Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect their gut microbiota and resistome. T...Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect their gut microbiota and resistome. To better understand how continuous antimicrobial use in farm animals alters their microbial ecology, we used a metagenomic approach to investigate the effects of pulsed antimicrobial administration on the bacterial community, antibiotic resistance genes(ARGs) and ARG bacterial hosts in the feces of broiler chickens. Chickens received three 5-day courses of individual or combined antimicrobials, including amoxicillin, chlortetracycline and florfenicol. The florfenicol administration significantly increased the abundance of mcr-1 gene accompanied by floR gene, while amoxicillin significantly increased the abundance of genes encoding the AcrAB-tolC multidrug efflux pump(marA, soxS, sdiA, rob, evgS and phoP).These three antimicrobials all led to an increase in Proteobacteria. The increase in ARG host, Escherichia, was mainly attributed to the β-lactam, chloramphenicol and tetracycline resistance genes harbored by Escherichia under the pulsed antimicrobial treatments. These results indicated that pulsed antimicrobial administration with amoxicillin,chlortetracycline, florfenicol or their combinations significantly increased the abundance of Proteobacteria and enhanced the abundance of particular ARGs. The ARG types were occupied by the multidrug resistance genes and had significant correlations with the total ARGs in the antimicrobial-treated groups. The results of this study provide comprehensive insight into pulsed antimicrobial-mediated alteration of chicken fecal microbiota and resistome.展开更多
Direct-fed microbials(DFM) are considered as a promising technique to improve animal productivity without affecting animal health or harming the environment.The potential of three bacterial DFM to reduce methane(CH4)e...Direct-fed microbials(DFM) are considered as a promising technique to improve animal productivity without affecting animal health or harming the environment.The potential of three bacterial DFM to reduce methane(CH4)emissions,modulate ruminal fermentation,milk production and composition of primiparous dairy cows was examined in this study.As previous reports have shown that DFM respond differently to different diets,two contrasting diets were used in this study.Eight lactating primiparous cows were randomly divided into two groups that were fed a corn silage-based,high-starch diet(HSD) or a grass silage-based,high-fiber diet(HFD).Cows in each dietary group were randomly assigned to four treatments in a 4 × 4 Latin square design.The bacterial DFM used were selected for their proven CH4-reducing effect in vitro.Treatments included control(without DFM) and 3 DFM treatments: Propionibacterium freudenreichii 53-W(2.9 × 10^10 colony forming units(CFU)/cow per day),Lactobacillus pentosus D31(3.6 × 10^11 CFU/cow per day) and Lactobacillus bulgaricus D1(4.6 × 10^10 CFU/cow per day).Each experimental period included 4 weeks of treatment and 1 week of wash-out,with measures performed in the fourth week of the treatment period.Enteric CH4 emissions were measured during 3 consecutive days using respiration chambers.Rumen samples were collected for ruminal fermentation parameters and quantitative microbial analyses.Milk samples were collected for composition analysis.Body weight of cows were recorded at the end of each treatment period.Irrespective of diet,no mitigating effect of DFM was observed on CH4 emissions in dairy cows.In contrast,Propionibacterium increased CH4 intensity by 27%(g CH4/kg milk) in cows fed HSD.There was no effect of DFM on other fermentation parameters and on bacterial,archaeal and protozoal numbers.Similarly,the effect of DFM on milk fatty acid composition was negligible.Propionibacterium and L.pentosus DFM tended to increase body weight gain with HSD.We conclude that,contrary to the effect previously observed in vitro,bacterial DFM Propionibacterium freudenreichii 53-W,Lactobacillus pentosus D31 and Lactobacillus bulgaricus D1 did not alter ruminal fermentation and failed to reduce CH4 emissions in lactating primiparous cows fed high-starch or high-fiber diets.展开更多
BACKGROUND:Breast hyperplasia is a common benign breast disease mainly caused by endocrine disorders,manifested as abnormal hyperplasia of breast tissue.In recent years,traditional Chinese medicine compounds and probi...BACKGROUND:Breast hyperplasia is a common benign breast disease mainly caused by endocrine disorders,manifested as abnormal hyperplasia of breast tissue.In recent years,traditional Chinese medicine compounds and probiotics have shown good potential in regulating the endocrine system and improving the intestinal microecology,providing new ideas for the treatment of breast hyperplasia.OBJECTIVE:To explore the effects and mechanisms of traditional Chinese medicine compounds and fermented probiotic compounds on breast hyperplasia in mice,providing new theoretical and experimental bases for the clinical treatment and prevention of breast hyperplasia.METHODS:(1)Network pharmacology tools were used to predict the anti-breast-hyperplasia activity of Herba Gueldenstaedtiae(Euphorbia humifusa),as well as its potential targets and signaling pathways.The databases included:TCMSP,OMIM,GeneCards database,UniProt website,Venny2.1.0 website,Metascape,HERB website,and STRING database,all of which are open-access databases.Network pharmacology can predict and screen key information such as the targets corresponding to the active ingredients of traditional Chinese medicine,disease targets,and action pathways through network analysis and computer-system analysis.Therefore,it has been increasingly widely used in the research of traditional Chinese medicine.(2)A breast hyperplasia model was induced in mice by injecting estrogen and progesterone.Mice in the normal blank group were injected intraperitoneally with normal saline every day.Mice in the model group and drugadministration groups were injected intraperitoneally with estradiol benzoate injection at a concentration of 0.5 mg/kg every day for 25 days.From the 26th day,the injection of estradiol benzoate injection was stopped.Mice in the normal blank group were injected intramuscularly with normal saline every day,and mice in the model group and drug-administration groups were injected intramuscularly with progesterone injection at a concentration of 5 mg/kg for 5 days.After the model was established,each group was given drugs respectively.The normal blank group and the model group were gavaged with 0.2 mL/d of normal saline;the positive blank group(Xiaozheng Pill group)was gavaged with an aqueous solution of Xiaozheng Pill at 0.9 mg/g;the low-,medium-and high-dose groups of Compound Herba Gueldenstaedtiae were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively;the low-,medium-and high-dose groups of traditional Chinese medicine-bacteria fermentation were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively.The administration was continuous for 30 days.RESULTS AND CONCLUSION:(1)The results of network pharmacology research showed that the Compound Herba Gueldenstaedtiae(Euphorbia humifusa)contained 46 active ingredients,which were related to 1213 potential targets.After comparison with 588 known breast-hyperplasia targets,it was speculated that 50 of these targets might be related to the direct effect of the compound on breast hyperplasia.(2)After drug intervention,there was no significant change in the high-dose group of Compound Herba Gueldenstaedtiae compared with the normal blank group.The liver indicators of the other intervention groups all significantly decreased(P<0.05).(3)In terms of kidney and uterine indicators,the medium-dose group of Compound Herba Gueldenstaedtiae decreased significantly compared with the normal blank group(P<0.05).In terms of the uterine index,the model group increased significantly compared with the normal blank group(P<0.01).(4)After 1-month drug treatment,the number of lobules and acini in the breast tissue of the Xiaozheng Pill group,the low,medium,and high-dose group of Compound Herba Gueldenstaedtiae,the low,medium,and highdose groups of traditional Chinese medicine-bacteria fermentation decreased,and the duct openings narrowed.With the increase of drug dose,diffuse hyperplasia of breast tissue was significantly improved.(5)The ELISA results showed that compared with the model group,the estrogen level was lower in the medium-dose group of traditional Chinese medicine-bacteria fermentation after the intervention(P<0.05).In addition,the follicle-stimulating hormone level in the low-dose group of Compound Herba Gueldenstaedtiae was lower than that of the model group(P<0.05).(6)The intervention in the mouse model led to changes in the abundance of short chain fatty acids and intestinal flora in all groups.To conclude,the Compound Herba Gueldenstaedtiae and its probiotic fermentation products significantly improved mammary gland hyperplasia in mice by regulating hormone levels,improving the structure of the gut microbiota,and increasing the content of shortchain fatty acids,providing new ideas and potential sources of drugs for the treatment of breast hyperplasia.展开更多
