Objective and Background Early and accurate diagnosis of spinal infections,including spinal tuberculosis,is pivotal for effective treatment but remains challenging.This study aims to assess the diagnostic yield of met...Objective and Background Early and accurate diagnosis of spinal infections,including spinal tuberculosis,is pivotal for effective treatment but remains challenging.This study aims to assess the diagnostic yield of metagenomic next-generation sequencing(mNGS)compared with that of conventional microbiological tests(CMTs)in identifying pathogens associated with spinal pathologies,with a special focus on infections leading to surgical interventions.Methods We enrolled 85 patients who underwent spinal surgery,comprising 63 patients with clinically diagnosed spinal infections,including patients with spinal tuberculosis,and 22 patients with noninfectious spinal conditions.The procedures involved irrigation and debridement for persistent wound drainage,with subsequent DNA extraction from plasma and joint fluid for mNGS and CMT analysis.Results Significantly increased C-reactive protein(CRP)levels were observed in patients with infections.The mNGS approach showed greater diagnostic sensitivity(92.06%)for detecting pathogens,including Mycobacterium tuberculosis,than did CMTs(36.51%).Despite its low specificity,mNGS had considerable negative predictive value(70.59%),underscoring its utility in ruling out infections.Conclusions The mNGS offers superior sensitivity over CMTs in the diagnosis of a variety of spinal infections,notably spinal tuberculosis.This study highlights the potential of mNGS in enhancing the diagnosis of complex spinal infections,thereby informing targeted treatment strategies.展开更多
Objective:To observe and analyze the effect of bacterial resistance monitoring in clinical microbiology testing.Methods:600 microbial specimens collected in our hospital in the past year(April 2021 to April 2022)were ...Objective:To observe and analyze the effect of bacterial resistance monitoring in clinical microbiology testing.Methods:600 microbial specimens collected in our hospital in the past year(April 2021 to April 2022)were used as the test subjects of this study.The specimens were divided into Group A(control group)and Group B(research group),with 300 cases in each group.Group A consisted of blood culture specimens,while Group B consisted of sputum specimens.After the tests were completed,the rates of unfavorable and favorable results,bacterial species distribution,and bacterial drug resistance of the specimens in both groups were compared.Results:Among group A specimens,29 cases were positive(9.67%)and 271 cases were negative(90.33%);among group B specimens,99 cases were positive(33.00%)and 201 cases were negative(66.00%);the difference between the two groups of data was statistically significant(P<0.05).As for the distribution of the types of bacteria,there were 472 cases of Gram-negative bacteria and 128 cases of Gram-positive bacteria.Conclusion:Bacterial resistance monitoring is helpful in clinical microbiology testing.Through proper monitoring,bacterial resistance can be well understood.In this way,patients get to receive appropriate treatment measures and suitable antibacterial prescriptions,thereby improving the patient outcome.展开更多
At present, microbiological examination has become an indispensable part of diagnosis and treatment, which provides high-quality reference for modern medical treatment. It can improve the quality of test results and d...At present, microbiological examination has become an indispensable part of diagnosis and treatment, which provides high-quality reference for modern medical treatment. It can improve the quality of test results and develop individualized treatment plans according to the specific conditions of patients. Based on this, the author carried out the following analysis, starting from the basic overview of microbiological inspection, so as to explore the key points of microbiological inspection quality control, and put forward specific control strategies, to provide reference suggestions for the majority of practitioners and inspectors.展开更多
Objective: in the process of testing Candida vaginitis patients to take different microbiological test methods, mainly to observe the effect of vaginal secretion test.Methods: a total of 84 patients diagnosed with can...Objective: in the process of testing Candida vaginitis patients to take different microbiological test methods, mainly to observe the effect of vaginal secretion test.Methods: a total of 84 patients diagnosed with candida vaginitis were enrolled in this study. The vaginal secretions of the patients were examined by microscopy, culture and agglutination, and the results were compared.Results: compared with culture method and agglutination method, the positive detection rate of microscopy method was higher, the difference was significant (P < 0.05).Conclusion: microscopic examination can achieve good results in the examination of vaginal secretions in patients with candida vaginitis, and it can improve the detection rate of candida. This method is worthy of popularization and application.展开更多
基金supported by the Hubei Provincial Natural Science Foundation of China(No.2023AFB646)Knowledge Innovation Program of Wuhan(No.2023020201010155)Educational Research Program of Huazhong University of Science and Technology(No.2022135).
