Themulti-soil-layering(MSL)systems is an emerging solution for environmentally-friendly and cost-effective treatment of decentralized rural domestic wastewater.However,the role of the seemingly simple permeable layer ...Themulti-soil-layering(MSL)systems is an emerging solution for environmentally-friendly and cost-effective treatment of decentralized rural domestic wastewater.However,the role of the seemingly simple permeable layer has been overlooked,potentially holding the breakthroughs or directions to addressing suboptimal nitrogen removal performance in MSL systems.In this paper,the mechanism among diverse substrates(zeolite,green zeolite and biological ceramsite)coupled microorganisms in different systems(activated bacterial powder and activated sludge)for rural domestic wastewater purification was investigated.The removal efficiencies performed by zeolite coupled with microorganisms within 3 days were 93.8%for COD,97.1%for TP,and 98.8%for NH_(4)^(+)-N.Notably,activated sludge showed better nitrification and comprehensive performance than specialized nitrifying bacteria powder.Zeolite attained an impressive 89.4%NH4+-N desorption efficiency,with a substantive fraction of NH_(4)^(+)-N manifesting as exchanged ammonium.High-throughput 16S rRNA gene sequencing revealed that aerobic and parthenogenetic anaerobic bacteria dominated the reactor,with anaerobic bacteria conspicuously absent.And the heterotrophic nitrificationaerobic denitrification(HN-AD)process was significant,with the presence of denitrifying phosphorus-accumulating organisms(DPAOs)for simultaneous nitrogen and phosphorus removal.This study not only raises awareness about the importance of the permeable layer and enhances comprehension of the HN-AD mechanism in MSL systems,but also provides valuable insights for optimizing MSL system construction,operation,and rural domestic wastewater treatment.展开更多
Microbial communities play key roles in the marine ecosystem. Despite a few studies on marine microbial communities in deep straits, ecological associations among microbial communities in the sediments of shallow stra...Microbial communities play key roles in the marine ecosystem. Despite a few studies on marine microbial communities in deep straits, ecological associations among microbial communities in the sediments of shallow straits have not been fully investigated. The Bohai Strait in northern China(average depth less than 20 m) separates the Bohai Sea from the Yellow Sea and has organic-rich sediments. In this study, in the summer of 2014, six stations across the strait were selected to explore the taxonomic composition of microbial communities and their ecological associations. The four most abundant classes were Gammaproteobacteria, Deltaproteobacteria, Bacilli and Flavobacteriia. Temperature, total carbon, depth, nitrate, fishery breeding and cold water masses influenced the microbial communities, as suggested by representational dif ference and composition analyses. Network analysis of microbial associations revealed that key families included Flavobacteriaceae, Pirellulaceae and Piscirickettsiaceae. Our findings suggest that the families with high phylogenetic diversity are key populations in the microbial association network that ensure the stability of microbial ecosystems. Our study contributes to a better understanding of microbial ecology in complex hydrological environments.展开更多
The present study investigated the effects of cow manure ratios mixed with maize stover,rice straw,and wheat stalk at 3,2,1(total solid based,TS-based),respectively,on methane production and microbial community struct...The present study investigated the effects of cow manure ratios mixed with maize stover,rice straw,and wheat stalk at 3,2,1(total solid based,TS-based),respectively,on methane production and microbial community structure during the anaerobic co-digestion process.Results showed cow manure co-digested with maize stover,wheat stalk,and rice straw at ratios of 2,1,and 3 had the highest cumulative methane yields(272.99,153.22167.73 mL/g volatile solid(VS),respectively)and better stability(e.g.pH,volatile fatty acids(VFAs)and their component).The main microbe evolution had a similar trend which was Petrimonas and Methanosaeta in the early digestion process(Days 0-7)and then evolved into Longilinea,Ruminofilibacter,and Methanosarcina with the progress of digestion,but the relative abundance of these microbes in each reactor was different.It was worth noting that Caldicoprobacter in cow manure to maize stover ratio of 2,and to rice