Cu–Ni and Cu–Co–Ni superhydrophobic films were constructed on the surface of B10 copper–nickel alloy welded joints using a two-step process of electrodeposition and stearic acid modification.The chemical compositi...Cu–Ni and Cu–Co–Ni superhydrophobic films were constructed on the surface of B10 copper–nickel alloy welded joints using a two-step process of electrodeposition and stearic acid modification.The chemical composition of the film surface was determined using surface characterization techniques.The corrosion resistance of the films was characterized using electrochemical impedance spectroscopy,potentiodynamic polarization,and scanning Kelvin probe microscopy at multiple scales.The thermal stability,mechanical stability,and self-cleaning properties of the films were also characterized.It was determined that the Cu–Co–Ni superhydrophobic film exhibited the best performance,with a static water contact angle of 159.3°,a roll-off angle of 2.3°,a charge transfer resistance 3300 times higher than the substrate,a self-corrosion current density nearly three orders of magnitude lower,and a surface Kelvin potential increase of 420 mV.The film demonstrated good thermal stability,excellent mechanical stability,and outstanding self-cleaning properties.Combining with previous studies,it was found that Co elements in the film contribute to the formation of a uniform and dense film,Ni elements enhance the adhesion and corrosion resistance between the films,and the combination of Co and Ni elements promotes uniform surface potential and further improves the corrosion resistance and interfilm adhesion of the films.展开更多
Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-asso...Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis pigmentosa.The aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal function.To do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and immunofluorescence.Data-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western blotting.AAV2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 mice.Differentially expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type mice.Kyoto Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction pathways.Furthermore,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B treatment.Finally,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B treatment.Our data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upregulating Bcl-2/Bax expression in retinitis pigmentosa.展开更多
基金fnancial support by the National Natural Science Foundation of China(Grant No.42176209)the Natural Science Foundation of Shandong Province(Grant No.ZR2021MD064).
文摘Cu–Ni and Cu–Co–Ni superhydrophobic films were constructed on the surface of B10 copper–nickel alloy welded joints using a two-step process of electrodeposition and stearic acid modification.The chemical composition of the film surface was determined using surface characterization techniques.The corrosion resistance of the films was characterized using electrochemical impedance spectroscopy,potentiodynamic polarization,and scanning Kelvin probe microscopy at multiple scales.The thermal stability,mechanical stability,and self-cleaning properties of the films were also characterized.It was determined that the Cu–Co–Ni superhydrophobic film exhibited the best performance,with a static water contact angle of 159.3°,a roll-off angle of 2.3°,a charge transfer resistance 3300 times higher than the substrate,a self-corrosion current density nearly three orders of magnitude lower,and a surface Kelvin potential increase of 420 mV.The film demonstrated good thermal stability,excellent mechanical stability,and outstanding self-cleaning properties.Combining with previous studies,it was found that Co elements in the film contribute to the formation of a uniform and dense film,Ni elements enhance the adhesion and corrosion resistance between the films,and the combination of Co and Ni elements promotes uniform surface potential and further improves the corrosion resistance and interfilm adhesion of the films.
基金supported by the National Natural Science Foundation of China,Nos.82071008(to BL)and 82004001(to XJ)Medical Science and Technology Program of Health Commission of Henan Province,No.LHGJ20210072(to RQ)Science and Technology Department of Henan Province,No.212102310307(to XJ)。
文摘Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis pigmentosa.The aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal function.To do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and immunofluorescence.Data-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western blotting.AAV2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 mice.Differentially expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type mice.Kyoto Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction pathways.Furthermore,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B treatment.Finally,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B treatment.Our data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upregulating Bcl-2/Bax expression in retinitis pigmentosa.
