Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in ...Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in the food industry.Systems metabolic engineering and synthetic biology engineering generally are believed as the comprehensive engineering approaches to obtain rationally designed strains and construct high-performance platforms for amino acids.The strate-gies focus on microbial chassis characterization optimization,precise metabolic engineering such as promoter engineer-ing,modular pathway engineering,transporter engineering,and dynamic switch systems application,and global genome streamline engineering to reduce cell burden.In this review,we summarized the efficient engineering strategies to optimize Corynebacterium glutamicum and Escherichia coli cell factories for improving the production of l-glutamate,l-lysine,l-threonine,and l-tryptophan.展开更多
Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health,medicine,agricultural,veterinary,and ...Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health,medicine,agricultural,veterinary,and food industries.The two primary strategies for constructing a microbial chassis are the top-down approach(genome reduction)and the bottom-up approach(genome synthesis).Research programs on this topic have been funded in several countries.The‘Minimum genome factory’(MGF)project was launched in 2001 in Japan with the goal of constructing microorganisms with smaller genomes for industrial use.One of the best examples of the results of this project is E.coli MGF-01,which has a reduced-genome size and exhibits better growth and higher threonine production characteristics than the parental strain[1].The‘cell factory’project was carried out from 1998 to 2002 in the Fifth Framework Program of the EU(European Union),which tried to comprehensively understand microorganisms used in the application field.One of the outstanding results of this project was the elucidation of proteins secreted by Bacillus subtilis,which was summarized as the‘secretome’[2].The GTL(Genomes to Life)program began in 2002 in the United States.In this program,researchers aimed to create artificial cells both in silico and in vitro,such as the successful design and synthesis of a minimal bacterial genome by John Craig Venter's group[3].This review provides an update on recent advances in engineering,modification and application of synthetic microbial chassis,with particular emphasis on the value of learning about chassis as a way to better understand life and improve applications.展开更多
基金supported by the National Natural Science Foundation of China(32100023)the Provincial Natural Science Foundation of Jiangsu Province(BK20210466)+2 种基金the National Natural Science Foundation of China(32000020)the Provincial Natural Science Foundation of Jiangsu Province(BK20200615)and the Youth Fund for Basic Research Program of Jiangnan University(JUSRP122009).
文摘Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in the food industry.Systems metabolic engineering and synthetic biology engineering generally are believed as the comprehensive engineering approaches to obtain rationally designed strains and construct high-performance platforms for amino acids.The strate-gies focus on microbial chassis characterization optimization,precise metabolic engineering such as promoter engineer-ing,modular pathway engineering,transporter engineering,and dynamic switch systems application,and global genome streamline engineering to reduce cell burden.In this review,we summarized the efficient engineering strategies to optimize Corynebacterium glutamicum and Escherichia coli cell factories for improving the production of l-glutamate,l-lysine,l-threonine,and l-tryptophan.
基金the National Natural Science Foundation of China(31520103902,31720103906,31670072,and 31670086)This work was also supported by the Natural Science Foundation of Hubei Province(Grant No.2016CFB257).
文摘Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health,medicine,agricultural,veterinary,and food industries.The two primary strategies for constructing a microbial chassis are the top-down approach(genome reduction)and the bottom-up approach(genome synthesis).Research programs on this topic have been funded in several countries.The‘Minimum genome factory’(MGF)project was launched in 2001 in Japan with the goal of constructing microorganisms with smaller genomes for industrial use.One of the best examples of the results of this project is E.coli MGF-01,which has a reduced-genome size and exhibits better growth and higher threonine production characteristics than the parental strain[1].The‘cell factory’project was carried out from 1998 to 2002 in the Fifth Framework Program of the EU(European Union),which tried to comprehensively understand microorganisms used in the application field.One of the outstanding results of this project was the elucidation of proteins secreted by Bacillus subtilis,which was summarized as the‘secretome’[2].The GTL(Genomes to Life)program began in 2002 in the United States.In this program,researchers aimed to create artificial cells both in silico and in vitro,such as the successful design and synthesis of a minimal bacterial genome by John Craig Venter's group[3].This review provides an update on recent advances in engineering,modification and application of synthetic microbial chassis,with particular emphasis on the value of learning about chassis as a way to better understand life and improve applications.
