目的分析microRNA-30a(miR-30a)在慢性肾衰竭患者透析前后血清中的表达差异,评价其在评估慢性肾衰竭血液透析效果中的作用。方法选取2012年10月至2013年12月本科收治的慢性肾衰竭患者40例,分别在患者透析前后采血并获得血清样本,采用mir...目的分析microRNA-30a(miR-30a)在慢性肾衰竭患者透析前后血清中的表达差异,评价其在评估慢性肾衰竭血液透析效果中的作用。方法选取2012年10月至2013年12月本科收治的慢性肾衰竭患者40例,分别在患者透析前后采血并获得血清样本,采用mirV ana PARIS Kit提取血清样本中总RNA,利用qRT-PCR法检测miR-30a的表达,临床生化检测患者透析前后血清中肌酐(Cr)、尿素氮(Ur)及β2微球蛋白(β2-MG)的含量,表达值行曼-惠特尼U检验,工作特征曲线(ROC curve)评估miR-30a在慢性肾衰竭患者透析效果中的价值。结果 miR-30a、Ur和β2-MG在慢性肾衰竭患者透析后的表达显著低于透析前(P<0.05,P<0.01),ROC curve显示在患者透析后miR-30a的表达低于透析前0.25倍以上时患者可获得较好的透析效果。结论 miR-30a可作为一种潜在的评估慢性肾衰竭血液透析效果的生物标志物。展开更多
Background Acute kidney injury(AKI)is a primary feature of renal complications in patients with sepsis.MicroRNA(miRNA/miR)-30a is an essential regulator of cardiovascular diseases,tumors,phagocytosis,and other physica...Background Acute kidney injury(AKI)is a primary feature of renal complications in patients with sepsis.MicroRNA(miRNA/miR)-30a is an essential regulator of cardiovascular diseases,tumors,phagocytosis,and other physical processes,but whether it participates in sepsis-induced AKI(sepsis-AKI)is unknown.We aimed to elucidate the functions and molecular mechanism underlying miR-30a activity in sepsis-AKI.Methods The classical cecal ligation and puncture(CLP)method and lipopolysaccharide(LPS)-induced Human Kidney 2(HK-2)cells were used to establish in vivo and in vitro sepsis-AKI models.Specific pathogen-free and mature male Sprague-Dawley(SD)rats,aged 6–8 weeks(weight 200–250 g),were randomly divided into five-time phase subgroups.Fluid resuscitation with 30 mL/kg 37°C saline was administered after the operation,without antibiotics.Formalin-fixed,paraffin-embedded kidney sections were stained with hematoxylin and eosin.SD rat kidney tissue samples were collected for analysis by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay.HK-2 cells were transfected with hsa-miR-30a-3p mimics or inhibitors,and compared with untreated normal controls.RNA,protein,and cell viability were evaluated by quantitative reverse transcription-polymerase chain reaction(qRT-PCR),western blot,and cell counting kit-8 methods.A Dual-Luciferase Assay Kit(Promega)was used to measure luciferase activity 48 h after transfection with miR-30a-3p mimics.Results Expression levels of miR-30a-3p and miR-30a-5p in renal tissues of the sepsis group were significantly reduced at 12 h and 24 h(P<0.05).Tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were significantly increased in renal tissue 3 h after the operation in rats(P<0.05),and gradually decreased 6 h,12 h,and 24 h after CLP.Levels of miR-30a-5p and miR-30a-3p were significantly down-regulated at 3 h after LPS treatment(P<0.05),and gradually decreased in HK-2 cells.One hour after LPS(10µg/mL)treatment,TNF-αand IL-1βlevels in HK-2 cells were significantly up-regulated(P<0.05),and they were markedly down-regulated after 3 h(P<0.05).IL-6 expression levels began to rise after LPS treatment of cells,peaked at 6 h(P<0.05),and then decreased to the initial level within a few hours.Stimulation with 10µg/mL LPS promoted HK-2 cells proliferation,which was inhibited after miR-30a-3p-mimic transfection.Bioinformatics prediction identified 37 potential miR-30a-3p target genes,including transcriptional enhanced associate domain 1(TEAD1).After transfection of HK-2 cells with miR-30a-3p mimics and miR-30a-3p inhibitor,TEAD1 transcript was significantly up-and down-regulated,respectively(both P<0.05).After LPS treatment(24 h),expression of TEAD1 in the