The gut microbiota:The human body is colonized by a diverse and complex microbial community–including bacteria,viruses,archaea,and unicellular eukaryotes–that plays a central role in human wellbeing.Indeed,microbiot...The gut microbiota:The human body is colonized by a diverse and complex microbial community–including bacteria,viruses,archaea,and unicellular eukaryotes–that plays a central role in human wellbeing.Indeed,microbiota is crucial for several functions,including host metabolism,physiology,maintenance of the intestinal epithelial integrity,nutrition,and immune function,earning it the designation of a“vital organ”(Guinane and Cotter,2013).展开更多
Rapidly improving infertile croplands and enhancing their soil organic carbon(SOC)pool necessitate substantial organic materials incorporation.Converting loose crop straw into granulated form facilitates uniform incor...Rapidly improving infertile croplands and enhancing their soil organic carbon(SOC)pool necessitate substantial organic materials incorporation.Converting loose crop straw into granulated form facilitates uniform incorporation within the plough soil layer.As an innovative soil amelioration approach,the efficiency and patterns of SOC accumulation remain unclear.Two field experiments were conducted in infertile subtropical upland and paddy soils with 0,30,60,and 90 Mg ha^(-1)granulated straw incorporation.After one year,SOC accumulation efficiency from straw input remained stable in upland(30.8–37.5%)with increasing amounts of straw incorporation,while declined from 60.0 to 38.3%in paddy.In both croplands,the contributions of lignin phenols to SOC increased with increasing straw incorporation,while the contributions from amino sugars remained constant at higher straw input levels.Subsequently,the ratios of lignin phenols to amino sugars increased with increasing straw incorporation,indicating faster plant residue accumulation compared to microbial necromass,as the granulation approach limited microbial involvement in straw transformation.Thus,single-time incorporation of substantial granulated straw presents an effective agricultural strategy for rapid amelioration of infertile croplands.展开更多
The Earth's environment is undergoing significant transformation due to mining,pollution,and climate change.Although mining is essential for economic development,it contributes significantly to the release of pote...The Earth's environment is undergoing significant transformation due to mining,pollution,and climate change.Although mining is essential for economic development,it contributes significantly to the release of potentially harmful elements(PHEs)that threaten human health and destabilize microbial communities.Anthropogenic climate change,driven by greenhouse gas emissions,alters water availability and soil composition,further affecting ecosystem balance and microbial diversity.This review aggregates findings from studies covering the last two decades to assess how mining pollution and climate change impact microbial diversity,their adaptation mechanisms,and the associated health risks.It reveals that environmental stressors favour resistant microbial taxa while eliminating sensitive species,thereby reshaping microbial ecosystems.Microorganisms demonstrate genetic,biochemical,and physiological adaptations that enable them to survive in polluted or changing environments,often resulting in a higher prevalence of pathogenic and antibiotic-resistant strains.These variations in microbial dynamics contribute to health challenges such as respiratory infections,foodborne illnesses,and increased exposure in children and immune-compromised individuals.The review highlights the linkages between environmental degradation,microbial ecology,and human health,underscoring the need for integrative policies and interventions to mitigate long-term risks,support microbial ecosystem stability,promote sustainable health and agricultural outcomes.展开更多
Suancai has a lengthy history and a wide range of categories,which has some influence on the pickled diet culture around the world.Suancai production is transitioning to a large-scale,standardized production due to th...Suancai has a lengthy history and a wide range of categories,which has some influence on the pickled diet culture around the world.Suancai production is transitioning to a large-scale,standardized production due to the growth of the market.It has a unique flavor and is rich in nutrients,and its abundance of free amino acids,vitamins and phenolics has many positive effects on the human body.This review gives the types and history of suancai,as well as its impact on the world’s pickled culture.The changes in nutritional composition and flavor of suancai during fermentation are summarized.It presented the production technology and influencing factors of the northeast suancai,examined the quality and safety issues in suancai,and put forth some ideas and opinions on the standardization development of the suancai industry.It also summarized the geographic distribution and flora diversity of pickles around the world.In order to provide some knowledge and guidance for the promotion of modern industrial production in the suancai industry.展开更多
Pyrethroids are a class of novel broad-spectrum pesticides synthesized to mimic natural pyrethrins.Due to their high efficiency,low toxicity,and safety,pyrethroids have been widely used as alternatives to organophosph...Pyrethroids are a class of novel broad-spectrum pesticides synthesized to mimic natural pyrethrins.Due to their high efficiency,low toxicity,and safety,pyrethroids have been widely used as alternatives to organophosphate and carbamate insecticides in the control of agricultural and sanitary pests.However,with the increasing use of pyrethroid pesticides,the resulting pesticide residues have posed threats to both the environment and human health.Biodegradation is considered one of the most promising methods for the removal of pyrethroids,and significant research has been conducted in this area.This review summarizes recent advances in the biodegradation of pyrethroids,including degradation by single strains,microbial consortia,and enzymes.It provides an in-depth analysis of the biodegradation pathways and catalytic mechanisms involved in the degradation of pyrethroids and outlines enhancement strategies for improving the activity of pyrethroid-degrading enzymes.The review also identifies current challenges in pyrethroid biodegradation and offers perspectives for future research.This review serves as a valuable reference for subsequent studies on pyrethroid biodegradation.展开更多
Microplastic accumulation after film mulching affects nutrients cycling in the soil–crop system.Bulk soil(BS)and rhizosphere soil(RS)have two different community compositions which lead to their different microbial n...Microplastic accumulation after film mulching affects nutrients cycling in the soil–crop system.Bulk soil(BS)and rhizosphere soil(RS)have two different community compositions which lead to their different microbial nutrient acquisition abilities.Microplastics influence the rhizosphere effect.However,the mechanism by which microplastic accumulation affects the net photosynthetic rate(NPR)through rhizospheric microbial communities remains unknown.This study aimed to identify the mechanisms underlying the effects of polyethylene(PE)and polyvinyl chloride(PVC)microplastics at 0,1,and 5%(w/w)on the NPR in the wheat–soil ecosystem using a pot experiment.Superoxide dismutase(SOD)activity was reduced by 15.35–36.7%,and that of peroxidase(POD)was increased by 32.47–61.93%,causing reductions in NPR(17.94–23.81%)in the PE5%and PVC(1 and 5%)(w/w)treatments compared with the control.The Chao1,Shannon,and Simpson indices of the bacterial and fungal diversities were lower in BS than in RS at PE1%and PVC5%(w/w),respectively.The bacterial and fungal network complexities were reduced and increased,respectively,owing to alterations in the bacterial and fungal community compositions and structures for wheat growth.The Mantel test showed that the bacterial and fungal diversity indices in BS had positive correlations with Olsen-P and phosphatase;however,those in RS were positively correlated with NO_(3)^(–) and β-1,4-glucosidase.The structural equation model indicated that wheat enzymatic and soil hydrolytic activities negatively affected NPR.Wheat has a profound antioxidant defense strategy for PE and PVC microplastic stress,which produces a synergistic effect of POD by protecting organelles and reducing tissue damage to preserve the NPR.展开更多
