Colorectal cancer remains one of the leading causes of morbidity and mortality worldwide.Despite notable advances in early detection and therapeutic strategies,the molecular mechanisms underlying tumor survival,chemot...Colorectal cancer remains one of the leading causes of morbidity and mortality worldwide.Despite notable advances in early detection and therapeutic strategies,the molecular mechanisms underlying tumor survival,chemotherapy resistance,and metastasis are not yet fully understood.MicroRNAs(miRNAs)have emerged as pivotal regulators of cancer development,as they modulate gene expression and orchestrate key signaling pathways.However,the epigenetic mechanisms that control miRNA expression and their downstream gene targets remain largely unclear.In this review,we highlight the critical role of the colorectal cancer microenvironment in influencing miRNA expression and discuss how this regulation contributes to tumorigenesis.A better understanding of these processes may lead to the identification of novel therapeutic targets and strategies to prevent recurrence.展开更多
The published article titled“MicroRNA-133b Inhibits Proliferation,Cellular Migration,and Invasion via Targeting LASP1 in Hepatocarcinoma Cells”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1269–1282.
The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3...The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3727/096504017X14850134190255 URL:https://www.techscience.com/or/v25n8/56908 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
目的观察缺血性脑损伤后大鼠缺血皮质区microRNA 210的表达变化规律,探讨mir-210对脑缺血后血管新生的可能影响。方法实验分为缺血后1d、3d、7d组和假手术组(n=5),缺血组采用线栓法制作大脑中动脉阻塞模型,于缺血后1、3、7d处死大鼠,取...目的观察缺血性脑损伤后大鼠缺血皮质区microRNA 210的表达变化规律,探讨mir-210对脑缺血后血管新生的可能影响。方法实验分为缺血后1d、3d、7d组和假手术组(n=5),缺血组采用线栓法制作大脑中动脉阻塞模型,于缺血后1、3、7d处死大鼠,取缺血皮质区用于实验;假手术组仅暴露动脉。提取各实验组脑缺血皮质区的miRNA,Real time PCR比较各实验组mir-210的表达水平。结果脑缺血后mir-210表达显著上调,缺血后1d、3d、7d分别为假手术组的7.2±0.56、20.1±0.87和20.3±0.76倍(P<0.05)。结论脑缺血后mir-210表达显著上调,mir-210可能在促进脑缺血后血管新生过程中起重要作用。展开更多
目的:检测MicroRNA-210(miR-210)在儿童急性淋巴细胞白血病(ALL)中的表达水平,探讨miR-210与微小残留病灶(MRD)联合检测对指导临床治疗及判断预后的意义。方法:采用RQ-PCR方法检测88例初诊儿童ALL骨髓样本中miR-210的表达水平及第33天的...目的:检测MicroRNA-210(miR-210)在儿童急性淋巴细胞白血病(ALL)中的表达水平,探讨miR-210与微小残留病灶(MRD)联合检测对指导临床治疗及判断预后的意义。方法:采用RQ-PCR方法检测88例初诊儿童ALL骨髓样本中miR-210的表达水平及第33天的MRD表达水平。结果:miR-210在儿童ALL初诊骨髓样本中普遍高表达,且在非复发组miR-210的表达水平显著高于复发组(10.64±1.5 vs 3.27±0.68)(P<0.05),KaplanMeier生存分析结果表明,与高表达组相比,miR-210低表达组具有较差的无复发活存率(RFS)(P=0.011)、无事件活存率(EFS)(P=0.013)及总活存率(OS)(P=0.0108)。根据miR-210及MRD水平,将88例患儿分为3组。miR-210-MRD高风险组复发率(70%)显著高于miR-210-MRD中风险组(6.25%)及miR-210-MRD低风险组(2.1%)。Kaplan-Meier生存分析结果表明,miR-210-MRD高风险组RFS、EFS及OS均显著低于中风险组和低风险组(P<0.01)。结论:儿童ALL初诊骨髓样本中miR-210的表达水平对疾病复发、诱导失败有良好的预测价值。miR-210的低表达与低LFS、EFS及OS高度相关。miR-210与第33天MRD水平的联合检测,可以更准确地识别出预后差、复发风险大的患儿。展开更多
文摘Colorectal cancer remains one of the leading causes of morbidity and mortality worldwide.Despite notable advances in early detection and therapeutic strategies,the molecular mechanisms underlying tumor survival,chemotherapy resistance,and metastasis are not yet fully understood.MicroRNAs(miRNAs)have emerged as pivotal regulators of cancer development,as they modulate gene expression and orchestrate key signaling pathways.However,the epigenetic mechanisms that control miRNA expression and their downstream gene targets remain largely unclear.In this review,we highlight the critical role of the colorectal cancer microenvironment in influencing miRNA expression and discuss how this regulation contributes to tumorigenesis.A better understanding of these processes may lead to the identification of novel therapeutic targets and strategies to prevent recurrence.
文摘The published article titled“MicroRNA-133b Inhibits Proliferation,Cellular Migration,and Invasion via Targeting LASP1 in Hepatocarcinoma Cells”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1269–1282.
文摘The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3727/096504017X14850134190255 URL:https://www.techscience.com/or/v25n8/56908 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘目的观察缺血性脑损伤后大鼠缺血皮质区microRNA 210的表达变化规律,探讨mir-210对脑缺血后血管新生的可能影响。方法实验分为缺血后1d、3d、7d组和假手术组(n=5),缺血组采用线栓法制作大脑中动脉阻塞模型,于缺血后1、3、7d处死大鼠,取缺血皮质区用于实验;假手术组仅暴露动脉。提取各实验组脑缺血皮质区的miRNA,Real time PCR比较各实验组mir-210的表达水平。结果脑缺血后mir-210表达显著上调,缺血后1d、3d、7d分别为假手术组的7.2±0.56、20.1±0.87和20.3±0.76倍(P<0.05)。结论脑缺血后mir-210表达显著上调,mir-210可能在促进脑缺血后血管新生过程中起重要作用。
文摘目的:检测MicroRNA-210(miR-210)在儿童急性淋巴细胞白血病(ALL)中的表达水平,探讨miR-210与微小残留病灶(MRD)联合检测对指导临床治疗及判断预后的意义。方法:采用RQ-PCR方法检测88例初诊儿童ALL骨髓样本中miR-210的表达水平及第33天的MRD表达水平。结果:miR-210在儿童ALL初诊骨髓样本中普遍高表达,且在非复发组miR-210的表达水平显著高于复发组(10.64±1.5 vs 3.27±0.68)(P<0.05),KaplanMeier生存分析结果表明,与高表达组相比,miR-210低表达组具有较差的无复发活存率(RFS)(P=0.011)、无事件活存率(EFS)(P=0.013)及总活存率(OS)(P=0.0108)。根据miR-210及MRD水平,将88例患儿分为3组。miR-210-MRD高风险组复发率(70%)显著高于miR-210-MRD中风险组(6.25%)及miR-210-MRD低风险组(2.1%)。Kaplan-Meier生存分析结果表明,miR-210-MRD高风险组RFS、EFS及OS均显著低于中风险组和低风险组(P<0.01)。结论:儿童ALL初诊骨髓样本中miR-210的表达水平对疾病复发、诱导失败有良好的预测价值。miR-210的低表达与低LFS、EFS及OS高度相关。miR-210与第33天MRD水平的联合检测,可以更准确地识别出预后差、复发风险大的患儿。