inhibitors group was significantly increased(P<0.01),while that in the mimics group was significantly suppressed(P<0.01).In the dual luciferase reporter experiment,miR-30a-3p overexpression decreased fluorescence intensity(P<0.01)from TEAD1-wt-containing plasmids,but did not influence fluorescence intensity from TEAD1-muta-containing plasmids.LPS may promote HK-2 cells proliferation through the miR-30a-3p/TEAD1 pathway.Conclusion In a background of expression of inflammatory factors,including TNF-α,IL-1β,and IL-6,which were transiently increased in the sepsis-AKI model,miR-30a was down-regulated.Down-regulated miR-30a-3p may promote cell proliferation by targeting TEAD1 in LPS-induced HK-2 cells,demonstrating its potential as a biomarker for early sepsis-AKI diagnosis.展开更多
国际原子能机构(International Atomic Energy Agency,简称IAEA)发布的《Safety Classification of Structures,Systems and Components in Nuclear Power Plants》(No.SSG-30)从安全功能分类和设计预防措施两个维度出发,结合失效后果...国际原子能机构(International Atomic Energy Agency,简称IAEA)发布的《Safety Classification of Structures,Systems and Components in Nuclear Power Plants》(No.SSG-30)从安全功能分类和设计预防措施两个维度出发,结合失效后果严重程度、物项类型等因素,为核电厂构筑物、系统和部件(SSCs)的安全分级提供了具有可操作性的方法论框架。本文基于SSG-30,对核电饱和式蒸汽发生器主要零部件的安全分级进行了研究,对其承压边界、内部构件及密封组件等关键部件进行了安全分级探讨,并根据防振条组件和套筒支承板的安全分级开展了力学评估分析。经分析,基于SSG-30的核电蒸汽发生器分级可为核电设备分级和力学评估提供参考,对于核电机组的安全运行水平具有重要的意义。展开更多
土壤侵蚀是影响全球土地退化和可持续发展的重大环境问题。土壤侵蚀模型可估算土壤流失的空间和时间分布,为制定流域水土资源保护政策与实施有效战略提供支持。修订版通用土壤流失方程(RUSLE,Revised Universal Soil Loss Equation)模...土壤侵蚀是影响全球土地退化和可持续发展的重大环境问题。土壤侵蚀模型可估算土壤流失的空间和时间分布,为制定流域水土资源保护政策与实施有效战略提供支持。修订版通用土壤流失方程(RUSLE,Revised Universal Soil Loss Equation)模型以其兼具简单性与准确性,成为全球应用最广泛的土壤侵蚀模型之一。在RUSLE模型的输入参数中,地形因子(LS因子)对土壤流失潜力的影响最为显著,而LS因子的输入数据和计算方法对最终RUSLE模型计算的质量会有直接影响。为此构建了中国区域30米分辨率LS因子数据集(LS_China)。本数据集采用开源工具SAGA(自动化地球科学分析系统)和GDAL(地理空间数据抽象库),基于公开的SRTM30米高程数据集(SRTMGL1)计算获得,覆盖整个中国区域。数据被组织成单独的栅格化图块,每个栅格化图块覆盖1°×1°的范围,以Geotiff格式存储。在大尺度范围的数据计算过程中,基于对数据的空间分解,采用邻域依赖的计算方法,同时采用多流算法,保证LS因子计算的准确性。在数据质量控制方面,通过变异系数(CV)验证并与同一区域的其他数据集进行比较。结果表明,LS_China数据集的变异系数为1.24,相较于其他数据集,其内部异质性较小,具备高质量和高可靠性。本数据集可用作各种尺度(地方、区域、国家)的任何土壤侵蚀评估的输入数据。展开更多
文摘目的分析microRNA-30a(miR-30a)在慢性肾衰竭患者透析前后血清中的表达差异,评价其在评估慢性肾衰竭血液透析效果中的作用。方法选取2012年10月至2013年12月本科收治的慢性肾衰竭患者40例,分别在患者透析前后采血并获得血清样本,采用mirV ana PARIS Kit提取血清样本中总RNA,利用qRT-PCR法检测miR-30a的表达,临床生化检测患者透析前后血清中肌酐(Cr)、尿素氮(Ur)及β2微球蛋白(β2-MG)的含量,表达值行曼-惠特尼U检验,工作特征曲线(ROC curve)评估miR-30a在慢性肾衰竭患者透析效果中的价值。结果 miR-30a、Ur和β2-MG在慢性肾衰竭患者透析后的表达显著低于透析前(P<0.05,P<0.01),ROC curve显示在患者透析后miR-30a的表达低于透析前0.25倍以上时患者可获得较好的透析效果。结论 miR-30a可作为一种潜在的评估慢性肾衰竭血液透析效果的生物标志物。
文摘Background Acute kidney injury(AKI)is a primary feature of renal complications in patients with sepsis.MicroRNA(miRNA/miR)-30a is an essential regulator of cardiovascular diseases,tumors,phagocytosis,and other physical processes,but whether it participates in sepsis-induced AKI(sepsis-AKI)is unknown.We aimed to elucidate the functions and molecular mechanism underlying miR-30a activity in sepsis-AKI.Methods The classical cecal ligation and puncture(CLP)method and lipopolysaccharide(LPS)-induced Human Kidney 2(HK-2)cells were used to establish in vivo and in vitro sepsis-AKI models.Specific pathogen-free and mature male Sprague-Dawley(SD)rats,aged 6–8 weeks(weight 200–250 g),were randomly divided into five-time phase subgroups.Fluid resuscitation with 30 mL/kg 37°C saline was administered after the operation,without antibiotics.Formalin-fixed,paraffin-embedded kidney sections were stained with hematoxylin and eosin.SD rat kidney tissue samples were collected for