straw ratio of three reactors had a relatively higher proportion than reactor of cow manure to wheat stalk ratio of 1,and Hydrogenophaga was the specific bacterium in cow manure to wheat stalk ratio of 1 reactor.In addition,Petrimonas showed positive relationship with VFAs and Longilinea was the opposite.Methanosaeta and Methanobacterium contributed the most during the peak period of methane production in cow manure and maize stover co-digested reactor,and showed positive relationship with acetic acid.However,Methanosarcina and Methanospirillum made a great contribution during the peak period of methane production in cow manure co-digested with wheat stalk and rice straw reactors.These findings could provide further information on the application of cow manure co-digested with crop wastes.展开更多
1 Introduction Genomic islands(GIs)promote co-evolution among different species,which are of significant importance for studying microbial functionality and evolution[1].To effectively identify genomic islands,numerou...1 Introduction Genomic islands(GIs)promote co-evolution among different species,which are of significant importance for studying microbial functionality and evolution[1].To effectively identify genomic islands,numerous algorithms have been developed.These methods detect candidate genomic islands either by recognizing differences in sequence composition between genomic islands and their host genomes or by analyzing multiple genomes through sequence alignment[2].However,it’s important to note that genomic islands are formed through horizontal gene transfer and typically contain functional genes,such as those related to pathogenicity,metabolic regulation,and antibiotic resistance,as well as mobile elements like insertion sequences(IS),integrative and conjugative elements(ICE),mobile genetic elements(MGE),and bacteriophages(phage).In turn,utilizing these mobile elements can aid in the identification of genomic islands.展开更多
Geomicrobiology is a sub-discipline of geobiology and emphasizes the interaction between microorganisms and their environment on Earth. There is a need to explicitly emphasize the biogeochemical processes performed by...Geomicrobiology is a sub-discipline of geobiology and emphasizes the interaction between microorganisms and their environment on Earth. There is a need to explicitly emphasize the biogeochemical processes performed by microorganisms associated with Earth's tectonic activities, especially under the framework of the modern theory of plate tectonics. Tectonomicrobiology aims to create a better synergy between microbial and active tectonic processes. This explicit synergy should also foster better communications between solid Earth scientists and life scientists in terms of holistic Earth system dynamics at both tectonic and micro-scales.展开更多
基金supported by the National Natural Science Foundation of China(No.42077163).
文摘Themulti-soil-layering(MSL)systems is an emerging solution for environmentally-friendly and cost-effective treatment of decentralized rural domestic wastewater.However,the role of the seemingly simple permeable layer has been overlooked,potentially holding the breakthroughs or directions to addressing suboptimal nitrogen removal performance in MSL systems.In this paper,the mechanism among diverse substrates(zeolite,green zeolite and biological ceramsite)coupled microorganisms in different systems(activated bacterial powder and activated sludge)for rural domestic wastewater purification was investigated.The removal efficiencies performed by zeolite coupled with microorganisms within 3 days were 93.8%for COD,97.1%for TP,and 98.8%for NH_(4)^(+)-N.Notably,activated sludge showed better nitrification and comprehensive performance than specialized nitrifying bacteria powder.Zeolite attained an impressive 89.4%NH4+-N desorption efficiency,with a substantive fraction of NH_(4)^(+)-N manifesting as exchanged ammonium.High-throughput 16S rRNA gene sequencing revealed that aerobic and parthenogenetic anaerobic bacteria dominated the reactor,with anaerobic bacteria conspicuously absent.And the heterotrophic nitrificationaerobic denitrification(HN-AD)process was significant,with the presence of denitrifying phosphorus-accumulating organisms(DPAOs)for simultaneous nitrogen and phosphorus removal.This study not only raises awareness about the importance of the permeable layer and enhances comprehension of the HN-AD mechanism in MSL systems,but also provides valuable insights for optimizing MSL system construction,operation,and rural domestic wastewater treatment.
