The variety of wound types has resulted in a wide range of wound dressings, with new products frequently being introduced to target different aspects of the wound healing process. The ideal wound dressing should achie...The variety of wound types has resulted in a wide range of wound dressings, with new products frequently being introduced to target different aspects of the wound healing process. The ideal wound dressing should achieve rapid healing at a reasonable cost, with minimal inconvenience to the patient. Microcurrent dressing, a novel wound dressing with inherent electric activity, can generate low-level microcurrents at the device-wound contact surface in the presence of moisture and can provide an advanced wound healing solution for managing wounds. This article offers a review of the effects and mechanisms of the microcurrent dressing on the healing of skin wounds.展开更多
Background:The exogenous application of low-intensity electric stimulation(ES)may mimic a natural endogenous bioelectric current and accelerate the repair process of skin wounds.This study designed a novel microcurren...Background:The exogenous application of low-intensity electric stimulation(ES)may mimic a natural endogenous bioelectric current and accelerate the repair process of skin wounds.This study designed a novel microcurrent dressing(MCD)and evaluated its potential effects on wound healing in a rat skin defect model.Methods:First,wireless ES was integrated into a medical cotton cushion to fabricate the MCD,and its electrical property was examined by using a universal power meter.Then,animal experiments were conducted to evaluate the MCD’s effect.Forty-five rats were randomized into control(Con)group,Vaseline gauze(VG)group and MCD group.A full-thickness round skin incision 1.5 cm in diameter was made on the back of each animal.Apart from routine disinfection,the Con rats were untreated,whereas the other two groups were treated with VG or MCD.On days 3,7 and 14 post injury,the wound areas were observed and measured using image analysis software following photography,and the skin samples were harvested from wound tissue.Then,histopathological morphology was observed routinely by hematoxylin and eosin(HE)staining;tumor necrosis factorα(TNF-α)and interleukin(IL)-1βexpression were detected by Western blotting.Vascular endothelial growth factor(VEGF)and epidermal growth factor(EGF)expression were detected with immunohistochemistry.Results:The MCD generated a sf electric potential greater than 0.95 V.Animal experiments showed that the woundhealing rate in the MCD group was significantly increased compared with the Con and VG groups(P<0.05 or P<0.01).Histopathological observation revealed an alleviated inflammatory response,induced vascular proliferation and accelerated epithelization in the MCD group.Moreover,samples from the MCD group expressed reduced TNF-αand IL-1βlevels and increased VEGF and EGF levels compared with those of the other two groups(P<0.05 or P<0.01).However,no significant difference was noted between the Con and VG groups at each time point.Conclusions:The MCD generates a stable and lasting ES and significantly promotes wound healing by reducing inflammation duration and increasing growth factors expression.Thus,MCD may act as a promising biomaterial device for skin wound healing.展开更多
The purpose of this study was to investigate the intensity-specific regenerative effects of microcurrent therapy on gastrocnemius muscle atrophy induced by cast-immobilization in rabbits. Fifteen rabbits were randomly...The purpose of this study was to investigate the intensity-specific regenerative effects of microcurrent therapy on gastrocnemius muscle atrophy induced by cast-immobilization in rabbits. Fifteen rabbits were randomly allocated to 3 groups after cast removal: cast-immobilization and sham microcurrent therapy for 2 weeks(group 1); castimmobilization and microcurrent therapy(25 μA) for 2 weeks(group 2); cast-immobilization and microcurrent therapy(5,000 μA) for 2 weeks(group 3). Clinical parameters [calf circumference, compound muscle action potential(CMAP) of the tibial nerve, thickness of gastrocnemius muscle], cross sectional area of gastrocnemius muscle fibres,and immunohistochemistry was evaluated. The clinical parameters representing mean atrophic changes in group 2 were significantly lower than those in group 3. The cross sectional area of the gastrocnemius muscle fibres and immunohistochemical parameters in group 2 were significantly greater than those in group 3. The results showed that low-intensity microcurrent therapy can more effectively promote regeneration in atrophied gastrocnemius muscle than high-intensity microcurrent therapy.展开更多
Objective: to explore the effect of micro-current stimulation on activation or inhibition of relevant signal pathways (MAPK, Wnt/β-catenin, NF-κB) in LDH model rats. Methods: a total of 180 healthy adult SD rats, ha...Objective: to explore the effect of micro-current stimulation on activation or inhibition of relevant signal pathways (MAPK, Wnt/β-catenin, NF-κB) in LDH model rats. Methods: a total of 180 healthy adult SD rats, half male and half female, weighing 250-280g, were selected for modeling. After successful modeling, NPCs cells of intervertebral disc were divided into stimulation group and non-stimulation group according to the intervention of low-frequency electronic pulse current stimulation. The contents of MAPK, Wnt/β-catenin, NF-κB signal pathway and LDH-related characteristic indicators in the two groups of rats were compared. Results: the relative expressions of p-p38MAPK, p-ERK1, p-ERK2 and p-JNK in the stimulation group were significantly lower than those in the non-stimulation group (p 0.05). The mechanical stimulation pain threshold of the stimulation group was significantly higher than that of the non-stimulation group, and the content of IL-1β and TNF-α was significantly lower than that of the non-stimulation group (P 0.05). There was no significant difference in IL-6 between the two groups. Conclusion: microcurrent stimulation can reduce the relative expression of p-p38MAPK, p-ERK1, p-ERK2, p-JNK protein in rat dorsal root ganglion, suggesting that it can inhibit ERK signaling pathway in rats with lumbar disc herniation.展开更多
