The inhibitory effect of methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours on some key enzymes[α-amylase,α-glucosidase and aldose reductase(AR)]linked to the pathology and complication...The inhibitory effect of methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours on some key enzymes[α-amylase,α-glucosidase and aldose reductase(AR)]linked to the pathology and complications of type 2 diabetes(T2D);and their antioxidant properties were evaluated.The antioxidant properties evaluated were DPPH•and ABTS•^+scavenging abilities,reducing power,and antioxidant phytochemicals(total phenolics,tannins,total flavonoids and total saponins).Extracts of both flours inhibitedα-amylase,α-glucosidase and AR in a dose-dependent manner.The half-maximal inhibitory concentrations(IC50)of B.eurycoma onα-amylase,α-glucosidase,AR and lipid peroxidation were lower than those of D.microcarpum,indicating that it had stronger inhibitory potency than D.microcarpum.B.eurycoma also had significantly(P<0.05)higher DPPH•and ABTS•^+scavenging abilities,and reducing power than D.microcarpum.The antioxidant phytochemicals(total phenolics,tannins,total flavonoids and total saponins)were also significantly(P<0.05)higher in B.eurycoma than D.microcarpum.The inhibitory effect of B.eurycoma and D.microcarpum extracts onα-amylase,α-glucosidase and AR activities may be attributed to the combined action of their polyphenols and total saponins,and this may be a possible mechanism of action providing support for their use in managing hyperglycemia and the complications of T2D.展开更多
Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic damage.Methods: Ethanol extract from D. microcarpum fruit pulp ...Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic damage.Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl,2,20-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide(CP) as genotoxic agent.Results: Ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical(generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been discussed.Conclusions: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment.展开更多
Coagulation-flocculation is a proven technique for the removal of suspended solids in wastewater, through the application of single angle turbidimetric measurement. Through this measurement, the coag-flocculation kine...Coagulation-flocculation is a proven technique for the removal of suspended solids in wastewater, through the application of single angle turbidimetric measurement. Through this measurement, the coag-flocculation kinetics and functional parameters behavior of Detarium microcarpumin brewery effluent with respect to pH, dosage and time were followed at room temperature. Process parameters such as order of reaction α, rate constant (K), coagulation period τ1/2 etc were determined. Results indicated that reaction order, rate constant, period, pH and dosage recorded optimum values at 2, 1.8 × 10-2 L/mg.min, 0.152 min, 4 and 100 mg/L, respectively. Maximum efficiency recorded was 96.07% at 30 min. Detarium microcarpum has shown potential as an effective bio-coagulant for the removal of turbidity from brewery effluent.展开更多
文摘The inhibitory effect of methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours on some key enzymes[α-amylase,α-glucosidase and aldose reductase(AR)]linked to the pathology and complications of type 2 diabetes(T2D);and their antioxidant properties were evaluated.The antioxidant properties evaluated were DPPH•and ABTS•^+scavenging abilities,reducing power,and antioxidant phytochemicals(total phenolics,tannins,total flavonoids and total saponins).Extracts of both flours inhibitedα-amylase,α-glucosidase and AR in a dose-dependent manner.The half-maximal inhibitory concentrations(IC50)of B.eurycoma onα-amylase,α-glucosidase,AR and lipid peroxidation were lower than those of D.microcarpum,indicating that it had stronger inhibitory potency than D.microcarpum.B.eurycoma also had significantly(P<0.05)higher DPPH•and ABTS•^+scavenging abilities,and reducing power than D.microcarpum.The antioxidant phytochemicals(total phenolics,tannins,total flavonoids and total saponins)were also significantly(P<0.05)higher in B.eurycoma than D.microcarpum.The inhibitory effect of B.eurycoma and D.microcarpum extracts onα-amylase,α-glucosidase and AR activities may be attributed to the combined action of their polyphenols and total saponins,and this may be a possible mechanism of action providing support for their use in managing hyperglycemia and the complications of T2D.
基金Supported by the International Foundation for Science(IFS)Stockholm under the IFS Grants F/5539-1 and F/4445-1+1 种基金the World Academy of Science(TWAS)Trieste under the grant TWAS 12-044 RG/BIO/AF/AC_G
文摘Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic damage.Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl,2,20-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide(CP) as genotoxic agent.Results: Ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical(generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been discussed.Conclusions: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment.
文摘Coagulation-flocculation is a proven technique for the removal of suspended solids in wastewater, through the application of single angle turbidimetric measurement. Through this measurement, the coag-flocculation kinetics and functional parameters behavior of Detarium microcarpumin brewery effluent with respect to pH, dosage and time were followed at room temperature. Process parameters such as order of reaction α, rate constant (K), coagulation period τ1/2 etc were determined. Results indicated that reaction order, rate constant, period, pH and dosage recorded optimum values at 2, 1.8 × 10-2 L/mg.min, 0.152 min, 4 and 100 mg/L, respectively. Maximum efficiency recorded was 96.07% at 30 min. Detarium microcarpum has shown potential as an effective bio-coagulant for the removal of turbidity from brewery effluent.
