which may be helpful for the design of multifunctional dressings in the future.
基金supported by the National Natural Science Foundation of China(Grant number 82074031)the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning(Grant number TP2020054)+1 种基金Shanghai Sailing Program(21YF1447400)the Program for Shanghai High-Level Local University Innovation Team(SZY20220315).
文摘Microneedle-mediated drug delivery systems(MDDS)have experienced robust growth in recent years,with designers leveraging their creativity to apply these systems for direct drug delivery to the skin,mucous membranes,blood vessel walls and even internal organs.In order to achieve precise drug delivery,various delicately conceived drug release modes based on MDDS have been developed.Herein,to elucidate the design concepts of numerous reported MDDS,we have categorized them into two levels(Level-ⅠMDDS and Level-ⅡMDDS)depending on whether nanoscale and microscale carriers are integrated within the microneedles.In this work,the design strategies of MDDS,as well as the current status of their applications in targeted and intelligent drug delivery were reviewed,while their prospects and challenges for future industrialization and clinical applications were also discussed.
基金supported by Key Research Project of the Educational Department of Liaoning Province,China(JYTZD2023139).
文摘Psoriasis is a chronic inflammatory skin disease,which seriously affects the physical and mental health of patients.The progression of psoriasis is influenced by the excessive production of reactive oxygen species(ROS)and inflammatory responses.In this paper,novel celastrol(Ce)-loaded metal-phenolic nanozymes(tannic acid-Fe^(3+))(TA-Fe)integrated microneedles(Ce@TA-Fe/MNs)were constructed to achieve the combined oxidative stress alleviation and anti-inflammatory therapy of psoriasis.Molecular dynamics simulations and structural characterization confirmed the successful fabrication of nanozymes.The Ce@TA-Fe/MNs system,characterized by its rapid dissolution kinetics and superior mechanical strength,enabled minimally invasive skin penetration for efficient nanozymes delivery.Nanozymes possessed superoxide dismutase and catalase mimetic enzyme activities,effectively eliminating excessive ROS in psoriatic skin lesions.Additionally,the release of Ce from Ce@TA-Fe provided strong antioxidant and anti-inflammatory effects.Based on these characteristics,Ce@TA-Fe/MNs could effectively alleviate the symptoms in psoriasis mice models.These findings demonstrated that the integration of Ce-equipped nanozymes within MNs holds great promise as a therapeutic strategy for the clinical management of psoriasis.
基金support from the College Students’Innovative Entrepreneurial Training Plan Program(Northwestern Polytechnical University),the National Natural Science Foundation of China(No.52473265)the Shaanxi Provincial Science Fund for Distinguished Young Scholars(No.2023-JC-JQ-32).
文摘Acne vulgaris is one of the most common skin disorders affecting millions of patients worldwide,its long-lasting inflammation greatly reduces life quality and causes negative psychosocial impacts.Conventional treatments often along with side effects and issues of patient compliance,and ineffective in treating severe conditions.In recent years,microneedle(MN)has emerged as a versatile therapeutic technology,owing to its minimally invasive,effective,and reduced side effects.However,there are few review articles that systematically summarize the progress of microneedles for the treatment of acne.Here conclude the material,function,and application of microneedle technology in the treatment of acne,with a particular focus on two types of anti-acne microneedle:drug-loaded microneedle(DMN)and radio-frequency microneedle(RMN).DMN facilitates targeted drug delivery to the skin's deeper layers,while RMN utilizes radio-frequency currents to stimulate collagen regeneration,thus addressing acne scarring.Additionally,future directions for advanced acne-treating microneedle technology are envisioned,such as diversified drug loading,multi-functionality,production process optimization,and personalized treatment.These different directions are expected to further enhance the safety,efficacy,and patient satisfaction of microneedle acne treatments.
基金supported by the National Science Fund for National Natural Science Foundation of China(Grant Nos.22232006,22377127,and 52361145848)Chinese Academy of Sciences(CAS)Project for Young Scientists in Basic Research(Grant No.YSBR-083)+2 种基金the Beijing Nova Program(Grant Nos.20230484352 and 20240484650)Institute of Process Engineering Project for Frontier Basic Research(Grant No.QYJC-2023-05)Progress of Strategy Priority Research Program(Category B)of CAS(Grant No.XDB0520300).
文摘Microneedles(MNs)offer a precise and minimally invasive platform for delivering vaccines and therapeutic agents directly into the skin,leveraging the abundance of tissue-resident immune cells to elicit robust and durable immune responses.Compared to traditional intramuscular or subcutaneous vaccination methods,MNbased vaccines demonstrate superior patient compliance,enhanced antigen stability,and heightened immunogenicity,positioning them as a promising tool in biomedical applications.This review provides a comprehensive overview of the materials and fabrication techniques used in MN preparation,explores their structural classifications,and examines the role of antigens and adjuvants in optimizing vaccine efficacy.Furthermore,the diverse applications of MN delivery systems in preventing infectious diseases,advancing tumor immunotherapy,and addressing other immune-related conditions are discussed.
基金support by the National Natural Science Foundation of China(Nos.82172591 and 81573011)。
文摘Simultaneously suppressing tumor growth and metastasis is a pivotal strategy in the treatment of cutaneous melanoma(CM).Towards this end,we first developed a novel PtCu nanozyme(PtCu-zyme)integrating single-atom Pt and Pt subnanoclusters,which was further functionalized with triphenylphosphine(TPP)to yield PtCu-TPP and confer the nanozyme mitochondria-targeting capabilities.By combining PtCuTPP with a hyaluronic acid(HA)analog,isoliquiritigenin-grafted HA(HA-ISL),we later formulated PtCuTPP loaded microneedles(PtCu-TPP@MNs)for potent CM treatment.Our findings indicated that PtCuzyme exhibited exceptional oxidative enzyme-like properties and PtCu-TPP@MNs significantly inhibited the tumor growth and pulmonary metastasis.Furthermore,PtCu-TPP@MNs not only prolonged the survival of CM-bearing mice but also retained the nanozymes in the tumor,continually catalyzing reactive oxygen species(ROS)generation for sustained nanocatalytic therapy.In vitro studies revealed that PtCuTPP specifically localized within mitochondria,increasing ROS levels and causing mitochondrial damage,which in turn enhanced the cytotoxicity towards tumor cells.These findings suggest that PtCu-TPP@MN delivery system holds significant promise for the effective treatment of CM,potentially offering a valuable alternative to existing therapeutic strategies.