Local microcurrent monitoring is of great significance for biological and battery systems,yet it poses a formidable challenge.The current measurement techniques rely on electromagnetic materials which inevitably intro...Local microcurrent monitoring is of great significance for biological and battery systems,yet it poses a formidable challenge.The current measurement techniques rely on electromagnetic materials which inevitably introduce interference to the system under examination.To address this issue,a promising approach based on a dielectric fiberoptic sensor is demonstrated.The microfiber is capable of detecting microcurrent through monitoring the localized proton concentration signal with a pH resolution of 0.0052 pH units.By sensing the refractive index variation surrounding the sensor induced by the interaction between local proton concentration changes and oxidizer-treated microfiber surface through the evanescent field,this sensing mechanism effectively avoids the interference of the electromagnetic material on the performance of the tested system.This sensor exhibits a limit of detection for microcurrent of 1μA.The sensing region is a microfiber with a diameter of 8.8μm.It can get invaluable information that cannot be obtained through conventional electrochemical methods.Examples include photocurrent attenuation in photogenerated carrier materials during illumination,electrical activation in nerve cells,and fluctuations in the efficiency of electrical energy generation during battery discharge.This approach provides a powerful complement to electrochemical methods for the elucidation of microscale reaction mechanisms.The information provided by the prepared dielectric fiber-optic sensor will shed more light on proton kinetics and electrochemical and electrobiological mechanisms,which may fll an important gap in the current bioelectricity and battery monitoring methods.展开更多
The efficacy requirements of cosmetic instruments and explored their efficacy evaluation methods was investigated.The efficacy and claims of common cosmetic instruments on the market were investigated,and human clinic...The efficacy requirements of cosmetic instruments and explored their efficacy evaluation methods was investigated.The efficacy and claims of common cosmetic instruments on the market were investigated,and human clinical trials were conducted to evaluate the efficacy of household cosmetic instruments.Household cosmetic instruments mainly include technologies such as sound wave,ion conduction,LED light,EMS microcurrent,RF radio frequency,and laser.This paper took microcurrent as an example to explore its efficacy claim methods.In human test I,the water content in the stratum corneum of the forearms of 31 subjects increased significantly compared with the blank control after 8 h.In human test II,30 subjects showed significant decreases in skin texture R5,transepidermal water loss TEWL,skin firmness F4 value,skin elasticity R2 value,overall size and depth of facial wrinkles,and facial jawline angle,and a significant increase in facial gloss value.The results showed that the cosmetic instrument had effects of moisturizing,improving facial evenness,repairing barrier,improving skin elasticity and firmness,and improving facial edema.展开更多
Assessment of muscle damage relies commonly on subjective sensation of pain. The purpose of this research was to test thevalidity of microcurrent conductance on skin over injured tissue to quantify soft tissue injury ...Assessment of muscle damage relies commonly on subjective sensation of pain. The purpose of this research was to test thevalidity of microcurrent conductance on skin over injured tissue to quantify soft tissue injury and recovery following heavy exercisecompared to other indexes of muscle soreness. A randomized, controlled, single-blinded, 1-week trial on 60 subjects.Setting-University Interventions： Subjects did 3 sets of squats for 5 min each. There were 3 groups of 20 subjects. One did nothing andone had heat applied for 8 h post exercise. The final group had heat 24 h after exercise. Tissue resistance and muscle strength force tomove the knee, analog visual pain scale. In the control group, microcurrent continually decreased, eventually decreasing 32% by thethird day post exercise. When heat was given immediately following exercise, microcurrent was 26% greater （P 〈 0.001）. The painscale rose to 3.1/10 as opposed to 5.4/10 for controls. Strength and muscle elasticity stayed mostly constant after heat as opposed to a28% decrease in strength and increase in stiffness in the control subjects. For 24 h delayed heat, microcurrent was 14% greater（P 〈 0.02）, and was unchanged for the first 24 h when no therapy was given. Pain scale rose to 4.8/10. Stiffness was unchanged whilemuscle strength decreased the same as controls. Microcurrent shows agreement with loss of strength, and stiffness from DOMS but notthe subjective pain measure. It appears that microcurrent is a good measure of muscle damage.展开更多
基金supported by the National Science Foundation of China(81301345)
文摘The variety of wound types has resulted in a wide range of wound dressings, with new products frequently being introduced to target different aspects of the wound healing process. The ideal wound dressing should achieve rapid healing at a reasonable cost, with minimal inconvenience to the patient. Microcurrent dressing, a novel wound dressing with inherent electric activity, can generate low-level microcurrents at the device-wound contact surface in the presence of moisture and can provide an advanced wound healing solution for managing wounds. This article offers a review of the effects and mechanisms of the microcurrent dressing on the healing of skin wounds.