analysis by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay.HK-2 cells were transfected with hsa-miR-30a-3p mimics or inhibitors,and compared with untreated normal controls.RNA,protein,and cell viability were evaluated by quantitative reverse transcription-polymerase chain reaction(qRT-PCR),western blot,and cell counting kit-8 methods.A Dual-Luciferase Assay Kit(Promega)was used to measure luciferase activity 48 h after transfection with miR-30a-3p mimics.Results Expression levels of miR-30a-3p and miR-30a-5p in renal tissues of the sepsis group were significantly reduced at 12 h and 24 h(P<0.05).Tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were significantly increased in renal tissue 3 h after the operation in rats(P<0.05),and gradually decreased 6 h,12 h,and 24 h after CLP.Levels of miR-30a-5p and miR-30a-3p were significantly down-regulated at 3 h after LPS treatment(P<0.05),and gradually decreased in HK-2 cells.One hour after LPS(10µg/mL)treatment,TNF-αand IL-1βlevels in HK-2 cells were significantly up-regulated(P<0.05),and they were markedly down-regulated after 3 h(P<0.05).IL-6 expression levels began to rise after LPS treatment of cells,peaked at 6 h(P<0.05),and then decreased to the initial level within a few hours.Stimulation with 10µg/mL LPS promoted HK-2 cells proliferation,which was inhibited after miR-30a-3p-mimic transfection.Bioinformatics prediction identified 37 potential miR-30a-3p target genes,including transcriptional enhanced associate domain 1(TEAD1).After transfection of HK-2 cells with miR-30a-3p mimics and miR-30a-3p inhibitor,TEAD1 transcript was significantly up-and down-regulated,respectively(both P<0.05).After LPS treatment(24 h),expression of TEAD1 in the inhibitors group was significantly increased(P<0.01),while that in the mimics group was significantly suppressed(P<0.01).In the dual luciferase reporter experiment,miR-30a-3p overexpression decreased fluorescence intensity(P<0.01)from TEAD1-wt-containing plasmids,but did not influence fluorescence intensity from TEAD1-muta-containing plasmids.LPS may promote HK-2 cells proliferation through the miR-30a-3p/TEAD1 pathway.Conclusion In a background of expression of inflammatory factors,including TNF-α,IL-1β,and IL-6,which were transiently increased in the sepsis-AKI model,miR-30a was down-regulated.Down-regulated miR-30a-3p may promote cell proliferation by targeting TEAD1 in LPS-induced HK-2 cells,demonstrating its potential as a biomarker for early sepsis-AKI diagnosis.
文摘国际原子能机构(International Atomic Energy Agency,简称IAEA)发布的《Safety Classification of Structures,Systems and Components in Nuclear Power Plants》(No.SSG-30)从安全功能分类和设计预防措施两个维度出发,结合失效后果严重程度、物项类型等因素,为核电厂构筑物、系统和部件(SSCs)的安全分级提供了具有可操作性的方法论框架。本文基于SSG-30,对核电饱和式蒸汽发生器主要零部件的安全分级进行了研究,对其承压边界、内部构件及密封组件等关键部件进行了安全分级探讨,并根据防振条组件和套筒支承板的安全分级开展了力学评估分析。经分析,基于SSG-30的核电蒸汽发生器分级可为核电设备分级和力学评估提供参考,对于核电机组的安全运行水平具有重要的意义。
文摘土壤侵蚀是影响全球土地退化和可持续发展的重大环境问题。土壤侵蚀模型可估算土壤流失的空间和时间分布,为制定流域水土资源保护政策与实施有效战略提供支持。修订版通用土壤流失方程(RUSLE,Revised Universal Soil Loss Equation)模型以其兼具简单性与准确性,成为全球应用最广泛的土壤侵蚀模型之一。在RUSLE模型的输入参数中,地形因子(LS因子)对土壤流失潜力的影响最为显著,而LS因子的输入数据和计算方法对最终RUSLE模型计算的质量会有直接影响。为此构建了中国区域30米分辨率LS因子数据集(LS_China)。本数据集采用开源工具SAGA(自动化地球科学分析系统)和GDAL(地理空间数据抽象库),基于公开的SRTM30米高程数据集(SRTMGL1)计算获得,覆盖整个中国区域。数据被组织成单独的栅格化图块,每个栅格化图块覆盖1°×1°的范围,以Geotiff格式存储。在大尺度范围的数据计算过程中,基于对数据的空间分解,采用邻域依赖的计算方法,同时采用多流算法,保证LS因子计算的准确性。在数据质量控制方面,通过变异系数(CV)验证并与同一区域的其他数据集进行比较。结果表明,LS_China数据集的变异系数为1.24,相较于其他数据集,其内部异质性较小,具备高质量和高可靠性。本数据集可用作各种尺度(地方、区域、国家)的任何土壤侵蚀评估的输入数据。