To explore the adaptive mechanisms of the partial nitritation-anammox(PNA)process under high salinity stress during kitchen wastewater treatment,focusing on their physiological and molecular responses through metageno...To explore the adaptive mechanisms of the partial nitritation-anammox(PNA)process under high salinity stress during kitchen wastewater treatment,focusing on their physiological and molecular responses through metagenomic analysis.An airlift inner-circulation partition bioreactor(AIPBR)was developed,featuring an inner cylinder and a flow guide tube to create distinct oxygen gradients,facilitating the study of microbial adaptation under varying salt conditions.The AIPBR was operated with synthetic wastewater containing ammonium concentrations of 1800±100 mg/L and salinity gradients ranging from 1 to 10 g/L,followed by a fixed salinity period at 6 g/L,with ammonium concentrations approximately 850 mg/L.High-throughput metagenomic analysis revealed shifts in functional genes and metabolic pathways in response to salinity stress.Anammox bacteria adapted by enriching genes involved in the synthesis of osmoprotective compounds and activating energy-producing pathways like the tricarboxylic acid cycle(TCA).These adaptations,along with modifications in membrane composition,were essential for sustaining system stability under elevated salinity.Under prolonged high salinity stress,anaerobic ammonium oxidizing(AnAOB)exhibited improved salt tolerance,maintaining a total nitrogen removal efficiency above 85%and stabilizing after an adaptation phase.The metagenomic data revealed a marked enrichment of genes associated with ion transport,stress response mechanisms,and DNA repair pathways.Changes in microbial community composition favored salt-tolerant species,supporting system stability.These findings highlight the applicability of the developed bioreactor for scaling up the PNA process to handle high-salinity wastewater,providing a promising avenue for sustainable nitrogen removal in challenging environments.展开更多
Soil water content and salinity critically regulate soil microbial composition,plant community structure,and ecosystem multifunctionality(EMF)in semi-arid grasslands.However,the mechanisms through which drought(D),sal...Soil water content and salinity critically regulate soil microbial composition,plant community structure,and ecosystem multifunctionality(EMF)in semi-arid grasslands.However,the mechanisms through which drought(D),saline-alkaline(SA),and their combined(DSA)stress influence these ecological components remain poorly understood.This study investigated these mechanisms along natural gradients in a semi-arid grassland of China by analyzing soil physical-chemical properties,microbial communities,and vegetation characteristics.The results showed that as the environmental stress shifted from the D group to the DSA group and then to the SA group,soil electrical conductivity significantly increased,while urease and phosphatase activities significantly decreased.Soil organic carbon,total nitrogen,total phosphorus,and microbial biomass carbon and nitrogen were lower in the D and SA groups than in the DSA group.Meanwhile,plant biomass showed an increasing trend along the treatment gradient,primarily driven by dominant species,while plant diversity did not exhibit significant differences.Further analysis identified the soil water content and salinity as the key determinants of soil microbial diversity and community complexity.Soil enzyme activities exhibited contrasting relationships with microbial composition,correlating positively with the richness of bacterial amplicon sequence variants(ASVs)but negatively with the richness of fungal ASVs.Notably,microbial biomass,which varied significantly across different groups,emerged as a key predictor of changes in EMF,with its critical role confirmed through structural equation modeling.These findings collectively elucidate the responses of ecological communities to synergistic soil hydro-saline stress in semi-arid ecosystems,while highlighting the critical role of microbial biomass in maintaining EMF.展开更多
The remediation of arsenic(As)-contaminated soil is essential for achieving sustainable environmental and agricultural development.Among various techniques,soil washing has emerged as a promising method due to its rap...The remediation of arsenic(As)-contaminated soil is essential for achieving sustainable environmental and agricultural development.Among various techniques,soil washing has emerged as a promising method due to its rapid,efficient,and thorough decontamination capabilities.This review critically examines the application of soil washing technology in the treatment of As-contaminated soil.Specifically,this paper discusses the mechanisms of four types of washing agents(inorganic detergents,chelating agents,surfactants,and microbial agents),focusing on processes such as acid dissolution,electrostatic interaction,ion exchange,and chelation,and the factors affecting washing efficiency.The concentration of washing agent and the initial p H are the key factors influencing the washing effect.The paper also summarizes the application conditions and the corresponding removal rates for different individual washing agents and compares their effectiveness,biodegradability,and environmental impacts.Among these,natural chelating agents are highlighted for their promising potential in As removal.While individual washing agents show certain effectiveness,the combined use of multiple washing agents and the optimization of washing sequence are necessary to achieve superior remediation outcomes.The synergistic effects of combining natural chelating agents with reducing agents,surfactants,and inorganic washing agents,as well as the integration of nanomaterials with chelating agents and microbial agents are summarized,demonstrating their efficiency and stability in soil remediation.By reviewing the current state of research,this paper provides essential insights for the selection of washing agents and the optimization of washing parameters in the remediation of As-contaminated soil.展开更多
Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen micro...Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied.This study investigated changes in rumen bacteria,archaea,and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.Results A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation.The ST study(95 samples)used eight ruminally cannulated beef cattle in a 4×4 Latin square design with four 28-d of 3-NOP treatments[mg/kg of dry matter(DM)]:control:0,low:53,med:161,and high:345.The LT study(48 samples)was a completely randomized design with two 3-NOP treatments[control:0,and high:280 mg/kg of DM)fed for 112-d followed by a 16-d withdrawal(without 3-NOP).Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed.Under ST supplementation,the relative abundances of Prevotella,Methanobrevibacter(Mbb.)ruminantium,Methanosphaera sp.ISO3-F5,and Entodinium were increased(Q<0.05),whereas those of Mbb.gottschalkii and Epidinium were decreased(Q<0.05)with 3-NOP supplementation.In LT study,relative abundances of Mbb.ruminantium,and Methanosphaera sp.Group5 were increased(Q<0.05),while those of Saccharofermentans and Mbb.gottschalkii were decreased(Q<0.05)with 3-NOP supplementation.Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp.Group5(P<0.05)after 3-NOP withdrawal.Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher(P=0.029)relative abundances of Eggerthellaceae DNF00809,p-1088-a5 gut group,and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa.Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.Conclusions Both ST and LT supplementation affected overall rumen microbial profile,with individual microbial groups responded to 3-NOP supplementation differently.After 3-NOP withdrawal,not all microbes showed recovery,indicating that the 3-NOP driven shifts were only partially reversible.These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.展开更多
Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon&...Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon'grapes sourced from organic and conventional management vineyard(OMV/CMV)in the Ningxia region were subjected to spontaneous fermentation.The microbial,oenological,and aroma profiles of grape must and resulting wines were assessed using high-throughput sequencing(HTS),high-performance liquid chromatography(HPLC),gas chromatography with mass spectrometry(GC-MS),and sensory evaluations.Network analysis was applied to explore relationships among microorganisms,volatile compounds,and aroma attributes.Results showed that organic management significantly increased microbial species richness,α-diversity,and the variety and concentration of aroma compounds,favoring the production of natural wines with complex aroma profiles.Relative abundance of Saccharomyces in OMV reduced,promoting the prevalence of other yeast species during fermentation.Bacterial succession in wines from OMV remained stable,with Pantoea as the dominant genus.Among oenological parameters,OMV wines significantly induced glycerol content,while reduced total acidity,tartaric acid,and citric acid content.These wines exhibited significantly higher levels of fermentative(+16%)and varietal(+72%)volatiles,as well as enhanced floral and sweet fruity aromas,along with distinct nail polish and vegetal notes.Additionally,Saccharomyces,Hanseniaspora,Metschnikowia,and Pantoea were strongly correlated with specific volatile compounds and aroma characteristics.This study provides valuable data that can inform spontaneous fermentation practices and guide vineyard management for natural wine production.展开更多