基金supported by the National Natural Science Foundation of China(61402486)。
文摘Background:The exogenous application of low-intensity electric stimulation(ES)may mimic a natural endogenous bioelectric current and accelerate the repair process of skin wounds.This study designed a novel microcurrent dressing(MCD)and evaluated its potential effects on wound healing in a rat skin defect model.Methods:First,wireless ES was integrated into a medical cotton cushion to fabricate the MCD,and its electrical property was examined by using a universal power meter.Then,animal experiments were conducted to evaluate the MCD’s effect.Forty-five rats were randomized into control(Con)group,Vaseline gauze(VG)group and MCD group.A full-thickness round skin incision 1.5 cm in diameter was made on the back of each animal.Apart from routine disinfection,the Con rats were untreated,whereas the other two groups were treated with VG or MCD.On days 3,7 and 14 post injury,the wound areas were observed and measured using image analysis software following photography,and the skin samples were harvested from wound tissue.Then,histopathological morphology was observed routinely by hematoxylin and eosin(HE)staining;tumor necrosis factorα(TNF-α)and interleukin(IL)-1βexpression were detected by Western blotting.Vascular endothelial growth factor(VEGF)and epidermal growth factor(EGF)expression were detected with immunohistochemistry.Results:The MCD generated a sf electric potential greater than 0.95 V.Animal experiments showed that the woundhealing rate in the MCD group was significantly increased compared with the Con and VG groups(P<0.05 or P<0.01).Histopathological observation revealed an alleviated inflammatory response,induced vascular proliferation and accelerated epithelization in the MCD group.Moreover,samples from the MCD group expressed reduced TNF-αand IL-1βlevels and increased VEGF and EGF levels compared with those of the other two groups(P<0.05 or P<0.01).However,no significant difference was noted between the Con and VG groups at each time point.Conclusions:The MCD generates a stable and lasting ES and significantly promotes wound healing by reducing inflammation duration and increasing growth factors expression.Thus,MCD may act as a promising biomaterial device for skin wound healing.
基金supported by Basic Science Research Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Education (NRF-2016R1D1A1B01014260)
文摘The purpose of this study was to investigate the intensity-specific regenerative effects of microcurrent therapy on gastrocnemius muscle atrophy induced by cast-immobilization in rabbits. Fifteen rabbits were randomly allocated to 3 groups after cast removal: cast-immobilization and sham microcurrent therapy for 2 weeks(group 1); castimmobilization and microcurrent therapy(25 μA) for 2 weeks(group 2); cast-immobilization and microcurrent therapy(5,000 μA) for 2 weeks(group 3). Clinical parameters [calf circumference, compound muscle action potential(CMAP) of the tibial nerve, thickness of gastrocnemius muscle], cross sectional area of gastrocnemius muscle fibres,and immunohistochemistry was evaluated. The clinical parameters representing mean atrophic changes in group 2 were significantly lower than those in group 3. The cross sectional area of the gastrocnemius muscle fibres and immunohistochemical parameters in group 2 were significantly greater than those in group 3. The results showed that low-intensity microcurrent therapy can more effectively promote regeneration in atrophied gastrocnemius muscle than high-intensity microcurrent therapy.
