Objective: to explore the effect of micro-current stimulation on activation or inhibition of relevant signal pathways (MAPK, Wnt/β-catenin, NF-κB) in LDH model rats. Methods: a total of 180 healthy adult SD rats, ha...Objective: to explore the effect of micro-current stimulation on activation or inhibition of relevant signal pathways (MAPK, Wnt/β-catenin, NF-κB) in LDH model rats. Methods: a total of 180 healthy adult SD rats, half male and half female, weighing 250-280g, were selected for modeling. After successful modeling, NPCs cells of intervertebral disc were divided into stimulation group and non-stimulation group according to the intervention of low-frequency electronic pulse current stimulation. The contents of MAPK, Wnt/β-catenin, NF-κB signal pathway and LDH-related characteristic indicators in the two groups of rats were compared. Results: the relative expressions of p-p38MAPK, p-ERK1, p-ERK2 and p-JNK in the stimulation group were significantly lower than those in the non-stimulation group (p 0.05). The mechanical stimulation pain threshold of the stimulation group was significantly higher than that of the non-stimulation group, and the content of IL-1β and TNF-α was significantly lower than that of the non-stimulation group (P 0.05). There was no significant difference in IL-6 between the two groups. Conclusion: microcurrent stimulation can reduce the relative expression of p-p38MAPK, p-ERK1, p-ERK2, p-JNK protein in rat dorsal root ganglion, suggesting that it can inhibit ERK signaling pathway in rats with lumbar disc herniation.展开更多
文摘Objective: to explore the effect of micro-current stimulation on activation or inhibition of relevant signal pathways (MAPK, Wnt/β-catenin, NF-κB) in LDH model rats. Methods: a total of 180 healthy adult SD rats, half male and half female, weighing 250-280g, were selected for modeling. After successful modeling, NPCs cells of intervertebral disc were divided into stimulation group and non-stimulation group according to the intervention of low-frequency electronic pulse current stimulation. The contents of MAPK, Wnt/β-catenin, NF-κB signal pathway and LDH-related characteristic indicators in the two groups of rats were compared. Results: the relative expressions of p-p38MAPK, p-ERK1, p-ERK2 and p-JNK in the stimulation group were significantly lower than those in the non-stimulation group (p 0.05). The mechanical stimulation pain threshold of the stimulation group was significantly higher than that of the non-stimulation group, and the content of IL-1β and TNF-α was significantly lower than that of the non-stimulation group (P 0.05). There was no significant difference in IL-6 between the two groups. Conclusion: microcurrent stimulation can reduce the relative expression of p-p38MAPK, p-ERK1, p-ERK2, p-JNK protein in rat dorsal root ganglion, suggesting that it can inhibit ERK signaling pathway in rats with lumbar disc herniation.