This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from...This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from the Dahei River prior to DNA extraction and 16S rRNA gene sequencing,it generates standard curves to convert sequencing data into absolute microbial copy numbers.The method,which is proved highly accurate(R^(2)>0.99),reveals a clear contrast between the river sites:the upstream community has not only a significantly higher total microbial load but also a completely different makeup of species compared to the downstream site.This approach effectively overcomes the limitations of relative abundance analysis,providing a powerful tool for environmental monitoring,and proposes key steps for future standardization to ensure data comparability and integration.展开更多
The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litope...The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litopenaeus vannamei as a model species and two strains of Bacillus(W1 and XYB4)combined with sodium humate under zero-water exchange conditions.The growth performance,enzyme activity,and aquatic system microbial environment of L.vannamei were analyzed.Results showed that the combination of sodium humate and Bacillus strains effectively enhanced environmental conditions for the growth and reproduction of heterotrophic bacteria while inhibiting the growth of Vibrio species,including green and yellow variants.Microbiome analysis showed that the group treated with Bacillus strains combined with sodium humate exhibited significantly higher relative abundances of Firmicutes and Actinobacteriota than the other groups.Correspondingly,this treatment group showed substantially enhanced weight gain rate,specific growth rate,survival rate,and feed coefficient.Moreover,the phenol oxidase,catalase,lysozyme,and superoxide dismutase indexes of shrimps subjected to Bacillus–sodium humate treatment were considerably higher than those of the control group.These findings confirm that the combination of Bacillus and sodium humate has beneficial effects on shrimp growth and aquatic system quality control,providing a promising strategy for enhancing the efficiency of shrimp farming and aquaculture.展开更多
The microbial degradation of aromatic organic pollutants is incomplete due to their metabolic characteristics,which can easily produce certain highly toxic intermediates.Therefore,this article designs a dual template ...The microbial degradation of aromatic organic pollutants is incomplete due to their metabolic characteristics,which can easily produce certain highly toxic intermediates.Therefore,this article designs a dual template molec-ularly imprinted sensor(DTMIP/Fe-Mn@C)for iron manganese metal nanomaterials,prepared Fe-Mn@C com-posite materials by a one pot method were coated on the surface of glassy carbon electrodes and covered with molecularly imprinted membranes through electropolymerization and elution methods,achieving real-time de-tection of specific intermediate products 2-methylbutyric acid(2-MBA)and 3-methylbutyric acid(3-MBA)de-graded by azo dyes.In order to determine the detection sensitivity and intensity range of the sensor,optimization experiments were conducted on various parameters that affect the detection performance,such as the type of func-tional monomer and its composition ratio with the template molecule,detection time window,environmental pH value,etc.Finally,o-Phenylenediamine was determined as the functional monomer,with a molar ratio of 1:1:6 to the template molecules 2-MBA and 3-MBA.Electrochemical testing was conducted in a neutral environment with an incubation time of 5 min and pH=7.The results indicate that the sensor has a relatively wide detection range,high sensitivity,obvious recognition features,and excellent stability for 2-MBA and 3-MBA.This new dual template molecularly imprinted sensor can quickly and accurately determine the safety of highly toxic interme-diates in the degradation process of aromatic organic pollutants,providing a theoretical basis and application potential for trace detection and real-time monitoring.展开更多
The effective and environmentally friendly management of oily wastewater,alongside the beneficial conversion of waste biomass,holds paramount importance for environmental conservation,public health,and sustainable soc...The effective and environmentally friendly management of oily wastewater,alongside the beneficial conversion of waste biomass,holds paramount importance for environmental conservation,public health,and sustainable societal progress.In this research,an innovative biomass core-shell bioreactor(CGC@SiO_(2) aerogel) with selective adsorption and degradation properties was developed.The reactor's core is composed of coffee cellulose aerogel,offering a porous framework conducive to microbial colonization while safeguarding microorganisms from adverse external factors.The shell integrates hydrophobic silica enriched with polydimethylsiloxane,which alters the material's hydrophilic properties,enabling it to remain afloat on water for up to 100 days.This superhydrophobic layer maintained a contact angle of 150° even after ten consecutive rubbings.Experimental results indicate that the material performs exceptionally well in oil-water separation,as demonstrated by its success in 9 consecutive oil-water separations.It achieved 99 % selective adsorption,91 % removal,and 46.2 % degradation of a 3 wt.% diesel solution under conditions of 37℃,120 r/min,and pH=7.Additionally,tests assessing environmental tolerance revealed the material's robust adaptability and stability across varying pH levels and temperatures.Compared to traditional hydrophobic and lipophilic materials or free-floating microorganisms,CGC@SiO2 aerogel not only efficiently captures oil pollutants but also degrades them into non-hazardous substances.Combining biodegradation with selective adsorption has shown to be an effective approach for treating oily wastewater,offering significant practical application potential.The low-carbon production of CGC@SiO2aerogel aligns with circular economy principles,underscoring its role in sustainable development.展开更多
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short...Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.展开更多
Fatigue impacts both mental and physical health,significantly reducing quality of life and daily productivity.Natural bioactive compounds have emerged as promising agents to combat fatigue due to their multifaceted bi...Fatigue impacts both mental and physical health,significantly reducing quality of life and daily productivity.Natural bioactive compounds have emerged as promising agents to combat fatigue due to their multifaceted biological activities and minimal side effects.Key mechanisms through which these compounds exert anti-fatigue effects include enhancing energy metabolism,reducing oxidative stress,supporting mitochondrial integrity,modulating the immune response,and regulating neurotransmitter balance.Plant-derived metabolites such as flavonoids,ginsenosides,saponins,and polysaccharides,as well as animal-based peptides and microbial-derived substances,have demonstrated significant potential in alleviating fatigue symptoms in both clinical and preclinical studies.Additionally,fermented products like kefir,fermented rice bran,and yogurt enhance endurance performance,reduce lactate buildup,and improve glycogen storage,further contributing to fatigue mitigation.As consumer interest in natural alternatives grows,future research should prioritize improving the bioavailability,stability,and targeted delivery of these compounds.This review consolidates recent advances in the understanding of anti-fatigue mechanisms of natural products and highlights emerging directions for their development as functional foods and therapeutic agents.展开更多
基金supported by the Laboratory of Lingnan Modern Agriculture Project, China (NT2021006)the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (32121004)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program, China (2019BT02N054)。
文摘Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect their gut microbiota and resistome. To better understand how continuous antimicrobial use in farm animals alters their microbial ecology, we used a metagenomic approach to investigate the effects of pulsed antimicrobial administration on the bacterial community, antibiotic resistance genes(ARGs) and ARG bacterial hosts in the feces of broiler chickens. Chickens received three 5-day courses of individual or combined antimicrobials, including amoxicillin, chlortetracycline and florfenicol. The florfenicol administration significantly increased the abundance of mcr-1 gene accompanied by floR gene, while amoxicillin significantly increased the abundance of genes encoding the AcrAB-tolC multidrug efflux pump(marA, soxS, sdiA, rob, evgS and phoP).These three antimicrobials all led to an increase in Proteobacteria. The increase in ARG host, Escherichia, was mainly attributed to the β-lactam, chloramphenicol and tetracycline resistance genes harbored by Escherichia under the pulsed antimicrobial treatments. These results indicated that pulsed antimicrobial administration with amoxicillin,chlortetracycline, florfenicol or their combinations significantly increased the abundance of Proteobacteria and enhanced the abundance of particular ARGs. The ARG types were occupied by the multidrug resistance genes and had significant correlations with the total ARGs in the antimicrobial-treated groups. The results of this study provide comprehensive insight into pulsed antimicrobial-mediated alteration of chicken fecal microbiota and resistome.
