Centered on thetechniques and industrial applications of the reinforced cyclonic separation process, its principles and mechanism for separation ot ions, molecules and their aggregates using polyalsperse aroplets are ...Centered on thetechniques and industrial applications of the reinforced cyclonic separation process, its principles and mechanism for separation ot ions, molecules and their aggregates using polyalsperse aroplets are discussed generally; the characteristics and influential factors of fish-hook phenomenon of the grade efficiency curve in cyclonic separation for both gas and liquid are analyzed; and the influence of shear force on particle be- havior （or that of particle swarm） is also summarized. A novel idea for cyclonic separation is presented here： enhancing the cyclonic seoaration process of ions, molecules and their aggregates with monodisperse microspheres and their surface grafting, rearranging the distribution of particles by size using centrifugal field, reinforcing the cyclonic separation performance with orderly arranged particle swarm. Also the investigation of the shortcut flow, recirculation flow, the asymmetric structure and non-linear characteristics of the cyclonic flow field with a com-bined method of Volumetric 3-component Velocimetry （V3V） and Phase-Doppler Particle Anemometer （PDPA） are elaborated. It is recommended to develop new systems for the separation of heterogeneous phases with cyclonic technology, in accordance with the capture and reuse of CO2, methanol to olefins （MTO） process, coal transfer, andthe exploitation of oil shale.展开更多
Dimethyl phthalate(DMP), used as a plasticizer in industrial products, exists widely in air,water and soil.Staphylococcus aureus is a typical model organism representing Gram-positive bacteria.The molecular mechanisms...Dimethyl phthalate(DMP), used as a plasticizer in industrial products, exists widely in air,water and soil.Staphylococcus aureus is a typical model organism representing Gram-positive bacteria.The molecular mechanisms of DMP toxicology in S.aureus were researched by proteomic and transcriptomic analyses.The results showed that the cell wall, membrane and cell surface characteristics were damaged and the growth was inhibited in S.aureus by DMP.Oxidative stress was induced by DMP in S.aureus.The activities of succinic dehydrogenase(SDH) and ATPase were changed by DMP, which could impact energy metabolism.Based on proteomic and transcriptomic analyses, the oxidative phosphorylation pathway was enhanced and the glycolysis/gluconeogenesis and pentose phosphate pathways were inhibited in S.aureus exposed to DMP.The results of real-time reverse transcription quantitative PCR(RT-qPCR) further confirmed the results of the proteomic and transcriptomic analyses.Lactic acid, pyruvic acid and glucose were reduced by DMP in S.aureus, which suggested that DMP could inhibit energy metabolism.The results indicated that DMP damaged the cell wall and membrane, induced oxidative stress, and inhibited energy metabolism and activation in S.aureus.展开更多
基金Supported by the National Natural Science Foundation of China (21076073)the Shanghai Foundation for Development of Science and Technology (10DZ1201300)Sci-Tech Development Plan of SINOPEC (109070)
文摘Centered on thetechniques and industrial applications of the reinforced cyclonic separation process, its principles and mechanism for separation ot ions, molecules and their aggregates using polyalsperse aroplets are discussed generally; the characteristics and influential factors of fish-hook phenomenon of the grade efficiency curve in cyclonic separation for both gas and liquid are analyzed; and the influence of shear force on particle be- havior （or that of particle swarm） is also summarized. A novel idea for cyclonic separation is presented here： enhancing the cyclonic seoaration process of ions, molecules and their aggregates with monodisperse microspheres and their surface grafting, rearranging the distribution of particles by size using centrifugal field, reinforcing the cyclonic separation performance with orderly arranged particle swarm. Also the investigation of the shortcut flow, recirculation flow, the asymmetric structure and non-linear characteristics of the cyclonic flow field with a com-bined method of Volumetric 3-component Velocimetry （V3V） and Phase-Doppler Particle Anemometer （PDPA） are elaborated. It is recommended to develop new systems for the separation of heterogeneous phases with cyclonic technology, in accordance with the capture and reuse of CO2, methanol to olefins （MTO） process, coal transfer, andthe exploitation of oil shale.
基金supported by the National Natural Science Foundation of China (Nos.31870493 and 31670375)the Basic Research Fees of Universities in Heilongjiang Province, China (No.135409103)。
文摘Dimethyl phthalate(DMP), used as a plasticizer in industrial products, exists widely in air,water and soil.Staphylococcus aureus is a typical model organism representing Gram-positive bacteria.The molecular mechanisms of DMP toxicology in S.aureus were researched by proteomic and transcriptomic analyses.The results showed that the cell wall, membrane and cell surface characteristics were damaged and the growth was inhibited in S.aureus by DMP.Oxidative stress was induced by DMP in S.aureus.The activities of succinic dehydrogenase(SDH) and ATPase were changed by DMP, which could impact energy metabolism.Based on proteomic and transcriptomic analyses, the oxidative phosphorylation pathway was enhanced and the glycolysis/gluconeogenesis and pentose phosphate pathways were inhibited in S.aureus exposed to DMP.The results of real-time reverse transcription quantitative PCR(RT-qPCR) further confirmed the results of the proteomic and transcriptomic analyses.Lactic acid, pyruvic acid and glucose were reduced by DMP in S.aureus, which suggested that DMP could inhibit energy metabolism.The results indicated that DMP damaged the cell wall and membrane, induced oxidative stress, and inhibited energy metabolism and activation in S.aureus.
