Microbial corrosion of hydraulic concrete structures(HCSs)has received increasing research concerns.However,knowledge on the morphology of attached biofilms,as well as the community structures and functions cultivated...Microbial corrosion of hydraulic concrete structures(HCSs)has received increasing research concerns.However,knowledge on the morphology of attached biofilms,as well as the community structures and functions cultivated under variable nutrient levels is lacking.Here,biofilm colonization patterns and community structures responding to variable levels of ammonia and sulfate were explored.From field sampling,NH_(4)^(+)-N was proven key factor governing community structure in attached biofilms,verifying the reliability of selecting target nutrient species in batch experiments.Biofilms exhibited significant compositional differences in field sampling and incubation experiments.As the nutrient increased in batch experiments,the growth of biofilms gradually slowed down and uneven distribution was detected.The proportions of proteins and β-d-glucose polysaccharides in biofilms experienced a decrease in response to elevated levels of nutrients.With the increased of nutrients,themass losses of concretes exhibited an increase,reaching a highest value of 2.37%in the presence of 20 mg/L of ammonia.Microbial communities underwent a significant transition in structure and metabolic functions to ammonia gradient.The highest activity of nitrification was observed in biofilms colonized in the presence of 20 mg/L of ammonia.While the communities and their functions remained relativelymore stable responding to sulfate gradient.Our research provides novel insights into the structures of biofilms attached on HCSs and the metabolic functions in the presence of high level of nutrients,which is of significance for the operation and maintenance of hydraulic engineering structures.展开更多
Using the concept of the interactive water rock- microbe system of ocean floor, the microbialmineralization of ocean-floor polymetallic concretions in the central Pacific Ocean has been studied for the firsttime. Thro...Using the concept of the interactive water rock- microbe system of ocean floor, the microbialmineralization of ocean-floor polymetallic concretions in the central Pacific Ocean has been studied for the firsttime. Through the correlation and study of the microbial activity and formation mechanism of polymetallicconcretions, the microbial and chemical processes for. transforming mineralizing materials and the observationand determination of the concentration of mineralizing material in the system and the variation ofenvironmental parameters, this paper reveals the reaction rate and evolutionary direction of mineralizing com-ponents caused by microbial activity, expounds the microbial mineralization mechanism and formation modelof polymetallic concretions from the angle of microbial geochemical action, and suggests a threefold division ofthe microbial mineralization stages展开更多
基金supported by the National Key Research and Development Project of China(No.2021YFB2600200)the National Natural Science Foundation of China(Nos.52470185 and 52170159)the Open Research Fund of National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety,the Fund of National Key Laboratory of Water Disaster Prevention and Key Research and Development Program of Jiangsu Province(No.BE2022601).
文摘Microbial corrosion of hydraulic concrete structures(HCSs)has received increasing research concerns.However,knowledge on the morphology of attached biofilms,as well as the community structures and functions cultivated under variable nutrient levels is lacking.Here,biofilm colonization patterns and community structures responding to variable levels of ammonia and sulfate were explored.From field sampling,NH_(4)^(+)-N was proven key factor governing community structure in attached biofilms,verifying the reliability of selecting target nutrient species in batch experiments.Biofilms exhibited significant compositional differences in field sampling and incubation experiments.As the nutrient increased in batch experiments,the growth of biofilms gradually slowed down and uneven distribution was detected.The proportions of proteins and β-d-glucose polysaccharides in biofilms experienced a decrease in response to elevated levels of nutrients.With the increased of nutrients,themass losses of concretes exhibited an increase,reaching a highest value of 2.37%in the presence of 20 mg/L of ammonia.Microbial communities underwent a significant transition in structure and metabolic functions to ammonia gradient.The highest activity of nitrification was observed in biofilms colonized in the presence of 20 mg/L of ammonia.While the communities and their functions remained relativelymore stable responding to sulfate gradient.Our research provides novel insights into the structures of biofilms attached on HCSs and the metabolic functions in the presence of high level of nutrients,which is of significance for the operation and maintenance of hydraulic engineering structures.
文摘Using the concept of the interactive water rock- microbe system of ocean floor, the microbialmineralization of ocean-floor polymetallic concretions in the central Pacific Ocean has been studied for the firsttime. Through the correlation and study of the microbial activity and formation mechanism of polymetallicconcretions, the microbial and chemical processes for. transforming mineralizing materials and the observationand determination of the concentration of mineralizing material in the system and the variation ofenvironmental parameters, this paper reveals the reaction rate and evolutionary direction of mineralizing com-ponents caused by microbial activity, expounds the microbial mineralization mechanism and formation modelof polymetallic concretions from the angle of microbial geochemical action, and suggests a threefold division ofthe microbial mineralization stages