基金Funding for the study was from Danone Research,Palaiseau,France.MP and DM acknowledge support from METHLAB a FACCE ERA-GAS project in collaboration with the French National Research Agency(ANR)
文摘Direct-fed microbials(DFM) are considered as a promising technique to improve animal productivity without affecting animal health or harming the environment.The potential of three bacterial DFM to reduce methane(CH4)emissions,modulate ruminal fermentation,milk production and composition of primiparous dairy cows was examined in this study.As previous reports have shown that DFM respond differently to different diets,two contrasting diets were used in this study.Eight lactating primiparous cows were randomly divided into two groups that were fed a corn silage-based,high-starch diet(HSD) or a grass silage-based,high-fiber diet(HFD).Cows in each dietary group were randomly assigned to four treatments in a 4 × 4 Latin square design.The bacterial DFM used were selected for their proven CH4-reducing effect in vitro.Treatments included control(without DFM) and 3 DFM treatments: Propionibacterium freudenreichii 53-W(2.9 × 10^10 colony forming units(CFU)/cow per day),Lactobacillus pentosus D31(3.6 × 10^11 CFU/cow per day) and Lactobacillus bulgaricus D1(4.6 × 10^10 CFU/cow per day).Each experimental period included 4 weeks of treatment and 1 week of wash-out,with measures performed in the fourth week of the treatment period.Enteric CH4 emissions were measured during 3 consecutive days using respiration chambers.Rumen samples were collected for ruminal fermentation parameters and quantitative microbial analyses.Milk samples were collected for composition analysis.Body weight of cows were recorded at the end of each treatment period.Irrespective of diet,no mitigating effect of DFM was observed on CH4 emissions in dairy cows.In contrast,Propionibacterium increased CH4 intensity by 27%(g CH4/kg milk) in cows fed HSD.There was no effect of DFM on other fermentation parameters and on bacterial,archaeal and protozoal numbers.Similarly,the effect of DFM on milk fatty acid composition was negligible.Propionibacterium and L.pentosus DFM tended to increase body weight gain with HSD.We conclude that,contrary to the effect previously observed in vitro,bacterial DFM Propionibacterium freudenreichii 53-W,Lactobacillus pentosus D31 and Lactobacillus bulgaricus D1 did not alter ruminal fermentation and failed to reduce CH4 emissions in lactating primiparous cows fed high-starch or high-fiber diets.
文摘BACKGROUND:Breast hyperplasia is a common benign breast disease mainly caused by endocrine disorders,manifested as abnormal hyperplasia of breast tissue.In recent years,traditional Chinese medicine compounds and probiotics have shown good potential in regulating the endocrine system and improving the intestinal microecology,providing new ideas for the treatment of breast hyperplasia.OBJECTIVE:To explore the effects and mechanisms of traditional Chinese medicine compounds and fermented probiotic compounds on breast hyperplasia in mice,providing new theoretical and experimental bases for the clinical treatment and prevention of breast hyperplasia.METHODS:(1)Network pharmacology tools were used to predict the anti-breast-hyperplasia activity of Herba Gueldenstaedtiae(Euphorbia humifusa),as well as its potential targets and signaling pathways.The databases included:TCMSP,OMIM,GeneCards database,UniProt website,Venny2.1.0 website,Metascape,HERB website,and STRING database,all of which are open-access databases.Network pharmacology can predict and screen key information such as the targets corresponding to the active ingredients of traditional Chinese medicine,disease targets,and action pathways through network analysis and computer-system analysis.Therefore,it has been increasingly widely used in the research of traditional Chinese medicine.(2)A breast hyperplasia model was induced in mice by injecting estrogen and progesterone.Mice in the normal blank group were injected intraperitoneally with normal saline every day.Mice in the model group and drugadministration groups were injected intraperitoneally with estradiol benzoate injection at a concentration of 0.5 mg/kg every day for 25 days.From the 26th day,the injection of estradiol benzoate injection was stopped.Mice in the normal blank group were injected intramuscularly with normal saline every day,and mice in the model group and drug-administration groups were injected intramuscularly with progesterone injection at a concentration of 5 mg/kg for 5 days.After the model was established,each group was given drugs respectively.The normal blank group and the model group were gavaged with 0.2 mL/d of normal saline;the positive blank group(Xiaozheng Pill group)was gavaged with an aqueous solution of Xiaozheng Pill at 0.9 mg/g;the low-,medium-and high-dose groups of Compound Herba Gueldenstaedtiae were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively;the low-,medium-and high-dose groups of traditional Chinese medicine-bacteria fermentation were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively.The administration was continuous for 30 days.RESULTS AND CONCLUSION:(1)The results of network pharmacology research showed that the Compound Herba Gueldenstaedtiae(Euphorbia humifusa)contained 46 active ingredients,which were related to 1213 potential targets.After comparison with 588 known breast-hyperplasia targets,it was speculated that 50 of these targets might be related to the direct effect of the compound on breast hyperplasia.(2)After drug intervention,there was no significant change in the high-dose group of Compound Herba Gueldenstaedtiae compared with the normal blank group.The liver indicators of the other intervention groups all significantly decreased(P<0.05).(3)In terms of kidney and uterine indicators,the medium-dose group of Compound Herba Gueldenstaedtiae decreased significantly compared with the normal blank group(P<0.05).In terms of the uterine index,the model group increased significantly compared with the normal blank group(P<0.01).(4)After 1-month drug treatment,the number of lobules and acini in the breast tissue of the Xiaozheng Pill group,the low,medium,and high-dose group of Compound Herba Gueldenstaedtiae,the low,medium,and highdose groups of traditional Chinese medicine-bacteria fermentation decreased,and the duct openings narrowed.With the increase of drug dose,diffuse hyperplasia of breast tissue was significantly improved.(5)The ELISA results showed that compared with the model group,the estrogen level was lower in the medium-dose group of traditional Chinese medicine-bacteria fermentation after the intervention(P<0.05).In addition,the follicle-stimulating hormone level in the low-dose group of Compound Herba Gueldenstaedtiae was lower than that of the model group(P<0.05).(6)The intervention in the mouse model led to changes in the abundance of short chain fatty acids and intestinal flora in all groups.To conclude,the Compound Herba Gueldenstaedtiae and its probiotic fermentation products significantly improved mammary gland hyperplasia in mice by regulating hormone levels,improving the structure of the gut microbiota,and increasing the content of shortchain fatty acids,providing new ideas and potential sources of drugs for the treatment of breast hyperplasia.
基金supported by the European Union-Next Generation EU,Mission 4 Component 1,Project Title:“Gut and Neuro Muscular system:investigating the impact of microbiota on nerve regeneration and muscle reinnervation after peripheral nerve injury”,CUP D53D23007770006,MUR:20227YB93W,to GR。
文摘The gut microbiota:The human body is colonized by a diverse and complex microbial community–including bacteria,viruses,archaea,and unicellular eukaryotes–that plays a central role in human wellbeing.Indeed,microbiota is crucial for several functions,including host metabolism,physiology,maintenance of the intestinal epithelial integrity,nutrition,and immune function,earning it the designation of a“vital organ”(Guinane and Cotter,2013).
