目的探究超声造影联合血清微小RNA(microRNA,miR)-124浓度对急性脑梗死的预测价值及其与颈动脉粥样硬化斑块增强的关系。方法选取2021年2月至2022年10月在秦皇岛市中医医院进行诊断治疗的115例急性脑梗死患者为研究对象,根据患者脑梗死...目的探究超声造影联合血清微小RNA(microRNA,miR)-124浓度对急性脑梗死的预测价值及其与颈动脉粥样硬化斑块增强的关系。方法选取2021年2月至2022年10月在秦皇岛市中医医院进行诊断治疗的115例急性脑梗死患者为研究对象,根据患者脑梗死面积将患者分为小面积组39例(梗死面积<3 cm2),中面积组40例(梗死面积为3~5 cm2),大面积组36例(梗死面积>5 cm2)。另选取同期来本院就诊的合并颈动脉粥样斑块但无急性脑梗死的患者40例为对照组。比较各组患者的达峰时间(time to peak,TTP)、峰值强度比(peak intensity ratio,PIR)、曲线下面积(area under the curve,AUC)和平均通过时间(mean time to passage,MTT),miR124浓度。采用Pearson相关性分析对超声造影参数、血清miR-124浓度与斑块强度的相关性进行分析;采用受试者工作特征曲线(receiver operating characteristic curve,ROC)分析超声造影参数、血清miR-124浓度预测脑梗死的临床价值。结果急性脑梗死组患者TTP、MTT明显短于对照组,PIR、AUC、miR-124浓度明显高于对照组,差异有统计学意义(P<0.05)。中面积、大面积组患者TTP、MTT明显短于小面积组,PIR、AUC、miR-124浓度明显高于小面积组,差异有统计学意义(P<0.05)。大面积组患者TTP、MTT明显短于中面积组,PIR、AUC、miR124浓度明显高于中面积组,差异有统计学意义(P<0.05)。急性脑梗死组患者0级、1级占比明显低于对照组,2级、3级占比明显高于对照组,差异有统计学意义(P<0.05)。Pearson相关性分析显示,颈动脉粥样硬化斑块中超声参数TTP和MTT与斑块增强程度呈负相关(r=-0.388,-0.524,P<0.001)。PIR、AUC、血清miR-124浓度分别与斑块增强程度呈正相关(r=0.487、0.556、0.574,P<0.001)。ROC分析结果显示TTP、PIR、AUC、MTT、miR-124浓度单独预测脑梗死的AUC分别为0.766、0.800、0.761、0.712、0.843,联合检测预测脑梗死的AUC为0.916,联合检测临床价值高于单一指标检测。结论超声造影参数结合血清miR-124浓度可提高急性脑梗死的预测性能,对评估颈动脉粥样硬化斑块的稳定性具有辅助价值。展开更多
Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the stu...Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the study, the exonic and 3’UTR sequences of five genes in 12 sporadic colorectal tumors were extracted by next generation sequencing. In tumors with variation in the 3’UTR region, the changes caused by the variation in the miRNA binding profile were detected. The expression profile of these miRNAs in colorectal and other solid tumors compared to normal tissue was determined. Pathway analysis was performed to determine which signaling pathways miRNAs affect. Results: Case-10 in our study was wild type KRAS and received cetuximab treatment and developed drug resistance. In this case, it was concluded that the expression of KRAS increased and tumorigenesis progressed due to miRNAs that do not bind to this region due to variations in the 3’UTR region. Among these miRNAs, hsa-miR-124-3p was found to have decreased expression in colorectal tumors and to be associated with the ECM-receptor interaction pathway. Conclusion: Variations in the 3’UTR regions of genes critical in the process of carsinogenesis are associated with drug resistance and the process of tumorigenesis.展开更多
文摘目的探究超声造影联合血清微小RNA(microRNA,miR)-124浓度对急性脑梗死的预测价值及其与颈动脉粥样硬化斑块增强的关系。方法选取2021年2月至2022年10月在秦皇岛市中医医院进行诊断治疗的115例急性脑梗死患者为研究对象,根据患者脑梗死面积将患者分为小面积组39例(梗死面积<3 cm2),中面积组40例(梗死面积为3~5 cm2),大面积组36例(梗死面积>5 cm2)。另选取同期来本院就诊的合并颈动脉粥样斑块但无急性脑梗死的患者40例为对照组。比较各组患者的达峰时间(time to peak,TTP)、峰值强度比(peak intensity ratio,PIR)、曲线下面积(area under the curve,AUC)和平均通过时间(mean time to passage,MTT),miR124浓度。采用Pearson相关性分析对超声造影参数、血清miR-124浓度与斑块强度的相关性进行分析;采用受试者工作特征曲线(receiver operating characteristic curve,ROC)分析超声造影参数、血清miR-124浓度预测脑梗死的临床价值。结果急性脑梗死组患者TTP、MTT明显短于对照组,PIR、AUC、miR-124浓度明显高于对照组,差异有统计学意义(P<0.05)。中面积、大面积组患者TTP、MTT明显短于小面积组,PIR、AUC、miR-124浓度明显高于小面积组,差异有统计学意义(P<0.05)。大面积组患者TTP、MTT明显短于中面积组,PIR、AUC、miR124浓度明显高于中面积组,差异有统计学意义(P<0.05)。急性脑梗死组患者0级、1级占比明显低于对照组,2级、3级占比明显高于对照组,差异有统计学意义(P<0.05)。Pearson相关性分析显示,颈动脉粥样硬化斑块中超声参数TTP和MTT与斑块增强程度呈负相关(r=-0.388,-0.524,P<0.001)。PIR、AUC、血清miR-124浓度分别与斑块增强程度呈正相关(r=0.487、0.556、0.574,P<0.001)。ROC分析结果显示TTP、PIR、AUC、MTT、miR-124浓度单独预测脑梗死的AUC分别为0.766、0.800、0.761、0.712、0.843,联合检测预测脑梗死的AUC为0.916,联合检测临床价值高于单一指标检测。结论超声造影参数结合血清miR-124浓度可提高急性脑梗死的预测性能,对评估颈动脉粥样硬化斑块的稳定性具有辅助价值。
文摘Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the study, the exonic and 3’UTR sequences of five genes in 12 sporadic colorectal tumors were extracted by next generation sequencing. In tumors with variation in the 3’UTR region, the changes caused by the variation in the miRNA binding profile were detected. The expression profile of these miRNAs in colorectal and other solid tumors compared to normal tissue was determined. Pathway analysis was performed to determine which signaling pathways miRNAs affect. Results: Case-10 in our study was wild type KRAS and received cetuximab treatment and developed drug resistance. In this case, it was concluded that the expression of KRAS increased and tumorigenesis progressed due to miRNAs that do not bind to this region due to variations in the 3’UTR region. Among these miRNAs, hsa-miR-124-3p was found to have decreased expression in colorectal tumors and to be associated with the ECM-receptor interaction pathway. Conclusion: Variations in the 3’UTR regions of genes critical in the process of carsinogenesis are associated with drug resistance and the process of tumorigenesis.