文摘Objective and Background Early and accurate diagnosis of spinal infections,including spinal tuberculosis,is pivotal for effective treatment but remains challenging.This study aims to assess the diagnostic yield of metagenomic next-generation sequencing(mNGS)compared with that of conventional microbiological tests(CMTs)in identifying pathogens associated with spinal pathologies,with a special focus on infections leading to surgical interventions.Methods We enrolled 85 patients who underwent spinal surgery,comprising 63 patients with clinically diagnosed spinal infections,including patients with spinal tuberculosis,and 22 patients with noninfectious spinal conditions.The procedures involved irrigation and debridement for persistent wound drainage,with subsequent DNA extraction from plasma and joint fluid for mNGS and CMT analysis.Results Significantly increased C-reactive protein(CRP)levels were observed in patients with infections.The mNGS approach showed greater diagnostic sensitivity(92.06%)for detecting pathogens,including Mycobacterium tuberculosis,than did CMTs(36.51%).Despite its low specificity,mNGS had considerable negative predictive value(70.59%),underscoring its utility in ruling out infections.Conclusions The mNGS offers superior sensitivity over CMTs in the diagnosis of a variety of spinal infections,notably spinal tuberculosis.This study highlights the potential of mNGS in enhancing the diagnosis of complex spinal infections,thereby informing targeted treatment strategies.
文摘Objective:To observe and analyze the effect of bacterial resistance monitoring in clinical microbiology testing.Methods:600 microbial specimens collected in our hospital in the past year(April 2021 to April 2022)were used as the test subjects of this study.The specimens were divided into Group A(control group)and Group B(research group),with 300 cases in each group.Group A consisted of blood culture specimens,while Group B consisted of sputum specimens.After the tests were completed,the rates of unfavorable and favorable results,bacterial species distribution,and bacterial drug resistance of the specimens in both groups were compared.Results:Among group A specimens,29 cases were positive(9.67%)and 271 cases were negative(90.33%);among group B specimens,99 cases were positive(33.00%)and 201 cases were negative(66.00%);the difference between the two groups of data was statistically significant(P<0.05).As for the distribution of the types of bacteria,there were 472 cases of Gram-negative bacteria and 128 cases of Gram-positive bacteria.Conclusion:Bacterial resistance monitoring is helpful in clinical microbiology testing.Through proper monitoring,bacterial resistance can be well understood.In this way,patients get to receive appropriate treatment measures and suitable antibacterial prescriptions,thereby improving the patient outcome.
文摘At present, microbiological examination has become an indispensable part of diagnosis and treatment, which provides high-quality reference for modern medical treatment. It can improve the quality of test results and develop individualized treatment plans according to the specific conditions of patients. Based on this, the author carried out the following analysis, starting from the basic overview of microbiological inspection, so as to explore the key points of microbiological inspection quality control, and put forward specific control strategies, to provide reference suggestions for the majority of practitioners and inspectors.
文摘Objective: in the process of testing Candida vaginitis patients to take different microbiological test methods, mainly to observe the effect of vaginal secretion test.Methods: a total of 84 patients diagnosed with candida vaginitis were enrolled in this study. The vaginal secretions of the patients were examined by microscopy, culture and agglutination, and the results were compared.Results: compared with culture method and agglutination method, the positive detection rate of microscopy method was higher, the difference was significant (P < 0.05).Conclusion: microscopic examination can achieve good results in the examination of vaginal secretions in patients with candida vaginitis, and it can improve the detection rate of candida. This method is worthy of popularization and application.
