A new pressure-calibration method for calibrating the reduction of second harmonic(2f)amplitude caused by pressure broadening effect in sealed microbial growth environment is present.The new method combines with linew...A new pressure-calibration method for calibrating the reduction of second harmonic(2f)amplitude caused by pressure broadening effect in sealed microbial growth environment is present.The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO_(2) in microbial growth.In order to verify the method,a simulation experiment is developed,in which the increasing CO_(2) concentration leads to the increasing pressure.Comparing with the relation between the traditional 2f amplitude and gas concentration,there is a monotonous relation between the calibrated 2f amplitude and CO_(2) concentration,particularly,a linear relation is present when the CO_(2) concentration is replaced with the CO_(2) particle number.In terms of microbial measurement,the growth of Escherichia coli is measured,and the culture bottle is sealed during the microbial growth process.The experimental results show that,comparing to the microbial growth retrieved by traditional 2f amplitude,the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.展开更多
High concentrations of environmental fungi in the archives repositories are dangerous for the documents preserved in those places and for the workers'health.The aims of this work were to evaluate the behavior of t...High concentrations of environmental fungi in the archives repositories are dangerous for the documents preserved in those places and for the workers'health.The aims of this work were to evaluate the behavior of the fungal concentration and diversity in the indoor air of repositories of 3 archives located in Havana,Cuba,and to demonstrate the potential risk that these taxa represent for the documentary heritage preserved in these institutions.The indoor and outdoor environments were sampled with a biocollector.From the I/O ratios,it was evident that two of the studied archives were not contaminated,while one of them did show contamination despite having temperature and relative humidity values very similar to the other two.Aspergillus,Penicillium and Cladosporium were the predominant genera in the indoor environments.New finds for archival environments were the genera Harposporium and Scolecobasidium.The principal species classified ecologically as abundant were C.cladosporioides and P.citrinum.They are known as opportunistic pathogenic fungi.All the analyzed taxa excreted acids,the most of them degraded cellulose,starch and gelatin while about 48%excreted different pigments.But 33%of them showed the highest biodeteriogenic potential,evidencing that they are the most dangerous for the documentary collections.展开更多
Corrosion test data were measured using non-destructive electrochemical techniques and analysed for studying inhibition effectiveness by different concentrations of NazCr207 on the corrosion of concrete steel-rehar in...Corrosion test data were measured using non-destructive electrochemical techniques and analysed for studying inhibition effectiveness by different concentrations of NazCr207 on the corrosion of concrete steel-rehar in NaC1 and in H2SO4 media. For these, specifications of ASTM G16-95 R04 were combined with the normal and the Gumbel probability density functions as model analytical methods for addressing issues of conflicting reports of inhibitor effectiveness that had generated concerns. Results show that reinforced concrete samples admixed with concentrations having 4 g （0.012 7 tool）, 8 g （0.025 4 mol） and 6 g （0.019 l tool） NaaCr207 exhibited, in that order, high inhibition effectiveness, with respective efficiency, r/, of （90.46±1.30）%, （88.41＋2.24）% and （84.87±4.74）%, in the NaC1 medium. These exhibit good agreements within replicates and statistical methods for the samples. Also, optimal inhibition effectiveness model in the H2SO4 medium was exhibited by 8 g （0.025 4 mol） Na2Cr207 concentration having r/=（78.44±1.10）%. These bear implications for addressing conflicting test data in the study of effective inhibitors for mitigating steel-rebar corrosion in aggressive environments.展开更多
Intervening in the microbial environment holds promise for enhancing antitumor efficacy by reshaping the tumor microenvironment,yet few strategies have been reported.In a study led by Zou and coworkers,oral hydrogels ...Intervening in the microbial environment holds promise for enhancing antitumor efficacy by reshaping the tumor microenvironment,yet few strategies have been reported.In a study led by Zou and coworkers,oral hydrogels are introduced to regulate the microbiota balance in the intestines and tumors,triggering an antitumor immune response.This work presents a microbiota-targeted drug delivery system that demonstrates notable efficacy in colon targeting and colon retention for the treatment of colorectal cancer.This represents a significant clinical advancement in treating colorectal cancer,which is particularly vulnerable to microbial infitration.展开更多
The intestinal and intratumoral microbiota are closely associated with tumor progression and response to antitumor treatments.The antibacterial or tumor microenvironment(TME)-modulating approaches have been shown to m...The intestinal and intratumoral microbiota are closely associated with tumor progression and response to antitumor treatments.The antibacterial or tumor microenvironment(TME)-modulating approaches have been shown to markedly improve antitumor efficacy,strategies focused on normalizing the microbial environment are rarely reported.Here,we reported the development of an orally administered inulin-based hydrogel with colon-targeting and retention effects,containing hollow MnO^(2) nanocarrier loaded with the chemotherapeutic drug Oxa(Oxa@HMI).On the one hand,beneficial bacteria in the colon specifically metabolized Oxa@HMI,resulting in the degradation of inulin and the generation of short-chain fatty acids(SCFAs).These SCFAs play a crucial role in modulating microbiota and stimulating immune responses.On the other hand,the hydrogel matrix underwent colon microbiota-specific degradation,enabling the targeted release of Oxa and production of reactive oxygen species in the acidic TME.In this study,we have established,for the first time,a microbiota-targeted drug delivery system Oxa@HMI that exhibited high efficiency in colorectal cancer targeting and colon retention.Oxa@HMI promoted chemotherapy efficiency and activated antitumor immune responses by intervening in the microbial environment within the tumor tissue,providing a crucial clinical approach for the treatment of colorectal cancer that susceptible to microbial invasion.展开更多
基金the National Key Research and Development Program of China(Grant Nos.2017YFC0209700 and 2016YFC0303900)the National Natural Science Foundation of China(Grant No.41730103).