基金financially supported by the National Key R&D Program of China(2021YFD1901203 and 2021YFD1901204)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA0440404)+2 种基金the National Natural Science Foundation of China(42377348)the Science Foundation for Distinguished Young Scholars of Hunan Province,China(2024JJ2052)the Natural Science Foundation of Guangxi,China(2025GXNSFAA069337)。
文摘Rapidly improving infertile croplands and enhancing their soil organic carbon(SOC)pool necessitate substantial organic materials incorporation.Converting loose crop straw into granulated form facilitates uniform incorporation within the plough soil layer.As an innovative soil amelioration approach,the efficiency and patterns of SOC accumulation remain unclear.Two field experiments were conducted in infertile subtropical upland and paddy soils with 0,30,60,and 90 Mg ha^(-1)granulated straw incorporation.After one year,SOC accumulation efficiency from straw input remained stable in upland(30.8–37.5%)with increasing amounts of straw incorporation,while declined from 60.0 to 38.3%in paddy.In both croplands,the contributions of lignin phenols to SOC increased with increasing straw incorporation,while the contributions from amino sugars remained constant at higher straw input levels.Subsequently,the ratios of lignin phenols to amino sugars increased with increasing straw incorporation,indicating faster plant residue accumulation compared to microbial necromass,as the granulation approach limited microbial involvement in straw transformation.Thus,single-time incorporation of substantial granulated straw presents an effective agricultural strategy for rapid amelioration of infertile croplands.
基金the Copperbelt University Africa Centre of Excellence for Sustainable Mining(CBUACESM)at Copperbelt University in Kitwe,Zambia for funding this work。
文摘The Earth's environment is undergoing significant transformation due to mining,pollution,and climate change.Although mining is essential for economic development,it contributes significantly to the release of potentially harmful elements(PHEs)that threaten human health and destabilize microbial communities.Anthropogenic climate change,driven by greenhouse gas emissions,alters water availability and soil composition,further affecting ecosystem balance and microbial diversity.This review aggregates findings from studies covering the last two decades to assess how mining pollution and climate change impact microbial diversity,their adaptation mechanisms,and the associated health risks.It reveals that environmental stressors favour resistant microbial taxa while eliminating sensitive species,thereby reshaping microbial ecosystems.Microorganisms demonstrate genetic,biochemical,and physiological adaptations that enable them to survive in polluted or changing environments,often resulting in a higher prevalence of pathogenic and antibiotic-resistant strains.These variations in microbial dynamics contribute to health challenges such as respiratory infections,foodborne illnesses,and increased exposure in children and immune-compromised individuals.The review highlights the linkages between environmental degradation,microbial ecology,and human health,underscoring the need for integrative policies and interventions to mitigate long-term risks,support microbial ecosystem stability,promote sustainable health and agricultural outcomes.
基金supported by the Foundation of National Dairy Technology Innovation Center(2022-Open Funding Project-12)Foundation of National Dairy Technology Innovation Center(2022-Scientific Research-9)+2 种基金Key Project of National Dairy Innovation Research Center of Inner Mongolia(2021-National Dairy Innovation Research Center-8)Key Projects of Research Operating Expenses of Provincial Research Institutes in Heilongjiang Province(CZKYF2021-2-B017)Key Project of Natural Science Foundation of Heilongjiang Province(ZD2022C007).
文摘Suancai has a lengthy history and a wide range of categories,which has some influence on the pickled diet culture around the world.Suancai production is transitioning to a large-scale,standardized production due to the growth of the market.It has a unique flavor and is rich in nutrients,and its abundance of free amino acids,vitamins and phenolics has many positive effects on the human body.This review gives the types and history of suancai,as well as its impact on the world’s pickled culture.The changes in nutritional composition and flavor of suancai during fermentation are summarized.It presented the production technology and influencing factors of the northeast suancai,examined the quality and safety issues in suancai,and put forth some ideas and opinions on the standardization development of the suancai industry.It also summarized the geographic distribution and flora diversity of pickles around the world.In order to provide some knowledge and guidance for the promotion of modern industrial production in the suancai industry.
基金supported by grants from the National Natural Science Foundation of China(No.42207148)the Science and Technology Plan Project of Quanzhou,China(Nos.2025QZNS002 and 2022N030)+2 种基金the Natural Science Foundation of Fujian Province,China(No.2022J01573)the Educational Research Project for Young and Middle-Aged Teachers in Fujian Province,China(No.JAT210042)the Open Project Fund of Key Laboratory of Marine Biological Resources,Ministry of Natural Resources of China(Nos.HY202201 and HY202202)。
文摘Pyrethroids are a class of novel broad-spectrum pesticides synthesized to mimic natural pyrethrins.Due to their high efficiency,low toxicity,and safety,pyrethroids have been widely used as alternatives to organophosphate and carbamate insecticides in the control of agricultural and sanitary pests.However,with the increasing use of pyrethroid pesticides,the resulting pesticide residues have posed threats to both the environment and human health.Biodegradation is considered one of the most promising methods for the removal of pyrethroids,and significant research has been conducted in this area.This review summarizes recent advances in the biodegradation of pyrethroids,including degradation by single strains,microbial consortia,and enzymes.It provides an in-depth analysis of the biodegradation pathways and catalytic mechanisms involved in the degradation of pyrethroids and outlines enhancement strategies for improving the activity of pyrethroid-degrading enzymes.The review also identifies current challenges in pyrethroid biodegradation and offers perspectives for future research.This review serves as a valuable reference for subsequent studies on pyrethroid biodegradation.
基金supported by the National Natural Science Foundation of China(42407458 and 42307420)the Global Challenges Research Fund of the UK Natural Environment Research Council(NE/V005871/1)the Shandong Province First-class Discipline Construction“811”Project,China。
文摘Microplastic accumulation after film mulching affects nutrients cycling in the soil–crop system.Bulk soil(BS)and rhizosphere soil(RS)have two different community compositions which lead to their different microbial nutrient acquisition abilities.Microplastics influence the rhizosphere effect.However,the mechanism by which microplastic accumulation affects the net photosynthetic rate(NPR)through rhizospheric microbial communities remains unknown.This study aimed to identify the mechanisms underlying the effects of polyethylene(PE)and polyvinyl chloride(PVC)microplastics at 0,1,and 5%(w/w)on the NPR in the wheat–soil ecosystem using a pot experiment.Superoxide dismutase(SOD)activity was reduced by 15.35–36.7%,and that of peroxidase(POD)was increased by 32.47–61.93%,causing reductions in NPR(17.94–23.81%)in the PE5%and PVC(1 and 5%)(w/w)treatments compared with the control.The Chao1,Shannon,and Simpson indices of the bacterial and fungal diversities were lower in BS than in RS at PE1%and PVC5%(w/w),respectively.The bacterial and fungal network complexities were reduced and increased,respectively,owing to alterations in the bacterial and fungal community compositions and structures for wheat growth.The Mantel test showed that the bacterial and fungal diversity indices in BS had positive correlations with Olsen-P and phosphatase;however,those in RS were positively correlated with NO_(3)^(–) and β-1,4-glucosidase.The structural equation model indicated that wheat enzymatic and soil hydrolytic activities negatively affected NPR.Wheat has a profound antioxidant defense strategy for PE and PVC microplastic stress,which produces a synergistic effect of POD by protecting organelles and reducing tissue damage to preserve the NPR.
基金supported by China Hunan Provincial Science&Technology Department(No.2023NK2031)the Natural Science Foundation of Hunan Province(No.2023JJ40031)the Ministry of Human Resources and Social Security(No.H20240365).