基金Supported by the Strategic Priority Research Program of Chinese Academic of Sciences(No.XDA1102040303)the National Basic Research Program of China(973 Program)(No.2015CB453300)
文摘Microbial communities play key roles in the marine ecosystem. Despite a few studies on marine microbial communities in deep straits, ecological associations among microbial communities in the sediments of shallow straits have not been fully investigated. The Bohai Strait in northern China(average depth less than 20 m) separates the Bohai Sea from the Yellow Sea and has organic-rich sediments. In this study, in the summer of 2014, six stations across the strait were selected to explore the taxonomic composition of microbial communities and their ecological associations. The four most abundant classes were Gammaproteobacteria, Deltaproteobacteria, Bacilli and Flavobacteriia. Temperature, total carbon, depth, nitrate, fishery breeding and cold water masses influenced the microbial communities, as suggested by representational dif ference and composition analyses. Network analysis of microbial associations revealed that key families included Flavobacteriaceae, Pirellulaceae and Piscirickettsiaceae. Our findings suggest that the families with high phylogenetic diversity are key populations in the microbial association network that ensure the stability of microbial ecosystems. Our study contributes to a better understanding of microbial ecology in complex hydrological environments.
基金financially supported by the Youth Natural Science Foundation of Hebei province(Grant No.E2020204023)the Talents Introduction Plan(Grant No.YJ201831)of the Hebei Agricultural University+1 种基金the Key R&D projects of Hebei Province(Grant No.19223811D)the Forestry discipline project of Hebei Agricultural University(Grant No.XK1008601579).
文摘The present study investigated the effects of cow manure ratios mixed with maize stover,rice straw,and wheat stalk at 3,2,1(total solid based,TS-based),respectively,on methane production and microbial community structure during the anaerobic co-digestion process.Results showed cow manure co-digested with maize stover,wheat stalk,and rice straw at ratios of 2,1,and 3 had the highest cumulative methane yields(272.99,153.22167.73 mL/g volatile solid(VS),respectively)and better stability(e.g.pH,volatile fatty acids(VFAs)and their component).The main microbe evolution had a similar trend which was Petrimonas and Methanosaeta in the early digestion process(Days 0-7)and then evolved into Longilinea,Ruminofilibacter,and Methanosarcina with the progress of digestion,but the relative abundance of these microbes in each reactor was different.It was worth noting that Caldicoprobacter in cow manure to maize stover ratio of 2,and to rice straw ratio of three reactors had a relatively higher proportion than reactor of cow manure to wheat stalk ratio of 1,and Hydrogenophaga was the specific bacterium in cow manure to wheat stalk ratio of 1 reactor.In addition,Petrimonas showed positive relationship with VFAs and Longilinea was the opposite.Methanosaeta and Methanobacterium contributed the most during the peak period of methane production in cow manure and maize stover co-digested reactor,and showed positive relationship with acetic acid.However,Methanosarcina and Methanospirillum made a great contribution during the peak period of methane production in cow manure co-digested with wheat stalk and rice straw reactors.These findings could provide further information on the application of cow manure co-digested with crop wastes.
基金supported by the National Natural Science Foundation of China(Grant Nos.62172369,62302456)the Special Support Plan for High Level Talents in Zhejiang Province(2021R52019).
文摘1 Introduction Genomic islands(GIs)promote co-evolution among different species,which are of significant importance for studying microbial functionality and evolution[1].To effectively identify genomic islands,numerous algorithms have been developed.These methods detect candidate genomic islands either by recognizing differences in sequence composition between genomic islands and their host genomes or by analyzing multiple genomes through sequence alignment[2].However,it’s important to note that genomic islands are formed through horizontal gene transfer and typically contain functional genes,such as those related to pathogenicity,metabolic regulation,and antibiotic resistance,as well as mobile elements like insertion sequences(IS),integrative and conjugative elements(ICE),mobile genetic elements(MGE),and bacteriophages(phage).In turn,utilizing these mobile elements can aid in the identification of genomic islands.
基金supported by the National Natural Science Foundation of China(Grant Nos.41530105,41373072,91628301&U1606401)the Chinese Academy of Sciences(Grant Nos.Y4SL021001&QYZDY-SSW-DQC005)the Southern University of Science and Technology(Grant No.Y01316209)
文摘Geomicrobiology is a sub-discipline of geobiology and emphasizes the interaction between microorganisms and their environment on Earth. There is a need to explicitly emphasize the biogeochemical processes performed by microorganisms associated with Earth's tectonic activities, especially under the framework of the modern theory of plate tectonics. Tectonomicrobiology aims to create a better synergy between microbial and active tectonic processes. This explicit synergy should also foster better communications between solid Earth scientists and life scientists in terms of holistic Earth system dynamics at both tectonic and micro-scales.
