Antimicrobial resistance(AMR)is a global public health issue.Rapid and accurate antimicrobial susceptibility tests(AST)on bacteria isolates would facilitate appropriate choice of antibiotics,in which patients receive ...Antimicrobial resistance(AMR)is a global public health issue.Rapid and accurate antimicrobial susceptibility tests(AST)on bacteria isolates would facilitate appropriate choice of antibiotics,in which patients receive appropriate treatment and the emergence of multidrug-resistant organisms could be prevented simultaneously.In this study,we have developed a microfluidic device named Self Dilution for Faster Antimicrobial Susceptibility Testing(SDFAST).This SlipChip-based device consists of two layers of microchips,allowing injection of bacterial suspension and antibiotics by simply connecting the two chips.By slipping one microchip against another in a single press of the microchip,the antibiotics can be diluted within seconds and be well mixed with bacterial samples.By combining SDFAST with a water-soluble tetrazolium salt-8(WST-8)assay,a range of clinically prevalent bacteria,including Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae,and Staphylococci species,were tested under various antibiotics.Color analysis after 4–6 h of incubation showed an abrupt change in the WST-8 color of certain wells with diluted antibiotics,proving that instrument-free and immediate identification of minimum inhibitory concentration(MIC)could be achieved.The testing on 51 clinical isolates had an agreement of 92%,proving the accuracy of our method.These results validated its advantages of simple operation,rapid testing,and low sample consumption comparing to conventional methods,which require 16–24 h of incubation.Therefore,our method shows great potential to be further developed into a medical instrument for automated medical testing and point-of-care diagnosis.展开更多
基金supported by the Health@InnoHK program of the Innovation and Technology Commission of the Hong Kong SAR Governmentsupported by The University of Hong Kong(202009185087)+1 种基金Collaborative Research Fund(C7165-20GF)General Research Fund(17307919 and 17303123)of the Research Grants Council of Hong Kong,Hong Kong.
文摘Antimicrobial resistance(AMR)is a global public health issue.Rapid and accurate antimicrobial susceptibility tests(AST)on bacteria isolates would facilitate appropriate choice of antibiotics,in which patients receive appropriate treatment and the emergence of multidrug-resistant organisms could be prevented simultaneously.In this study,we have developed a microfluidic device named Self Dilution for Faster Antimicrobial Susceptibility Testing(SDFAST).This SlipChip-based device consists of two layers of microchips,allowing injection of bacterial suspension and antibiotics by simply connecting the two chips.By slipping one microchip against another in a single press of the microchip,the antibiotics can be diluted within seconds and be well mixed with bacterial samples.By combining SDFAST with a water-soluble tetrazolium salt-8(WST-8)assay,a range of clinically prevalent bacteria,including Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae,and Staphylococci species,were tested under various antibiotics.Color analysis after 4–6 h of incubation showed an abrupt change in the WST-8 color of certain wells with diluted antibiotics,proving that instrument-free and immediate identification of minimum inhibitory concentration(MIC)could be achieved.The testing on 51 clinical isolates had an agreement of 92%,proving the accuracy of our method.These results validated its advantages of simple operation,rapid testing,and low sample consumption comparing to conventional methods,which require 16–24 h of incubation.Therefore,our method shows great potential to be further developed into a medical instrument for automated medical testing and point-of-care diagnosis.
