Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in ...Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in the food industry.Systems metabolic engineering and synthetic biology engineering generally are believed as the comprehensive engineering approaches to obtain rationally designed strains and construct high-performance platforms for amino acids.The strate-gies focus on microbial chassis characterization optimization,precise metabolic engineering such as promoter engineer-ing,modular pathway engineering,transporter engineering,and dynamic switch systems application,and global genome streamline engineering to reduce cell burden.In this review,we summarized the efficient engineering strategies to optimize Corynebacterium glutamicum and Escherichia coli cell factories for improving the production of l-glutamate,l-lysine,l-threonine,and l-tryptophan.展开更多
Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health,medicine,agricultural,veterinary,and ...Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health,medicine,agricultural,veterinary,and food industries.The two primary strategies for constructing a microbial chassis are the top-down approach(genome reduction)and the bottom-up approach(genome synthesis).Research programs on this topic have been funded in several countries.The‘Minimum genome factory’(MGF)project was launched in 2001 in Japan with the goal of constructing microorganisms with smaller genomes for industrial use.One of the best examples of the results of this project is E.coli MGF-01,which has a reduced-genome size and exhibits better growth and higher threonine production characteristics than the parental strain[1].The‘cell factory’project was carried out from 1998 to 2002 in the Fifth Framework Program of the EU(European Union),which tried to comprehensively understand microorganisms used in the application field.One of the outstanding results of this project was the elucidation of proteins secreted by Bacillus subtilis,which was summarized as the‘secretome’[2].The GTL(Genomes to Life)program began in 2002 in the United States.In this program,researchers aimed to create artificial cells both in silico and in vitro,such as the successful design and synthesis of a minimal bacterial genome by John Craig Venter's group[3].This review provides an update on recent advances in engineering,modification and application of synthetic microbial chassis,with particular emphasis on the value of learning about chassis as a way to better understand life and improve applications.展开更多
The increasing industrial demand for high-performance materials has exposed critical limitations in conventional manufacturing approaches,particularly regarding energy efficiency and environmental sustainability.Synth...The increasing industrial demand for high-performance materials has exposed critical limitations in conventional manufacturing approaches,particularly regarding energy efficiency and environmental sustainability.Synthetic biology has emerged as a transformative solution to address these challenges by engineering biological systems,specifically via precision engineering of microbial chassis,systematic optimization of metabolic pathways,and rational redesign of enzymatic machinery.This review systematically summarizes how these approaches have driven breakthroughs across material categories.For inorganic materials,engineered biomineralization systems combining protein display technologies have achieved exceptional metal recovery efficiencies while generating functional composites with self-repairing properties.Moreover,synthetic biology tools,including chassis design,enzyme engineering,and pathway optimization have significantly advanced both the efficiency and fidelity production of proteins and nucleic acids for multifunctional material applications.Notably,the development of optimized enzyme cascades and evolved synthase variants has produced sustainable biopolymers with enhanced thermal stability,greatly promoting industrial applications.Finally,this review assesses persistent challenges and emerging research directions enabled by the integration of synthetic biology,artificial intelligence,and automation.This synergistic integration drives the development of nextgeneration circular bioeconomy frameworks from sustainable raw material processing to advanced applications.展开更多
基金supported by the National Natural Science Foundation of China(32100023)the Provincial Natural Science Foundation of Jiangsu Province(BK20210466)+2 种基金the National Natural Science Foundation of China(32000020)the Provincial Natural Science Foundation of Jiangsu Province(BK20200615)and the Youth Fund for Basic Research Program of Jiangnan University(JUSRP122009).
文摘Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in the food industry.Systems metabolic engineering and synthetic biology engineering generally are believed as the comprehensive engineering approaches to obtain rationally designed strains and construct high-performance platforms for amino acids.The strate-gies focus on microbial chassis characterization optimization,precise metabolic engineering such as promoter engineer-ing,modular pathway engineering,transporter engineering,and dynamic switch systems application,and global genome streamline engineering to reduce cell burden.In this review,we summarized the efficient engineering strategies to optimize Corynebacterium glutamicum and Escherichia coli cell factories for improving the production of l-glutamate,l-lysine,l-threonine,and l-tryptophan.
基金the National Natural Science Foundation of China(31520103902,31720103906,31670072,and 31670086)This work was also supported by the Natural Science Foundation of Hubei Province(Grant No.2016CFB257).
文摘Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health,medicine,agricultural,veterinary,and food industries.The two primary strategies for constructing a microbial chassis are the top-down approach(genome reduction)and the bottom-up approach(genome synthesis).Research programs on this topic have been funded in several countries.The‘Minimum genome factory’(MGF)project was launched in 2001 in Japan with the goal of constructing microorganisms with smaller genomes for industrial use.One of the best examples of the results of this project is E.coli MGF-01,which has a reduced-genome size and exhibits better growth and higher threonine production characteristics than the parental strain[1].The‘cell factory’project was carried out from 1998 to 2002 in the Fifth Framework Program of the EU(European Union),which tried to comprehensively understand microorganisms used in the application field.One of the outstanding results of this project was the elucidation of proteins secreted by Bacillus subtilis,which was summarized as the‘secretome’[2].The GTL(Genomes to Life)program began in 2002 in the United States.In this program,researchers aimed to create artificial cells both in silico and in vitro,such as the successful design and synthesis of a minimal bacterial genome by John Craig Venter's group[3].This review provides an update on recent advances in engineering,modification and application of synthetic microbial chassis,with particular emphasis on the value of learning about chassis as a way to better understand life and improve applications.
基金supported by the National Key R&D Program of China(grant no.2024YFA0919300 for K.L.)the National Nature Science Foundation of China(grant nos.22020102003 and 22388101 for H.J.Z.,22125701 for K.L.,52222214 and 52372274 for F.W.,22422704 and 22377121 for J.J.L.,52472112 and 22207104 for Y.W.L.,T2322025 and 82272161 for J.J.S.,and 22407071 for H.J.C.)+3 种基金the China Postdoctoral Science Foundation(grant no.2024M753180 for Y.Y.L.)the Natural Science Foundation of Jilin Province,China(grant no.20240101175JC for F.W.)the Xiangfu Lab Research Project(grant no.XF012022C0200 for K.L.)the National High Level Hospital Clinical Research Funding and Fundamental Research Funds for the Central Universities(grant no.BJ-2023-118 for J.J.S.).
文摘The increasing industrial demand for high-performance materials has exposed critical limitations in conventional manufacturing approaches,particularly regarding energy efficiency and environmental sustainability.Synthetic biology has emerged as a transformative solution to address these challenges by engineering biological systems,specifically via precision engineering of microbial chassis,systematic optimization of metabolic pathways,and rational redesign of enzymatic machinery.This review systematically summarizes how these approaches have driven breakthroughs across material categories.For inorganic materials,engineered biomineralization systems combining protein display technologies have achieved exceptional metal recovery efficiencies while generating functional composites with self-repairing properties.Moreover,synthetic biology tools,including chassis design,enzyme engineering,and pathway optimization have significantly advanced both the efficiency and fidelity production of proteins and nucleic acids for multifunctional material applications.Notably,the development of optimized enzyme cascades and evolved synthase variants has produced sustainable biopolymers with enhanced thermal stability,greatly promoting industrial applications.Finally,this review assesses persistent challenges and emerging research directions enabled by the integration of synthetic biology,artificial intelligence,and automation.This synergistic integration drives the development of nextgeneration circular bioeconomy frameworks from sustainable raw material processing to advanced applications.