文摘To explore the adaptive mechanisms of the partial nitritation-anammox(PNA)process under high salinity stress during kitchen wastewater treatment,focusing on their physiological and molecular responses through metagenomic analysis.An airlift inner-circulation partition bioreactor(AIPBR)was developed,featuring an inner cylinder and a flow guide tube to create distinct oxygen gradients,facilitating the study of microbial adaptation under varying salt conditions.The AIPBR was operated with synthetic wastewater containing ammonium concentrations of 1800±100 mg/L and salinity gradients ranging from 1 to 10 g/L,followed by a fixed salinity period at 6 g/L,with ammonium concentrations approximately 850 mg/L.High-throughput metagenomic analysis revealed shifts in functional genes and metabolic pathways in response to salinity stress.Anammox bacteria adapted by enriching genes involved in the synthesis of osmoprotective compounds and activating energy-producing pathways like the tricarboxylic acid cycle(TCA).These adaptations,along with modifications in membrane composition,were essential for sustaining system stability under elevated salinity.Under prolonged high salinity stress,anaerobic ammonium oxidizing(AnAOB)exhibited improved salt tolerance,maintaining a total nitrogen removal efficiency above 85%and stabilizing after an adaptation phase.The metagenomic data revealed a marked enrichment of genes associated with ion transport,stress response mechanisms,and DNA repair pathways.Changes in microbial community composition favored salt-tolerant species,supporting system stability.These findings highlight the applicability of the developed bioreactor for scaling up the PNA process to handle high-salinity wastewater,providing a promising avenue for sustainable nitrogen removal in challenging environments.
基金supported by the China Central Government-Guided Local Science and Technology Development Project(23ZYQA291)the Innovation Star Project for Excellent Postgraduates in Gansu Province(2025CXZX-169)the Key Science&Technology Project of Gansu Province,China(22ZD6NA007)。
文摘Soil water content and salinity critically regulate soil microbial composition,plant community structure,and ecosystem multifunctionality(EMF)in semi-arid grasslands.However,the mechanisms through which drought(D),saline-alkaline(SA),and their combined(DSA)stress influence these ecological components remain poorly understood.This study investigated these mechanisms along natural gradients in a semi-arid grassland of China by analyzing soil physical-chemical properties,microbial communities,and vegetation characteristics.The results showed that as the environmental stress shifted from the D group to the DSA group and then to the SA group,soil electrical conductivity significantly increased,while urease and phosphatase activities significantly decreased.Soil organic carbon,total nitrogen,total phosphorus,and microbial biomass carbon and nitrogen were lower in the D and SA groups than in the DSA group.Meanwhile,plant biomass showed an increasing trend along the treatment gradient,primarily driven by dominant species,while plant diversity did not exhibit significant differences.Further analysis identified the soil water content and salinity as the key determinants of soil microbial diversity and community complexity.Soil enzyme activities exhibited contrasting relationships with microbial composition,correlating positively with the richness of bacterial amplicon sequence variants(ASVs)but negatively with the richness of fungal ASVs.Notably,microbial biomass,which varied significantly across different groups,emerged as a key predictor of changes in EMF,with its critical role confirmed through structural equation modeling.These findings collectively elucidate the responses of ecological communities to synergistic soil hydro-saline stress in semi-arid ecosystems,while highlighting the critical role of microbial biomass in maintaining EMF.
基金supported by the National Key R&D Program of China(No.2023YFC3706703)the National Natural Science Foundation of China(No.51874018)。
文摘The remediation of arsenic(As)-contaminated soil is essential for achieving sustainable environmental and agricultural development.Among various techniques,soil washing has emerged as a promising method due to its rapid,efficient,and thorough decontamination capabilities.This review critically examines the application of soil washing technology in the treatment of As-contaminated soil.Specifically,this paper discusses the mechanisms of four types of washing agents(inorganic detergents,chelating agents,surfactants,and microbial agents),focusing on processes such as acid dissolution,electrostatic interaction,ion exchange,and chelation,and the factors affecting washing efficiency.The concentration of washing agent and the initial p H are the key factors influencing the washing effect.The paper also summarizes the application conditions and the corresponding removal rates for different individual washing agents and compares their effectiveness,biodegradability,and environmental impacts.Among these,natural chelating agents are highlighted for their promising potential in As removal.While individual washing agents show certain effectiveness,the combined use of multiple washing agents and the optimization of washing sequence are necessary to achieve superior remediation outcomes.The synergistic effects of combining natural chelating agents with reducing agents,surfactants,and inorganic washing agents,as well as the integration of nanomaterials with chelating agents and microbial agents are summarized,demonstrating their efficiency and stability in soil remediation.By reviewing the current state of research,this paper provides essential insights for the selection of washing agents and the optimization of washing parameters in the remediation of As-contaminated soil.
基金funded by the Beef Cattle Research Council Cluster(FDE.18.21C)Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery,NSERC Canadian Research Chair(Tier 1)program+2 种基金NSERC Alliance program(ALLRP 588541‐23)Foundation for Food&Agriculture Research Greener Cattle Initiative(Award ID 22‐000373)DSM Nutritional Products,Kaiseraugst,Switzerland。
文摘Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied.This study investigated changes in rumen bacteria,archaea,and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.Results A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation.The ST study(95 samples)used eight ruminally cannulated beef cattle in a 4×4 Latin square design with four 28-d of 3-NOP treatments[mg/kg of dry matter(DM)]:control:0,low:53,med:161,and high:345.The LT study(48 samples)was a completely randomized design with two 3-NOP treatments[control:0,and high:280 mg/kg of DM)fed for 112-d followed by a 16-d withdrawal(without 3-NOP).Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed.Under ST supplementation,the relative abundances of Prevotella,Methanobrevibacter(Mbb.)ruminantium,Methanosphaera sp.ISO3-F5,and Entodinium were increased(Q<0.05),whereas those of Mbb.gottschalkii and Epidinium were decreased(Q<0.05)with 3-NOP supplementation.In LT study,relative abundances of Mbb.ruminantium,and Methanosphaera sp.Group5 were increased(Q<0.05),while those of Saccharofermentans and Mbb.gottschalkii were decreased(Q<0.05)with 3-NOP supplementation.Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp.Group5(P<0.05)after 3-NOP withdrawal.Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher(P=0.029)relative abundances of Eggerthellaceae DNF00809,p-1088-a5 gut group,and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa.Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.Conclusions Both ST and LT supplementation affected overall rumen microbial profile,with individual microbial groups responded to 3-NOP supplementation differently.After 3-NOP withdrawal,not all microbes showed recovery,indicating that the 3-NOP driven shifts were only partially reversible.These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.
基金supported by the Key Research and Development Program of Ningxia Hui Autonomous Region,China(2023BCF01029)the Shaanxi Provincial Science and Technology Project for Innovation Team,China(2023-CXTD-59)the Innovative Team Special Project of Northwest A&F University,China(XYTD2023-12)。
文摘Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon'grapes sourced from organic and conventional management vineyard(OMV/CMV)in the Ningxia region were subjected to spontaneous fermentation.The microbial,oenological,and aroma profiles of grape must and resulting wines were assessed using high-throughput sequencing(HTS),high-performance liquid chromatography(HPLC),gas chromatography with mass spectrometry(GC-MS),and sensory evaluations.Network analysis was applied to explore relationships among microorganisms,volatile compounds,and aroma attributes.Results showed that organic management significantly increased microbial species richness,α-diversity,and the variety and concentration of aroma compounds,favoring the production of natural wines with complex aroma profiles.Relative abundance of Saccharomyces in OMV reduced,promoting the prevalence of other yeast species during fermentation.Bacterial succession in wines from OMV remained stable,with Pantoea as the dominant genus.Among oenological parameters,OMV wines significantly induced glycerol content,while reduced total acidity,tartaric acid,and citric acid content.These wines exhibited significantly higher levels of fermentative(+16%)and varietal(+72%)volatiles,as well as enhanced floral and sweet fruity aromas,along with distinct nail polish and vegetal notes.Additionally,Saccharomyces,Hanseniaspora,Metschnikowia,and Pantoea were strongly correlated with specific volatile compounds and aroma characteristics.This study provides valuable data that can inform spontaneous fermentation practices and guide vineyard management for natural wine production.
