MiR-200a was shown to be upregulated in the corpus cavernosum （CC） of rats with aging-related erectile dysfunction （A-ED） in our previous study. Among its target genes, SIRT1 was also reported as a protective fact...MiR-200a was shown to be upregulated in the corpus cavernosum （CC） of rats with aging-related erectile dysfunction （A-ED） in our previous study. Among its target genes, SIRT1 was also reported as a protective factor in erectile function by our groups previously. Thus, miR-200a might attenuate the erectile function in A-ED via SIRT1 inhibition. In the present study, three animal groups were included： aged rats with ED （group AE, n = 8）, aged rats with normal erectile function （group AN, n = 8）, and young rats as normal controls （group YN, n = 8）. CCs from each group were collected for histological and molecular measurements to validate the dysregulation of miR-200a and SIRT1. After that, the cavernous endothelial cells （CECs） from CC of aged rats with normal erectile function were transfected with miR-200a in vitro. Then the expression of SIRT1 and molecules within the eNOS/NO/PKG pathway were measured to investigate whether the transfection could imitate the attenuated process of erectile function in the aged. As a result, miR-200a was upregulated while the SIRT1, the levels of eNOS and cGMP were all downregulated in the CCs from AE group. After transfection in vitro, the miR-200a was upregulated while the SIRT1 and levels of eNOS and cGMP were obviously downregulated. Finally, based on the results of our previous study, we further verify that up-regulation of miR-200a could participate in the mechanisms of A-ED via SIRT1 inhibition, and mainly attenuate endothelial function via influencing the eNOS/NO/PKG pathway.展开更多
microRNA(miRNA)is a type of small non-coding RNA that can participate in cell proliferation and apoptosis by regulating gene expression.More and more evidences indicate that miRNA-200a is involved in the occurrence an...microRNA(miRNA)is a type of small non-coding RNA that can participate in cell proliferation and apoptosis by regulating gene expression.More and more evidences indicate that miRNA-200a is involved in the occurrence and development of non-alcoholic fatty liver disease,alcoholic liver disease,drug-induced liver injury,liver fibrosis,and hepatocellular carcinoma.Downstream target genes of serotonin,regulating related signal pathways and playing different roles in the progression of a variety of liver diseases,provide a reference for exploring the mechanism of a variety of chronic liver diseases.展开更多
目的总结microRNA-200(miR-200)家族在三阴性乳腺癌(triple-negative breast cancer,TNBC)中的研究进展。方法系统检索并阅读国内外的相关文献,对近年来miR-200家族在TNBC中的研究进展进行综述。结果miR-200家族在TNBC的增殖、侵袭转移...目的总结microRNA-200(miR-200)家族在三阴性乳腺癌(triple-negative breast cancer,TNBC)中的研究进展。方法系统检索并阅读国内外的相关文献,对近年来miR-200家族在TNBC中的研究进展进行综述。结果miR-200家族在TNBC的增殖、侵袭转移及治疗抵抗性中发挥重要作用,并可作为潜在的治疗靶点和生物预测因子。不同的miR-200家族成员及其差异表达介导不同的靶向作用,这可能与信号通路及细胞环境差异有关。结论miR-200家族在TNBC的发生和发展过程中具有关键性的调控作用,这有望为TNBC的治疗及预后评估提供新思路,但其作用机制仍待进一步的深入研究。展开更多
基金ACKNOWLEDGMENT This work was supported by grant from the National Natural Science Foundation of China （81170563 ）, and funded by Key Proj oct supported by Medical Science and Technology development Foundation, Nanjing Department of Health （YKK12096）, and by Key Project supported by Science and Technology development Foundation, Nanjing Medical University （2014NJMUZD053）.
文摘MiR-200a was shown to be upregulated in the corpus cavernosum （CC） of rats with aging-related erectile dysfunction （A-ED） in our previous study. Among its target genes, SIRT1 was also reported as a protective factor in erectile function by our groups previously. Thus, miR-200a might attenuate the erectile function in A-ED via SIRT1 inhibition. In the present study, three animal groups were included： aged rats with ED （group AE, n = 8）, aged rats with normal erectile function （group AN, n = 8）, and young rats as normal controls （group YN, n = 8）. CCs from each group were collected for histological and molecular measurements to validate the dysregulation of miR-200a and SIRT1. After that, the cavernous endothelial cells （CECs） from CC of aged rats with normal erectile function were transfected with miR-200a in vitro. Then the expression of SIRT1 and molecules within the eNOS/NO/PKG pathway were measured to investigate whether the transfection could imitate the attenuated process of erectile function in the aged. As a result, miR-200a was upregulated while the SIRT1, the levels of eNOS and cGMP were all downregulated in the CCs from AE group. After transfection in vitro, the miR-200a was upregulated while the SIRT1 and levels of eNOS and cGMP were obviously downregulated. Finally, based on the results of our previous study, we further verify that up-regulation of miR-200a could participate in the mechanisms of A-ED via SIRT1 inhibition, and mainly attenuate endothelial function via influencing the eNOS/NO/PKG pathway.
基金National Natural Science Foundation of China(No.81860790)Guangxi Science and Technology Project(No.Guike AB20297002)+3 种基金Guangxi Natural Science Foundation(No.2020GXNSFAA297160)Guangxi Natural Science Foundation(No.2018GXNSFBA050050)Guangxi First-class Discipline Integrated Traditional Chinese and Western Medicine Cultivation Discipline(No.2019XK159)Guangxi Special Expert Special Project Funding(No.Gui Ren Cai Tong Zi(2019)13)。
文摘microRNA(miRNA)is a type of small non-coding RNA that can participate in cell proliferation and apoptosis by regulating gene expression.More and more evidences indicate that miRNA-200a is involved in the occurrence and development of non-alcoholic fatty liver disease,alcoholic liver disease,drug-induced liver injury,liver fibrosis,and hepatocellular carcinoma.Downstream target genes of serotonin,regulating related signal pathways and playing different roles in the progression of a variety of liver diseases,provide a reference for exploring the mechanism of a variety of chronic liver diseases.
文摘目的总结microRNA-200(miR-200)家族在三阴性乳腺癌(triple-negative breast cancer,TNBC)中的研究进展。方法系统检索并阅读国内外的相关文献,对近年来miR-200家族在TNBC中的研究进展进行综述。结果miR-200家族在TNBC的增殖、侵袭转移及治疗抵抗性中发挥重要作用,并可作为潜在的治疗靶点和生物预测因子。不同的miR-200家族成员及其差异表达介导不同的靶向作用,这可能与信号通路及细胞环境差异有关。结论miR-200家族在TNBC的发生和发展过程中具有关键性的调控作用,这有望为TNBC的治疗及预后评估提供新思路,但其作用机制仍待进一步的深入研究。