文摘A new pressure-calibration method for calibrating the reduction of second harmonic(2f)amplitude caused by pressure broadening effect in sealed microbial growth environment is present.The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO_(2) in microbial growth.In order to verify the method,a simulation experiment is developed,in which the increasing CO_(2) concentration leads to the increasing pressure.Comparing with the relation between the traditional 2f amplitude and gas concentration,there is a monotonous relation between the calibrated 2f amplitude and CO_(2) concentration,particularly,a linear relation is present when the CO_(2) concentration is replaced with the CO_(2) particle number.In terms of microbial measurement,the growth of Escherichia coli is measured,and the culture bottle is sealed during the microbial growth process.The experimental results show that,comparing to the microbial growth retrieved by traditional 2f amplitude,the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.
文摘High concentrations of environmental fungi in the archives repositories are dangerous for the documents preserved in those places and for the workers'health.The aims of this work were to evaluate the behavior of the fungal concentration and diversity in the indoor air of repositories of 3 archives located in Havana,Cuba,and to demonstrate the potential risk that these taxa represent for the documentary heritage preserved in these institutions.The indoor and outdoor environments were sampled with a biocollector.From the I/O ratios,it was evident that two of the studied archives were not contaminated,while one of them did show contamination despite having temperature and relative humidity values very similar to the other two.Aspergillus,Penicillium and Cladosporium were the predominant genera in the indoor environments.New finds for archival environments were the genera Harposporium and Scolecobasidium.The principal species classified ecologically as abundant were C.cladosporioides and P.citrinum.They are known as opportunistic pathogenic fungi.All the analyzed taxa excreted acids,the most of them degraded cellulose,starch and gelatin while about 48%excreted different pigments.But 33%of them showed the highest biodeteriogenic potential,evidencing that they are the most dangerous for the documentary collections.
文摘Corrosion test data were measured using non-destructive electrochemical techniques and analysed for studying inhibition effectiveness by different concentrations of NazCr207 on the corrosion of concrete steel-rehar in NaC1 and in H2SO4 media. For these, specifications of ASTM G16-95 R04 were combined with the normal and the Gumbel probability density functions as model analytical methods for addressing issues of conflicting reports of inhibitor effectiveness that had generated concerns. Results show that reinforced concrete samples admixed with concentrations having 4 g （0.012 7 tool）, 8 g （0.025 4 mol） and 6 g （0.019 l tool） NaaCr207 exhibited, in that order, high inhibition effectiveness, with respective efficiency, r/, of （90.46±1.30）%, （88.41＋2.24）% and （84.87±4.74）%, in the NaC1 medium. These exhibit good agreements within replicates and statistical methods for the samples. Also, optimal inhibition effectiveness model in the H2SO4 medium was exhibited by 8 g （0.025 4 mol） Na2Cr207 concentration having r/=（78.44±1.10）%. These bear implications for addressing conflicting test data in the study of effective inhibitors for mitigating steel-rebar corrosion in aggressive environments.
基金support from School of Chemical Science and Engineering,Tongji University.
文摘Intervening in the microbial environment holds promise for enhancing antitumor efficacy by reshaping the tumor microenvironment,yet few strategies have been reported.In a study led by Zou and coworkers,oral hydrogels are introduced to regulate the microbiota balance in the intestines and tumors,triggering an antitumor immune response.This work presents a microbiota-targeted drug delivery system that demonstrates notable efficacy in colon targeting and colon retention for the treatment of colorectal cancer.This represents a significant clinical advancement in treating colorectal cancer,which is particularly vulnerable to microbial infitration.
基金supported by the Natural Science Foundation of Guangdong Province(2023A1515030291)Science and Technology Program of Guangzhou(202201011130280065)Dongguan Science and Technology of Social Development Program(20211800905282).
文摘The intestinal and intratumoral microbiota are closely associated with tumor progression and response to antitumor treatments.The antibacterial or tumor microenvironment(TME)-modulating approaches have been shown to markedly improve antitumor efficacy,strategies focused on normalizing the microbial environment are rarely reported.Here,we reported the development of an orally administered inulin-based hydrogel with colon-targeting and retention effects,containing hollow MnO^(2) nanocarrier loaded with the chemotherapeutic drug Oxa(Oxa@HMI).On the one hand,beneficial bacteria in the colon specifically metabolized Oxa@HMI,resulting in the degradation of inulin and the generation of short-chain fatty acids(SCFAs).These SCFAs play a crucial role in modulating microbiota and stimulating immune responses.On the other hand,the hydrogel matrix underwent colon microbiota-specific degradation,enabling the targeted release of Oxa and production of reactive oxygen species in the acidic TME.In this study,we have established,for the first time,a microbiota-targeted drug delivery system Oxa@HMI that exhibited high efficiency in colorectal cancer targeting and colon retention.Oxa@HMI promoted chemotherapy efficiency and activated antitumor immune responses by intervening in the microbial environment within the tumor tissue,providing a crucial clinical approach for the treatment of colorectal cancer that susceptible to microbial invasion.