文摘Objective: to explore the effect of micro-current stimulation on activation or inhibition of relevant signal pathways (MAPK, Wnt/β-catenin, NF-κB) in LDH model rats. Methods: a total of 180 healthy adult SD rats, half male and half female, weighing 250-280g, were selected for modeling. After successful modeling, NPCs cells of intervertebral disc were divided into stimulation group and non-stimulation group according to the intervention of low-frequency electronic pulse current stimulation. The contents of MAPK, Wnt/β-catenin, NF-κB signal pathway and LDH-related characteristic indicators in the two groups of rats were compared. Results: the relative expressions of p-p38MAPK, p-ERK1, p-ERK2 and p-JNK in the stimulation group were significantly lower than those in the non-stimulation group (p 0.05). The mechanical stimulation pain threshold of the stimulation group was significantly higher than that of the non-stimulation group, and the content of IL-1β and TNF-α was significantly lower than that of the non-stimulation group (P 0.05). There was no significant difference in IL-6 between the two groups. Conclusion: microcurrent stimulation can reduce the relative expression of p-p38MAPK, p-ERK1, p-ERK2, p-JNK protein in rat dorsal root ganglion, suggesting that it can inhibit ERK signaling pathway in rats with lumbar disc herniation.
基金was supported by the National Natural Science Foundation of China(No.62375108)the Guangdong Basic and Applied Basic Research Foundation(No.2024A1515010088)+1 种基金the Guangzhou Science and Technology Plan Project(No.2023A03J0130)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2019BT02X105).
文摘Local microcurrent monitoring is of great significance for biological and battery systems,yet it poses a formidable challenge.The current measurement techniques rely on electromagnetic materials which inevitably introduce interference to the system under examination.To address this issue,a promising approach based on a dielectric fiberoptic sensor is demonstrated.The microfiber is capable of detecting microcurrent through monitoring the localized proton concentration signal with a pH resolution of 0.0052 pH units.By sensing the refractive index variation surrounding the sensor induced by the interaction between local proton concentration changes and oxidizer-treated microfiber surface through the evanescent field,this sensing mechanism effectively avoids the interference of the electromagnetic material on the performance of the tested system.This sensor exhibits a limit of detection for microcurrent of 1μA.The sensing region is a microfiber with a diameter of 8.8μm.It can get invaluable information that cannot be obtained through conventional electrochemical methods.Examples include photocurrent attenuation in photogenerated carrier materials during illumination,electrical activation in nerve cells,and fluctuations in the efficiency of electrical energy generation during battery discharge.This approach provides a powerful complement to electrochemical methods for the elucidation of microscale reaction mechanisms.The information provided by the prepared dielectric fiber-optic sensor will shed more light on proton kinetics and electrochemical and electrobiological mechanisms,which may fll an important gap in the current bioelectricity and battery monitoring methods.
文摘The efficacy requirements of cosmetic instruments and explored their efficacy evaluation methods was investigated.The efficacy and claims of common cosmetic instruments on the market were investigated,and human clinical trials were conducted to evaluate the efficacy of household cosmetic instruments.Household cosmetic instruments mainly include technologies such as sound wave,ion conduction,LED light,EMS microcurrent,RF radio frequency,and laser.This paper took microcurrent as an example to explore its efficacy claim methods.In human test I,the water content in the stratum corneum of the forearms of 31 subjects increased significantly compared with the blank control after 8 h.In human test II,30 subjects showed significant decreases in skin texture R5,transepidermal water loss TEWL,skin firmness F4 value,skin elasticity R2 value,overall size and depth of facial wrinkles,and facial jawline angle,and a significant increase in facial gloss value.The results showed that the cosmetic instrument had effects of moisturizing,improving facial evenness,repairing barrier,improving skin elasticity and firmness,and improving facial edema.
文摘Assessment of muscle damage relies commonly on subjective sensation of pain. The purpose of this research was to test thevalidity of microcurrent conductance on skin over injured tissue to quantify soft tissue injury and recovery following heavy exercisecompared to other indexes of muscle soreness. A randomized, controlled, single-blinded, 1-week trial on 60 subjects.Setting-University Interventions： Subjects did 3 sets of squats for 5 min each. There were 3 groups of 20 subjects. One did nothing andone had heat applied for 8 h post exercise. The final group had heat 24 h after exercise. Tissue resistance and muscle strength force tomove the knee, analog visual pain scale. In the control group, microcurrent continually decreased, eventually decreasing 32% by thethird day post exercise. When heat was given immediately following exercise, microcurrent was 26% greater （P 〈 0.001）. The painscale rose to 3.1/10 as opposed to 5.4/10 for controls. Strength and muscle elasticity stayed mostly constant after heat as opposed to a28% decrease in strength and increase in stiffness in the control subjects. For 24 h delayed heat, microcurrent was 14% greater（P 〈 0.02）, and was unchanged for the first 24 h when no therapy was given. Pain scale rose to 4.8/10. Stiffness was unchanged whilemuscle strength decreased the same as controls. Microcurrent shows agreement with loss of strength, and stiffness from DOMS but notthe subjective pain measure. It appears that microcurrent is a good measure of muscle damage.
