The coronavirus disease 2019(COVID-19)has impacted health globally.Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders,diagnosed in patients even after the recove...The coronavirus disease 2019(COVID-19)has impacted health globally.Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders,diagnosed in patients even after the recovery period.In particular,micrometer-sized blood clots and hyperactivated platelets have been identified as potential indicators of long COVID.Here,we resolve microclot structures in the plasma of patients with different subphenotypes of COVID-19 in a label-free manner,using 3D digital holo-tomographic microscopy(DHTM).Based on 3D refractive index(RI)tomograms,the size,dry mass,and prevalence of microclot composites were quantified and then parametrically differentiated from fibrin-rich microclots and platelet aggregates in the plasma of COVID-19 patients.Importantly,fewer microclots and platelet aggregates were detected in the plasma of healthy controls compared to COVID-19 patients.Our imaging and analysis workflow is built around a commercially available DHT microscope capable of operation in clinical settings with a 2 h time period from sample preparation and data acquisition to results.展开更多
文摘The coronavirus disease 2019(COVID-19)has impacted health globally.Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders,diagnosed in patients even after the recovery period.In particular,micrometer-sized blood clots and hyperactivated platelets have been identified as potential indicators of long COVID.Here,we resolve microclot structures in the plasma of patients with different subphenotypes of COVID-19 in a label-free manner,using 3D digital holo-tomographic microscopy(DHTM).Based on 3D refractive index(RI)tomograms,the size,dry mass,and prevalence of microclot composites were quantified and then parametrically differentiated from fibrin-rich microclots and platelet aggregates in the plasma of COVID-19 patients.Importantly,fewer microclots and platelet aggregates were detected in the plasma of healthy controls compared to COVID-19 patients.Our imaging and analysis workflow is built around a commercially available DHT microscope capable of operation in clinical settings with a 2 h time period from sample preparation and data acquisition to results.