基金supported by the National Natural Science Foundation of China(Grant No.32160172)the Key Science-Technology Project of Inner Mongolia(2023KYPT0010)+1 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region of China(Grant No.2025QN03006)the 2023 Inner Mongolia Public Institution High-level Talent Introduction Scientific Research Support Project.
文摘This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from the Dahei River prior to DNA extraction and 16S rRNA gene sequencing,it generates standard curves to convert sequencing data into absolute microbial copy numbers.The method,which is proved highly accurate(R^(2)>0.99),reveals a clear contrast between the river sites:the upstream community has not only a significantly higher total microbial load but also a completely different makeup of species compared to the downstream site.This approach effectively overcomes the limitations of relative abundance analysis,providing a powerful tool for environmental monitoring,and proposes key steps for future standardization to ensure data comparability and integration.
基金supported by the National Key R&D Program of China(No.2023YFD2401703)。
文摘The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litopenaeus vannamei as a model species and two strains of Bacillus(W1 and XYB4)combined with sodium humate under zero-water exchange conditions.The growth performance,enzyme activity,and aquatic system microbial environment of L.vannamei were analyzed.Results showed that the combination of sodium humate and Bacillus strains effectively enhanced environmental conditions for the growth and reproduction of heterotrophic bacteria while inhibiting the growth of Vibrio species,including green and yellow variants.Microbiome analysis showed that the group treated with Bacillus strains combined with sodium humate exhibited significantly higher relative abundances of Firmicutes and Actinobacteriota than the other groups.Correspondingly,this treatment group showed substantially enhanced weight gain rate,specific growth rate,survival rate,and feed coefficient.Moreover,the phenol oxidase,catalase,lysozyme,and superoxide dismutase indexes of shrimps subjected to Bacillus–sodium humate treatment were considerably higher than those of the control group.These findings confirm that the combination of Bacillus and sodium humate has beneficial effects on shrimp growth and aquatic system quality control,providing a promising strategy for enhancing the efficiency of shrimp farming and aquaculture.
基金supported by the Bingtuan Industrial Technology Research Institute,Bingtuan New materials Research Institute innovation platform project,Research initiation project of Shihezi University(No.RCZK202330)the Science and Technology Program-Regional Innovation Guidance Program(No.2023ZD080)Tianchi Talent Project(No.CZ002735).
文摘The microbial degradation of aromatic organic pollutants is incomplete due to their metabolic characteristics,which can easily produce certain highly toxic intermediates.Therefore,this article designs a dual template molec-ularly imprinted sensor(DTMIP/Fe-Mn@C)for iron manganese metal nanomaterials,prepared Fe-Mn@C com-posite materials by a one pot method were coated on the surface of glassy carbon electrodes and covered with molecularly imprinted membranes through electropolymerization and elution methods,achieving real-time de-tection of specific intermediate products 2-methylbutyric acid(2-MBA)and 3-methylbutyric acid(3-MBA)de-graded by azo dyes.In order to determine the detection sensitivity and intensity range of the sensor,optimization experiments were conducted on various parameters that affect the detection performance,such as the type of func-tional monomer and its composition ratio with the template molecule,detection time window,environmental pH value,etc.Finally,o-Phenylenediamine was determined as the functional monomer,with a molar ratio of 1:1:6 to the template molecules 2-MBA and 3-MBA.Electrochemical testing was conducted in a neutral environment with an incubation time of 5 min and pH=7.The results indicate that the sensor has a relatively wide detection range,high sensitivity,obvious recognition features,and excellent stability for 2-MBA and 3-MBA.This new dual template molecularly imprinted sensor can quickly and accurately determine the safety of highly toxic interme-diates in the degradation process of aromatic organic pollutants,providing a theoretical basis and application potential for trace detection and real-time monitoring.
基金supported by the National Natural Science Foundation of China(Nos.22365026 and 21966028)the Science and Technology Project of Gansu(No.21YF5GA062)+3 种基金the Fundamental Research Funds for the Central Universities(Nos.31920220043,31920240094,and 31920230142)the Education Department of Gansu Province:Excellent Graduate student“Innovation Star”project(No.2023CXZX-202)Gansu Province Science Foundation for Youths(No.24JRRA160)the Funds for Special Projects of the Central Government in Guidance of Local Science and Technology Development(No.24ZY1QA026).
文摘The effective and environmentally friendly management of oily wastewater,alongside the beneficial conversion of waste biomass,holds paramount importance for environmental conservation,public health,and sustainable societal progress.In this research,an innovative biomass core-shell bioreactor(CGC@SiO_(2) aerogel) with selective adsorption and degradation properties was developed.The reactor's core is composed of coffee cellulose aerogel,offering a porous framework conducive to microbial colonization while safeguarding microorganisms from adverse external factors.The shell integrates hydrophobic silica enriched with polydimethylsiloxane,which alters the material's hydrophilic properties,enabling it to remain afloat on water for up to 100 days.This superhydrophobic layer maintained a contact angle of 150° even after ten consecutive rubbings.Experimental results indicate that the material performs exceptionally well in oil-water separation,as demonstrated by its success in 9 consecutive oil-water separations.It achieved 99 % selective adsorption,91 % removal,and 46.2 % degradation of a 3 wt.% diesel solution under conditions of 37℃,120 r/min,and pH=7.Additionally,tests assessing environmental tolerance revealed the material's robust adaptability and stability across varying pH levels and temperatures.Compared to traditional hydrophobic and lipophilic materials or free-floating microorganisms,CGC@SiO2 aerogel not only efficiently captures oil pollutants but also degrades them into non-hazardous substances.Combining biodegradation with selective adsorption has shown to be an effective approach for treating oily wastewater,offering significant practical application potential.The low-carbon production of CGC@SiO2aerogel aligns with circular economy principles,underscoring its role in sustainable development.
基金supported by the National Research Foundation of Korea(2020R1F1A1074155).
文摘Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.
基金supported by Guangdong Higher Education Upgrading Plan(2021-2025)with No.of UICR0400015-24 and UICR0400016-24 at Beijing Normal-Hong Kong Baptist University,Zhuhai,China.
文摘Fatigue impacts both mental and physical health,significantly reducing quality of life and daily productivity.Natural bioactive compounds have emerged as promising agents to combat fatigue due to their multifaceted biological activities and minimal side effects.Key mechanisms through which these compounds exert anti-fatigue effects include enhancing energy metabolism,reducing oxidative stress,supporting mitochondrial integrity,modulating the immune response,and regulating neurotransmitter balance.Plant-derived metabolites such as flavonoids,ginsenosides,saponins,and polysaccharides,as well as animal-based peptides and microbial-derived substances,have demonstrated significant potential in alleviating fatigue symptoms in both clinical and preclinical studies.Additionally,fermented products like kefir,fermented rice bran,and yogurt enhance endurance performance,reduce lactate buildup,and improve glycogen storage,further contributing to fatigue mitigation.As consumer interest in natural alternatives grows,future research should prioritize improving the bioavailability,stability,and targeted delivery of these compounds.This review consolidates recent advances in the understanding of anti-fatigue mechanisms of natural products and highlights emerging directions for their development as functional foods and therapeutic agents.
