Benefiting from the great advances of the femtosecond laser two-photon polymerization(TPP)technology,customized microcantilever probes can be accurately 3-dimensional(3D)manufactured at the nanoscale size and thus hav...Benefiting from the great advances of the femtosecond laser two-photon polymerization(TPP)technology,customized microcantilever probes can be accurately 3-dimensional(3D)manufactured at the nanoscale size and thus have exhibited considerable potentials in the fields of microforce,micro-vibration,and microforce sensors.In this work,a controllable microstructured cantilever probe on an optical fiber tip for microforce detection is demonstrated both theoretically and experimentally.The static performances of the probe are firstly investigated based on the finite element method(FEM),which provides the basis for the structural design.The proposed cantilever probe is then 3D printed by means of the TPP technology.The experimental results show that the elastic constant k of the proposed cantilever probe can be actively tuned from 2.46N/m to 62.35N/m.The force sensitivity is 2.5nm/μN,the Q-factor is 368.93,and the detection limit is 57.43nN.Moreover,the mechanical properties of the cantilever probe can be flexibly adjusted by the geometric configuration of the cantilever.Thus,it has an enormous potential for matching the mechanical properties of biological samples in the direct contact mode.展开更多
The microinjection of Zebrafish embryos is significant to life science and biomedical research.In this article,a novel automated system is developed for cell microinjection.A sophisticated microfluidic chip is designe...The microinjection of Zebrafish embryos is significant to life science and biomedical research.In this article,a novel automated system is developed for cell microinjection.A sophisticated microfluidic chip is designed to transport,hold,and inject cells continuously.For the first time,a microinjector with microforce perception is proposed and integrated within the enclosed microfluidic chip to judge whether cells have been successfully punctured.The deep learning model is employed to detect the yolk center of zebrafish embryos and locate the position of the injection needle within the yolk,which enables enhancing the precision of cell injection.A prototype is fabricated to achieve automatic batch microinjection.Experimental results demonstrated that the injection efficiency is about 20 seconds per cell.Cell puncture success rate and cell survival rate are 100%and 84%,respectively.Compared to manual operation,this proposed system improves cell operation efficiency and cell survival rate.The proposed microinjection system has the potential to greatly reduce the workload of the experimenters and shorten the relevant study period.展开更多
基金supported by the Shenzhen Science and Technology Program (Grant No.RCYX20200714114524139)Shenzhen Key Laboratory of Ultrafast Laser Micro/Nano Manufacturing (Grant No.ZDSYS20220606100405013)+2 种基金Natural Science Foundation of GuangdongProvince (Grant Nos.2022B1515120061 and 2022A1515110971)National Natural Science Foundation of China (Grant Nos.62122057,62075136,62105217,and 62305223)China Postdoctoral Science Foundation (Grant No.2022M722173).
文摘Benefiting from the great advances of the femtosecond laser two-photon polymerization(TPP)technology,customized microcantilever probes can be accurately 3-dimensional(3D)manufactured at the nanoscale size and thus have exhibited considerable potentials in the fields of microforce,micro-vibration,and microforce sensors.In this work,a controllable microstructured cantilever probe on an optical fiber tip for microforce detection is demonstrated both theoretically and experimentally.The static performances of the probe are firstly investigated based on the finite element method(FEM),which provides the basis for the structural design.The proposed cantilever probe is then 3D printed by means of the TPP technology.The experimental results show that the elastic constant k of the proposed cantilever probe can be actively tuned from 2.46N/m to 62.35N/m.The force sensitivity is 2.5nm/μN,the Q-factor is 368.93,and the detection limit is 57.43nN.Moreover,the mechanical properties of the cantilever probe can be flexibly adjusted by the geometric configuration of the cantilever.Thus,it has an enormous potential for matching the mechanical properties of biological samples in the direct contact mode.
基金support from the National Natural Science Foundation of China(32101626)the Shandong Province Key R&D Plan Project(2022LZGC020)+1 种基金the Natural Science Foundation of Jiangsu Province of China(BK20220490)the Fundamental Research Funds for the Central Universities under Grant No.226-2024-00227.
文摘The microinjection of Zebrafish embryos is significant to life science and biomedical research.In this article,a novel automated system is developed for cell microinjection.A sophisticated microfluidic chip is designed to transport,hold,and inject cells continuously.For the first time,a microinjector with microforce perception is proposed and integrated within the enclosed microfluidic chip to judge whether cells have been successfully punctured.The deep learning model is employed to detect the yolk center of zebrafish embryos and locate the position of the injection needle within the yolk,which enables enhancing the precision of cell injection.A prototype is fabricated to achieve automatic batch microinjection.Experimental results demonstrated that the injection efficiency is about 20 seconds per cell.Cell puncture success rate and cell survival rate are 100%and 84%,respectively.Compared to manual operation,this proposed system improves cell operation efficiency and cell survival rate.The proposed microinjection system has the potential to greatly reduce the workload of the experimenters and shorten the relevant study period.
