Background:Cholangiocarcinoma(CCA)is a malignant biliary tract tumor with an extremely poor prognosis.There is an urgent demand to explore novel therapeutic strategies.L-fucose has been confirmed to participate in ant...Background:Cholangiocarcinoma(CCA)is a malignant biliary tract tumor with an extremely poor prognosis.There is an urgent demand to explore novel therapeutic strategies.L-fucose has been confirmed to participate in anti-inflammation and antitumor activities.However,the effect of L-fucose on the progression of CCA has not been well investigated.This study aimed to determine whether L-fucose induced the inhibition of CCA and its possible mechanism.Methods:The anti-growth activity was determined using Cell Counting Kit-8 assay,colony formation assays,Annexin V-fluorescein isothiocyanate/propidium iodide(FITC/PI)assay,and cell cycle analysis.The anti-metastasis activity was determined by wound healing,transwell,and invasion assays.The anti-angiogenesis activity was determined by tube formation and transwell assays.MicroRNAs that may be involved in the L-fucose-induced CCA inhibition was analyzed using bioinformatics methods.The preclinical therapeutic efficacy was mainly estimated by ultrasound in xenograft nude mouse models.Differences were analyzed via Student’s t test or one-way analysis of variance.Results:L-Fucose induced apoptosis and G0/G1 cell cycle arrest,inhibited cell epithelial-mesenchymal transition of CCA cells,and additionally inhibited tube formation of human umbilical vein endothelial cells(HUVECs)in a dose-dependent manner,leading to a decrease in cell proliferation,metastasis,and angiogenesis.Mechanistically,L-fucose induced microRNA-200b(miR-200b)upregulation,and mitogen-activated protein kinase 7(MAPK7)downregulation was found to be targeted by miR-200b,with decreased cell proliferation and metastasis.Additionally,phosphorylated signal transducer and activator of transcription 3 was found to be downregulated after L-fucose treatment.Finally,in vivo experiments in CCA xenograft models also confirmed the antitumor properties of L-fucose.Conclusion:L-Fucose inhibited the progression of CCA via the miR-200b/MAPK7 and signal transducer and activator of transcription 3 signaling pathways.展开更多
Signal Hound SM200系列接收机集高性能与高灵敏度于一体,是专业射频信号分析与监测领域的实时频谱分析设备。该系列设备凭借卓越的综合性能,广泛应用于无线电监管、频谱监测、电磁环境评估等专业领域,在复杂电磁环境实时监测、非法信...Signal Hound SM200系列接收机集高性能与高灵敏度于一体,是专业射频信号分析与监测领域的实时频谱分析设备。该系列设备凭借卓越的综合性能,广泛应用于无线电监管、频谱监测、电磁环境评估等专业领域,在复杂电磁环境实时监测、非法信号精准溯源及无线电频谱资源优化配置中发挥关键作用,为频谱管理与无线通信安全提供坚实的技术支撑。然而,该系列设备因缺乏多频段扫描频谱同步显示功能,致使多频段监测值守效率受限。基于此,本文以SM200B接收机为开发对象,通过优化频谱扫描显示机制,实现同屏多频段频谱并行显示功能,为提高无线电监测工作效率提供技术支撑。展开更多
基金National Natural Science Foundation of China(Nos.81720108006,81974062)。
文摘Background:Cholangiocarcinoma(CCA)is a malignant biliary tract tumor with an extremely poor prognosis.There is an urgent demand to explore novel therapeutic strategies.L-fucose has been confirmed to participate in anti-inflammation and antitumor activities.However,the effect of L-fucose on the progression of CCA has not been well investigated.This study aimed to determine whether L-fucose induced the inhibition of CCA and its possible mechanism.Methods:The anti-growth activity was determined using Cell Counting Kit-8 assay,colony formation assays,Annexin V-fluorescein isothiocyanate/propidium iodide(FITC/PI)assay,and cell cycle analysis.The anti-metastasis activity was determined by wound healing,transwell,and invasion assays.The anti-angiogenesis activity was determined by tube formation and transwell assays.MicroRNAs that may be involved in the L-fucose-induced CCA inhibition was analyzed using bioinformatics methods.The preclinical therapeutic efficacy was mainly estimated by ultrasound in xenograft nude mouse models.Differences were analyzed via Student’s t test or one-way analysis of variance.Results:L-Fucose induced apoptosis and G0/G1 cell cycle arrest,inhibited cell epithelial-mesenchymal transition of CCA cells,and additionally inhibited tube formation of human umbilical vein endothelial cells(HUVECs)in a dose-dependent manner,leading to a decrease in cell proliferation,metastasis,and angiogenesis.Mechanistically,L-fucose induced microRNA-200b(miR-200b)upregulation,and mitogen-activated protein kinase 7(MAPK7)downregulation was found to be targeted by miR-200b,with decreased cell proliferation and metastasis.Additionally,phosphorylated signal transducer and activator of transcription 3 was found to be downregulated after L-fucose treatment.Finally,in vivo experiments in CCA xenograft models also confirmed the antitumor properties of L-fucose.Conclusion:L-Fucose inhibited the progression of CCA via the miR-200b/MAPK7 and signal transducer and activator of transcription 3 signaling pathways.
